ID   STX16_MOUSE             Reviewed;         326 AA.
AC   Q8BVI5; A2ACM7;
DT   24-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 3.
DT   27-MAR-2024, entry version 149.
DE   RecName: Full=Syntaxin-16;
GN   Name=Stx16;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Medulla oblongata;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA   Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA   Thibault P.;
RT   "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL   Immunity 30:143-154(2009).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-41 AND SER-74, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung,
RC   Pancreas, Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: SNARE involved in vesicular transport from the late endosomes
CC       to the trans-Golgi network. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with GCC2 (By similarity). Interacts with BAIAP3;
CC       this interaction is increased in the presence of calcium (By
CC       similarity). {ECO:0000250, ECO:0000250|UniProtKB:O14662}.
CC   -!- INTERACTION:
CC       Q8BVI5; O08700: Vps45; Xeno; NbExp=6; IntAct=EBI-3871057, EBI-7849203;
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC       pass type IV membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the syntaxin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC37129.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AK078110; BAC37129.1; ALT_INIT; mRNA.
DR   EMBL; AL669896; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS17147.2; -.
DR   RefSeq; NP_766263.2; NM_172675.4.
DR   AlphaFoldDB; Q8BVI5; -.
DR   SMR; Q8BVI5; -.
DR   IntAct; Q8BVI5; 2.
DR   MINT; Q8BVI5; -.
DR   STRING; 10090.ENSMUSP00000085218; -.
DR   iPTMnet; Q8BVI5; -.
DR   PhosphoSitePlus; Q8BVI5; -.
DR   EPD; Q8BVI5; -.
DR   jPOST; Q8BVI5; -.
DR   MaxQB; Q8BVI5; -.
DR   PaxDb; 10090-ENSMUSP00000085218; -.
DR   PeptideAtlas; Q8BVI5; -.
DR   ProteomicsDB; 254764; -.
DR   Pumba; Q8BVI5; -.
DR   Antibodypedia; 29123; 193 antibodies from 27 providers.
DR   DNASU; 228960; -.
DR   Ensembl; ENSMUST00000087908.10; ENSMUSP00000085218.4; ENSMUSG00000027522.16.
DR   GeneID; 228960; -.
DR   KEGG; mmu:228960; -.
DR   UCSC; uc008oej.1; mouse.
DR   AGR; MGI:1923396; -.
DR   CTD; 8675; -.
DR   MGI; MGI:1923396; Stx16.
DR   VEuPathDB; HostDB:ENSMUSG00000027522; -.
DR   eggNOG; KOG0809; Eukaryota.
DR   GeneTree; ENSGT01000000214440; -.
DR   InParanoid; Q8BVI5; -.
DR   OMA; NRKMCII; -.
DR   OrthoDB; 2876074at2759; -.
DR   PhylomeDB; Q8BVI5; -.
DR   TreeFam; TF314090; -.
DR   Reactome; R-MMU-6811438; Intra-Golgi traffic.
DR   Reactome; R-MMU-6811440; Retrograde transport at the Trans-Golgi-Network.
DR   BioGRID-ORCS; 228960; 2 hits in 76 CRISPR screens.
DR   ChiTaRS; Stx16; mouse.
DR   PRO; PR:Q8BVI5; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; Q8BVI5; Protein.
DR   Bgee; ENSMUSG00000027522; Expressed in manus and 234 other cell types or tissues.
DR   ExpressionAtlas; Q8BVI5; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:MGI.
DR   GO; GO:0031985; C:Golgi cisterna; ISO:MGI.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR   GO; GO:0016020; C:membrane; ISO:MGI.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; ISO:MGI.
DR   GO; GO:0031201; C:SNARE complex; ISO:MGI.
DR   GO; GO:0030672; C:synaptic vesicle membrane; ISO:MGI.
DR   GO; GO:0005802; C:trans-Golgi network; ISO:MGI.
DR   GO; GO:0005484; F:SNAP receptor activity; ISO:MGI.
DR   GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR   GO; GO:0019905; F:syntaxin binding; ISO:MGI.
DR   GO; GO:0032456; P:endocytic recycling; ISO:MGI.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISS:UniProtKB.
DR   GO; GO:0048278; P:vesicle docking; IBA:GO_Central.
DR   GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR   CDD; cd15845; SNARE_syntaxin16; 1.
DR   Gene3D; 1.20.58.70; -; 1.
DR   InterPro; IPR010989; SNARE.
DR   InterPro; IPR045242; Syntaxin.
DR   InterPro; IPR006012; Syntaxin/epimorphin_CS.
DR   InterPro; IPR006011; Syntaxin_N.
DR   InterPro; IPR000727; T_SNARE_dom.
DR   PANTHER; PTHR19957; SYNTAXIN; 1.
DR   PANTHER; PTHR19957:SF83; SYNTAXIN-16; 1.
DR   Pfam; PF05739; SNARE; 1.
DR   Pfam; PF00804; Syntaxin; 1.
DR   SMART; SM00397; t_SNARE; 1.
DR   SUPFAM; SSF47661; t-snare proteins; 1.
DR   PROSITE; PS00914; SYNTAXIN; 1.
DR   PROSITE; PS50192; T_SNARE; 1.
DR   Genevisible; Q8BVI5; MM.
PE   1: Evidence at protein level;
KW   Coiled coil; Golgi apparatus; Membrane; Phosphoprotein; Protein transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..326
FT                   /note="Syntaxin-16"
FT                   /id="PRO_0000210227"
FT   TOPO_DOM        1..302
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        303..323
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        324..326
FT                   /note="Vesicular"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          231..293
FT                   /note="t-SNARE coiled-coil homology"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT   MOD_RES         41
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   MOD_RES         74
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:21183079"
FT   CONFLICT        1
FT                   /note="M -> T (in Ref. 1; BAC37129)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        45..46
FT                   /note="EL -> DV (in Ref. 1; BAC37129)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   326 AA;  37080 MW;  E1042EA1A15372B6 CRC64;
     MATRRLTDAF LLLRNNSIQT RQLLAEQVSS HTTSSPLHSR SIAAELDELA DDRMALVSGI
     SLDPEAAIGV TKRSPPKWVD GVDEIQYDVG RIKQKMKELA SLHDKHLNRP TLDDSSEEEH
     AIEITTQEVT QLFHRCQRAV QALPSRARRA CSEQEERLLR NVVASLAQAL QELSTSFRHA
     QSDYLKRMKN REERSQHFFD TPVPLMDDGD DATLYGQGFT DDQLVLVEQN TLMVEERERE
     IRQIVQSISD LNEIFRDLGA MIVEQGTVLD RIDYNVEQSC VKTEDGLKQL HKAEQYQKKN
     RKMLVIVILV AVIIVLLVAL VAVKSR
//