Reviewed, UniProtKB/Swiss-Prot Q8BVG4 (DPP9_MOUSE)

Names and origin

Protein names

Recommended name:
    Dipeptidyl peptidase 9
    EC=3.4.14.5
Alternative name(s):
    Dipeptidyl peptidase IX
    DP9
    Dipeptidyl peptidase-like protein 9
      Short name=DPLP9

Gene names

Name:

Dpp9

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2 By similarity.
Catalytic activity	Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Enzyme regulation	Inhibited by the serine proteinase inhibitor 4-(2-aminoethyl)benzenesulphonyl fluoride (AEBSF), and by di-isopropylfuorophosphate By similarity.
Subcellular location	Cytoplasm › cytosol By similarity.
Sequence similarities	Belongs to the peptidase S9B family. DPPIV subfamily.
Sequence caution	The sequence BAD21428.1 differs from that shown. Reason: Frameshift at position 745.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Aminopeptidase Hydrolase Protease Serine protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BVG4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BVG4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-75: Missing.
     748-787: VAIAGAPVTV...QGYEAGSVAL → PPHEAESPSS...PGTPASEGQR
     788-862: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 862	862	Dipeptidyl peptidase 9		PRO_0000122416
Sites
	Active site	729	1	Charge relay system By similarity
	Active site	807	1	Charge relay system By similarity
	Active site	839	1	Charge relay system By similarity
Natural variations
	Alternative sequence	1 – 75	75	Missing in isoform 2.		VSP_013870
	Alternative sequence	748 – 787	40	VAIAG…GSVAL → PPHEAESPSSLPATTDPRMA SASSSMWEAKPGTPASEGQR in isoform 2.		VSP_013871
	Alternative sequence	788 – 862	75	Missing in isoform 2.		VSP_013872
Experimental info
	Sequence conflict	369	1	D → Y in BAC37211. Ref.1
	Sequence conflict	546	1	S → F in BAD21428. Ref.2
	Sequence conflict	777	1	Q → K in BAC37211. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: B1D566E824A834E8	FASTA	862	98,001
	10 20 30 40 50 60 MCSGVSPVEQ VAAGDMDDTA ARFCVQKHSW DGLRSIIHGS RKSSGLIVSK APHDFQFVQK 70 80 90 100 110 120 PDESGPHSHR LYYLGMPYGS RENSLLYSEI PKKVRKEALL LLSWKQMLDH FQATPHHGVY 130 140 150 160 170 180 SREEELLRER KRLGVFGITS YDFHSESGLF LFQASNSLFH CRDGGKNGFM VSPMKPLEIK 190 200 210 220 230 240 TQCSGPRMDP KICPADPAFF SFINNSDLWV ANIETGEERR LTFCHQGSAG VLDNPKSAGV 250 260 270 280 290 300 ATFVIQEEFD RFTGCWWCPT ASWEGSEGLK TLRILYEEVD ESEVEVIHVP SPALEERKTD 310 320 330 340 350 360 SYRYPRTGSK NPKIALKLAE LQTDHQGKIV SSCEKELVQP FSSLFPKVEY IARAGWTRDG 370 380 390 400 410 420 KYAWAMFLDR PQQRLQLVLL PPALFIPAVE SEAQRQAAAR AVPKNVQPFV IYEEVTNVWI 430 440 450 460 470 480 NVHDIFHPFP QAEGQQDFCF LRANECKTGF CHLYRVTVEL KTKDYDWTEP LSPTEDEFKC 490 500 510 520 530 540 PIKEEVALTS GEWEVLSRHG SKIWVNEQTK LVYFQGTKDT PLEHHLYVVS YESAGEIVRL 550 560 570 580 590 600 TTLGFSHSCS MSQSFDMFVS HYSSVSTPPC VHVYKLSGPD DDPLHKQPRF WASMMEAANC 610 620 630 640 650 660 PPDYVPPEIF HFHTRADVQL YGMIYKPHTL QPGRKHPTVL FVYGGPQVQL VNNSFKGIKY 670 680 690 700 710 720 LRLNTLASLG YAVVVIDGRG SCQRGLHFEG ALKNQMGQVE IEDQVEGLQY VAEKYGFIDL 730 740 750 760 770 780 SRVAIHGWSY GGFLSLMGLI HKPQVFKVAI AGAPVTVWMA YDTGYTERYM DVPENNQQGY 790 800 810 820 830 840 EAGSVALHVE KLPNEPNRLL ILHGFLDENV HFFHTNFLVS QLIRAGKPYQ LQIYPNERHS 850 860 IRCRESGEHY EVTLLHFLQE HL
	Isoform 2. Checksum: 658A3A4569A5F283	FASTA	712	80,596
	10 20 30 40 50 60 MPYGSRENSL LYSEIPKKVR KEALLLLSWK QMLDHFQATP HHGVYSREEE LLRERKRLGV 70 80 90 100 110 120 FGITSYDFHS ESGLFLFQAS NSLFHCRDGG KNGFMVSPMK PLEIKTQCSG PRMDPKICPA 130 140 150 160 170 180 DPAFFSFINN SDLWVANIET GEERRLTFCH QGSAGVLDNP KSAGVATFVI QEEFDRFTGC 190 200 210 220 230 240 WWCPTASWEG SEGLKTLRIL YEEVDESEVE VIHVPSPALE ERKTDSYRYP RTGSKNPKIA 250 260 270 280 290 300 LKLAELQTDH QGKIVSSCEK ELVQPFSSLF PKVEYIARAG WTRDGKYAWA MFLDRPQQRL 310 320 330 340 350 360 QLVLLPPALF IPAVESEAQR QAAARAVPKN VQPFVIYEEV TNVWINVHDI FHPFPQAEGQ 370 380 390 400 410 420 QDFCFLRANE CKTGFCHLYR VTVELKTKDY DWTEPLSPTE DEFKCPIKEE VALTSGEWEV 430 440 450 460 470 480 LSRHGSKIWV NEQTKLVYFQ GTKDTPLEHH LYVVSYESAG EIVRLTTLGF SHSCSMSQSF 490 500 510 520 530 540 DMFVSHYSSV STPPCVHVYK LSGPDDDPLH KQPRFWASMM EAANCPPDYV PPEIFHFHTR 550 560 570 580 590 600 ADVQLYGMIY KPHTLQPGRK HPTVLFVYGG PQVQLVNNSF KGIKYLRLNT LASLGYAVVV 610 620 630 640 650 660 IDGRGSCQRG LHFEGALKNQ MGQVEIEDQV EGLQYVAEKY GFIDLSRVAI HGWSYGGFLS 670 680 690 700 710 LMGLIHKPQV FKPPHEAESP SSLPATTDPR MASASSSMWE AKPGTPASEG QR

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Liver and Olfactory bulb.
[2]	"Prediction of the coding sequences of mouse homologues of FLJ genes: the complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H. DNA Res. 11:127-135(2004) [PubMed: 15449545] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Salivary gland.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK050021 mRNA. Translation: BAC34034.1. AK078301 mRNA. Translation: BAC37211.1. AK131178 mRNA. Translation: BAD21428.1. Sequence problems. BC057631 mRNA. Translation: AAH57631.1.
IPI	IPI00462461. IPI00606731.
RefSeq	NP_766212.1.
UniGene	Mm.205535
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S09.019.
Proteomic databases
PRIDE	Q8BVG4.
Genome annotation databases
Ensembl	ENSMUSG00000001229. Mus musculus. [Contig view]
GeneID	224897.
KEGG	mmu:224897.
Organism-specific databases
MGI	MGI:2443967. Dpp9.
Phylogenomic databases
HOGENOM	Q8BVG4.
HOVERGEN	Q8BVG4.
OMA	Q8BVG4. RHGSKIW.
Enzyme and pathway databases
BRENDA	3.4.14.5. 244.
Gene expression databases
ArrayExpress	Q8BVG4.
Bgee	Q8BVG4.
CleanEx	MM_DPP9.
GermOnline	ENSMUSG00000001229. Mus musculus.
Family and domain databases
InterPro	IPR001375. Peptidase_S9. IPR002469. Peptidase_S9B. [Graphical view]
Pfam	PF00930. DPPIV_N. 1 hit. PF00326. Peptidase_S9. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	377440.
SOURCE	Search...

Entry information

Entry name

DPP9_MOUSE

Accession

Primary (citable) accession number: Q8BVG4
Secondary accession number(s): Q6KAM9, Q8BWT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)