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Q8BUR4 (DOCK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dedicator of cytokinesis protein 1
Alternative name(s):
180 kDa protein downstream of CRK
Short name=DOCK180
Gene names
Name:Dock1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1865 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1 By similarity.

Subunit structure

Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 By similarity. Interacts with ELMO1 and BAI1. Ref.5

Subcellular location

Cytoplasm By similarity. Membrane By similarity. Note: Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate By similarity.

Domain

The DHR-2 domain is necessary and sufficient for the GEF activity.

Sequence similarities

Belongs to the DOCK family.

Contains 1 DHR-1 domain.

Contains 1 DHR-2 domain.

Contains 1 SH3 domain.

Binary interactions

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BUR4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BUR4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1572-1572: I → V
     1573-1865: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 18651865Dedicator of cytokinesis protein 1
PRO_0000189985

Regions

Domain9 – 7062SH3
Domain425 – 609185DHR-1
Domain1207 – 1617411DHR-2
Region1687 – 16959Phosphoinositide-binding Potential
Region1793 – 181927Interaction with NCK2 second and third SH3 domain (minor) By similarity
Region1820 – 183617Interaction with NCK2 third SH3 domain (major) By similarity
Region1837 – 185216Interaction with NCK2 (minor) By similarity
Motif1799 – 18057SH3-binding; interaction with CRK Potential
Motif1838 – 18436SH3-binding; interaction with CRK Potential

Amino acid modifications

Modified residue17561Phosphoserine

Natural variations

Alternative sequence15721I → V in isoform 2.
VSP_022205
Alternative sequence1573 – 1865293Missing in isoform 2.
VSP_022206

Experimental info

Sequence conflict13581I → V in BAE22333. Ref.1

Secondary structure

................ 1865
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 9, 2007. Version 3.
Checksum: 4FFEAA4CBED8DE62

FASTA1,865215,085
        10         20         30         40         50         60 
MTRWVPTKRE EKYGVAFYNY DARGADELSL QIGDTVHILE TYEGWYRGYT LRKKSKKGIF 

        70         80         90        100        110        120 
PASYIHLKEA IVEGKGQHET VIPGDLPLIQ EVTTTLREWS TIWRQLYVQD NREMFRSVRH 

       130        140        150        160        170        180 
MIYDLIEWRS QILSGTLPQD ELKELKKKVT AKIDYGNRIL DLDLVVRDED GNILDPELTS 

       190        200        210        220        230        240 
TISLFRAHEV ASKQVEERLQ EEKSQKQNMD INRQAKFAAT PSLALFVNLK NVVCKIGEDA 

       250        260        270        280        290        300 
EVLMSLYDPM ESKFISENYL VRWSSSGLPK DIDRLHNLRA VFTDLGSKDL KREKISFVCQ 

       310        320        330        340        350        360 
IVRVGRMELR DSNTRKLTSG LRRPFGVAVM DVTDIINGKV DDEDKQHFIP FQAVAGENDF 

       370        380        390        400        410        420 
LQTVINKVIA AKEVNHKGQG LWVTLKLLPG DIHQIRKEFP HLVDRTTAVA RKTGFPEIIM 

       430        440        450        460        470        480 
PGDVRNDIYV TLVQGDFDKG SKTTAKNVEV TVSVYDEDGK RLEHVIFPGA GDEAISEYKS 

       490        500        510        520        530        540 
VIYYQVKQPR WFETLKVAIP IEDVNRSHLR FTFRHRSSQD SKDKSEKIFA LAFVKLMRYD 

       550        560        570        580        590        600 
GTTLRDGEHD LIVYKAEAKK LEDAATYLSL PSTKGELEEK GHSATGKGMQ SLGSCTISKD 

       610        620        630        640        650        660 
SFQISTLVCS TKLTQNVDLL GLLKWRSNTN LLQQNLRQLM KVDGGEVVKF LQDTLDALFN 

       670        680        690        700        710        720 
IMMENSESET FDTLVFDALV FIIGLIADRK FQHFNPVLET YIKKHFSATL AYTKLTKVLR 

       730        740        750        760        770        780 
TYVASAEKPG VNEQLYKAIK ALEYIFKFIV RSRVLFNQLY ENKGEADFVE SLLQLFRSIN 

       790        800        810        820        830        840 
DMMSSLSELT VRVKGAALKY LPTIVNDVKL VFDPKELSKM FTEFILNVPA GLLTVQKLSC 

       850        860        870        880        890        900 
LIEIVHSDLF TQHDCREILL PMMTDQLKYH LERQEELEAC CQLLSNILEV LYRKDVGPTQ 

       910        920        930        940        950        960 
RHVQIIMETL LRTVNRTVIS MGRDSELIGN FVACMTAILR QMEDYHYAHL IKTFGKMRTD 

       970        980        990       1000       1010       1020 
VVDFLMETFI MFKNLIGKNV YPFDWVIMNT MQNKVFLRAI NQYADMLNKR FLDQANFELQ 

      1030       1040       1050       1060       1070       1080 
LWNNYFHLAV AFLTQESLQL ENFSSAKRAK ILNKYGDMRR QIGFEIRDMW YNLGQHKIKF 

      1090       1100       1110       1120       1130       1140 
IPEMVGPILE MTLIPETELR KATLPIFFDM MQCEFHSTRS FQMFENEIIT KLDHEVEGGR 

      1150       1160       1170       1180       1190       1200 
GDEQYKVLFD KILLEHCRKH KYLAKTGETF VKLVVRLMER LLDYRTIMHD ENKDNRMSCT 

      1210       1220       1230       1240       1250       1260 
VNVLNFYKEI EREEMYIRYL YKLCDLHKEC DNYTEAAYTL LLHAKLLKWS EDVCAAHLTQ 

      1270       1280       1290       1300       1310       1320 
RDGFQATTQG QLKEQLYQEI IHYFDKGKMW EEAIALGKEL AEQYETEMFD YEQLSELLKK 

      1330       1340       1350       1360       1370       1380 
QAQFYENIVK VIRPKPDYFA VGYYGQGFPS FLRGKVFIYR GKEYERREDF EARLLTQFPN 

      1390       1400       1410       1420       1430       1440 
AEKMKTTSPP GDDIKTSPGQ YIQCFTVKPK LDLPPRFHRP VSEQIVSFYR VNEVQRFEYS 

      1450       1460       1470       1480       1490       1500 
RPIRKGEKNP DNEFANMWIE RTIYTTAYKL PGILRWFEVK SVFMVEISPL ENAIETMQLT 

      1510       1520       1530       1540       1550       1560 
NDKISSMVQQ HLDDPGLPIN PLSMLLNGIV DPAVMGGFAN YEKAFFTDRY LQEHPEAHGQ 

      1570       1580       1590       1600       1610       1620 
IEKLKDLIAW QIPFLAEGIR IHGDKVTEAL RPFHERMEAC FKQLKEKVEK QYGVRTMPSG 

      1630       1640       1650       1660       1670       1680 
LDDRRGSRPR SMVRSFTMPS SSRPLSVASV SSFSSDSTPS RPGSDGFALE PLLPKKMHSR 

      1690       1700       1710       1720       1730       1740 
SQDKLDKDDP DKEKKDKKKE KRNSKHQEIF DKEFKPADSS LQQSEAVILS ETISPLRPQR 

      1750       1760       1770       1780       1790       1800 
PKSQVINVIG NERRFSVSPA SPSSQQTPPP VTPRAKLSFS IQPSLELNGM MGMDVADVPP 

      1810       1820       1830       1840       1850       1860 
PLPLKGNMAD YGNLMENQDM MVSPTSPPPP PPQRQQPPPL PSKTPPPPPP KTTRKQTSVD 


SGIVQ 

« Hide

Isoform 2 [UniParc].

Checksum: 11CB0C8127A7573F
Show »

FASTA1,572182,628

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-289 AND 1763-1865 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1171-1865 (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Olfactory bulb.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 902-1865 (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain, Eye and Mammary tumor.
[4]"Evidence that DOCK180 up-regulates signals from the CrkII-p130(Cas) complex."
Kiyokawa E., Hashimoto Y., Kurata T., Sugimura H., Matsuda M.
J. Biol. Chem. 273:24479-24484(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION.
[5]"BAI1 is an engulfment receptor for apoptotic cells upstream of the ELMO/Dock180/Rac module."
Park D., Tosello-Trampont A.-C., Elliott M.R., Lu M., Haney L.B., Ma Z., Klibanov A.L., Mandell J.W., Ravichandran K.S.
Nature 450:430-434(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH BAI1 AND DOCK1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC100208 Genomic DNA. No translation available.
AC127574 Genomic DNA. No translation available.
AC129300 Genomic DNA. No translation available.
AC136515 Genomic DNA. No translation available.
AC136641 Genomic DNA. No translation available.
AK078899 mRNA. Translation: BAC37448.1.
AK082835 mRNA. Translation: BAC38645.1.
AK134905 mRNA. Translation: BAE22333.1.
AK141224 mRNA. Translation: BAE24599.1.
BC006755 mRNA. Translation: AAH06755.1.
BC009668 mRNA. Translation: AAH09668.1.
BC058998 mRNA. Translation: AAH58998.1.
BC098214 mRNA. Translation: AAH98214.1.
CCDSCCDS40166.1. [Q8BUR4-1]
RefSeqNP_001028592.1. NM_001033420.2. [Q8BUR4-1]
UniGeneMm.436572.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2M0YNMR-A1-74[»]
ProteinModelPortalQ8BUR4.
SMRQ8BUR4. Positions 1-168, 425-611, 1192-1617.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8BUR4. 7 interactions.
MINTMINT-4093373.
STRING10090.ENSMUSP00000081531.

PTM databases

PhosphoSiteQ8BUR4.

Proteomic databases

MaxQBQ8BUR4.
PaxDbQ8BUR4.
PRIDEQ8BUR4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000084488; ENSMUSP00000081531; ENSMUSG00000058325. [Q8BUR4-1]
GeneID330662.
KEGGmmu:330662.
UCSCuc009kdu.1. mouse. [Q8BUR4-2]
uc009kdv.1. mouse. [Q8BUR4-1]

Organism-specific databases

CTD1793.
MGIMGI:2429765. Dock1.

Phylogenomic databases

eggNOGNOG289808.
GeneTreeENSGT00610000085806.
HOVERGENHBG051389.
InParanoidQ8BUR4.
KOK13708.
OMANYVVKWS.
OrthoDBEOG7QNVK8.
PhylomeDBQ8BUR4.
TreeFamTF300423.

Gene expression databases

BgeeQ8BUR4.
CleanExMM_DOCK1.
GenevestigatorQ8BUR4.

Family and domain databases

InterProIPR016024. ARM-type_fold.
IPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR010703. DOCK_C.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR23317. PTHR23317. 1 hit.
PfamPF06920. Ded_cyto. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 4 hits.
SSF50044. SSF50044. 1 hit.
PROSITEPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDOCK1. mouse.
NextBio399497.
PROQ8BUR4.
SOURCESearch...

Entry information

Entry nameDOCK1_MOUSE
AccessionPrimary (citable) accession number: Q8BUR4
Secondary accession number(s): Q3URT8 expand/collapse secondary AC list , Q3UY80, Q4QQL8, Q6PD14, Q8BVC9, Q91Z55, Q922V1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot