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Protein

Ubiquitin carboxyl-terminal hydrolase 43

Gene

Usp43

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei110 – 1101NucleophilePROSITE-ProRule annotation
Active sitei668 – 6681Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: GO_Central
  2. ubiquitin-specific protease activity Source: GO_Central

GO - Biological processi

  1. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  2. protein deubiquitination Source: GO_Central
  3. regulation of proteasomal protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 43 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 43
Ubiquitin thioesterase 43
Ubiquitin-specific-processing protease 43
Gene namesi
Name:Usp43
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2444541. Usp43.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11321132Ubiquitin carboxyl-terminal hydrolase 43PRO_0000249522Add
BLAST

Proteomic databases

PRIDEiQ8BUM9.

PTM databases

PhosphoSiteiQ8BUM9.

Expressioni

Gene expression databases

BgeeiQ8BUM9.
CleanExiMM_USP43.
ExpressionAtlasiQ8BUM9. baseline.
GenevestigatoriQ8BUM9.

Structurei

3D structure databases

ProteinModelPortaliQ8BUM9.
SMRiQ8BUM9. Positions 99-273, 539-711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 710610USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000293426.
HOVERGENiHBG094126.
InParanoidiQ8BUM9.
KOiK11856.
OMAiQQCILSK.
OrthoDBiEOG7VMP4B.
PhylomeDBiQ8BUM9.
TreeFamiTF106278.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BUM9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPGVGNALG EGPPAPRPRR RRSLRRLLNR FLLALGSRSR SGDSPPRPPA
60 70 80 90 100
QPSPYDGDGE GGFACAPGPA PASAGSPGPD RPPGSQPQIS SGDGARPPGA
110 120 130 140 150
QGLKNHGNTC FMNAVVQCLS NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL
160 170 180 190 200
VRALWTREYT PQLSAEFKNA VSKYGSQFQG NSQHDALEFL LWLLDRVHED
210 220 230 240 250
LEGSAHGLVS EQLPPEVSKI SEDLRPSAAP TSLGPSFVQS HFQAQYRSSL
260 270 280 290 300
TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV
310 320 330 340 350
PILSTVAALR KMVAEEGGVP AEEVILVELY PNGFQRSFFD EEDLNTIAEG
360 370 380 390 400
DNVYAFQVPP SPGLGTLSAH PSGLSVSPRL PVRDSQRFSG PLHSENRVVF
410 420 430 440 450
LFCNLVGSGQ QASRFGPPFL IREDRTISWA QLQQCILSKV RCLMRSEVSA
460 470 480 490 500
QDLGTLFSIR VVGLSLACSY LSPKDNRPLC HWAVDRALHL RRPGGPPHVK
510 520 530 540 550
LAVEWDSSVT ERLFGSLQEE RVQDADSVWR QQQAHQQPSC TLDECFQSYT
560 570 580 590 600
KEEQLAQDDA WKCPHCQVLQ QGVVKLSLWT LPDILIIHLK RFCQVGERRN
610 620 630 640 650
KLSTLVKFPL SGLNMAPHVA RRSTNSKAGP GPWSSWKQPI CLPTTYPLDF
660 670 680 690 700
LYDLYAVCNH HGSLQGGHYT AYCRNSLDGQ WYSYDDSTVE ALREDEVNTR
710 720 730 740 750
GAYILFYQKR NSIPPWSASS SMRGSTSSSL SDHWLMRLGS LNNSTRGSLL
760 770 780 790 800
SWSSAPCPSM ARVPDSPVFT NGVCHQDKGG VETRPLVRGV GGRSISMKAS
810 820 830 840 850
PASRSRHGPF KTMPLRWSFG HREKRPGASV ELVEYLESRR RPRSTSQSIV
860 870 880 890 900
PLLTRAAGGE ETSASPRSDG TLPAKSEDSG RAIGQGTTGV PLSSCHLNHH
910 920 930 940 950
PALGSLDDSL HTARTRTGNV SQDIRLPKKF DLPLTVMPSV GDEKPARPEG
960 970 980 990 1000
QKMTPWKGSS QVGSQSSPPS PSTGLLRNFK DSGPGTLPKM KSKAAMEERA
1010 1020 1030 1040 1050
PDKDRGQGTF TLLKSVFWKK EHKRTVRTES SPPAPPISLG SDRLSPAAMN
1060 1070 1080 1090 1100
EQALRIRESP AKGLGNHMER DIRSAPSSLH LPRKASRPPR ASTAGTSQRT
1110 1120 1130
IPGEQISYGT LQRVKYHTLS LGRKKSLPES SF
Length:1,132
Mass (Da):123,874
Last modified:September 18, 2006 - v2
Checksum:i3E9E9E46B75E67FA
GO
Isoform 2 (identifier: Q8BUM9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1047-1047: Missing.
     1052-1052: Q → QARGSSP

Note: No experimental confirmation available.

Show »
Length:1,137
Mass (Da):124,359
Checksum:i8EBE4A6ED6AD5AFB
GO

Sequence cautioni

The sequence AAH21474.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAC38837.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti760 – 7601M → V in AAH21474 (PubMed:15489334).Curated
Sequence conflicti982 – 9821S → N in AAH21474 (PubMed:15489334).Curated
Sequence conflicti1002 – 10021D → G in AAH21474 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1047 – 10471Missing in isoform 2. 1 PublicationVSP_020469
Alternative sequencei1052 – 10521Q → QARGSSP in isoform 2. 1 PublicationVSP_020470

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732570 Genomic DNA. Translation: CAI52000.2.
AK083271 mRNA. Translation: BAC38837.1. Different initiation.
BC021474 mRNA. Translation: AAH21474.1. Different initiation.
CCDSiCCDS24861.2. [Q8BUM9-1]
RefSeqiNP_001277978.1. NM_001291049.1.
NP_776115.2. NM_173754.4. [Q8BUM9-1]
UniGeneiMm.158885.

Genome annotation databases

EnsembliENSMUST00000021288; ENSMUSP00000021288; ENSMUSG00000020905. [Q8BUM9-1]
GeneIDi216835.
KEGGimmu:216835.
UCSCiuc007jne.1. mouse. [Q8BUM9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL732570 Genomic DNA. Translation: CAI52000.2.
AK083271 mRNA. Translation: BAC38837.1. Different initiation.
BC021474 mRNA. Translation: AAH21474.1. Different initiation.
CCDSiCCDS24861.2. [Q8BUM9-1]
RefSeqiNP_001277978.1. NM_001291049.1.
NP_776115.2. NM_173754.4. [Q8BUM9-1]
UniGeneiMm.158885.

3D structure databases

ProteinModelPortaliQ8BUM9.
SMRiQ8BUM9. Positions 99-273, 539-711.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8BUM9.

Proteomic databases

PRIDEiQ8BUM9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021288; ENSMUSP00000021288; ENSMUSG00000020905. [Q8BUM9-1]
GeneIDi216835.
KEGGimmu:216835.
UCSCiuc007jne.1. mouse. [Q8BUM9-1]

Organism-specific databases

CTDi124739.
MGIiMGI:2444541. Usp43.

Phylogenomic databases

eggNOGiCOG5560.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000293426.
HOVERGENiHBG094126.
InParanoidiQ8BUM9.
KOiK11856.
OMAiQQCILSK.
OrthoDBiEOG7VMP4B.
PhylomeDBiQ8BUM9.
TreeFamiTF106278.

Miscellaneous databases

NextBioi375380.
PROiQ8BUM9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BUM9.
CleanExiMM_USP43.
ExpressionAtlasiQ8BUM9. baseline.
GenevestigatoriQ8BUM9.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 21-1132 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Hippocampus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 207-1132 (ISOFORM 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiUBP43_MOUSE
AccessioniPrimary (citable) accession number: Q8BUM9
Secondary accession number(s): Q8VDP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2006
Last sequence update: September 18, 2006
Last modified: March 3, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.