Q8BUM9 (UBP43_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 43
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 43
Ubiquitin thioesterase 43
Ubiquitin-specific-processing protease 43

Gene names

Name:

Usp43

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1132 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family.
Sequence caution	The sequence AAH21474.1 differs from that shown. Reason: Erroneous initiation. The sequence BAC38837.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BUM9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BUM9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1047-1047: Missing.
     1052-1052: Q → QARGSSP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1132	1132	Ubiquitin carboxyl-terminal hydrolase 43		PRO_0000249522
Sites
	Active site	110	1	Nucleophile By similarity
	Active site	668	1	Proton acceptor By similarity
Natural variations
	Alternative sequence	1047	1	Missing in isoform 2.		VSP_020469
	Alternative sequence	1052	1	Q → QARGSSP in isoform 2.		VSP_020470
Experimental info
	Sequence conflict	760	1	M → V in AAH21474. Ref.3
	Sequence conflict	982	1	S → N in AAH21474. Ref.3
	Sequence conflict	1002	1	D → G in AAH21474. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 19, 2006. Version 2. Checksum: 3E9E9E46B75E67FA	FASTA	1,132	123,874
	10 20 30 40 50 60 MDPGVGNALG EGPPAPRPRR RRSLRRLLNR FLLALGSRSR SGDSPPRPPA QPSPYDGDGE 70 80 90 100 110 120 GGFACAPGPA PASAGSPGPD RPPGSQPQIS SGDGARPPGA QGLKNHGNTC FMNAVVQCLS 130 140 150 160 170 180 NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL VRALWTREYT PQLSAEFKNA VSKYGSQFQG 190 200 210 220 230 240 NSQHDALEFL LWLLDRVHED LEGSAHGLVS EQLPPEVSKI SEDLRPSAAP TSLGPSFVQS 250 260 270 280 290 300 HFQAQYRSSL TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV 310 320 330 340 350 360 PILSTVAALR KMVAEEGGVP AEEVILVELY PNGFQRSFFD EEDLNTIAEG DNVYAFQVPP 370 380 390 400 410 420 SPGLGTLSAH PSGLSVSPRL PVRDSQRFSG PLHSENRVVF LFCNLVGSGQ QASRFGPPFL 430 440 450 460 470 480 IREDRTISWA QLQQCILSKV RCLMRSEVSA QDLGTLFSIR VVGLSLACSY LSPKDNRPLC 490 500 510 520 530 540 HWAVDRALHL RRPGGPPHVK LAVEWDSSVT ERLFGSLQEE RVQDADSVWR QQQAHQQPSC 550 560 570 580 590 600 TLDECFQSYT KEEQLAQDDA WKCPHCQVLQ QGVVKLSLWT LPDILIIHLK RFCQVGERRN 610 620 630 640 650 660 KLSTLVKFPL SGLNMAPHVA RRSTNSKAGP GPWSSWKQPI CLPTTYPLDF LYDLYAVCNH 670 680 690 700 710 720 HGSLQGGHYT AYCRNSLDGQ WYSYDDSTVE ALREDEVNTR GAYILFYQKR NSIPPWSASS 730 740 750 760 770 780 SMRGSTSSSL SDHWLMRLGS LNNSTRGSLL SWSSAPCPSM ARVPDSPVFT NGVCHQDKGG 790 800 810 820 830 840 VETRPLVRGV GGRSISMKAS PASRSRHGPF KTMPLRWSFG HREKRPGASV ELVEYLESRR 850 860 870 880 890 900 RPRSTSQSIV PLLTRAAGGE ETSASPRSDG TLPAKSEDSG RAIGQGTTGV PLSSCHLNHH 910 920 930 940 950 960 PALGSLDDSL HTARTRTGNV SQDIRLPKKF DLPLTVMPSV GDEKPARPEG QKMTPWKGSS 970 980 990 1000 1010 1020 QVGSQSSPPS PSTGLLRNFK DSGPGTLPKM KSKAAMEERA PDKDRGQGTF TLLKSVFWKK 1030 1040 1050 1060 1070 1080 EHKRTVRTES SPPAPPISLG SDRLSPAAMN EQALRIRESP AKGLGNHMER DIRSAPSSLH 1090 1100 1110 1120 1130 LPRKASRPPR ASTAGTSQRT IPGEQISYGT LQRVKYHTLS LGRKKSLPES SF
	Isoform 2 [UniParc]. Checksum: 8EBE4A6ED6AD5AFB	FASTA	1,137	124,359
	10 20 30 40 50 60 MDPGVGNALG EGPPAPRPRR RRSLRRLLNR FLLALGSRSR SGDSPPRPPA QPSPYDGDGE 70 80 90 100 110 120 GGFACAPGPA PASAGSPGPD RPPGSQPQIS SGDGARPPGA QGLKNHGNTC FMNAVVQCLS 130 140 150 160 170 180 NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL VRALWTREYT PQLSAEFKNA VSKYGSQFQG 190 200 210 220 230 240 NSQHDALEFL LWLLDRVHED LEGSAHGLVS EQLPPEVSKI SEDLRPSAAP TSLGPSFVQS 250 260 270 280 290 300 HFQAQYRSSL TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV 310 320 330 340 350 360 PILSTVAALR KMVAEEGGVP AEEVILVELY PNGFQRSFFD EEDLNTIAEG DNVYAFQVPP 370 380 390 400 410 420 SPGLGTLSAH PSGLSVSPRL PVRDSQRFSG PLHSENRVVF LFCNLVGSGQ QASRFGPPFL 430 440 450 460 470 480 IREDRTISWA QLQQCILSKV RCLMRSEVSA QDLGTLFSIR VVGLSLACSY LSPKDNRPLC 490 500 510 520 530 540 HWAVDRALHL RRPGGPPHVK LAVEWDSSVT ERLFGSLQEE RVQDADSVWR QQQAHQQPSC 550 560 570 580 590 600 TLDECFQSYT KEEQLAQDDA WKCPHCQVLQ QGVVKLSLWT LPDILIIHLK RFCQVGERRN 610 620 630 640 650 660 KLSTLVKFPL SGLNMAPHVA RRSTNSKAGP GPWSSWKQPI CLPTTYPLDF LYDLYAVCNH 670 680 690 700 710 720 HGSLQGGHYT AYCRNSLDGQ WYSYDDSTVE ALREDEVNTR GAYILFYQKR NSIPPWSASS 730 740 750 760 770 780 SMRGSTSSSL SDHWLMRLGS LNNSTRGSLL SWSSAPCPSM ARVPDSPVFT NGVCHQDKGG 790 800 810 820 830 840 VETRPLVRGV GGRSISMKAS PASRSRHGPF KTMPLRWSFG HREKRPGASV ELVEYLESRR 850 860 870 880 890 900 RPRSTSQSIV PLLTRAAGGE ETSASPRSDG TLPAKSEDSG RAIGQGTTGV PLSSCHLNHH 910 920 930 940 950 960 PALGSLDDSL HTARTRTGNV SQDIRLPKKF DLPLTVMPSV GDEKPARPEG QKMTPWKGSS 970 980 990 1000 1010 1020 QVGSQSSPPS PSTGLLRNFK DSGPGTLPKM KSKAAMEERA PDKDRGQGTF TLLKSVFWKK 1030 1040 1050 1060 1070 1080 EHKRTVRTES SPPAPPISLG SDRLSPAMNE QARGSSPALR IRESPAKGLG NHMERDIRSA 1090 1100 1110 1120 1130 PSSLHLPRKA SRPPRASTAG TSQRTIPGEQ ISYGTLQRVK YHTLSLGRKK SLPESSF

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 21-1132 (ISOFORM 1). Strain: C57BL/6J. Tissue: Hippocampus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 207-1132 (ISOFORM 2). Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL732570 Genomic DNA. Translation: CAI52000.2. AK083271 mRNA. Translation: BAC38837.1. Different initiation. BC021474 mRNA. Translation: AAH21474.1. Different initiation.
IPI	IPI00225521. IPI00788336.
RefSeq	NP_776115.2. NM_173754.4.
UniGene	Mm.158885.
3D structure databases
HSSP	HSSP built from PDB template 2GFO based on UniProtKB P40818.
ProteinModelPortal	Q8BUM9.
SMR	Q8BUM9. Positions 99-273, 539-711.
ModBase	Search...
Protein family/group databases
MEROPS	C19.979.
PTM databases
PhosphoSite	Q8BUM9.
Proteomic databases
PRIDE	Q8BUM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021288; ENSMUSP00000021288; ENSMUSG00000020905.
GeneID	216835.
KEGG	mmu:216835.
UCSC	uc007jne.1. mouse.
Organism-specific databases
CTD	124739.
MGI	MGI:2444541. Usp43.
Phylogenomic databases
eggNOG	COG5560.
GeneTree	ENSGT00680000099843.
HOGENOM	HOG000293426.
HOVERGEN	HBG094126.
InParanoid	Q8BUM9.
KO	K11856.
OMA	RSISMKA.
OrthoDB	EOG4DR9BH.
Gene expression databases
ArrayExpress	Q8BUM9.
Bgee	Q8BUM9.
CleanEx	MM_USP43.
Genevestigator	Q8BUM9.
GermOnline	ENSMUSG00000020905. Mus musculus.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	375380.
SOURCE	Search...

Entry information

Entry name

UBP43_MOUSE

Accession

Primary (citable) accession number: Q8BUM9
Secondary accession number(s): Q8VDP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	September 19, 2006
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program