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Q8BUM9 (UBP43_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 43

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 43
Ubiquitin thioesterase 43
Ubiquitin-specific-processing protease 43
Gene names
Name:Usp43
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1132 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence caution

The sequence AAH21474.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAC38837.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Coding sequence diversityAlternative splicing
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncysteine-type peptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BUM9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BUM9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1047-1047: Missing.
     1052-1052: Q → QARGSSP
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11321132Ubiquitin carboxyl-terminal hydrolase 43
PRO_0000249522

Regions

Domain101 – 710610USP

Sites

Active site1101Nucleophile By similarity
Active site6681Proton acceptor By similarity

Natural variations

Alternative sequence10471Missing in isoform 2.
VSP_020469
Alternative sequence10521Q → QARGSSP in isoform 2.
VSP_020470

Experimental info

Sequence conflict7601M → V in AAH21474. Ref.3
Sequence conflict9821S → N in AAH21474. Ref.3
Sequence conflict10021D → G in AAH21474. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 19, 2006. Version 2.
Checksum: 3E9E9E46B75E67FA

FASTA1,132123,874
        10         20         30         40         50         60 
MDPGVGNALG EGPPAPRPRR RRSLRRLLNR FLLALGSRSR SGDSPPRPPA QPSPYDGDGE 

        70         80         90        100        110        120 
GGFACAPGPA PASAGSPGPD RPPGSQPQIS SGDGARPPGA QGLKNHGNTC FMNAVVQCLS 

       130        140        150        160        170        180 
NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL VRALWTREYT PQLSAEFKNA VSKYGSQFQG 

       190        200        210        220        230        240 
NSQHDALEFL LWLLDRVHED LEGSAHGLVS EQLPPEVSKI SEDLRPSAAP TSLGPSFVQS 

       250        260        270        280        290        300 
HFQAQYRSSL TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV 

       310        320        330        340        350        360 
PILSTVAALR KMVAEEGGVP AEEVILVELY PNGFQRSFFD EEDLNTIAEG DNVYAFQVPP 

       370        380        390        400        410        420 
SPGLGTLSAH PSGLSVSPRL PVRDSQRFSG PLHSENRVVF LFCNLVGSGQ QASRFGPPFL 

       430        440        450        460        470        480 
IREDRTISWA QLQQCILSKV RCLMRSEVSA QDLGTLFSIR VVGLSLACSY LSPKDNRPLC 

       490        500        510        520        530        540 
HWAVDRALHL RRPGGPPHVK LAVEWDSSVT ERLFGSLQEE RVQDADSVWR QQQAHQQPSC 

       550        560        570        580        590        600 
TLDECFQSYT KEEQLAQDDA WKCPHCQVLQ QGVVKLSLWT LPDILIIHLK RFCQVGERRN 

       610        620        630        640        650        660 
KLSTLVKFPL SGLNMAPHVA RRSTNSKAGP GPWSSWKQPI CLPTTYPLDF LYDLYAVCNH 

       670        680        690        700        710        720 
HGSLQGGHYT AYCRNSLDGQ WYSYDDSTVE ALREDEVNTR GAYILFYQKR NSIPPWSASS 

       730        740        750        760        770        780 
SMRGSTSSSL SDHWLMRLGS LNNSTRGSLL SWSSAPCPSM ARVPDSPVFT NGVCHQDKGG 

       790        800        810        820        830        840 
VETRPLVRGV GGRSISMKAS PASRSRHGPF KTMPLRWSFG HREKRPGASV ELVEYLESRR 

       850        860        870        880        890        900 
RPRSTSQSIV PLLTRAAGGE ETSASPRSDG TLPAKSEDSG RAIGQGTTGV PLSSCHLNHH 

       910        920        930        940        950        960 
PALGSLDDSL HTARTRTGNV SQDIRLPKKF DLPLTVMPSV GDEKPARPEG QKMTPWKGSS 

       970        980        990       1000       1010       1020 
QVGSQSSPPS PSTGLLRNFK DSGPGTLPKM KSKAAMEERA PDKDRGQGTF TLLKSVFWKK 

      1030       1040       1050       1060       1070       1080 
EHKRTVRTES SPPAPPISLG SDRLSPAAMN EQALRIRESP AKGLGNHMER DIRSAPSSLH 

      1090       1100       1110       1120       1130 
LPRKASRPPR ASTAGTSQRT IPGEQISYGT LQRVKYHTLS LGRKKSLPES SF 

« Hide

Isoform 2 [UniParc].

Checksum: 8EBE4A6ED6AD5AFB
Show »

FASTA1,137124,359

References

[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 21-1132 (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Hippocampus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 207-1132 (ISOFORM 2).
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL732570 Genomic DNA. Translation: CAI52000.2.
AK083271 mRNA. Translation: BAC38837.1. Different initiation.
BC021474 mRNA. Translation: AAH21474.1. Different initiation.
RefSeqNP_776115.2. NM_173754.4.
UniGeneMm.158885.

3D structure databases

ProteinModelPortalQ8BUM9.
SMRQ8BUM9. Positions 99-273, 539-711.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC19.979.

PTM databases

PhosphoSiteQ8BUM9.

Proteomic databases

PRIDEQ8BUM9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021288; ENSMUSP00000021288; ENSMUSG00000020905. [Q8BUM9-1]
GeneID216835.
KEGGmmu:216835.
UCSCuc007jne.1. mouse. [Q8BUM9-1]

Organism-specific databases

CTD124739.
MGIMGI:2444541. Usp43.

Phylogenomic databases

eggNOGCOG5560.
GeneTreeENSGT00750000117419.
HOGENOMHOG000293426.
HOVERGENHBG094126.
InParanoidQ8BUM9.
KOK11856.
OMAWQQQQAH.
OrthoDBEOG7VMP4B.
PhylomeDBQ8BUM9.
TreeFamTF106278.

Gene expression databases

ArrayExpressQ8BUM9.
BgeeQ8BUM9.
CleanExMM_USP43.
GenevestigatorQ8BUM9.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio375380.
PROQ8BUM9.
SOURCESearch...

Entry information

Entry nameUBP43_MOUSE
AccessionPrimary (citable) accession number: Q8BUM9
Secondary accession number(s): Q8VDP5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: April 16, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot