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Q8BUM9

- UBP43_MOUSE

UniProt

Q8BUM9 - UBP43_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase 43

Gene

Usp43

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (19 Sep 2006)
      Previous versions | rss
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    Functioni

    May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei110 – 1101NucleophilePROSITE-ProRule annotation
    Active sitei668 – 6681Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.979.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 43 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 43
    Ubiquitin thioesterase 43
    Ubiquitin-specific-processing protease 43
    Gene namesi
    Name:Usp43
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2444541. Usp43.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11321132Ubiquitin carboxyl-terminal hydrolase 43PRO_0000249522Add
    BLAST

    Proteomic databases

    PRIDEiQ8BUM9.

    PTM databases

    PhosphoSiteiQ8BUM9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BUM9.
    BgeeiQ8BUM9.
    CleanExiMM_USP43.
    GenevestigatoriQ8BUM9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BUM9.
    SMRiQ8BUM9. Positions 99-273, 539-711.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini101 – 710610USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5560.
    GeneTreeiENSGT00750000117419.
    HOGENOMiHOG000293426.
    HOVERGENiHBG094126.
    InParanoidiQ8BUM9.
    KOiK11856.
    OMAiWQQQQAH.
    OrthoDBiEOG7VMP4B.
    PhylomeDBiQ8BUM9.
    TreeFamiTF106278.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BUM9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDPGVGNALG EGPPAPRPRR RRSLRRLLNR FLLALGSRSR SGDSPPRPPA     50
    QPSPYDGDGE GGFACAPGPA PASAGSPGPD RPPGSQPQIS SGDGARPPGA 100
    QGLKNHGNTC FMNAVVQCLS NTDLLAEFLA LGRYRAAPGR AEVTEQLAAL 150
    VRALWTREYT PQLSAEFKNA VSKYGSQFQG NSQHDALEFL LWLLDRVHED 200
    LEGSAHGLVS EQLPPEVSKI SEDLRPSAAP TSLGPSFVQS HFQAQYRSSL 250
    TCPHCLKQSN TFDPFLCVSL PIPLRQTRFL SVTLVFPSKS QRFLRVGLAV 300
    PILSTVAALR KMVAEEGGVP AEEVILVELY PNGFQRSFFD EEDLNTIAEG 350
    DNVYAFQVPP SPGLGTLSAH PSGLSVSPRL PVRDSQRFSG PLHSENRVVF 400
    LFCNLVGSGQ QASRFGPPFL IREDRTISWA QLQQCILSKV RCLMRSEVSA 450
    QDLGTLFSIR VVGLSLACSY LSPKDNRPLC HWAVDRALHL RRPGGPPHVK 500
    LAVEWDSSVT ERLFGSLQEE RVQDADSVWR QQQAHQQPSC TLDECFQSYT 550
    KEEQLAQDDA WKCPHCQVLQ QGVVKLSLWT LPDILIIHLK RFCQVGERRN 600
    KLSTLVKFPL SGLNMAPHVA RRSTNSKAGP GPWSSWKQPI CLPTTYPLDF 650
    LYDLYAVCNH HGSLQGGHYT AYCRNSLDGQ WYSYDDSTVE ALREDEVNTR 700
    GAYILFYQKR NSIPPWSASS SMRGSTSSSL SDHWLMRLGS LNNSTRGSLL 750
    SWSSAPCPSM ARVPDSPVFT NGVCHQDKGG VETRPLVRGV GGRSISMKAS 800
    PASRSRHGPF KTMPLRWSFG HREKRPGASV ELVEYLESRR RPRSTSQSIV 850
    PLLTRAAGGE ETSASPRSDG TLPAKSEDSG RAIGQGTTGV PLSSCHLNHH 900
    PALGSLDDSL HTARTRTGNV SQDIRLPKKF DLPLTVMPSV GDEKPARPEG 950
    QKMTPWKGSS QVGSQSSPPS PSTGLLRNFK DSGPGTLPKM KSKAAMEERA 1000
    PDKDRGQGTF TLLKSVFWKK EHKRTVRTES SPPAPPISLG SDRLSPAAMN 1050
    EQALRIRESP AKGLGNHMER DIRSAPSSLH LPRKASRPPR ASTAGTSQRT 1100
    IPGEQISYGT LQRVKYHTLS LGRKKSLPES SF 1132
    Length:1,132
    Mass (Da):123,874
    Last modified:September 19, 2006 - v2
    Checksum:i3E9E9E46B75E67FA
    GO
    Isoform 2 (identifier: Q8BUM9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1047-1047: Missing.
         1052-1052: Q → QARGSSP

    Note: No experimental confirmation available.

    Show »
    Length:1,137
    Mass (Da):124,359
    Checksum:i8EBE4A6ED6AD5AFB
    GO

    Sequence cautioni

    The sequence AAH21474.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAC38837.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti760 – 7601M → V in AAH21474. (PubMed:15489334)Curated
    Sequence conflicti982 – 9821S → N in AAH21474. (PubMed:15489334)Curated
    Sequence conflicti1002 – 10021D → G in AAH21474. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1047 – 10471Missing in isoform 2. 1 PublicationVSP_020469
    Alternative sequencei1052 – 10521Q → QARGSSP in isoform 2. 1 PublicationVSP_020470

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL732570 Genomic DNA. Translation: CAI52000.2.
    AK083271 mRNA. Translation: BAC38837.1. Different initiation.
    BC021474 mRNA. Translation: AAH21474.1. Different initiation.
    CCDSiCCDS24861.2. [Q8BUM9-1]
    RefSeqiNP_001277978.1. NM_001291049.1.
    NP_776115.2. NM_173754.4. [Q8BUM9-1]
    UniGeneiMm.158885.

    Genome annotation databases

    EnsembliENSMUST00000021288; ENSMUSP00000021288; ENSMUSG00000020905. [Q8BUM9-1]
    GeneIDi216835.
    KEGGimmu:216835.
    UCSCiuc007jne.1. mouse. [Q8BUM9-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL732570 Genomic DNA. Translation: CAI52000.2 .
    AK083271 mRNA. Translation: BAC38837.1 . Different initiation.
    BC021474 mRNA. Translation: AAH21474.1 . Different initiation.
    CCDSi CCDS24861.2. [Q8BUM9-1 ]
    RefSeqi NP_001277978.1. NM_001291049.1.
    NP_776115.2. NM_173754.4. [Q8BUM9-1 ]
    UniGenei Mm.158885.

    3D structure databases

    ProteinModelPortali Q8BUM9.
    SMRi Q8BUM9. Positions 99-273, 539-711.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.979.

    PTM databases

    PhosphoSitei Q8BUM9.

    Proteomic databases

    PRIDEi Q8BUM9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021288 ; ENSMUSP00000021288 ; ENSMUSG00000020905 . [Q8BUM9-1 ]
    GeneIDi 216835.
    KEGGi mmu:216835.
    UCSCi uc007jne.1. mouse. [Q8BUM9-1 ]

    Organism-specific databases

    CTDi 124739.
    MGIi MGI:2444541. Usp43.

    Phylogenomic databases

    eggNOGi COG5560.
    GeneTreei ENSGT00750000117419.
    HOGENOMi HOG000293426.
    HOVERGENi HBG094126.
    InParanoidi Q8BUM9.
    KOi K11856.
    OMAi WQQQQAH.
    OrthoDBi EOG7VMP4B.
    PhylomeDBi Q8BUM9.
    TreeFami TF106278.

    Miscellaneous databases

    NextBioi 375380.
    PROi Q8BUM9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BUM9.
    Bgeei Q8BUM9.
    CleanExi MM_USP43.
    Genevestigatori Q8BUM9.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 21-1132 (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Hippocampus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 207-1132 (ISOFORM 2).
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiUBP43_MOUSE
    AccessioniPrimary (citable) accession number: Q8BUM9
    Secondary accession number(s): Q8VDP5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2006
    Last sequence update: September 19, 2006
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3