Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pleckstrin homology domain-containing family A member 1

Gene

Plekha1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically to phosphatidylinositol 3,4-diphosphate (PtdIns3,4P2), but not to other phosphoinositides. May recruit other proteins to the plasma membrane (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • androgen metabolic process Source: MGI
  • B cell receptor signaling pathway Source: MGI
  • cellular response to hydrogen peroxide Source: MGI
  • establishment of protein localization Source: MGI
  • estrogen metabolic process Source: MGI
  • face morphogenesis Source: MGI
  • female gonad development Source: MGI
  • Leydig cell differentiation Source: MGI
  • luteinization Source: MGI
  • multicellular organism growth Source: MGI
  • negative regulation of protein kinase B signaling Source: MGI
  • palate development Source: MGI
  • phosphatidylinositol 3-kinase signaling Source: MGI
  • platelet-derived growth factor receptor signaling pathway Source: MGI
  • post-embryonic development Source: MGI
  • ruffle organization Source: MGI
  • skeletal system morphogenesis Source: MGI
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family A member 1
Short name:
PH domain-containing family A member 1
Alternative name(s):
Tandem PH domain-containing protein 1
Short name:
TAPP-1
Gene namesi
Name:Plekha1
Synonyms:Tapp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2442213. Plekha1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Nucleus By similarity

  • Note: Locates to the plasma membrane after treatments that stimulate the production of PtdIns3,4P2.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Pleckstrin homology domain-containing family A member 1PRO_0000053874Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Isoform 2 (identifier: Q8BUL6-2)
Modified residuei284 – 2841PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BUL6.
MaxQBiQ8BUL6.
PRIDEiQ8BUL6.

PTM databases

iPTMnetiQ8BUL6.
PhosphoSiteiQ8BUL6.

Expressioni

Gene expression databases

BgeeiQ8BUL6.
ExpressionAtlasiQ8BUL6. baseline and differential.
GenevisibleiQ8BUL6. MM.

Interactioni

Subunit structurei

Interacts with MPDZ and PTPN13.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ8BUL6.
SMRiQ8BUL6. Positions 1-112, 155-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 112106PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini191 – 28999PH 2PROSITE-ProRule annotationAdd
BLAST

Domaini

Binds to membranes enriched in PtdIns3,4P2 via the C-terminal PH domain.

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00440000037398.
HOGENOMiHOG000116175.
HOVERGENiHBG053612.
InParanoidiQ8BUL6.
OMAiNPCIQRS.
PhylomeDBiQ8BUL6.
TreeFamiTF329516.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BUL6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPYVDRQNRI CGFLDIEENE NSGKFLRRYF ILDTREDSFV WYMDNPQNLP
60 70 80 90 100
SGSSRVGAIK LTYISKVSDA TKLRPKAEFC FVMNAGMRKY FLQANDQQDL
110 120 130 140 150
VEWVNVLNKA IKITVPKQSD SQPASDSLSR QGDCGKKQVS YRTDIVGGVP
160 170 180 190 200
IITPTQKEEV NECGESLDRN NLKRSQSHLP YFAPKPPSDS AVIKAGYCVK
210 220 230 240 250
QGAVMKNWKR RYFQLDENTI GYFKSELEKE PLRVIPLKEV HKVQECKQSD
260 270 280 290 300
IMMRDNLFEI VTTSRTFYVQ ADSPEEMHSW IKAVSGAIVA QRGPGRSSSS
310 320 330 340 350
MRQARRLSNP CIQRSIPAGL QNPNSLSVLP GPQPPPHIPQ PLAATLWSQA
360 370 380
VPWRSEEFTN LLPRSSQGTS RSRLSLQESQ LPK
Note: No experimental confirmation available.
Length:383
Mass (Da):43,371
Last modified:March 1, 2003 - v1
Checksum:i6020E5A2F3184C19
GO
Isoform 2 (identifier: Q8BUL6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-115: Missing.
     301-383: MRQARRLSNP...LSLQESQLPK → EHSGWPSEPK...DDASLPVSDV

Note: No experimental confirmation available.Combined sources
Show »
Length:356
Mass (Da):39,575
Checksum:i057CFEA1551371E0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei68 – 11548Missing in isoform 2. 1 PublicationVSP_009764Add
BLAST
Alternative sequencei301 – 38383MRQAR…SQLPK → EHSGWPSEPKFTVRPAGAAA TASHSPASRSHSLVSGCAME KRGVYQSLAKVKPGNFKVQT VSPREPASKVTEQALLRPQS KNGPQGEDGEPVDLDDASLP VSDV in isoform 2. 1 PublicationVSP_009767Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK083340 mRNA. Translation: BAC38874.1.
BC020017 mRNA. Translation: AAH20017.1.
CCDSiCCDS21907.1. [Q8BUL6-2]
RefSeqiNP_598703.1. NM_133942.2. [Q8BUL6-2]
XP_006507211.1. XM_006507148.1. [Q8BUL6-1]
XP_006507212.1. XM_006507149.2. [Q8BUL6-1]
UniGeneiMm.323554.

Genome annotation databases

EnsembliENSMUST00000048180; ENSMUSP00000035375; ENSMUSG00000040268. [Q8BUL6-2]
ENSMUST00000075181; ENSMUSP00000074675; ENSMUSG00000040268. [Q8BUL6-1]
GeneIDi101476.
KEGGimmu:101476.
UCSCiuc009kar.1. mouse. [Q8BUL6-1]
uc009kat.1. mouse. [Q8BUL6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK083340 mRNA. Translation: BAC38874.1.
BC020017 mRNA. Translation: AAH20017.1.
CCDSiCCDS21907.1. [Q8BUL6-2]
RefSeqiNP_598703.1. NM_133942.2. [Q8BUL6-2]
XP_006507211.1. XM_006507148.1. [Q8BUL6-1]
XP_006507212.1. XM_006507149.2. [Q8BUL6-1]
UniGeneiMm.323554.

3D structure databases

ProteinModelPortaliQ8BUL6.
SMRiQ8BUL6. Positions 1-112, 155-305.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ8BUL6.
PhosphoSiteiQ8BUL6.

Proteomic databases

EPDiQ8BUL6.
MaxQBiQ8BUL6.
PRIDEiQ8BUL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048180; ENSMUSP00000035375; ENSMUSG00000040268. [Q8BUL6-2]
ENSMUST00000075181; ENSMUSP00000074675; ENSMUSG00000040268. [Q8BUL6-1]
GeneIDi101476.
KEGGimmu:101476.
UCSCiuc009kar.1. mouse. [Q8BUL6-1]
uc009kat.1. mouse. [Q8BUL6-2]

Organism-specific databases

CTDi59338.
MGIiMGI:2442213. Plekha1.

Phylogenomic databases

GeneTreeiENSGT00440000037398.
HOGENOMiHOG000116175.
HOVERGENiHBG053612.
InParanoidiQ8BUL6.
OMAiNPCIQRS.
PhylomeDBiQ8BUL6.
TreeFamiTF329516.

Miscellaneous databases

PROiQ8BUL6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BUL6.
ExpressionAtlasiQ8BUL6. baseline and differential.
GenevisibleiQ8BUL6. MM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: Czech II.
    Tissue: Mammary gland.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Lung.

Entry informationi

Entry nameiPKHA1_MOUSE
AccessioniPrimary (citable) accession number: Q8BUL6
Secondary accession number(s): Q8BK96, Q8BXU1, Q8VE13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.