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Protein

Protein Hook homolog 3

Gene

Hook3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex (By similarity). Serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking.By similarity

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic microtubule organization Source: MGI
  • early endosome to late endosome transport Source: UniProtKB
  • endosome organization Source: UniProtKB
  • endosome to lysosome transport Source: UniProtKB
  • Golgi localization Source: MGI
  • interkinetic nuclear migration Source: BHF-UCL
  • lysosome organization Source: UniProtKB
  • microtubule anchoring at centrosome Source: MGI
  • negative regulation of neurogenesis Source: BHF-UCL
  • neuronal stem cell population maintenance Source: MGI
  • protein localization to centrosome Source: BHF-UCL
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Hook homolog 3
Short name:
mHK3
Gene namesi
Name:Hook3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2443554. Hook3.

Subcellular locationi

  • Cytoplasmcytoskeleton 1 Publication
  • Golgi apparatus 1 Publication

  • Note: Enriched at the cis-face of the Golgi complex. Localizes to microtubule asters in prophase.By similarity

GO - Cellular componenti

  • centriolar satellite Source: MGI
  • centrosome Source: BHF-UCL
  • cis-Golgi network Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: GOC
  • FHF complex Source: MGI
  • Golgi apparatus Source: MGI
  • HOPS complex Source: Ensembl
  • microtubule Source: UniProtKB-KW
  • microtubule organizing center Source: MGI
  • pericentriolar material Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718Protein Hook homolog 3PRO_0000219198Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei238 – 2381PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BUK6.
MaxQBiQ8BUK6.
PaxDbiQ8BUK6.
PRIDEiQ8BUK6.

PTM databases

iPTMnetiQ8BUK6.
PhosphoSiteiQ8BUK6.

Expressioni

Tissue specificityi

Expressed in brain, cerebellum, heart, intestine, kidney, liver, lung, skeletal muscle, spleen and stomach (at protein level).2 Publications

Gene expression databases

BgeeiQ8BUK6.
CleanExiMM_HOOK3.
ExpressionAtlasiQ8BUK6. baseline and differential.
GenevisibleiQ8BUK6. MM.

Interactioni

Subunit structurei

Self-associates. Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3. May interact directly with AKTIP/FTS, HOOK1 and HOOK2. Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect. Interacts with microtubules (By similarity). Interacts with MSR1, and this association is stimulated by ligand binding to MSR1. Interacts with IIGP1. Interacts with Salmonella typhimurium spiC.By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi235828. 1 interaction.
IntActiQ8BUK6. 3 interactions.
MINTiMINT-4097815.
STRINGi10090.ENSMUSP00000046788.

Structurei

3D structure databases

ProteinModelPortaliQ8BUK6.
SMRiQ8BUK6. Positions 12-166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 164164Sufficient for interaction with microtubulesBy similarityAdd
BLAST
Regioni450 – 671222Sufficient for interaction with IIGP1Add
BLAST
Regioni553 – 718166Required for association with GolgiBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili168 – 433266Sequence analysisAdd
BLAST
Coiled coili462 – 663202Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the hook family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG1M. Eukaryota.
ENOG410XQ3G. LUCA.
GeneTreeiENSGT00690000101702.
HOVERGENiHBG051920.
InParanoidiQ8BUK6.
KOiK16536.
OMAiGVEAPCK.
OrthoDBiEOG7ZD1V5.
PhylomeDBiQ8BUK6.
TreeFamiTF320231.

Family and domain databases

InterProiIPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 1 hit.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BUK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNVESVERV ELCESLLTWI QTFNVDAPCQ TAEDLTNGVV MSQVLQKIDP
60 70 80 90 100
VYFDDNWLNR IKTEVGDNWR LKISNLKKIL KGILDYNHEI LGQQINDFTL
110 120 130 140 150
PDVNLIGEHS DAAELGRMLQ LILGCAVNCE QKQEYIQAIM MMEESVQHVV
160 170 180 190 200
MTAIQELMSK ESPVSAGHDA YVDLDRQLKK TTEELNEALS AKEEIAQRCH
210 220 230 240 250
ELDMQVAALQ EEKSSLLAEN QILMERLNQS DSIEDPNSPA GRRHLQLQTQ
260 270 280 290 300
LEQLQEETFR LEAAKDDYRI RCEELEKEIS ELRQQNDELT TLADEAQSLK
310 320 330 340 350
DEIDVLRHSS DKVSKLEGQV ESYKKKLEDL GDLRRQVKLL EEKNTMYMQN
360 370 380 390 400
TVSLEEELRK ANAARGQLET YKRQVVELQN RLSDESKKAD KLDFEYKRLK
410 420 430 440 450
EKVDGLQKEK DRLRTERDSL KETIEELRCV QAQEGQLTTQ GLMPLGSQES
460 470 480 490 500
SDSLAAEIVT PEIREKLIRL QHENKMLKLN QEDSDNEKIA LLQSLLDDAN
510 520 530 540 550
LRKNELETEN RLVNQRLLEV QSQVEELQKS LQDQGSKAED SVLLKKKLEE
560 570 580 590 600
HLEKLHEANN ELQKKRAIIE DLEPRFNNSS LRIEELQEAL RKKEEEMKQM
610 620 630 640 650
EERYKKYLEK AKSVIRTLDP KQNQGAAPEI QALKNQLQER DRLFHSLEKE
660 670 680 690 700
YEKTKSQRDM EEKYIVSAWY NMGMTLHKKA AEDRLASTGS GQSFLARQRQ
710
ATSTRRSYPG HVQPATAR
Length:718
Mass (Da):83,218
Last modified:February 2, 2004 - v2
Checksum:i72577D065F118172
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti94 – 941Q → H in BAC37897 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY223806 mRNA. Translation: AAO43109.1.
AK042180 mRNA. Translation: BAC31191.1.
AK054263 mRNA. Translation: BAC35709.1.
AK080377 mRNA. Translation: BAC37897.1.
AK084594 mRNA. Translation: BAC39222.1.
AK145149 mRNA. Translation: BAE26261.1.
CH466580 Genomic DNA. Translation: EDL32811.1.
CCDSiCCDS22206.1.
RefSeqiNP_997542.1. NM_207659.3.
UniGeneiMm.334464.
Mm.458186.

Genome annotation databases

EnsembliENSMUST00000037182; ENSMUSP00000046788; ENSMUSG00000037234.
GeneIDi320191.
KEGGimmu:320191.
UCSCiuc009lhj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY223806 mRNA. Translation: AAO43109.1.
AK042180 mRNA. Translation: BAC31191.1.
AK054263 mRNA. Translation: BAC35709.1.
AK080377 mRNA. Translation: BAC37897.1.
AK084594 mRNA. Translation: BAC39222.1.
AK145149 mRNA. Translation: BAE26261.1.
CH466580 Genomic DNA. Translation: EDL32811.1.
CCDSiCCDS22206.1.
RefSeqiNP_997542.1. NM_207659.3.
UniGeneiMm.334464.
Mm.458186.

3D structure databases

ProteinModelPortaliQ8BUK6.
SMRiQ8BUK6. Positions 12-166.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi235828. 1 interaction.
IntActiQ8BUK6. 3 interactions.
MINTiMINT-4097815.
STRINGi10090.ENSMUSP00000046788.

PTM databases

iPTMnetiQ8BUK6.
PhosphoSiteiQ8BUK6.

Proteomic databases

EPDiQ8BUK6.
MaxQBiQ8BUK6.
PaxDbiQ8BUK6.
PRIDEiQ8BUK6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037182; ENSMUSP00000046788; ENSMUSG00000037234.
GeneIDi320191.
KEGGimmu:320191.
UCSCiuc009lhj.2. mouse.

Organism-specific databases

CTDi84376.
MGIiMGI:2443554. Hook3.

Phylogenomic databases

eggNOGiENOG410IG1M. Eukaryota.
ENOG410XQ3G. LUCA.
GeneTreeiENSGT00690000101702.
HOVERGENiHBG051920.
InParanoidiQ8BUK6.
KOiK16536.
OMAiGVEAPCK.
OrthoDBiEOG7ZD1V5.
PhylomeDBiQ8BUK6.
TreeFamiTF320231.

Miscellaneous databases

ChiTaRSiHook3. mouse.
PROiQ8BUK6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BUK6.
CleanExiMM_HOOK3.
ExpressionAtlasiQ8BUK6. baseline and differential.
GenevisibleiQ8BUK6. MM.

Family and domain databases

InterProiIPR008636. Hook-related_fam.
[Graphical view]
PANTHERiPTHR18947. PTHR18947. 1 hit.
PfamiPF05622. HOOK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "IIGP, a member of the IFN inducible and microbial defense mediating 47 kDa GTPase family, interacts with the microtubule binding protein hook3."
    Kaiser F., Kaufmann S.H.E., Zerrahn J.
    J. Cell Sci. 117:1747-1756(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], INTERACTION WITH IIGP1, SUBCELLULAR LOCATION.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart, Mammary gland, Ovary and Thymus.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The Golgi-associated hook3 protein is a member of a novel family of microtubule-binding proteins."
    Walenta J.H., Didier A.J., Liu X., Kraemer H.
    J. Cell Biol. 152:923-934(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "The Salmonella SpiC protein targets the mammalian Hook3 protein function to alter cellular trafficking."
    Shotland Y., Kraemer H., Groisman E.A.
    Mol. Microbiol. 49:1565-1576(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPIC.
  6. "The microtubule-binding protein Hook3 interacts with a cytoplasmic domain of scavenger receptor A."
    Sano H., Ishino M., Kraemer H., Shimizu T., Mitsuzawa H., Nishitani C., Kuroki Y.
    J. Biol. Chem. 282:7973-7981(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MSR1.
  7. "Hook2 localizes to the centrosome, binds directly to centriolin/CEP110 and contributes to centrosomal function."
    Szebenyi G., Hall B., Yu R., Hashim A.I., Kraemer H.
    Traffic 8:32-46(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-238, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiHOOK3_MOUSE
AccessioniPrimary (citable) accession number: Q8BUK6
Secondary accession number(s): Q540A0
, Q8BV48, Q8BW57, Q8BY41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: June 8, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.