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Protein

Protein Hook homolog 3

Gene

Hook3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex (By similarity). Serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (PubMed:12950921). Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • cytoplasmic microtubule organization Source: MGI
  • cytoskeleton-dependent intracellular transport Source: GO_Central
  • early endosome to late endosome transport Source: UniProtKB
  • endosome organization Source: UniProtKB
  • endosome to lysosome transport Source: UniProtKB
  • Golgi localization Source: MGI
  • interkinetic nuclear migration Source: BHF-UCL
  • lysosome organization Source: UniProtKB
  • microtubule anchoring at centrosome Source: MGI
  • negative regulation of neurogenesis Source: BHF-UCL
  • neuronal stem cell population maintenance Source: MGI
  • protein localization to centrosome Source: BHF-UCL
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Hook homolog 3
Short name:
mHK3
Gene namesi
Name:Hook3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2443554 Hook3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191981 – 718Protein Hook homolog 3Add BLAST718

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei238PhosphoserineCombined sources1
Modified residuei693PhosphoserineBy similarity1
Modified residuei707PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BUK6
MaxQBiQ8BUK6
PaxDbiQ8BUK6
PeptideAtlasiQ8BUK6
PRIDEiQ8BUK6

PTM databases

iPTMnetiQ8BUK6
PhosphoSitePlusiQ8BUK6

Expressioni

Tissue specificityi

Expressed in brain, cerebellum, heart, intestine, kidney, liver, lung, skeletal muscle, spleen and stomach (at protein level).2 Publications

Gene expression databases

BgeeiENSMUSG00000037234
CleanExiMM_HOOK3
ExpressionAtlasiQ8BUK6 baseline and differential
GenevisibleiQ8BUK6 MM

Interactioni

Subunit structurei

Self-associates (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex), composed of AKTIP/FTS, FAM160A2, and one or more members of the Hook family of proteins HOOK1, HOOK2, and HOOK3 (By similarity). May interact directly with AKTIP/FTS, HOOK1 and HOOK2 (By similarity). Associates with several subunits of the homotypic vesicular sorting complex (the HOPS complex) including VPS16 and VPS41; these interactions may be indirect (By similarity). Interacts with MSR1, and this association is stimulated by ligand binding to MSR1 (PubMed:17237231). Interacts with microtubules (By similarity). Interacts with IIGP1 (PubMed:15075236). Interacts with Salmonella typhimurium spiC (PubMed:12950921). Interacts with dynein intermediate chain and dynactin (DCTN1) (By similarity). Interacts with CCDC181 (PubMed:28283191). Interacts with LRGUK (PubMed:28003339).By similarity5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi235828, 3 interactors
IntActiQ8BUK6, 5 interactors
MINTiQ8BUK6
STRINGi10090.ENSMUSP00000046788

Structurei

3D structure databases

ProteinModelPortaliQ8BUK6
SMRiQ8BUK6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 126Calponin-homology (CH)PROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 164Sufficient for interaction with microtubulesBy similarityAdd BLAST164
Regioni450 – 671Sufficient for interaction with IIGP11 PublicationAdd BLAST222
Regioni553 – 718Required for association with GolgiBy similarityAdd BLAST166

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili168 – 433Sequence analysisAdd BLAST266
Coiled coili462 – 663Sequence analysisAdd BLAST202

Sequence similaritiesi

Belongs to the hook family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG1M Eukaryota
ENOG410XQ3G LUCA
GeneTreeiENSGT00690000101702
HOVERGENiHBG051920
InParanoidiQ8BUK6
KOiK16536
OMAiDELKCGQ
OrthoDBiEOG091G06O9
PhylomeDBiQ8BUK6
TreeFamiTF320231

Family and domain databases

Gene3Di1.10.418.10, 1 hit
InterProiView protein in InterPro
IPR001715 CH-domain
IPR036872 CH_dom_sf
IPR008636 Hook-like_fam
PANTHERiPTHR18947 PTHR18947, 1 hit
PfamiView protein in Pfam
PF05622 HOOK, 1 hit
PROSITEiView protein in PROSITE
PS50021 CH, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BUK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNVESVERV ELCESLLTWI QTFNVDAPCQ TAEDLTNGVV MSQVLQKIDP
60 70 80 90 100
VYFDDNWLNR IKTEVGDNWR LKISNLKKIL KGILDYNHEI LGQQINDFTL
110 120 130 140 150
PDVNLIGEHS DAAELGRMLQ LILGCAVNCE QKQEYIQAIM MMEESVQHVV
160 170 180 190 200
MTAIQELMSK ESPVSAGHDA YVDLDRQLKK TTEELNEALS AKEEIAQRCH
210 220 230 240 250
ELDMQVAALQ EEKSSLLAEN QILMERLNQS DSIEDPNSPA GRRHLQLQTQ
260 270 280 290 300
LEQLQEETFR LEAAKDDYRI RCEELEKEIS ELRQQNDELT TLADEAQSLK
310 320 330 340 350
DEIDVLRHSS DKVSKLEGQV ESYKKKLEDL GDLRRQVKLL EEKNTMYMQN
360 370 380 390 400
TVSLEEELRK ANAARGQLET YKRQVVELQN RLSDESKKAD KLDFEYKRLK
410 420 430 440 450
EKVDGLQKEK DRLRTERDSL KETIEELRCV QAQEGQLTTQ GLMPLGSQES
460 470 480 490 500
SDSLAAEIVT PEIREKLIRL QHENKMLKLN QEDSDNEKIA LLQSLLDDAN
510 520 530 540 550
LRKNELETEN RLVNQRLLEV QSQVEELQKS LQDQGSKAED SVLLKKKLEE
560 570 580 590 600
HLEKLHEANN ELQKKRAIIE DLEPRFNNSS LRIEELQEAL RKKEEEMKQM
610 620 630 640 650
EERYKKYLEK AKSVIRTLDP KQNQGAAPEI QALKNQLQER DRLFHSLEKE
660 670 680 690 700
YEKTKSQRDM EEKYIVSAWY NMGMTLHKKA AEDRLASTGS GQSFLARQRQ
710
ATSTRRSYPG HVQPATAR
Length:718
Mass (Da):83,218
Last modified:February 2, 2004 - v2
Checksum:i72577D065F118172
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94Q → H in BAC37897 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY223806 mRNA Translation: AAO43109.1
AK042180 mRNA Translation: BAC31191.1
AK054263 mRNA Translation: BAC35709.1
AK080377 mRNA Translation: BAC37897.1
AK084594 mRNA Translation: BAC39222.1
AK145149 mRNA Translation: BAE26261.1
CH466580 Genomic DNA Translation: EDL32811.1
CCDSiCCDS22206.1
RefSeqiNP_997542.1, NM_207659.3
UniGeneiMm.334464
Mm.458186

Genome annotation databases

EnsembliENSMUST00000037182; ENSMUSP00000046788; ENSMUSG00000037234
GeneIDi320191
KEGGimmu:320191
UCSCiuc009lhj.2 mouse

Similar proteinsi

Entry informationi

Entry nameiHOOK3_MOUSE
AccessioniPrimary (citable) accession number: Q8BUK6
Secondary accession number(s): Q540A0
, Q8BV48, Q8BW57, Q8BY41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: May 23, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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