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Protein

Apoptosis-inducing factor 2

Gene

Aifm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death (By similarity). May contribute to genotoxin-induced growth arrest.By similarity

Cofactori

6-hydroxy-FADBy similarityNote: Binds 6-hydroxy-FAD non-covalently.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 5416-hydroxy-FADSequence analysis
Binding sitei82 – 8216-hydroxy-FAD; via amide nitrogen and carbonyl oxygenSequence analysis
Binding sitei285 – 28516-hydroxy-FADSequence analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 2256-hydroxy-FADSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

DNA-binding, FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis-inducing factor 2 (EC:1.-.-.-)
Alternative name(s):
Apoptosis-inducing factor homologous mitochondrion-associated inducer of death
Apoptosis-inducing factor-like mitochondrion-associated inducer of death
Gene namesi
Name:Aifm2
Synonyms:Amid
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1918611. Aifm2.

Subcellular locationi

  • Cytoplasm
  • Mitochondrion outer membrane By similarity
  • Membrane By similarity; Lipid-anchor By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 373372Apoptosis-inducing factor 2PRO_0000238923Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiQ8BUE4.
PaxDbiQ8BUE4.
PRIDEiQ8BUE4.

PTM databases

iPTMnetiQ8BUE4.
PhosphoSiteiQ8BUE4.

Expressioni

Tissue specificityi

Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in liver, testis, and kidney, and expressed at lower levels in pancreas, spleen, brain and lung.1 Publication

Inductioni

Expression is up-regulated in mouse embryonic fibroblasts by genotoxic reagents 5-fluorouracil and etoposide.1 Publication

Gene expression databases

BgeeiQ8BUE4.
GenevisibleiQ8BUE4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078998.

Structurei

3D structure databases

ProteinModelPortaliQ8BUE4.
SMRiQ8BUE4. Positions 12-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQ9K. Eukaryota.
COG1252. LUCA.
GeneTreeiENSGT00390000004582.
HOGENOMiHOG000238788.
HOVERGENiHBG054912.
InParanoidiQ8BUE4.
OMAiKINNSAY.
OrthoDBiEOG7J447T.
PhylomeDBiQ8BUE4.
TreeFamiTF329369.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PRINTSiPR00469. PNDRDTASEII.
SUPFAMiSSF51905. SSF51905. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q8BUE4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSQVSVDTG AVHVVIVGGG FGGIAAASQL QALNVPFMLV DMKDSFHHNV
60 70 80 90 100
AALRASVESG FAKKTFISYS ATFKDNFRQG KVIGIDLKNR MVLLQGGEAL
110 120 130 140 150
PFSHLILATG STGPFPGKFN EVSCQQAAIQ AYEDMVKQIQ RSQFIVVVGG
160 170 180 190 200
GSAGVEMAAE IKTEYPEKEV TLIHSRVPLA DKELLPCVRQ EVKEILLRKG
210 220 230 240 250
VQLLLSERVS NLEELPRNEY REYIKVETDK GTEVATNMVI VCNGIKINSS
260 270 280 290 300
AYRSAFESRL ASNGALKVNE FLQVEGYSNI YAIGDCADTK EPKMAYHAGL
310 320 330 340 350
HANVAVANIV NSMKQRPLKA YKPGALTFLL SMGRNDGVGQ ISGFYVGRLM
360 370
VRLAKSRDLL ISTSWKTMRQ SPP
Note: No experimental confirmation available.Curated
Length:373
Mass (Da):40,635
Last modified:March 1, 2003 - v1
Checksum:iA85F02A26C1084BA
GO
Isoform 21 Publication (identifier: Q8BUE4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     324-373: GALTFLLSMG...SWKTMRQSPP → ETDQPPAALS...FSDRPCVISL

Note: No experimental confirmation available.Curated
Show »
Length:380
Mass (Da):41,356
Checksum:i65BC3D1273959D5E
GO

Sequence cautioni

Isoform 2 : The sequence AAH38129.1 differs from that shown.erroneous termination (Translated as Trp in position: 339:Stop->W)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei324 – 37350GALTF…RQSPP → ETDQPPAALSPALLLWTPAR KLTLSEGRINYLQEAGHVRT LQSMAGLFSDRPCVISL in isoform 2. 1 PublicationVSP_052049Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085656 mRNA. Translation: BAC39497.1.
AK155240 mRNA. Translation: BAE33142.1.
AK147741 mRNA. Translation: BAE28108.1.
AC153136 Genomic DNA. No translation available.
BC038129 mRNA. Translation: AAH38129.1. Sequence problems.
CCDSiCCDS23884.1. [Q8BUE4-1]
CCDS48576.1. [Q8BUE4-2]
RefSeqiNP_001034283.1. NM_001039194.3. [Q8BUE4-1]
NP_001271229.1. NM_001284300.1. [Q8BUE4-1]
NP_722474.2. NM_153779.2. [Q8BUE4-2]
NP_835159.1. NM_178058.4. [Q8BUE4-1]
XP_006514187.1. XM_006514124.2. [Q8BUE4-1]
XP_006514191.1. XM_006514128.2. [Q8BUE4-2]
UniGeneiMm.286309.

Genome annotation databases

EnsembliENSMUST00000067857; ENSMUSP00000070054; ENSMUSG00000020085. [Q8BUE4-1]
ENSMUST00000080099; ENSMUSP00000078998; ENSMUSG00000020085. [Q8BUE4-2]
ENSMUST00000099706; ENSMUSP00000097297; ENSMUSG00000020085. [Q8BUE4-1]
ENSMUST00000105455; ENSMUSP00000101095; ENSMUSG00000020085. [Q8BUE4-1]
GeneIDi71361.
KEGGimmu:71361.
UCSCiuc007fgi.2. mouse. [Q8BUE4-1]
uc007fgl.1. mouse. [Q8BUE4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085656 mRNA. Translation: BAC39497.1.
AK155240 mRNA. Translation: BAE33142.1.
AK147741 mRNA. Translation: BAE28108.1.
AC153136 Genomic DNA. No translation available.
BC038129 mRNA. Translation: AAH38129.1. Sequence problems.
CCDSiCCDS23884.1. [Q8BUE4-1]
CCDS48576.1. [Q8BUE4-2]
RefSeqiNP_001034283.1. NM_001039194.3. [Q8BUE4-1]
NP_001271229.1. NM_001284300.1. [Q8BUE4-1]
NP_722474.2. NM_153779.2. [Q8BUE4-2]
NP_835159.1. NM_178058.4. [Q8BUE4-1]
XP_006514187.1. XM_006514124.2. [Q8BUE4-1]
XP_006514191.1. XM_006514128.2. [Q8BUE4-2]
UniGeneiMm.286309.

3D structure databases

ProteinModelPortaliQ8BUE4.
SMRiQ8BUE4. Positions 12-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078998.

PTM databases

iPTMnetiQ8BUE4.
PhosphoSiteiQ8BUE4.

Proteomic databases

MaxQBiQ8BUE4.
PaxDbiQ8BUE4.
PRIDEiQ8BUE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067857; ENSMUSP00000070054; ENSMUSG00000020085. [Q8BUE4-1]
ENSMUST00000080099; ENSMUSP00000078998; ENSMUSG00000020085. [Q8BUE4-2]
ENSMUST00000099706; ENSMUSP00000097297; ENSMUSG00000020085. [Q8BUE4-1]
ENSMUST00000105455; ENSMUSP00000101095; ENSMUSG00000020085. [Q8BUE4-1]
GeneIDi71361.
KEGGimmu:71361.
UCSCiuc007fgi.2. mouse. [Q8BUE4-1]
uc007fgl.1. mouse. [Q8BUE4-2]

Organism-specific databases

CTDi84883.
MGIiMGI:1918611. Aifm2.

Phylogenomic databases

eggNOGiENOG410IQ9K. Eukaryota.
COG1252. LUCA.
GeneTreeiENSGT00390000004582.
HOGENOMiHOG000238788.
HOVERGENiHBG054912.
InParanoidiQ8BUE4.
OMAiKINNSAY.
OrthoDBiEOG7J447T.
PhylomeDBiQ8BUE4.
TreeFamiTF329369.

Miscellaneous databases

NextBioi333621.
PROiQ8BUE4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BUE4.
GenevisibleiQ8BUE4. MM.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR000103. Pyridine_nuc-diS_OxRdtase_2.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
PRINTSiPR00469. PNDRDTASEII.
SUPFAMiSSF51905. SSF51905. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6JImported and NODImported.
    Tissue: Dendritic cellImported, Mammary glandImported and MelanocyteImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6JImported.
    Tissue: Mammary glandImported.
  4. "The p53-inducible apoptotic protein AMID is not required for normal development and tumor suppression."
    Mei J., Webb S., Zhang B., Shu H.-B.
    Oncogene 25:849-856(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Lung and Spleen.

Entry informationi

Entry nameiAIFM2_MOUSE
AccessioniPrimary (citable) accession number: Q8BUE4
Secondary accession number(s): Q8CHZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 1, 2003
Last modified: January 20, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.