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Protein

Apoptosis-inducing factor 2

Gene

Aifm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Oxidoreductase, which may play a role in mediating a p53/TP53-dependent apoptosis response. Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death (By similarity). May contribute to genotoxin-induced growth arrest.By similarity

Cofactori

6-hydroxy-FADBy similarityNote: Binds 6-hydroxy-FAD non-covalently.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei546-hydroxy-FADSequence analysis1
Binding sitei826-hydroxy-FAD; via amide nitrogen and carbonyl oxygenSequence analysis1
Binding sitei2856-hydroxy-FADSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 226-hydroxy-FADSequence analysis5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Oxidoreductase
LigandFAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis-inducing factor 2 (EC:1.-.-.-)
Alternative name(s):
Apoptosis-inducing factor homologous mitochondrion-associated inducer of death
Apoptosis-inducing factor-like mitochondrion-associated inducer of death
Gene namesi
Name:Aifm2
Synonyms:Amid
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1918611 Aifm2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002389232 – 373Apoptosis-inducing factor 2Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiQ8BUE4
PeptideAtlasiQ8BUE4
PRIDEiQ8BUE4

PTM databases

iPTMnetiQ8BUE4
PhosphoSitePlusiQ8BUE4

Expressioni

Tissue specificityi

Detected in most normal tissues as two transcripts of 1.8 and 4.0 kb in length, respectively. Highly expressed in liver, testis, and kidney, and expressed at lower levels in pancreas, spleen, brain and lung.1 Publication

Inductioni

Expression is up-regulated in mouse embryonic fibroblasts by genotoxic reagents 5-fluorouracil and etoposide.1 Publication

Gene expression databases

BgeeiENSMUSG00000020085
GenevisibleiQ8BUE4 MM

Interactioni

Protein-protein interaction databases

BioGridi214662, 1 interactor
IntActiQ8BUE4, 1 interactor
STRINGi10090.ENSMUSP00000078998

Structurei

3D structure databases

ProteinModelPortaliQ8BUE4
SMRiQ8BUE4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQ9K Eukaryota
COG1252 LUCA
GeneTreeiENSGT00390000004582
HOGENOMiHOG000238788
HOVERGENiHBG054912
InParanoidiQ8BUE4
OMAiGDCADVR
OrthoDBiEOG091G0AED
PhylomeDBiQ8BUE4
TreeFamiTF329369

Family and domain databases

InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR000103 Pyridine_nuc-diS_OxRdtase_2
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PRINTSiPR00469 PNDRDTASEII
SUPFAMiSSF51905 SSF51905, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q8BUE4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSQVSVDTG AVHVVIVGGG FGGIAAASQL QALNVPFMLV DMKDSFHHNV
60 70 80 90 100
AALRASVESG FAKKTFISYS ATFKDNFRQG KVIGIDLKNR MVLLQGGEAL
110 120 130 140 150
PFSHLILATG STGPFPGKFN EVSCQQAAIQ AYEDMVKQIQ RSQFIVVVGG
160 170 180 190 200
GSAGVEMAAE IKTEYPEKEV TLIHSRVPLA DKELLPCVRQ EVKEILLRKG
210 220 230 240 250
VQLLLSERVS NLEELPRNEY REYIKVETDK GTEVATNMVI VCNGIKINSS
260 270 280 290 300
AYRSAFESRL ASNGALKVNE FLQVEGYSNI YAIGDCADTK EPKMAYHAGL
310 320 330 340 350
HANVAVANIV NSMKQRPLKA YKPGALTFLL SMGRNDGVGQ ISGFYVGRLM
360 370
VRLAKSRDLL ISTSWKTMRQ SPP
Note: No experimental confirmation available.Curated
Length:373
Mass (Da):40,635
Last modified:March 1, 2003 - v1
Checksum:iA85F02A26C1084BA
GO
Isoform 21 Publication (identifier: Q8BUE4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     324-373: GALTFLLSMG...SWKTMRQSPP → ETDQPPAALS...FSDRPCVISL

Note: No experimental confirmation available.Curated
Show »
Length:380
Mass (Da):41,356
Checksum:i65BC3D1273959D5E
GO

Sequence cautioni

Isoform 2 : The sequence AAH38129 differs from that shown. erroneous termination (Translated as Trp in position: 339:Stop->W)Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052049324 – 373GALTF…RQSPP → ETDQPPAALSPALLLWTPAR KLTLSEGRINYLQEAGHVRT LQSMAGLFSDRPCVISL in isoform 2. 1 PublicationAdd BLAST50

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK085656 mRNA Translation: BAC39497.1
AK155240 mRNA Translation: BAE33142.1
AK147741 mRNA Translation: BAE28108.1
AC153136 Genomic DNA No translation available.
BC038129 mRNA Translation: AAH38129.1 Sequence problems.
CCDSiCCDS23884.1 [Q8BUE4-1]
CCDS48576.1 [Q8BUE4-2]
RefSeqiNP_001034283.1, NM_001039194.3 [Q8BUE4-1]
NP_001271229.1, NM_001284300.1 [Q8BUE4-1]
NP_722474.2, NM_153779.2 [Q8BUE4-2]
NP_835159.1, NM_178058.4 [Q8BUE4-1]
XP_006514187.1, XM_006514124.3 [Q8BUE4-1]
UniGeneiMm.286309

Genome annotation databases

EnsembliENSMUST00000067857; ENSMUSP00000070054; ENSMUSG00000020085 [Q8BUE4-1]
ENSMUST00000080099; ENSMUSP00000078998; ENSMUSG00000020085 [Q8BUE4-2]
ENSMUST00000099706; ENSMUSP00000097297; ENSMUSG00000020085 [Q8BUE4-1]
ENSMUST00000105455; ENSMUSP00000101095; ENSMUSG00000020085 [Q8BUE4-1]
GeneIDi71361
KEGGimmu:71361
UCSCiuc007fgi.2 mouse [Q8BUE4-1]
uc007fgl.1 mouse [Q8BUE4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAIFM2_MOUSE
AccessioniPrimary (citable) accession number: Q8BUE4
Secondary accession number(s): Q8CHZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health