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Protein

WD repeat and FYVE domain-containing protein 2

Gene

Wdfy2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts in an adapter protein-like fashion to mediate the interaction between the kinase PRKCZ and its substrate VAMP2 and increases the PRKCZ-dependent phosphorylation of VAMP2 (By similarity). Positively regulates adipocyte differentiation, by facilitating the phosphorylation and thus inactivation of the anti-adipogenetic transcription factor FOXO1 by the kinase AKT1 (PubMed:18388859). Plays a role in endosomal control of AKT2 signaling; required for insulin-stimulated AKT2 phosphorylation and glucose uptake and insulin-stimulated phosphorylation of AKT2 substrates (PubMed:20189988). Participates in transferrin receptor endocytosis (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri281 – 352FYVE-typePROSITE-ProRule annotationAdd BLAST72

GO - Molecular functioni

GO - Biological processi

  • positive regulation of fat cell differentiation Source: UniProtKB
  • positive regulation of protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat and FYVE domain-containing protein 2
Alternative name(s):
Propeller-FYVE protein1 Publication
Short name:
Prof
WD40- and FYVE domain-containing protein 2
Gene namesi
Name:Wdfy2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2442811. Wdfy2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • early endosome Source: UniProtKB
  • vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000513371 – 400WD repeat and FYVE domain-containing protein 2Add BLAST400

Proteomic databases

MaxQBiQ8BUB4.
PaxDbiQ8BUB4.
PeptideAtlasiQ8BUB4.
PRIDEiQ8BUB4.

PTM databases

PhosphoSitePlusiQ8BUB4.

Expressioni

Tissue specificityi

Highly expressed in the brain (at protein level).1 Publication

Inductioni

Transiently up-regulated during adipogenesis (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000014547.
CleanExiMM_WDFY2.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts (via WD repeats 1-3) with AKT1, AKT2, PRKCZ and PRKCI (PubMed:16792529, PubMed:20189988). Interacts with VAMP2 (PubMed:17313651). Forms a complex with VAMP2 and PRKCZ (PubMed:17313651). Interacts with FOXO1 (PubMed:18388859). Forms a complex with AKT1 and FOXO1 (PubMed:18388859).By similarity4 Publications

Protein-protein interaction databases

BioGridi234549. 1 interactor.
IntActiQ8BUB4. 2 interactors.
STRINGi10090.ENSMUSP00000014691.

Structurei

3D structure databases

ProteinModelPortaliQ8BUB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati22 – 61WD 1Add BLAST40
Repeati66 – 105WD 2Add BLAST40
Repeati112 – 150WD 3Add BLAST39
Repeati153 – 192WD 4Add BLAST40
Repeati197 – 236WD 5Add BLAST40
Repeati240 – 279WD 6Add BLAST40
Repeati364 – 399WD 7Add BLAST36

Domaini

The FYVE-type zinc finger is essential for its vesicular localization.By similarity

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri281 – 352FYVE-typePROSITE-ProRule annotationAdd BLAST72

Keywords - Domaini

Repeat, WD repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1409. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00670000098022.
HOGENOMiHOG000007835.
HOVERGENiHBG054128.
InParanoidiQ8BUB4.
OMAiFVLEMEW.
OrthoDBiEOG091G0ANN.
TreeFamiTF314470.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
3.30.40.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00064. FYVE. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BUB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEIQPQPL TRKPILLQRV EGSQEVVNMA VIVPKEEGVI SVSEDRTVRV
60 70 80 90 100
WLKRDSGQYW PSIYHAMPSP CSCMSFNPET RRLSIGLDNG TISEFILSED
110 120 130 140 150
YNKMTPVKNY QAHQSRVTMV LFVLELEWVL STGQDKQFAW HCSESGQRLG
160 170 180 190 200
GYRTSAVASG LQFDVETRHV FIGDHSGQVT ILKLEQENCT LLTSFRGHTG
210 220 230 240 250
GVTALCWDPV QRVLFSGSSD HSVIMWDIGG RKGTAIELQG HNDKVQALSY
260 270 280 290 300
AQHTRQLISC GGDGGIVVWN MDVERQETPE WLDSDSCQKC DQPFFWNFKQ
310 320 330 340 350
MWDSKKIGLR QHHCRKCGKA VCGKCSSKRS SIPLMGFEFE VRVCDSCHEA
360 370 380 390 400
ITDEERAPTA TFHDSKHNIV HVHFDATRGW LLTSGTDKVI KLWDMTPVVS
Length:400
Mass (Da):45,094
Last modified:July 27, 2011 - v2
Checksum:iB6CF4F4F0C11E1F9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti298F → V in BAC39640 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK086275 mRNA. Translation: BAC39640.1.
AK131958 mRNA. Translation: BAE20902.1.
AK151817 mRNA. Translation: BAE30714.1.
CH466535 Genomic DNA. Translation: EDL36078.1.
BC116632 mRNA. Translation: AAI16633.1.
CCDSiCCDS27192.1.
RefSeqiNP_780755.2. NM_175546.4.
UniGeneiMm.1960.
Mm.416426.
Mm.482243.

Genome annotation databases

EnsembliENSMUST00000014691; ENSMUSP00000014691; ENSMUSG00000014547.
GeneIDi268752.
KEGGimmu:268752.
UCSCiuc007ugy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK086275 mRNA. Translation: BAC39640.1.
AK131958 mRNA. Translation: BAE20902.1.
AK151817 mRNA. Translation: BAE30714.1.
CH466535 Genomic DNA. Translation: EDL36078.1.
BC116632 mRNA. Translation: AAI16633.1.
CCDSiCCDS27192.1.
RefSeqiNP_780755.2. NM_175546.4.
UniGeneiMm.1960.
Mm.416426.
Mm.482243.

3D structure databases

ProteinModelPortaliQ8BUB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234549. 1 interactor.
IntActiQ8BUB4. 2 interactors.
STRINGi10090.ENSMUSP00000014691.

PTM databases

PhosphoSitePlusiQ8BUB4.

Proteomic databases

MaxQBiQ8BUB4.
PaxDbiQ8BUB4.
PeptideAtlasiQ8BUB4.
PRIDEiQ8BUB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014691; ENSMUSP00000014691; ENSMUSG00000014547.
GeneIDi268752.
KEGGimmu:268752.
UCSCiuc007ugy.2. mouse.

Organism-specific databases

CTDi115825.
MGIiMGI:2442811. Wdfy2.

Phylogenomic databases

eggNOGiKOG1409. Eukaryota.
COG2319. LUCA.
GeneTreeiENSGT00670000098022.
HOGENOMiHOG000007835.
HOVERGENiHBG054128.
InParanoidiQ8BUB4.
OMAiFVLEMEW.
OrthoDBiEOG091G0ANN.
TreeFamiTF314470.

Miscellaneous databases

ChiTaRSiWdfy2. mouse.
PROiQ8BUB4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000014547.
CleanExiMM_WDFY2.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
3.30.40.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00400. WD40. 3 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00064. FYVE. 1 hit.
SM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWDFY2_MOUSE
AccessioniPrimary (citable) accession number: Q8BUB4
Secondary accession number(s): Q3U9F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.