Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acetolactate synthase-like protein

Gene

Ilvbl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
  • thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981Thiamine pyrophosphateBy similarity
Metal bindingi521 – 5211MagnesiumBy similarity
Metal bindingi547 – 5471MagnesiumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Magnesium, Metal-binding, Thiamine pyrophosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Acetolactate synthase-like protein (EC:2.2.1.-)
Alternative name(s):
IlvB-like protein
Gene namesi
Name:Ilvbl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1351911. Ilvbl.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei13 – 3321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 632632Acetolactate synthase-like proteinPRO_0000314826Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei369 – 3691PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BU33.
MaxQBiQ8BU33.
PaxDbiQ8BU33.
PRIDEiQ8BU33.

PTM databases

iPTMnetiQ8BU33.
PhosphoSiteiQ8BU33.

Expressioni

Gene expression databases

BgeeiQ8BU33.
CleanExiMM_ILVBL.
GenevisibleiQ8BU33. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8BU33. 3 interactions.
MINTiMINT-4112517.
STRINGi10090.ENSMUSP00000101023.

Structurei

3D structure databases

ProteinModelPortaliQ8BU33.
SMRiQ8BU33. Positions 54-586.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni470 – 55081Thiamine pyrophosphate bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the TPP enzyme family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1185. Eukaryota.
COG0028. LUCA.
GeneTreeiENSGT00530000063412.
HOGENOMiHOG000010642.
HOVERGENiHBG108004.
InParanoidiQ8BU33.
KOiK11259.
OMAiKGGFEIP.
OrthoDBiEOG75B84X.
PhylomeDBiQ8BU33.
TreeFamiTF354221.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 2 hits.
SSF52518. SSF52518. 2 hits.
PROSITEiPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BU33-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METSAAAASA GGFFPSFLLL AFGTLVAAVL GVAHRLGLFY QLMHKVDKTS
60 70 80 90 100
IRHGGESVAA VLRAHGVRFV FTLVGGHISP LLVACEKLGI RVVDTRHEVT
110 120 130 140 150
AVFAADAVAR LTGTVGVAAV TAGPGLTNTV TAVKNAQVAQ SPVLLLGGAA
160 170 180 190 200
STLLQKRGAL QAIDQMSLFR PLCKFCASVR RVRDIVPTLR TAIAAAQSGT
210 220 230 240 250
PGPVFVELPL DVLYPYFMVE KEMIPTKLPN SLMGRVVVWY LQNCLANLFV
260 270 280 290 300
GAWEPRPEGP LPLDIPQASP QQVQRCVEIL SRAKRPLLVL GSQALLPPTP
310 320 330 340 350
ANKLRAAVET LGVPCFLGGM SRGLLGRNHP LHIRQNRSAA LKKADVVVLA
360 370 380 390 400
GAVCDFRLSY GRVLNRKSSI IIVNRNRDDL LLNSDIFWKP QEAVQGDVGS
410 420 430 440 450
FMIKLVEGLQ GQMWSSDWAE ELRKADQQKE QTYRDKALMP VLQHLNPVWV
460 470 480 490 500
LQQVEETLPD NALLVVDGGD FVATAAYLVQ PRGPLRWLDP GAFGTLGVGA
510 520 530 540 550
GFALGAKLCQ PEAEVWCLFG DGAFGYSLIE FDTFVRHKVP VIALVGNDAG
560 570 580 590 600
WTQISREQVP RLGSDVACSL AYTDYHKAAM GLGAQGLILS RDNKDQVVKV
610 620 630
LREGQQLCQD GHAVVVNILI GRTDFRDGSI SV
Length:632
Mass (Da):68,156
Last modified:March 1, 2003 - v1
Checksum:i02B5C5ED36ECDE89
GO
Isoform 2 (identifier: Q8BU33-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-416: DVGSFMIKLVEGLQGQMWSS → ECTPRDPTMCMVSSSCLCCI
     417-632: Missing.

Note: No experimental confirmation available.
Show »
Length:416
Mass (Da):44,550
Checksum:i2F6AF90E9154B5DC
GO
Isoform 3 (identifier: Q8BU33-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     396-401: GDVGSF → AGPASP
     402-632: Missing.

Note: No experimental confirmation available.
Show »
Length:401
Mass (Da):42,812
Checksum:i66B7E30E903AE2B0
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei396 – 4016GDVGSF → AGPASP in isoform 3. 1 PublicationVSP_030392
Alternative sequencei397 – 41620DVGSF…QMWSS → ECTPRDPTMCMVSSSCLCCI in isoform 2. 1 PublicationVSP_030393Add
BLAST
Alternative sequencei402 – 632231Missing in isoform 3. 1 PublicationVSP_030394Add
BLAST
Alternative sequencei417 – 632216Missing in isoform 2. 1 PublicationVSP_030395Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087956 mRNA. Translation: BAC40057.1.
AK133553 mRNA. Translation: BAE21720.1.
AK143918 mRNA. Translation: BAE25603.1.
AK146075 mRNA. Translation: BAE26879.1.
BC056459 mRNA. Translation: AAH56459.1.
CCDSiCCDS23966.1. [Q8BU33-1]
RefSeqiNP_776112.1. NM_173751.4. [Q8BU33-1]
XP_011241702.1. XM_011243400.1. [Q8BU33-1]
XP_011241703.1. XM_011243401.1. [Q8BU33-3]
UniGeneiMm.2644.

Genome annotation databases

EnsembliENSMUST00000105384; ENSMUSP00000101023; ENSMUSG00000032763. [Q8BU33-1]
GeneIDi216136.
KEGGimmu:216136.
UCSCiuc007fxv.2. mouse. [Q8BU33-1]
uc007fxw.1. mouse. [Q8BU33-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087956 mRNA. Translation: BAC40057.1.
AK133553 mRNA. Translation: BAE21720.1.
AK143918 mRNA. Translation: BAE25603.1.
AK146075 mRNA. Translation: BAE26879.1.
BC056459 mRNA. Translation: AAH56459.1.
CCDSiCCDS23966.1. [Q8BU33-1]
RefSeqiNP_776112.1. NM_173751.4. [Q8BU33-1]
XP_011241702.1. XM_011243400.1. [Q8BU33-1]
XP_011241703.1. XM_011243401.1. [Q8BU33-3]
UniGeneiMm.2644.

3D structure databases

ProteinModelPortaliQ8BU33.
SMRiQ8BU33. Positions 54-586.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BU33. 3 interactions.
MINTiMINT-4112517.
STRINGi10090.ENSMUSP00000101023.

PTM databases

iPTMnetiQ8BU33.
PhosphoSiteiQ8BU33.

Proteomic databases

EPDiQ8BU33.
MaxQBiQ8BU33.
PaxDbiQ8BU33.
PRIDEiQ8BU33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000105384; ENSMUSP00000101023; ENSMUSG00000032763. [Q8BU33-1]
GeneIDi216136.
KEGGimmu:216136.
UCSCiuc007fxv.2. mouse. [Q8BU33-1]
uc007fxw.1. mouse. [Q8BU33-2]

Organism-specific databases

CTDi10994.
MGIiMGI:1351911. Ilvbl.

Phylogenomic databases

eggNOGiKOG1185. Eukaryota.
COG0028. LUCA.
GeneTreeiENSGT00530000063412.
HOGENOMiHOG000010642.
HOVERGENiHBG108004.
InParanoidiQ8BU33.
KOiK11259.
OMAiKGGFEIP.
OrthoDBiEOG75B84X.
PhylomeDBiQ8BU33.
TreeFamiTF354221.

Miscellaneous databases

PROiQ8BU33.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BU33.
CleanExiMM_ILVBL.
GenevisibleiQ8BU33. MM.

Family and domain databases

Gene3Di3.40.50.1220. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR029035. DHS-like_NAD/FAD-binding_dom.
IPR029061. THDP-binding.
IPR012000. Thiamin_PyroP_enz_cen_dom.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
IPR000399. TPP-bd_CS.
IPR011766. TPP_enzyme-bd_C.
[Graphical view]
PfamiPF02775. TPP_enzyme_C. 1 hit.
PF00205. TPP_enzyme_M. 1 hit.
PF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
SUPFAMiSSF52467. SSF52467. 2 hits.
SSF52518. SSF52518. 2 hits.
PROSITEiPS00187. TPP_ENZYMES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Kidney, Liver, Pituitary and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-369, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiILVBL_MOUSE
AccessioniPrimary (citable) accession number: Q8BU33
Secondary accession number(s): Q3UNZ4, Q3UZY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.