Q8BU33 (ILVBL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetolactate synthase-like protein
EC=2.2.1.-

Alternative name(s):
IlvB-like protein

Gene names

Name:

Ilvbl

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	632 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Subcellular location	Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: InterPro thiamine pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BU33-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BU33-2)

The sequence of this isoform differs from the canonical sequence as follows:
     397-416: DVGSFMIKLVEGLQGQMWSS → ECTPRDPTMCMVSSSCLCCI
     417-632: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8BU33-3)

The sequence of this isoform differs from the canonical sequence as follows:
     396-401: GDVGSF → AGPASP
     402-632: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 632	632	Acetolactate synthase-like protein		PRO_0000314826
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Region	470 – 550	81	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	521	1	Magnesium By similarity
	Metal binding	547	1	Magnesium By similarity
	Binding site	98	1	Thiamine pyrophosphate By similarity
Natural variations
	Alternative sequence	396 – 401	6	GDVGSF → AGPASP in isoform 3.		VSP_030392
	Alternative sequence	397 – 416	20	DVGSF…QMWSS → ECTPRDPTMCMVSSSCLCCI in isoform 2.		VSP_030393
	Alternative sequence	402 – 632	231	Missing in isoform 3.		VSP_030394
	Alternative sequence	417 – 632	216	Missing in isoform 2.		VSP_030395

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 02B5C5ED36ECDE89	FASTA	632	68,156
	10 20 30 40 50 60 METSAAAASA GGFFPSFLLL AFGTLVAAVL GVAHRLGLFY QLMHKVDKTS IRHGGESVAA 70 80 90 100 110 120 VLRAHGVRFV FTLVGGHISP LLVACEKLGI RVVDTRHEVT AVFAADAVAR LTGTVGVAAV 130 140 150 160 170 180 TAGPGLTNTV TAVKNAQVAQ SPVLLLGGAA STLLQKRGAL QAIDQMSLFR PLCKFCASVR 190 200 210 220 230 240 RVRDIVPTLR TAIAAAQSGT PGPVFVELPL DVLYPYFMVE KEMIPTKLPN SLMGRVVVWY 250 260 270 280 290 300 LQNCLANLFV GAWEPRPEGP LPLDIPQASP QQVQRCVEIL SRAKRPLLVL GSQALLPPTP 310 320 330 340 350 360 ANKLRAAVET LGVPCFLGGM SRGLLGRNHP LHIRQNRSAA LKKADVVVLA GAVCDFRLSY 370 380 390 400 410 420 GRVLNRKSSI IIVNRNRDDL LLNSDIFWKP QEAVQGDVGS FMIKLVEGLQ GQMWSSDWAE 430 440 450 460 470 480 ELRKADQQKE QTYRDKALMP VLQHLNPVWV LQQVEETLPD NALLVVDGGD FVATAAYLVQ 490 500 510 520 530 540 PRGPLRWLDP GAFGTLGVGA GFALGAKLCQ PEAEVWCLFG DGAFGYSLIE FDTFVRHKVP 550 560 570 580 590 600 VIALVGNDAG WTQISREQVP RLGSDVACSL AYTDYHKAAM GLGAQGLILS RDNKDQVVKV 610 620 630 LREGQQLCQD GHAVVVNILI GRTDFRDGSI SV
	Isoform 2 [UniParc]. Checksum: 2F6AF90E9154B5DC	FASTA	416	44,550
	10 20 30 40 50 60 METSAAAASA GGFFPSFLLL AFGTLVAAVL GVAHRLGLFY QLMHKVDKTS IRHGGESVAA 70 80 90 100 110 120 VLRAHGVRFV FTLVGGHISP LLVACEKLGI RVVDTRHEVT AVFAADAVAR LTGTVGVAAV 130 140 150 160 170 180 TAGPGLTNTV TAVKNAQVAQ SPVLLLGGAA STLLQKRGAL QAIDQMSLFR PLCKFCASVR 190 200 210 220 230 240 RVRDIVPTLR TAIAAAQSGT PGPVFVELPL DVLYPYFMVE KEMIPTKLPN SLMGRVVVWY 250 260 270 280 290 300 LQNCLANLFV GAWEPRPEGP LPLDIPQASP QQVQRCVEIL SRAKRPLLVL GSQALLPPTP 310 320 330 340 350 360 ANKLRAAVET LGVPCFLGGM SRGLLGRNHP LHIRQNRSAA LKKADVVVLA GAVCDFRLSY 370 380 390 400 410 GRVLNRKSSI IIVNRNRDDL LLNSDIFWKP QEAVQGECTP RDPTMCMVSS SCLCCI
	Isoform 3 [UniParc]. Checksum: 66B7E30E903AE2B0	FASTA	401	42,812
	10 20 30 40 50 60 METSAAAASA GGFFPSFLLL AFGTLVAAVL GVAHRLGLFY QLMHKVDKTS IRHGGESVAA 70 80 90 100 110 120 VLRAHGVRFV FTLVGGHISP LLVACEKLGI RVVDTRHEVT AVFAADAVAR LTGTVGVAAV 130 140 150 160 170 180 TAGPGLTNTV TAVKNAQVAQ SPVLLLGGAA STLLQKRGAL QAIDQMSLFR PLCKFCASVR 190 200 210 220 230 240 RVRDIVPTLR TAIAAAQSGT PGPVFVELPL DVLYPYFMVE KEMIPTKLPN SLMGRVVVWY 250 260 270 280 290 300 LQNCLANLFV GAWEPRPEGP LPLDIPQASP QQVQRCVEIL SRAKRPLLVL GSQALLPPTP 310 320 330 340 350 360 ANKLRAAVET LGVPCFLGGM SRGLLGRNHP LHIRQNRSAA LKKADVVVLA GAVCDFRLSY 370 380 390 400 GRVLNRKSSI IIVNRNRDDL LLNSDIFWKP QEAVQAGPAS P

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6J and NOD. Tissue: Kidney, Liver, Pituitary and Thymus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK087956 mRNA. Translation: BAC40057.1. AK133553 mRNA. Translation: BAE21720.1. AK143918 mRNA. Translation: BAE25603.1. AK146075 mRNA. Translation: BAE26879.1. BC056459 mRNA. Translation: AAH56459.1.
IPI	IPI00225209. IPI00881209. IPI00881346.
RefSeq	NP_776112.1. NM_173751.4.
UniGene	Mm.2644.
3D structure databases
HSSP	HSSP built from PDB template 1OZH based on UniProtKB P27696.
ProteinModelPortal	Q8BU33.
SMR	Q8BU33. Positions 50-628.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BU33.
PTM databases
PhosphoSite	Q8BU33.
Proteomic databases
PRIDE	Q8BU33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000105384; ENSMUSP00000101023; ENSMUSG00000032763. ENSMUST00000172436; ENSMUSP00000125793; ENSMUSG00000032763.
GeneID	216136.
KEGG	mmu:216136.
UCSC	uc007fxv.2. mouse. uc007fxw.1. mouse.
Organism-specific databases
CTD	10994.
MGI	MGI:1351911. Ilvbl.
Phylogenomic databases
eggNOG	roNOG06097.
GeneTree	ENSGT00530000063412.
HOGENOM	HBG323037.
HOVERGEN	HBG108004.
InParanoid	Q8BU33.
OMA	FLGGMSR.
OrthoDB	EOG48D0V2.
PhylomeDB	Q8BU33.
Gene expression databases
ArrayExpress	Q8BU33.
Bgee	Q8BU33.
CleanEx	MM_ILVBL.
Genevestigator	Q8BU33.
Family and domain databases
InterPro	IPR012000. Thiamin_PyroP_enz_cen_dom. IPR012001. Thiamin_PyroP_enz_TPP-bd_dom. IPR000399. TPP-bd_CS. IPR011766. TPP_enzyme-bd_C. [Graphical view]
KO	K11259.
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	374994.
SOURCE	Search...

Entry information

Entry name

ILVBL_MOUSE

Accession

Primary (citable) accession number: Q8BU33
Secondary accession number(s): Q3UNZ4, Q3UZY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2003
Last modified:	November 16, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program