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Protein

Ras-related protein Rap-2c

Gene

Rap2c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. May play a role in cytoskeletal rearrangements and regulate cell spreading through activation of the effector TNIK. May play a role in SRE-mediated gene transcription.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 178GTPBy similarity
Nucleotide bindingi57 – 615GTPBy similarity
Nucleotide bindingi116 – 1194GTPBy similarity

GO - Molecular functioni

GO - Biological processi

  • establishment of endothelial intestinal barrier Source: MGI
  • negative regulation of cell migration Source: MGI
  • positive regulation of protein autophosphorylation Source: MGI
  • protein transport Source: InterPro
  • Rap protein signal transduction Source: UniProtKB
  • regulation of nucleic acid-templated transcription Source: MGI
  • regulation of protein tyrosine kinase activity Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rap-2c
Gene namesi
Name:Rap2c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1919315. Rap2c.

Subcellular locationi

GO - Cellular componenti

  • bicellular tight junction Source: MGI
  • cell-cell contact zone Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: MGI
  • recycling endosome Source: MGI
  • recycling endosome membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi176 – 1761C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-177. 1 Publication
Mutagenesisi177 – 1771C → G: Loss of association with the recycling endosome membranes and loss of TNIK activation; when associated with C-176. 1 Publication
Mutagenesisi180 – 1801C → A: Loss of association with membranes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 180180Ras-related protein Rap-2cPRO_0000030223Add
BLAST
Propeptidei181 – 1833Removed in mature form1 PublicationPRO_0000030224

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi176 – 1761S-palmitoyl cysteine1 Publication
Lipidationi177 – 1771S-palmitoyl cysteine1 Publication
Modified residuei180 – 1801Cysteine methyl ester1 Publication
Lipidationi180 – 1801S-geranylgeranyl cysteine1 Publication

Post-translational modificationi

Palmitoylated. Palmitoylation is required for association with recycling endosome membranes and activation of TNIK.1 Publication

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

EPDiQ8BU31.
MaxQBiQ8BU31.
PaxDbiQ8BU31.
PRIDEiQ8BU31.

PTM databases

iPTMnetiQ8BU31.
PhosphoSiteiQ8BU31.
SwissPalmiQ8BU31.

Expressioni

Gene expression databases

BgeeiQ8BU31.
CleanExiMM_RAP2C.
GenevisibleiQ8BU31. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058391.

Structurei

3D structure databases

ProteinModelPortaliQ8BU31.
SMRiQ8BU31. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 409Effector regionCurated

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ras family.Curated

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ8BU31.
KOiK07839.
OMAiKEKKSYC.
OrthoDBiEOG7QVM41.
PhylomeDBiQ8BU31.
TreeFamiTF313014.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BU31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREYKVVVLG SGGVGKSALT VQFVTGTFIE KYDPTIEDFY RKEIEVDSSP
60 70 80 90 100
SVLEILDTAG TEQFASMRDL YIKNGQGFIL VYSLVNQQSF QDIKPMRDQI
110 120 130 140 150
VRVKRYEKVP LILVGNKVDL EPEREVMSSE GRALAQEWGC PFMETSAKSK
160 170 180
SMVDELFAEI VRQMNYSSLP EKQDQCCTTC VVQ
Length:183
Mass (Da):20,745
Last modified:March 1, 2003 - v1
Checksum:i6763385F76638324
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti83 – 831S → N in AAH27363 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087971 mRNA. Translation: BAC40066.1.
AK146057 mRNA. Translation: BAE26866.1.
AK146956 mRNA. Translation: BAE27564.1.
AK147015 mRNA. Translation: BAE27609.1.
BC027363 mRNA. Translation: AAH27363.3.
BC050056 mRNA. Translation: AAH50056.2.
BC064814 mRNA. Translation: AAH64814.2.
BC094890 mRNA. Translation: AAH94890.1.
CCDSiCCDS30121.1.
RefSeqiNP_766001.1. NM_172413.2.
XP_006541608.1. XM_006541545.2.
XP_006541609.1. XM_006541546.2.
UniGeneiMm.43152.

Genome annotation databases

EnsembliENSMUST00000053593; ENSMUSP00000058391; ENSMUSG00000050029.
GeneIDi72065.
KEGGimmu:72065.
UCSCiuc009tds.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087971 mRNA. Translation: BAC40066.1.
AK146057 mRNA. Translation: BAE26866.1.
AK146956 mRNA. Translation: BAE27564.1.
AK147015 mRNA. Translation: BAE27609.1.
BC027363 mRNA. Translation: AAH27363.3.
BC050056 mRNA. Translation: AAH50056.2.
BC064814 mRNA. Translation: AAH64814.2.
BC094890 mRNA. Translation: AAH94890.1.
CCDSiCCDS30121.1.
RefSeqiNP_766001.1. NM_172413.2.
XP_006541608.1. XM_006541545.2.
XP_006541609.1. XM_006541546.2.
UniGeneiMm.43152.

3D structure databases

ProteinModelPortaliQ8BU31.
SMRiQ8BU31. Positions 1-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000058391.

PTM databases

iPTMnetiQ8BU31.
PhosphoSiteiQ8BU31.
SwissPalmiQ8BU31.

Proteomic databases

EPDiQ8BU31.
MaxQBiQ8BU31.
PaxDbiQ8BU31.
PRIDEiQ8BU31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000053593; ENSMUSP00000058391; ENSMUSG00000050029.
GeneIDi72065.
KEGGimmu:72065.
UCSCiuc009tds.1. mouse.

Organism-specific databases

CTDi57826.
MGIiMGI:1919315. Rap2c.

Phylogenomic databases

eggNOGiKOG0395. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00780000121857.
HOGENOMiHOG000233973.
HOVERGENiHBG009351.
InParanoidiQ8BU31.
KOiK07839.
OMAiKEKKSYC.
OrthoDBiEOG7QVM41.
PhylomeDBiQ8BU31.
TreeFamiTF313014.

Miscellaneous databases

PROiQ8BU31.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BU31.
CleanExiMM_RAP2C.
GenevisibleiQ8BU31. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryonic heart, Embryonic liver and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and FVB/N.
    Tissue: Embryo, Eye, Limb and Mammary gland.
  3. Cited for: FUNCTION, SUBCELLULAR LOCATION, ISOPRENYLATION AT CYS-180, PALMITOYLATION AT CYS-176 AND CYS-177, MUTAGENESIS OF CYS-176; CYS-177 AND CYS-180.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiRAP2C_MOUSE
AccessioniPrimary (citable) accession number: Q8BU31
Secondary accession number(s): Q504Q0
, Q6P1Y7, Q810J4, Q8R2P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2003
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.