Reviewed, UniProtKB/Swiss-Prot Q8BU30 (SYIC_MOUSE)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS
      Short name=IRS

Gene names

Name:

Iars

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1262 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subunit structure	Part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1262	1262	Isoleucyl-tRNA synthetase, cytoplasmic		PRO_0000098598
Regions
	Motif	48 – 58	11	"HIGH" region
	Motif	600 – 604	5	"KMSKS" region
Sites
	Binding site	603	1	ATP By similarity
Amino acid modifications
	Modified residue	844	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8BU30-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D4B004D90C1F32AA	FASTA	1,262	144,257
	10 20 30 40 50 60 MVQQVPENIS FPAEEEKILE FWSKHNCFQE CLKQSKLRPK FTFYDGPPFA TGLPHYGHIL 70 80 90 100 110 120 AGTIKDIVTR YAHQSGFHVD RRFGWDCHGL PVEYEIDKTL GIKGPEDVAK MGIAEYNKQC 130 140 150 160 170 180 RAIVMRYSAE WKSTVTRLGR WIDFDNDYKT LYPQFMESVW WVFKQLYDKG LVYRGVKVMP 190 200 210 220 230 240 FSTACGTPLS NFESNQNYKD VQDPSVFVTF PLEEDENTSL VAWTTTPWTL PSNLALCVNP 250 260 270 280 290 300 EIQYVKIKDV ARGKLFILTE ARLSALYKQE SDYEILERFP GASLKGKKYK PLFDYFIKCK 310 320 330 340 350 360 ENGAFTVLVD HYVKDEEGTG VVHQAPYFGA DDHRVCMDFN IIQKDSVPVC PVDASGCFTE 370 380 390 400 410 420 EVTHFVGQYV KDADKNIIRM LKEQGRLLAA GTFTHSYPFC WRSDTPLIYK SVPSWFVRVE 430 440 450 460 470 480 PMVDQLLKNN DLCYWVPEFV REKRFGNWLK EARDWAISRN RYWGTPIPLW VSEDLEEVVC 490 500 510 520 530 540 IGSVAELEEL SGTKISDLHR ESIDHLTIPS RCGKAPLRRV SEVFDCWFES GSMPYAQVHY 550 560 570 580 590 600 PFESKREFED AFPADFIAEG IDQTRGWFYT LLVLATALFG QPPFKNVIVN GLILASDGQK 610 620 630 640 650 660 MSKRKKNYPD PVSIIDKYGA DALRLYLINS PVVRAENLRF KEEGVRDVLK DVLLPWYNAY 670 680 690 700 710 720 RFFIQNVFRL HKEEEVKFLY NEHTVRESPN ITDRWVLSFM QSLLGFFETE MAAYRLYTVV 730 740 750 760 770 780 PRLVKFVDIL TNWYVRMNRR RLKGESGVED CVMALETLFS VLLSLCRLMA PYTPFLTELM 790 800 810 820 830 840 YQNLKLLIDP ASLRDKDTLS IHYLMLPRVR EELIDKKTEN AVSRMQSVIE LGRVIRDRKT 850 860 870 880 890 900 IPIKYPLKEI VVIHQDPEAL EDIRSLEKYI IEELNVRKVT LSTDKNKYGI RLRAEPDHMV 910 920 930 940 950 960 LGKRLKGAFK AVMMAIKRLS NEELERFQKS GSIVVEGHEL HEEDIRLMYT FDQATGGTAQ 970 980 990 1000 1010 1020 FEAHSDAQAL VLLDVTPDQS MVDEGMAREV INRIQKLRKK CNLVPTDEIT VYYNAKSEGR 1030 1040 1050 1060 1070 1080 YLNNVIESHT DFVFATIKAP LKPYPVPTSD NILIQEQTQL KGSELEITLT KGSCVPGPAC 1090 1100 1110 1120 1130 1140 AYVNLNICAN GTEQGGVLLL ENPKGDNQLN LVKLKTVVTS VFGVKNAKLS VFHGETEIQN 1150 1160 1170 1180 1190 1200 QTDLLSLSGR TLCVTAGASP SPISSPSTLL CQYLNLQLLN AEPQECLTGT VGTLLLENPL 1210 1220 1230 1240 1250 1260 GQNGLTHQGL VHEAAKVFGL RSRRLRLFLN ETQTQEITED IPMKTLNMKT VYVSVLPTTA DG

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Thymus.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK087992 mRNA. Translation: BAC40081.1.
IPI	IPI00225201.
UniGene	Mm.21118
3D structure databases
HSSP	HSSP built from PDB template 1ILE based on UniProtKB P56690.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8BU30.
PTM databases
PhosphoSite	Q8BU30.
Proteomic databases
PRIDE	Q8BU30.
Genome annotation databases
Ensembl	ENSMUST00000047363; ENSMUSP00000048096; ENSMUSG00000037851; Mus musculus. [Genome view]
KEGG	mmu:105148.
UCSC	uc007qjv.1. mouse.
Organism-specific databases
CTD	105148.
MGI	MGI:2145219. Iars.
Phylogenomic databases
HOGENOM	Q8BU30.
HOVERGEN	Q8BU30.
Enzyme and pathway databases
BRENDA	6.1.1.5. 244.
Gene expression databases
ArrayExpress	Q8BU30.
Bgee	Q8BU30.
CleanEx	MM_IARS.
Genevestigator	Q8BU30.
GermOnline	ENSMUSG00000037851. Mus musculus.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	357460.
SOURCE	Search...

Entry information

Entry name

SYIC_MOUSE

Accession

Primary (citable) accession number: Q8BU30

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	March 1, 2003
Last modified:	October 13, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)