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Q8BU30

- SYIC_MOUSE

UniProt

Q8BU30 - SYIC_MOUSE

Protein

Isoleucine--tRNA ligase, cytoplasmic

Gene

Iars

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei603 – 6031ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IRS
    Short name:
    IleRS
    Gene namesi
    Name:Iars
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:2145219. Iars.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12621262Isoleucine--tRNA ligase, cytoplasmicPRO_0000098598Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei1049 – 10491PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8BU30.
    PaxDbiQ8BU30.
    PRIDEiQ8BU30.

    PTM databases

    PhosphoSiteiQ8BU30.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BU30.
    BgeeiQ8BU30.
    CleanExiMM_IARS.
    GenevestigatoriQ8BU30.

    Interactioni

    Subunit structurei

    Part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.

    Protein-protein interaction databases

    BioGridi222774. 5 interactions.
    IntActiQ8BU30. 1 interaction.
    MINTiMINT-1869822.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BU30.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.
    GeneTreeiENSGT00550000074921.
    HOGENOMiHOG000246403.
    HOVERGENiHBG013511.
    InParanoidiQ6NXK4.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG7M98FD.
    PhylomeDBiQ8BU30.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BU30-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVQQVPENIS FPAEEEKILE FWSKHNCFQE CLKQSKLRPK FTFYDGPPFA     50
    TGLPHYGHIL AGTIKDIVTR YAHQSGFHVD RRFGWDCHGL PVEYEIDKTL 100
    GIKGPEDVAK MGIAEYNKQC RAIVMRYSAE WKSTVTRLGR WIDFDNDYKT 150
    LYPQFMESVW WVFKQLYDKG LVYRGVKVMP FSTACGTPLS NFESNQNYKD 200
    VQDPSVFVTF PLEEDENTSL VAWTTTPWTL PSNLALCVNP EIQYVKIKDV 250
    ARGKLFILTE ARLSALYKQE SDYEILERFP GASLKGKKYK PLFDYFIKCK 300
    ENGAFTVLVD HYVKDEEGTG VVHQAPYFGA DDHRVCMDFN IIQKDSVPVC 350
    PVDASGCFTE EVTHFVGQYV KDADKNIIRM LKEQGRLLAA GTFTHSYPFC 400
    WRSDTPLIYK SVPSWFVRVE PMVDQLLKNN DLCYWVPEFV REKRFGNWLK 450
    EARDWAISRN RYWGTPIPLW VSEDLEEVVC IGSVAELEEL SGTKISDLHR 500
    ESIDHLTIPS RCGKAPLRRV SEVFDCWFES GSMPYAQVHY PFESKREFED 550
    AFPADFIAEG IDQTRGWFYT LLVLATALFG QPPFKNVIVN GLILASDGQK 600
    MSKRKKNYPD PVSIIDKYGA DALRLYLINS PVVRAENLRF KEEGVRDVLK 650
    DVLLPWYNAY RFFIQNVFRL HKEEEVKFLY NEHTVRESPN ITDRWVLSFM 700
    QSLLGFFETE MAAYRLYTVV PRLVKFVDIL TNWYVRMNRR RLKGESGVED 750
    CVMALETLFS VLLSLCRLMA PYTPFLTELM YQNLKLLIDP ASLRDKDTLS 800
    IHYLMLPRVR EELIDKKTEN AVSRMQSVIE LGRVIRDRKT IPIKYPLKEI 850
    VVIHQDPEAL EDIRSLEKYI IEELNVRKVT LSTDKNKYGI RLRAEPDHMV 900
    LGKRLKGAFK AVMMAIKRLS NEELERFQKS GSIVVEGHEL HEEDIRLMYT 950
    FDQATGGTAQ FEAHSDAQAL VLLDVTPDQS MVDEGMAREV INRIQKLRKK 1000
    CNLVPTDEIT VYYNAKSEGR YLNNVIESHT DFIFATIKAP LKPYPVPTSD 1050
    NILIQEQTQL KGSELEITLT KGSCVPGPAC AYVNLNICAN GTEQGGVLLL 1100
    ENPKGDNQLN LVKLKTVVTS VFGVKNAKLS VFHGETEIQN QTDLLSLSGR 1150
    TLCVTAGASP SPISSPSTLL CQYLNLQLLN AEPQECLTGT VGTLLLENPL 1200
    GQNGLTHQGL VHEAAKVFGL RSRRLRLFLN ETQTQEITED IPMKTLNMKT 1250
    VYVSVLPTTA DG 1262
    Length:1,262
    Mass (Da):144,271
    Last modified:July 27, 2011 - v2
    Checksum:i2039614F557E1312
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1033 – 10331I → V in BAC40081. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK087992 mRNA. Translation: BAC40081.1.
    AK133856 mRNA. Translation: BAE21890.1.
    CH466546 Genomic DNA. Translation: EDL41088.1.
    BC067029 mRNA. Translation: AAH67029.1.
    CCDSiCCDS36659.1.
    RefSeqiNP_742012.2. NM_172015.3.
    XP_006516889.1. XM_006516826.1.
    UniGeneiMm.21118.

    Genome annotation databases

    EnsembliENSMUST00000047363; ENSMUSP00000048096; ENSMUSG00000037851.
    ENSMUST00000164260; ENSMUSP00000126806; ENSMUSG00000037851.
    ENSMUST00000165316; ENSMUSP00000132082; ENSMUSG00000037851.
    GeneIDi105148.
    KEGGimmu:105148.
    UCSCiuc007qjv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK087992 mRNA. Translation: BAC40081.1 .
    AK133856 mRNA. Translation: BAE21890.1 .
    CH466546 Genomic DNA. Translation: EDL41088.1 .
    BC067029 mRNA. Translation: AAH67029.1 .
    CCDSi CCDS36659.1.
    RefSeqi NP_742012.2. NM_172015.3.
    XP_006516889.1. XM_006516826.1.
    UniGenei Mm.21118.

    3D structure databases

    ProteinModelPortali Q8BU30.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 222774. 5 interactions.
    IntActi Q8BU30. 1 interaction.
    MINTi MINT-1869822.

    PTM databases

    PhosphoSitei Q8BU30.

    Proteomic databases

    MaxQBi Q8BU30.
    PaxDbi Q8BU30.
    PRIDEi Q8BU30.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047363 ; ENSMUSP00000048096 ; ENSMUSG00000037851 .
    ENSMUST00000164260 ; ENSMUSP00000126806 ; ENSMUSG00000037851 .
    ENSMUST00000165316 ; ENSMUSP00000132082 ; ENSMUSG00000037851 .
    GeneIDi 105148.
    KEGGi mmu:105148.
    UCSCi uc007qjv.2. mouse.

    Organism-specific databases

    CTDi 3376.
    MGIi MGI:2145219. Iars.

    Phylogenomic databases

    eggNOGi COG0060.
    GeneTreei ENSGT00550000074921.
    HOGENOMi HOG000246403.
    HOVERGENi HBG013511.
    InParanoidi Q6NXK4.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG7M98FD.
    PhylomeDBi Q8BU30.

    Miscellaneous databases

    ChiTaRSi IARS. mouse.
    NextBioi 357460.
    PROi Q8BU30.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BU30.
    Bgeei Q8BU30.
    CleanExi MM_IARS.
    Genevestigatori Q8BU30.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Embryo and Thymus.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiSYIC_MOUSE
    AccessioniPrimary (citable) accession number: Q8BU30
    Secondary accession number(s): Q6NXK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 91 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3