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Q8BU30

- SYIC_MOUSE

UniProt

Q8BU30 - SYIC_MOUSE

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Protein

Isoleucine--tRNA ligase, cytoplasmic

Gene

Iars

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei603 – 6031ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: InterPro
  2. osteoblast differentiation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IRS
Short name:
IleRS
Gene namesi
Name:Iars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:2145219. Iars.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12621262Isoleucine--tRNA ligase, cytoplasmicPRO_0000098598Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei1049 – 10491PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BU30.
PaxDbiQ8BU30.
PRIDEiQ8BU30.

PTM databases

PhosphoSiteiQ8BU30.

Expressioni

Gene expression databases

BgeeiQ8BU30.
CleanExiMM_IARS.
ExpressionAtlasiQ8BU30. baseline and differential.
GenevestigatoriQ8BU30.

Interactioni

Subunit structurei

Part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.

Protein-protein interaction databases

BioGridi222774. 5 interactions.
IntActiQ8BU30. 1 interaction.
MINTiMINT-1869822.

Structurei

3D structure databases

ProteinModelPortaliQ8BU30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074921.
HOGENOMiHOG000246403.
HOVERGENiHBG013511.
InParanoidiQ8BU30.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG7M98FD.
PhylomeDBiQ8BU30.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BU30-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVQQVPENIS FPAEEEKILE FWSKHNCFQE CLKQSKLRPK FTFYDGPPFA
60 70 80 90 100
TGLPHYGHIL AGTIKDIVTR YAHQSGFHVD RRFGWDCHGL PVEYEIDKTL
110 120 130 140 150
GIKGPEDVAK MGIAEYNKQC RAIVMRYSAE WKSTVTRLGR WIDFDNDYKT
160 170 180 190 200
LYPQFMESVW WVFKQLYDKG LVYRGVKVMP FSTACGTPLS NFESNQNYKD
210 220 230 240 250
VQDPSVFVTF PLEEDENTSL VAWTTTPWTL PSNLALCVNP EIQYVKIKDV
260 270 280 290 300
ARGKLFILTE ARLSALYKQE SDYEILERFP GASLKGKKYK PLFDYFIKCK
310 320 330 340 350
ENGAFTVLVD HYVKDEEGTG VVHQAPYFGA DDHRVCMDFN IIQKDSVPVC
360 370 380 390 400
PVDASGCFTE EVTHFVGQYV KDADKNIIRM LKEQGRLLAA GTFTHSYPFC
410 420 430 440 450
WRSDTPLIYK SVPSWFVRVE PMVDQLLKNN DLCYWVPEFV REKRFGNWLK
460 470 480 490 500
EARDWAISRN RYWGTPIPLW VSEDLEEVVC IGSVAELEEL SGTKISDLHR
510 520 530 540 550
ESIDHLTIPS RCGKAPLRRV SEVFDCWFES GSMPYAQVHY PFESKREFED
560 570 580 590 600
AFPADFIAEG IDQTRGWFYT LLVLATALFG QPPFKNVIVN GLILASDGQK
610 620 630 640 650
MSKRKKNYPD PVSIIDKYGA DALRLYLINS PVVRAENLRF KEEGVRDVLK
660 670 680 690 700
DVLLPWYNAY RFFIQNVFRL HKEEEVKFLY NEHTVRESPN ITDRWVLSFM
710 720 730 740 750
QSLLGFFETE MAAYRLYTVV PRLVKFVDIL TNWYVRMNRR RLKGESGVED
760 770 780 790 800
CVMALETLFS VLLSLCRLMA PYTPFLTELM YQNLKLLIDP ASLRDKDTLS
810 820 830 840 850
IHYLMLPRVR EELIDKKTEN AVSRMQSVIE LGRVIRDRKT IPIKYPLKEI
860 870 880 890 900
VVIHQDPEAL EDIRSLEKYI IEELNVRKVT LSTDKNKYGI RLRAEPDHMV
910 920 930 940 950
LGKRLKGAFK AVMMAIKRLS NEELERFQKS GSIVVEGHEL HEEDIRLMYT
960 970 980 990 1000
FDQATGGTAQ FEAHSDAQAL VLLDVTPDQS MVDEGMAREV INRIQKLRKK
1010 1020 1030 1040 1050
CNLVPTDEIT VYYNAKSEGR YLNNVIESHT DFIFATIKAP LKPYPVPTSD
1060 1070 1080 1090 1100
NILIQEQTQL KGSELEITLT KGSCVPGPAC AYVNLNICAN GTEQGGVLLL
1110 1120 1130 1140 1150
ENPKGDNQLN LVKLKTVVTS VFGVKNAKLS VFHGETEIQN QTDLLSLSGR
1160 1170 1180 1190 1200
TLCVTAGASP SPISSPSTLL CQYLNLQLLN AEPQECLTGT VGTLLLENPL
1210 1220 1230 1240 1250
GQNGLTHQGL VHEAAKVFGL RSRRLRLFLN ETQTQEITED IPMKTLNMKT
1260
VYVSVLPTTA DG
Length:1,262
Mass (Da):144,271
Last modified:July 27, 2011 - v2
Checksum:i2039614F557E1312
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1033 – 10331I → V in BAC40081. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087992 mRNA. Translation: BAC40081.1.
AK133856 mRNA. Translation: BAE21890.1.
CH466546 Genomic DNA. Translation: EDL41088.1.
BC067029 mRNA. Translation: AAH67029.1.
CCDSiCCDS36659.1.
RefSeqiNP_742012.2. NM_172015.3.
XP_006516889.1. XM_006516826.1.
UniGeneiMm.21118.

Genome annotation databases

EnsembliENSMUST00000047363; ENSMUSP00000048096; ENSMUSG00000037851.
ENSMUST00000164260; ENSMUSP00000126806; ENSMUSG00000037851.
ENSMUST00000165316; ENSMUSP00000132082; ENSMUSG00000037851.
GeneIDi105148.
KEGGimmu:105148.
UCSCiuc007qjv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087992 mRNA. Translation: BAC40081.1 .
AK133856 mRNA. Translation: BAE21890.1 .
CH466546 Genomic DNA. Translation: EDL41088.1 .
BC067029 mRNA. Translation: AAH67029.1 .
CCDSi CCDS36659.1.
RefSeqi NP_742012.2. NM_172015.3.
XP_006516889.1. XM_006516826.1.
UniGenei Mm.21118.

3D structure databases

ProteinModelPortali Q8BU30.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 222774. 5 interactions.
IntActi Q8BU30. 1 interaction.
MINTi MINT-1869822.

PTM databases

PhosphoSitei Q8BU30.

Proteomic databases

MaxQBi Q8BU30.
PaxDbi Q8BU30.
PRIDEi Q8BU30.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047363 ; ENSMUSP00000048096 ; ENSMUSG00000037851 .
ENSMUST00000164260 ; ENSMUSP00000126806 ; ENSMUSG00000037851 .
ENSMUST00000165316 ; ENSMUSP00000132082 ; ENSMUSG00000037851 .
GeneIDi 105148.
KEGGi mmu:105148.
UCSCi uc007qjv.2. mouse.

Organism-specific databases

CTDi 3376.
MGIi MGI:2145219. Iars.

Phylogenomic databases

eggNOGi COG0060.
GeneTreei ENSGT00550000074921.
HOGENOMi HOG000246403.
HOVERGENi HBG013511.
InParanoidi Q8BU30.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG7M98FD.
PhylomeDBi Q8BU30.

Miscellaneous databases

ChiTaRSi Iars. mouse.
NextBioi 357460.
PROi Q8BU30.
SOURCEi Search...

Gene expression databases

Bgeei Q8BU30.
CleanExi MM_IARS.
ExpressionAtlasi Q8BU30. baseline and differential.
Genevestigatori Q8BU30.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryo and Thymus.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiSYIC_MOUSE
AccessioniPrimary (citable) accession number: Q8BU30
Secondary accession number(s): Q6NXK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3