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Protein

Isoleucine--tRNA ligase, cytoplasmic

Gene

Iars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei603 – 6031ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: MGI

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: MGI
  2. osteoblast differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IRS
Short name:
IleRS
Gene namesi
Name:Iars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:2145219. Iars.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: GO_Central
  3. extracellular vesicular exosome Source: MGI
  4. membrane Source: MGI
  5. nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12621262Isoleucine--tRNA ligase, cytoplasmicPRO_0000098598Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei1049 – 10491PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ8BU30.
PaxDbiQ8BU30.
PRIDEiQ8BU30.

PTM databases

PhosphoSiteiQ8BU30.

Expressioni

Gene expression databases

BgeeiQ8BU30.
CleanExiMM_IARS.
ExpressionAtlasiQ8BU30. baseline and differential.
GenevestigatoriQ8BU30.

Interactioni

Subunit structurei

Part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.

Protein-protein interaction databases

BioGridi222774. 6 interactions.
IntActiQ8BU30. 1 interaction.
MINTiMINT-1869822.

Structurei

3D structure databases

ProteinModelPortaliQ8BU30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074921.
HOGENOMiHOG000246403.
HOVERGENiHBG013511.
InParanoidiQ8BU30.
KOiK01870.
OMAiNFRALGK.
OrthoDBiEOG7M98FD.
PhylomeDBiQ8BU30.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BU30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQQVPENIS FPAEEEKILE FWSKHNCFQE CLKQSKLRPK FTFYDGPPFA
60 70 80 90 100
TGLPHYGHIL AGTIKDIVTR YAHQSGFHVD RRFGWDCHGL PVEYEIDKTL
110 120 130 140 150
GIKGPEDVAK MGIAEYNKQC RAIVMRYSAE WKSTVTRLGR WIDFDNDYKT
160 170 180 190 200
LYPQFMESVW WVFKQLYDKG LVYRGVKVMP FSTACGTPLS NFESNQNYKD
210 220 230 240 250
VQDPSVFVTF PLEEDENTSL VAWTTTPWTL PSNLALCVNP EIQYVKIKDV
260 270 280 290 300
ARGKLFILTE ARLSALYKQE SDYEILERFP GASLKGKKYK PLFDYFIKCK
310 320 330 340 350
ENGAFTVLVD HYVKDEEGTG VVHQAPYFGA DDHRVCMDFN IIQKDSVPVC
360 370 380 390 400
PVDASGCFTE EVTHFVGQYV KDADKNIIRM LKEQGRLLAA GTFTHSYPFC
410 420 430 440 450
WRSDTPLIYK SVPSWFVRVE PMVDQLLKNN DLCYWVPEFV REKRFGNWLK
460 470 480 490 500
EARDWAISRN RYWGTPIPLW VSEDLEEVVC IGSVAELEEL SGTKISDLHR
510 520 530 540 550
ESIDHLTIPS RCGKAPLRRV SEVFDCWFES GSMPYAQVHY PFESKREFED
560 570 580 590 600
AFPADFIAEG IDQTRGWFYT LLVLATALFG QPPFKNVIVN GLILASDGQK
610 620 630 640 650
MSKRKKNYPD PVSIIDKYGA DALRLYLINS PVVRAENLRF KEEGVRDVLK
660 670 680 690 700
DVLLPWYNAY RFFIQNVFRL HKEEEVKFLY NEHTVRESPN ITDRWVLSFM
710 720 730 740 750
QSLLGFFETE MAAYRLYTVV PRLVKFVDIL TNWYVRMNRR RLKGESGVED
760 770 780 790 800
CVMALETLFS VLLSLCRLMA PYTPFLTELM YQNLKLLIDP ASLRDKDTLS
810 820 830 840 850
IHYLMLPRVR EELIDKKTEN AVSRMQSVIE LGRVIRDRKT IPIKYPLKEI
860 870 880 890 900
VVIHQDPEAL EDIRSLEKYI IEELNVRKVT LSTDKNKYGI RLRAEPDHMV
910 920 930 940 950
LGKRLKGAFK AVMMAIKRLS NEELERFQKS GSIVVEGHEL HEEDIRLMYT
960 970 980 990 1000
FDQATGGTAQ FEAHSDAQAL VLLDVTPDQS MVDEGMAREV INRIQKLRKK
1010 1020 1030 1040 1050
CNLVPTDEIT VYYNAKSEGR YLNNVIESHT DFIFATIKAP LKPYPVPTSD
1060 1070 1080 1090 1100
NILIQEQTQL KGSELEITLT KGSCVPGPAC AYVNLNICAN GTEQGGVLLL
1110 1120 1130 1140 1150
ENPKGDNQLN LVKLKTVVTS VFGVKNAKLS VFHGETEIQN QTDLLSLSGR
1160 1170 1180 1190 1200
TLCVTAGASP SPISSPSTLL CQYLNLQLLN AEPQECLTGT VGTLLLENPL
1210 1220 1230 1240 1250
GQNGLTHQGL VHEAAKVFGL RSRRLRLFLN ETQTQEITED IPMKTLNMKT
1260
VYVSVLPTTA DG
Length:1,262
Mass (Da):144,271
Last modified:July 27, 2011 - v2
Checksum:i2039614F557E1312
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1033 – 10331I → V in BAC40081 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087992 mRNA. Translation: BAC40081.1.
AK133856 mRNA. Translation: BAE21890.1.
CH466546 Genomic DNA. Translation: EDL41088.1.
BC067029 mRNA. Translation: AAH67029.1.
CCDSiCCDS36659.1.
RefSeqiNP_742012.2. NM_172015.3.
XP_006516889.1. XM_006516826.1.
UniGeneiMm.21118.

Genome annotation databases

EnsembliENSMUST00000047363; ENSMUSP00000048096; ENSMUSG00000037851.
ENSMUST00000164260; ENSMUSP00000126806; ENSMUSG00000037851.
ENSMUST00000165316; ENSMUSP00000132082; ENSMUSG00000037851.
GeneIDi105148.
KEGGimmu:105148.
UCSCiuc007qjv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087992 mRNA. Translation: BAC40081.1.
AK133856 mRNA. Translation: BAE21890.1.
CH466546 Genomic DNA. Translation: EDL41088.1.
BC067029 mRNA. Translation: AAH67029.1.
CCDSiCCDS36659.1.
RefSeqiNP_742012.2. NM_172015.3.
XP_006516889.1. XM_006516826.1.
UniGeneiMm.21118.

3D structure databases

ProteinModelPortaliQ8BU30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222774. 6 interactions.
IntActiQ8BU30. 1 interaction.
MINTiMINT-1869822.

PTM databases

PhosphoSiteiQ8BU30.

Proteomic databases

MaxQBiQ8BU30.
PaxDbiQ8BU30.
PRIDEiQ8BU30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047363; ENSMUSP00000048096; ENSMUSG00000037851.
ENSMUST00000164260; ENSMUSP00000126806; ENSMUSG00000037851.
ENSMUST00000165316; ENSMUSP00000132082; ENSMUSG00000037851.
GeneIDi105148.
KEGGimmu:105148.
UCSCiuc007qjv.2. mouse.

Organism-specific databases

CTDi3376.
MGIiMGI:2145219. Iars.

Phylogenomic databases

eggNOGiCOG0060.
GeneTreeiENSGT00550000074921.
HOGENOMiHOG000246403.
HOVERGENiHBG013511.
InParanoidiQ8BU30.
KOiK01870.
OMAiNFRALGK.
OrthoDBiEOG7M98FD.
PhylomeDBiQ8BU30.

Miscellaneous databases

ChiTaRSiIars. mouse.
NextBioi357460.
PROiQ8BU30.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BU30.
CleanExiMM_IARS.
ExpressionAtlasiQ8BU30. baseline and differential.
GenevestigatoriQ8BU30.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Embryo and Thymus.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiSYIC_MOUSE
AccessioniPrimary (citable) accession number: Q8BU30
Secondary accession number(s): Q6NXK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: July 27, 2011
Last modified: March 4, 2015
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.