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Protein

Isoleucine--tRNA ligase, cytoplasmic

Gene

Iars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.1 Publication

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei603ATPBy similarity1

GO - Molecular functioni

  • aminoacyl-tRNA editing activity Source: InterPro
  • ATP binding Source: UniProtKB-KW
  • GTPase binding Source: MGI
  • isoleucine-tRNA ligase activity Source: CAFA
  • tRNA binding Source: InterPro

GO - Biological processi

  • isoleucyl-tRNA aminoacylation Source: CAFA
  • osteoblast differentiation Source: MGI

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.51 Publication)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IRS
Short name:
IleRS
Gene namesi
Name:Iars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2145219. Iars.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985981 – 1262Isoleucine--tRNA ligase, cytoplasmicAdd BLAST1262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei1049PhosphoserineBy similarity1
Modified residuei1058PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BU30.
MaxQBiQ8BU30.
PaxDbiQ8BU30.
PeptideAtlasiQ8BU30.
PRIDEiQ8BU30.

PTM databases

iPTMnetiQ8BU30.
PhosphoSitePlusiQ8BU30.
SwissPalmiQ8BU30.

Expressioni

Gene expression databases

BgeeiENSMUSG00000037851.
CleanExiMM_IARS.
ExpressionAtlasiQ8BU30. baseline and differential.
GenevisibleiQ8BU30. MM.

Interactioni

Subunit structurei

Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi222774. 3 interactors.
IntActiQ8BU30. 1 interactor.
MINTiMINT-1869822.
STRINGi10090.ENSMUSP00000126806.

Structurei

3D structure databases

ProteinModelPortaliQ8BU30.
SMRiQ8BU30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 58"HIGH" regionAdd BLAST11
Motifi600 – 604"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0434. Eukaryota.
COG0060. LUCA.
GeneTreeiENSGT00550000074921.
HOGENOMiHOG000246403.
HOVERGENiHBG013511.
InParanoidiQ8BU30.
KOiK01870.
OMAiKMMAPFT.
OrthoDBiEOG091G01AQ.
PhylomeDBiQ8BU30.

Family and domain databases

CDDicd07961. Anticodon_Ia_Ile_ABEc. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_02003. Ile_tRNA_synth_type2. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033709. Anticodon_Ile_ABEc.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8BU30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQQVPENIS FPAEEEKILE FWSKHNCFQE CLKQSKLRPK FTFYDGPPFA
60 70 80 90 100
TGLPHYGHIL AGTIKDIVTR YAHQSGFHVD RRFGWDCHGL PVEYEIDKTL
110 120 130 140 150
GIKGPEDVAK MGIAEYNKQC RAIVMRYSAE WKSTVTRLGR WIDFDNDYKT
160 170 180 190 200
LYPQFMESVW WVFKQLYDKG LVYRGVKVMP FSTACGTPLS NFESNQNYKD
210 220 230 240 250
VQDPSVFVTF PLEEDENTSL VAWTTTPWTL PSNLALCVNP EIQYVKIKDV
260 270 280 290 300
ARGKLFILTE ARLSALYKQE SDYEILERFP GASLKGKKYK PLFDYFIKCK
310 320 330 340 350
ENGAFTVLVD HYVKDEEGTG VVHQAPYFGA DDHRVCMDFN IIQKDSVPVC
360 370 380 390 400
PVDASGCFTE EVTHFVGQYV KDADKNIIRM LKEQGRLLAA GTFTHSYPFC
410 420 430 440 450
WRSDTPLIYK SVPSWFVRVE PMVDQLLKNN DLCYWVPEFV REKRFGNWLK
460 470 480 490 500
EARDWAISRN RYWGTPIPLW VSEDLEEVVC IGSVAELEEL SGTKISDLHR
510 520 530 540 550
ESIDHLTIPS RCGKAPLRRV SEVFDCWFES GSMPYAQVHY PFESKREFED
560 570 580 590 600
AFPADFIAEG IDQTRGWFYT LLVLATALFG QPPFKNVIVN GLILASDGQK
610 620 630 640 650
MSKRKKNYPD PVSIIDKYGA DALRLYLINS PVVRAENLRF KEEGVRDVLK
660 670 680 690 700
DVLLPWYNAY RFFIQNVFRL HKEEEVKFLY NEHTVRESPN ITDRWVLSFM
710 720 730 740 750
QSLLGFFETE MAAYRLYTVV PRLVKFVDIL TNWYVRMNRR RLKGESGVED
760 770 780 790 800
CVMALETLFS VLLSLCRLMA PYTPFLTELM YQNLKLLIDP ASLRDKDTLS
810 820 830 840 850
IHYLMLPRVR EELIDKKTEN AVSRMQSVIE LGRVIRDRKT IPIKYPLKEI
860 870 880 890 900
VVIHQDPEAL EDIRSLEKYI IEELNVRKVT LSTDKNKYGI RLRAEPDHMV
910 920 930 940 950
LGKRLKGAFK AVMMAIKRLS NEELERFQKS GSIVVEGHEL HEEDIRLMYT
960 970 980 990 1000
FDQATGGTAQ FEAHSDAQAL VLLDVTPDQS MVDEGMAREV INRIQKLRKK
1010 1020 1030 1040 1050
CNLVPTDEIT VYYNAKSEGR YLNNVIESHT DFIFATIKAP LKPYPVPTSD
1060 1070 1080 1090 1100
NILIQEQTQL KGSELEITLT KGSCVPGPAC AYVNLNICAN GTEQGGVLLL
1110 1120 1130 1140 1150
ENPKGDNQLN LVKLKTVVTS VFGVKNAKLS VFHGETEIQN QTDLLSLSGR
1160 1170 1180 1190 1200
TLCVTAGASP SPISSPSTLL CQYLNLQLLN AEPQECLTGT VGTLLLENPL
1210 1220 1230 1240 1250
GQNGLTHQGL VHEAAKVFGL RSRRLRLFLN ETQTQEITED IPMKTLNMKT
1260
VYVSVLPTTA DG
Length:1,262
Mass (Da):144,271
Last modified:July 27, 2011 - v2
Checksum:i2039614F557E1312
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1033I → V in BAC40081 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK087992 mRNA. Translation: BAC40081.1.
AK133856 mRNA. Translation: BAE21890.1.
CH466546 Genomic DNA. Translation: EDL41088.1.
BC067029 mRNA. Translation: AAH67029.1.
CCDSiCCDS36659.1.
RefSeqiNP_742012.2. NM_172015.3.
XP_006516889.1. XM_006516826.2.
UniGeneiMm.21118.

Genome annotation databases

EnsembliENSMUST00000047363; ENSMUSP00000048096; ENSMUSG00000037851.
ENSMUST00000164260; ENSMUSP00000126806; ENSMUSG00000037851.
ENSMUST00000165316; ENSMUSP00000132082; ENSMUSG00000037851.
GeneIDi105148.
KEGGimmu:105148.
UCSCiuc007qjv.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiSYIC_MOUSE
AccessioniPrimary (citable) accession number: Q8BU30
Secondary accession number(s): Q6NXK4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: July 27, 2011
Last modified: September 27, 2017
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families