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Q8BU27 (PPM1M_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein phosphatase 1M

EC=3.1.3.16
Alternative name(s):
Protein phosphatase 2C isoform eta
Short name=PP2C-eta
Short name=PP2CE
Gene names
Name:Ppm1m
Synonyms:Ppm1e
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Subcellular location

Nucleus Ref.1.

Tissue specificity

Widely expressed with highest levels in testis and lower levels in lung, kidney and brain. Ref.1

Sequence similarities

Belongs to the PP2C family.

Contains 1 PP2C-like domain.

Ontologies

Keywords
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionHydrolase
Protein phosphatase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein dephosphorylation

Inferred from direct assay Ref.1. Source: UniProtKB

   Cellular_componentnucleus

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionCTD phosphatase activity

Inferred from direct assay Ref.1. Source: UniProtKB

manganese ion binding

Inferred from direct assay Ref.1. Source: UniProtKB

phosphoprotein phosphatase activity

Inferred from direct assay Ref.1. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q8BU27-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BU27-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406Protein phosphatase 1M
PRO_0000057757

Regions

Domain60 – 299240PP2C-like

Sites

Metal binding711Manganese 1 By similarity UniProtKB P35813
Metal binding711Manganese 2 By similarity
Metal binding721Manganese 1; via carbonyl oxygen By similarity
Metal binding3301Manganese 2 By similarity UniProtKB P35813
Metal binding3871Manganese 2 By similarity

Natural variations

Alternative sequence1 – 100100Missing in isoform 2.
VSP_050658

Experimental info

Sequence conflict1021I → M in AAR01612. Ref.1
Sequence conflict1021I → M in BAC40085. Ref.2
Sequence conflict1561S → L in BAB30649. Ref.2
Sequence conflict2231F → L in BAB30649. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 2, 2004. Version 2.
Checksum: AE82D1B601C2CDF4

FASTA40645,273
        10         20         30         40         50         60 
MYVPPRTSLR VWPMLCGIRV INAEKSEFNE DQAACGKLCI RRCEFGIEED QEWLTVCPEE 

        70         80         90        100        110        120 
FLTGHYWALF DGHGGPAAAI LAANTLHSCL RRQLEAVVEG MIAPQPPMHL SGRCVCPSDP 

       130        140        150        160        170        180 
QFVEEKGIQA EDLVIGALEN AFQECDDVIG RELEASGQVG GCTALVAVFL QGKLYVANAG 

       190        200        210        220        230        240 
DSRAILVRRH EIRQLSSEFT PETERQRIQQ LAFTYPELLA GEFTRLEFPR RLKGDDLGQK 

       250        260        270        280        290        300 
VLFRDHHMRG WSYKRVEKSD LKYPLIHGQG RQARLLGTLA VSRGLGDHQL RVLDTDIQLK 

       310        320        330        340        350        360 
PFLLSIPQVT VLDVHQLAVQ EEDVVVMATD GLWDVLSNEQ VALLVRSFLT GNQKDDPHRF 

       370        380        390        400 
SELAKMLIHN TQGKDNGATG EGQVSYDDVS VFVIPLHSQA QEGSGH 

« Hide

Isoform 2 [UniParc].

Checksum: FA5CDCC368C624FE
Show »

FASTA30634,054

References

« Hide 'large scale' references
[1]"Molecular cloning of PP2Ceta, a novel member of the protein phosphatase 2C family."
Komaki K., Katsura K., Ohnishi M., Li M.G., Sasaki M., Watanabe M., Kobayashi T., Tamura S.
Biochim. Biophys. Acta 1630:130-137(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Embryo.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J and NOD.
Tissue: Corpora quadrigemina, Head and Thymus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY332616 mRNA. Translation: AAR01612.1.
AK013149 mRNA. Translation: BAB28679.2.
AK017245 mRNA. Translation: BAB30649.1.
AK046387 mRNA. Translation: BAC32699.1.
AK087999 mRNA. Translation: BAC40085.1.
CCDSCCDS23471.1. [Q8BU27-1]
RefSeqNP_945149.2. NM_198931.3.
UniGeneMm.23018.

3D structure databases

ProteinModelPortalQ8BU27.
SMRQ8BU27. Positions 62-396.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ8BU27.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID67905.
KEGGmmu:67905.

Organism-specific databases

CTD132160.
MGIMGI:1915155. Ppm1m.

Phylogenomic databases

eggNOGCOG0631.
HOGENOMHOG000251606.
HOVERGENHBG105802.
InParanoidQ8BU27.
KOK17507.
PhylomeDBQ8BU27.

Gene expression databases

CleanExMM_PPM1E.
MM_PPM1M.
GenevestigatorQ8BU27.

Family and domain databases

Gene3D3.60.40.10. 2 hits.
InterProIPR001932. PP2C-like_dom.
IPR015655. Protein_Pase_2C.
[Graphical view]
PANTHERPTHR13832. PTHR13832. 1 hit.
PfamPF00481. PP2C. 2 hits.
[Graphical view]
SMARTSM00331. PP2C_SIG. 1 hit.
SM00332. PP2Cc. 1 hit.
[Graphical view]
SUPFAMSSF81606. SSF81606. 3 hits.
ProtoNetSearch...

Other

NextBio325910.
PROQ8BU27.
SOURCESearch...

Entry information

Entry namePPM1M_MOUSE
AccessionPrimary (citable) accession number: Q8BU27
Secondary accession number(s): Q9CSD6, Q9CU88
Entry history
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: February 2, 2004
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot