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Q8BTY2

- S4A7_MOUSE

UniProt

Q8BTY2 - S4A7_MOUSE

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Protein

Sodium bicarbonate cotransporter 3

Gene
Slc4a7, Nbc3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na+:HCO3- 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity.1 Publication

Enzyme regulationi

Transporter activity is regulated by CA2/carbonic anhydrase 2, cAMP and PKA. Insensitive to stilbene derivatives. May be inhibited by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA) By similarity.

GO - Molecular functioni

  1. inorganic anion exchanger activity Source: InterPro
  2. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. auditory receptor cell development Source: MGI
  2. cochlear nucleus development Source: MGI
  3. retinal cell programmed cell death Source: MGI
  4. retina vasculature morphogenesis in camera-type eye Source: MGI
  5. sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium bicarbonate cotransporter 3
Alternative name(s):
Solute carrier family 4 member 7
Gene namesi
Name:Slc4a7
Synonyms:Nbc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:2443878. Slc4a7.

Subcellular locationi

Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein By similarity. Cell projectionstereocilium By similarity
Note: Also described at the apical cell membrane. Localizes to the stereocilia of cochlear outer hair cells and to the lateral membrane of cochlear inner hair cells By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 476476Extracellular Reviewed predictionAdd
BLAST
Transmembranei477 – 49721Helical; Reviewed predictionAdd
BLAST
Topological domaini498 – 5058Cytoplasmic Reviewed prediction
Transmembranei506 – 52621Helical; Reviewed predictionAdd
BLAST
Topological domaini527 – 56337Extracellular Reviewed predictionAdd
BLAST
Transmembranei564 – 58421Helical; Reviewed predictionAdd
BLAST
Topological domaini585 – 5939Cytoplasmic Reviewed prediction
Transmembranei594 – 61421Helical; Reviewed predictionAdd
BLAST
Topological domaini615 – 68571Extracellular Reviewed predictionAdd
BLAST
Transmembranei686 – 70621Helical; Reviewed predictionAdd
BLAST
Topological domaini707 – 72923Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei730 – 75021Helical; Reviewed predictionAdd
BLAST
Topological domaini751 – 77626Extracellular Reviewed predictionAdd
BLAST
Transmembranei777 – 79721Helical; Reviewed predictionAdd
BLAST
Topological domaini798 – 81215Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei813 – 83321Helical; Reviewed predictionAdd
BLAST
Topological domaini834 – 87643Extracellular Reviewed predictionAdd
BLAST
Transmembranei877 – 89721Helical; Reviewed predictionAdd
BLAST
Topological domaini898 – 1034137Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. basolateral plasma membrane Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. integral component of membrane Source: UniProtKB-KW
  5. stereocilium Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

Deafness and blindness due to degeneration of sensory receptors in internal ear and retina.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10341034Sodium bicarbonate cotransporter 3PRO_0000079234Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571Phosphoserine1 Publication
Glycosylationi176 – 1761N-linked (GlcNAc...) Reviewed prediction
Modified residuei238 – 2381Phosphoserine1 Publication
Modified residuei271 – 2711Phosphoserine By similarity
Glycosylationi274 – 2741N-linked (GlcNAc...) Reviewed prediction
Modified residuei275 – 2751Phosphoserine1 Publication
Glycosylationi644 – 6441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi654 – 6541N-linked (GlcNAc...)1 Publication
Glycosylationi664 – 6641N-linked (GlcNAc...)1 Publication
Modified residuei951 – 9511Phosphothreonine By similarity
Modified residuei960 – 9601Phosphoserine2 Publications
Modified residuei1033 – 10331Phosphoserine By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8BTY2.
PaxDbiQ8BTY2.
PRIDEiQ8BTY2.

PTM databases

PhosphoSiteiQ8BTY2.

Expressioni

Tissue specificityi

Expressed in the spiral ligament throughout the cochlea and in photoreceptors of the outer plexiform layer of the retina (at protein level).2 Publications

Gene expression databases

GenevestigatoriQ8BTY2.

Interactioni

Subunit structurei

Forms a complex with ATP6V1B1 and SLC9A3R1/EBP50. Interacts in a pH dependent-manner with CA2/carbonic anhydrase 2 By similarity. Interacts with CFTR through SLC9A3R1/EBP50. Interacts with USH1C.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ8BTY2.
SMRiQ8BTY2. Positions 115-394, 460-500.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni918 – 9203CA2-binding By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1031 – 10344PDZ-binding By similarity

Domaini

The PDZ-binding motif mediates interaction with the CFTR, SLC9A3R1/EBP50 complex and probably with USH1C By similarity.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG268067.
HOGENOMiHOG000280684.
HOVERGENiHBG004326.
PhylomeDBiQ8BTY2.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BTY2-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEADGAGEQM RPLLTRGPDE EAVVDLGKTS STVNTKFEKE ELESHRAVYV     50
GVHVPFSKES RRRHKHRGHK HHHRRRKDKD SDKEDGRESP SYDTPSQRVQ 100
FILGTEDDDE EHIPHDLFTE MDELCYRDGE EYEWKETARW LKFEEDVEDG 150
GDRWSKPYVA TLSLHSLFEL RSCILNGTVM LDMRASTLDE IADMVLDNMI 200
ASGQLDDSIR ENVREALLKR HHHQNEKRFT SRIPLVRSFA DIGILASPQS 250
APGNLDNSKS GEMKGNGSGG SRENSTVDFS KVDMNFMRKI PTGAEASNVL 300
VGEVDFLERP IIAFVRLAPA VLLSGLTEVP VPTRFLFLLL GPAGKAPQYH 350
EIGRSIATLM TDEIFHDVAY KAKDRNDLLS GIDEFLDQVT VLPPGEWDPS 400
IRIEPPKSVP SQEKRKIPVF PNGSAAMSVG PPKEDDHHAG PELQRTGRLF 450
GGLILDIKRK APFFLSDFKD ALSLQCLASI LFLYCACMSP VITFGGLLGE 500
ATEGRISAIE SLFGASLTGI AYSLFAGQPL TILGSTGPVL VFEKILFKFC 550
RDYHLSYLSL RTSIGLWTSF LCIVLVATDA SSLVCYITRF TEEAFAALIC 600
IIFIYEALEK LFHLGEIYAF NMHNNLDELT SYTCVCAEPS NPSNETLELW 650
KRKNITAYSV SWGNLTVSEC KTFHGMFVGS ACGPHGPYVP DVLFWCVVLF 700
FTTFFLSSFL KQFKTKGYFP TKVRSTISDF AVFLTIVIMV AIDYLVGIPS 750
PKLHVPEKFE PTDPSRGWII SPLGDNPWWT LLIAAVPALL CTILIFMDQQ 800
ITAVIINRKE HKLKFIPMPV LYGVFLYMGV SSLKGIQFFD RIKLFGMPAK 850
HQPDLIYLRY VPLWKVHVFT VVQLTCLVLL WVIKASAAAV VFPMMVLALV 900
FVRKLMDLCF TKRELSWLDD LMPESKKKKE DDKKKKEKEE AERMLQDDED 950
TVHLPFERGS LLQIPVKTLK YSIDPSVVNI SDEMAKTAQW KALSMNTENA 1000
KVTRPNTSPE KPVSVTINFE DEPSKKYMDA ETSL 1034
Length:1,034
Mass (Da):116,514
Last modified:February 7, 2006 - v2
Checksum:i92B080274D206097
GO
Isoform 2 (identifier: Q8BTY2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     243-243: G → GKKHSDPHLLERNG
     815-815: F → KGAGYHLDLL...FILSCKEIKH
     816-1034: Missing.

Note: Contains a phosphoserine at position 263.

Show »
Length:936
Mass (Da):104,678
Checksum:i03664D0534FD2EBC
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei243 – 2431G → GKKHSDPHLLERNG in isoform 2. VSP_017166
Alternative sequencei815 – 8151F → KGAGYHLDLLMVGVMLGVCS IMGLPWFVAATVLSISHVNS LKVESECSAPGEQPKFLGIR EQRVTGLMIFILMGLSVFMT SVLKVKPLWQCSTILFTLTF ILSCKEIKH in isoform 2. VSP_017167
Alternative sequencei816 – 1034219Missing in isoform 2. VSP_017168Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti430 – 4301G → D in BAC40330. 1 Publication
Sequence conflicti717 – 7171G → R in BAC40330. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK088400 mRNA. Translation: BAC40330.1.
AK152233 mRNA. Translation: BAE31059.1.
AK153567 mRNA. Translation: BAE32101.1.
CN532915 mRNA. No translation available.
UniGeneiMm.258893.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK088400 mRNA. Translation: BAC40330.1 .
AK152233 mRNA. Translation: BAE31059.1 .
AK153567 mRNA. Translation: BAE32101.1 .
CN532915 mRNA. No translation available.
UniGenei Mm.258893.

3D structure databases

ProteinModelPortali Q8BTY2.
SMRi Q8BTY2. Positions 115-394, 460-500.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8BTY2.

Proteomic databases

MaxQBi Q8BTY2.
PaxDbi Q8BTY2.
PRIDEi Q8BTY2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:2443878. Slc4a7.

Phylogenomic databases

eggNOGi NOG268067.
HOGENOMi HOG000280684.
HOVERGENi HBG004326.
PhylomeDBi Q8BTY2.

Miscellaneous databases

ChiTaRSi SLC4A7. mouse.
PROi Q8BTY2.
SOURCEi Search...

Gene expression databases

Genevestigatori Q8BTY2.

Family and domain databases

Gene3Di 3.40.1100.10. 1 hit.
InterProi IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view ]
PANTHERi PTHR11453. PTHR11453. 1 hit.
Pfami PF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view ]
PRINTSi PR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMi SSF55804. SSF55804. 1 hit.
TIGRFAMsi TIGR00834. ae. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-932 (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 826-1034 (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Brain.
  3. "The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3-cotransport isoform 3."
    Park M., Ko S.B.H., Choi J.Y., Muallem G., Thomas P.J., Pushkin A., Lee M.-S., Kim J.Y., Lee M.G., Muallem S., Kurtz I.
    J. Biol. Chem. 277:50503-50509(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CFTR AND SLC9A3R1.
  4. Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  5. "Scaffold protein harmonin (USH1C) provides molecular links between Usher syndrome type 1 and type 2."
    Reiners J., van Wijk E., Maerker T., Zimmermann U., Juergens K., te Brinke H., Overlack N., Roepman R., Knipper M., Kremer H., Wolfrum U.
    Hum. Mol. Genet. 14:3933-3943(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH USH1C, TISSUE SPECIFICITY.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57; SER-238 AND SER-960, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-960, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  9. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-654 AND ASN-664.

Entry informationi

Entry nameiS4A7_MOUSE
AccessioniPrimary (citable) accession number: Q8BTY2
Secondary accession number(s): Q3U5H7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: June 11, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi