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Protein

Sodium bicarbonate cotransporter 3

Gene

Slc4a7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na+:HCO3- 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity.1 Publication

Enzyme regulationi

Transporter activity is regulated by CA2/carbonic anhydrase 2, cAMP and PKA. Insensitive to stilbene derivatives. May be inhibited by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA) (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • anion transmembrane transport Source: GO_Central
  • auditory receptor cell development Source: MGI
  • bicarbonate transport Source: GO_Central
  • camera-type eye photoreceptor cell differentiation Source: MGI
  • cochlear nucleus development Source: MGI
  • regulation of intracellular pH Source: GO_Central
  • retinal cell programmed cell death Source: MGI
  • retina vasculature morphogenesis in camera-type eye Source: MGI
  • sodium ion transport Source: UniProtKB-KW
  • visual perception Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium bicarbonate cotransporter 3
Alternative name(s):
Solute carrier family 4 member 7
Gene namesi
Name:Slc4a7
Synonyms:Nbc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2443878. Slc4a7.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 476ExtracellularSequence analysisAdd BLAST476
Transmembranei477 – 497HelicalSequence analysisAdd BLAST21
Topological domaini498 – 505CytoplasmicSequence analysis8
Transmembranei506 – 526HelicalSequence analysisAdd BLAST21
Topological domaini527 – 563ExtracellularSequence analysisAdd BLAST37
Transmembranei564 – 584HelicalSequence analysisAdd BLAST21
Topological domaini585 – 593CytoplasmicSequence analysis9
Transmembranei594 – 614HelicalSequence analysisAdd BLAST21
Topological domaini615 – 685ExtracellularSequence analysisAdd BLAST71
Transmembranei686 – 706HelicalSequence analysisAdd BLAST21
Topological domaini707 – 729CytoplasmicSequence analysisAdd BLAST23
Transmembranei730 – 750HelicalSequence analysisAdd BLAST21
Topological domaini751 – 776ExtracellularSequence analysisAdd BLAST26
Transmembranei777 – 797HelicalSequence analysisAdd BLAST21
Topological domaini798 – 812CytoplasmicSequence analysisAdd BLAST15
Transmembranei813 – 833HelicalSequence analysisAdd BLAST21
Topological domaini834 – 876ExtracellularSequence analysisAdd BLAST43
Transmembranei877 – 897HelicalSequence analysisAdd BLAST21
Topological domaini898 – 1034CytoplasmicSequence analysisAdd BLAST137

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • basolateral plasma membrane Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: MGI
  • stereocilium Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

Pathology & Biotechi

Disruption phenotypei

Deafness and blindness due to degeneration of sensory receptors in internal ear and retina.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000792341 – 1034Sodium bicarbonate cotransporter 3Add BLAST1034

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57PhosphoserineCombined sources1
Modified residuei60PhosphoserineBy similarity1
Modified residuei89PhosphoserineBy similarity1
Modified residuei155PhosphoserineBy similarity1
Glycosylationi176N-linked (GlcNAc...)Sequence analysis1
Modified residuei238PhosphoserineCombined sources1
Modified residuei250PhosphoserineCombined sources1
Modified residuei268PhosphoserineBy similarity1
Modified residuei271PhosphoserineCombined sources1
Glycosylationi274N-linked (GlcNAc...)Sequence analysis1
Modified residuei275PhosphoserineCombined sources1
Modified residuei424PhosphoserineBy similarity1
Disulfide bondi634 ↔ 636By similarity
Glycosylationi644N-linked (GlcNAc...)Sequence analysis1
Glycosylationi654N-linked (GlcNAc...)1 Publication1
Glycosylationi664N-linked (GlcNAc...)1 Publication1
Disulfide bondi670 ↔ 682By similarity
Modified residuei951PhosphothreonineCombined sources1
Modified residuei960PhosphoserineCombined sources1
Modified residuei1033PhosphoserineBy similarity1
Isoform 2 (identifier: Q8BTY2-2)
Modified residuei260PhosphoserineCombined sources1
Modified residuei263PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8BTY2.
PaxDbiQ8BTY2.
PeptideAtlasiQ8BTY2.
PRIDEiQ8BTY2.

PTM databases

iPTMnetiQ8BTY2.
PhosphoSitePlusiQ8BTY2.
SwissPalmiQ8BTY2.

Expressioni

Tissue specificityi

Expressed in the spiral ligament throughout the cochlea and in photoreceptors of the outer plexiform layer of the retina (at protein level).2 Publications

Interactioni

Subunit structurei

Forms a complex with ATP6V1B1 and SLC9A3R1/EBP50. Interacts in a pH dependent-manner with CA2/carbonic anhydrase 2 (By similarity). Interacts with CFTR through SLC9A3R1/EBP50. Interacts with USH1C.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ush1cQ9ES64-32EBI-11621670,EBI-7418919

Protein-protein interaction databases

IntActiQ8BTY2. 1 interactor.
STRINGi10090.ENSMUSP00000058313.

Structurei

3D structure databases

ProteinModelPortaliQ8BTY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni918 – 920CA2-bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1031 – 1034PDZ-bindingBy similarity4

Domaini

The PDZ-binding motif mediates interaction with the CFTR, SLC9A3R1/EBP50 complex and probably with USH1C.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
HOGENOMiHOG000280684.
HOVERGENiHBG004326.
InParanoidiQ8BTY2.
PhylomeDBiQ8BTY2.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BTY2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEADGAGEQM RPLLTRGPDE EAVVDLGKTS STVNTKFEKE ELESHRAVYV
60 70 80 90 100
GVHVPFSKES RRRHKHRGHK HHHRRRKDKD SDKEDGRESP SYDTPSQRVQ
110 120 130 140 150
FILGTEDDDE EHIPHDLFTE MDELCYRDGE EYEWKETARW LKFEEDVEDG
160 170 180 190 200
GDRWSKPYVA TLSLHSLFEL RSCILNGTVM LDMRASTLDE IADMVLDNMI
210 220 230 240 250
ASGQLDDSIR ENVREALLKR HHHQNEKRFT SRIPLVRSFA DIGILASPQS
260 270 280 290 300
APGNLDNSKS GEMKGNGSGG SRENSTVDFS KVDMNFMRKI PTGAEASNVL
310 320 330 340 350
VGEVDFLERP IIAFVRLAPA VLLSGLTEVP VPTRFLFLLL GPAGKAPQYH
360 370 380 390 400
EIGRSIATLM TDEIFHDVAY KAKDRNDLLS GIDEFLDQVT VLPPGEWDPS
410 420 430 440 450
IRIEPPKSVP SQEKRKIPVF PNGSAAMSVG PPKEDDHHAG PELQRTGRLF
460 470 480 490 500
GGLILDIKRK APFFLSDFKD ALSLQCLASI LFLYCACMSP VITFGGLLGE
510 520 530 540 550
ATEGRISAIE SLFGASLTGI AYSLFAGQPL TILGSTGPVL VFEKILFKFC
560 570 580 590 600
RDYHLSYLSL RTSIGLWTSF LCIVLVATDA SSLVCYITRF TEEAFAALIC
610 620 630 640 650
IIFIYEALEK LFHLGEIYAF NMHNNLDELT SYTCVCAEPS NPSNETLELW
660 670 680 690 700
KRKNITAYSV SWGNLTVSEC KTFHGMFVGS ACGPHGPYVP DVLFWCVVLF
710 720 730 740 750
FTTFFLSSFL KQFKTKGYFP TKVRSTISDF AVFLTIVIMV AIDYLVGIPS
760 770 780 790 800
PKLHVPEKFE PTDPSRGWII SPLGDNPWWT LLIAAVPALL CTILIFMDQQ
810 820 830 840 850
ITAVIINRKE HKLKFIPMPV LYGVFLYMGV SSLKGIQFFD RIKLFGMPAK
860 870 880 890 900
HQPDLIYLRY VPLWKVHVFT VVQLTCLVLL WVIKASAAAV VFPMMVLALV
910 920 930 940 950
FVRKLMDLCF TKRELSWLDD LMPESKKKKE DDKKKKEKEE AERMLQDDED
960 970 980 990 1000
TVHLPFERGS LLQIPVKTLK YSIDPSVVNI SDEMAKTAQW KALSMNTENA
1010 1020 1030
KVTRPNTSPE KPVSVTINFE DEPSKKYMDA ETSL
Length:1,034
Mass (Da):116,514
Last modified:February 7, 2006 - v2
Checksum:i92B080274D206097
GO
Isoform 2 (identifier: Q8BTY2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     243-243: G → GKKHSDPHLLERNG
     815-815: F → KGAGYHLDLL...FILSCKEIKH
     816-1034: Missing.

Show »
Length:936
Mass (Da):104,678
Checksum:i03664D0534FD2EBC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti430G → D in BAC40330 (PubMed:16141072).Curated1
Sequence conflicti717G → R in BAC40330 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017166243G → GKKHSDPHLLERNG in isoform 2. 1 Publication1
Alternative sequenceiVSP_017167815F → KGAGYHLDLLMVGVMLGVCS IMGLPWFVAATVLSISHVNS LKVESECSAPGEQPKFLGIR EQRVTGLMIFILMGLSVFMT SVLKVKPLWQCSTILFTLTF ILSCKEIKH in isoform 2. 1 Publication1
Alternative sequenceiVSP_017168816 – 1034Missing in isoform 2. 1 PublicationAdd BLAST219

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK088400 mRNA. Translation: BAC40330.1.
AK152233 mRNA. Translation: BAE31059.1.
AK153567 mRNA. Translation: BAE32101.1.
CN532915 mRNA. No translation available.
UniGeneiMm.258893.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK088400 mRNA. Translation: BAC40330.1.
AK152233 mRNA. Translation: BAE31059.1.
AK153567 mRNA. Translation: BAE32101.1.
CN532915 mRNA. No translation available.
UniGeneiMm.258893.

3D structure databases

ProteinModelPortaliQ8BTY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BTY2. 1 interactor.
STRINGi10090.ENSMUSP00000058313.

PTM databases

iPTMnetiQ8BTY2.
PhosphoSitePlusiQ8BTY2.
SwissPalmiQ8BTY2.

Proteomic databases

EPDiQ8BTY2.
PaxDbiQ8BTY2.
PeptideAtlasiQ8BTY2.
PRIDEiQ8BTY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:2443878. Slc4a7.

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
HOGENOMiHOG000280684.
HOVERGENiHBG004326.
InParanoidiQ8BTY2.
PhylomeDBiQ8BTY2.

Miscellaneous databases

ChiTaRSiSlc4a7. mouse.
PROiQ8BTY2.
SOURCEiSearch...

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS4A7_MOUSE
AccessioniPrimary (citable) accession number: Q8BTY2
Secondary accession number(s): Q3U5H7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: February 7, 2006
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.