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Q8BTY2

- S4A7_MOUSE

UniProt

Q8BTY2 - S4A7_MOUSE

Protein

Sodium bicarbonate cotransporter 3

Gene

Slc4a7

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (07 Feb 2006)
      Previous versions | rss
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    Functioni

    Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na+:HCO3- 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity.1 Publication

    Enzyme regulationi

    Transporter activity is regulated by CA2/carbonic anhydrase 2, cAMP and PKA. Insensitive to stilbene derivatives. May be inhibited by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA) By similarity.By similarity

    GO - Molecular functioni

    1. inorganic anion exchanger activity Source: InterPro
    2. symporter activity Source: UniProtKB-KW

    GO - Biological processi

    1. auditory receptor cell development Source: MGI
    2. cochlear nucleus development Source: MGI
    3. retinal cell programmed cell death Source: MGI
    4. retina vasculature morphogenesis in camera-type eye Source: MGI
    5. sodium ion transport Source: UniProtKB-KW

    Keywords - Biological processi

    Ion transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium bicarbonate cotransporter 3
    Alternative name(s):
    Solute carrier family 4 member 7
    Gene namesi
    Name:Slc4a7
    Synonyms:Nbc3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:2443878. Slc4a7.

    Subcellular locationi

    Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane By similarity; Multi-pass membrane protein By similarity. Cell projectionstereocilium By similarity
    Note: Also described at the apical cell membrane. Localizes to the stereocilia of cochlear outer hair cells and to the lateral membrane of cochlear inner hair cells By similarity.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. basolateral plasma membrane Source: UniProtKB
    3. cytoplasm Source: UniProtKB
    4. integral component of membrane Source: UniProtKB-KW
    5. stereocilium Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Deafness and blindness due to degeneration of sensory receptors in internal ear and retina.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10341034Sodium bicarbonate cotransporter 3PRO_0000079234Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei57 – 571Phosphoserine1 Publication
    Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
    Modified residuei238 – 2381Phosphoserine1 Publication
    Modified residuei271 – 2711PhosphoserineBy similarity
    Glycosylationi274 – 2741N-linked (GlcNAc...)Sequence Analysis
    Modified residuei275 – 2751Phosphoserine1 Publication
    Glycosylationi644 – 6441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi654 – 6541N-linked (GlcNAc...)1 Publication
    Glycosylationi664 – 6641N-linked (GlcNAc...)1 Publication
    Modified residuei951 – 9511PhosphothreonineBy similarity
    Modified residuei960 – 9601Phosphoserine2 Publications
    Modified residuei1033 – 10331PhosphoserineBy similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiQ8BTY2.
    PaxDbiQ8BTY2.
    PRIDEiQ8BTY2.

    PTM databases

    PhosphoSiteiQ8BTY2.

    Expressioni

    Tissue specificityi

    Expressed in the spiral ligament throughout the cochlea and in photoreceptors of the outer plexiform layer of the retina (at protein level).2 Publications

    Gene expression databases

    GenevestigatoriQ8BTY2.

    Interactioni

    Subunit structurei

    Forms a complex with ATP6V1B1 and SLC9A3R1/EBP50. Interacts in a pH dependent-manner with CA2/carbonic anhydrase 2 By similarity. Interacts with CFTR through SLC9A3R1/EBP50. Interacts with USH1C.By similarity2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BTY2.
    SMRiQ8BTY2. Positions 115-394, 460-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 476476ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini498 – 5058CytoplasmicSequence Analysis
    Topological domaini527 – 56337ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini585 – 5939CytoplasmicSequence Analysis
    Topological domaini615 – 68571ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini707 – 72923CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini751 – 77626ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini798 – 81215CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini834 – 87643ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini898 – 1034137CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei477 – 49721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei506 – 52621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei564 – 58421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei594 – 61421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei686 – 70621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei730 – 75021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei777 – 79721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei813 – 83321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei877 – 89721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni918 – 9203CA2-bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1031 – 10344PDZ-bindingBy similarity

    Domaini

    The PDZ-binding motif mediates interaction with the CFTR, SLC9A3R1/EBP50 complex and probably with USH1C.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG268067.
    HOGENOMiHOG000280684.
    HOVERGENiHBG004326.
    PhylomeDBiQ8BTY2.

    Family and domain databases

    Gene3Di3.40.1100.10. 1 hit.
    InterProiIPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR003024. Na/HCO3_transpt.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view]
    PANTHERiPTHR11453. PTHR11453. 1 hit.
    PfamiPF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 1 hit.
    [Graphical view]
    PRINTSiPR01231. HCO3TRNSPORT.
    PR01232. NAHCO3TRSPRT.
    SUPFAMiSSF55804. SSF55804. 1 hit.
    TIGRFAMsiTIGR00834. ae. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BTY2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEADGAGEQM RPLLTRGPDE EAVVDLGKTS STVNTKFEKE ELESHRAVYV     50
    GVHVPFSKES RRRHKHRGHK HHHRRRKDKD SDKEDGRESP SYDTPSQRVQ 100
    FILGTEDDDE EHIPHDLFTE MDELCYRDGE EYEWKETARW LKFEEDVEDG 150
    GDRWSKPYVA TLSLHSLFEL RSCILNGTVM LDMRASTLDE IADMVLDNMI 200
    ASGQLDDSIR ENVREALLKR HHHQNEKRFT SRIPLVRSFA DIGILASPQS 250
    APGNLDNSKS GEMKGNGSGG SRENSTVDFS KVDMNFMRKI PTGAEASNVL 300
    VGEVDFLERP IIAFVRLAPA VLLSGLTEVP VPTRFLFLLL GPAGKAPQYH 350
    EIGRSIATLM TDEIFHDVAY KAKDRNDLLS GIDEFLDQVT VLPPGEWDPS 400
    IRIEPPKSVP SQEKRKIPVF PNGSAAMSVG PPKEDDHHAG PELQRTGRLF 450
    GGLILDIKRK APFFLSDFKD ALSLQCLASI LFLYCACMSP VITFGGLLGE 500
    ATEGRISAIE SLFGASLTGI AYSLFAGQPL TILGSTGPVL VFEKILFKFC 550
    RDYHLSYLSL RTSIGLWTSF LCIVLVATDA SSLVCYITRF TEEAFAALIC 600
    IIFIYEALEK LFHLGEIYAF NMHNNLDELT SYTCVCAEPS NPSNETLELW 650
    KRKNITAYSV SWGNLTVSEC KTFHGMFVGS ACGPHGPYVP DVLFWCVVLF 700
    FTTFFLSSFL KQFKTKGYFP TKVRSTISDF AVFLTIVIMV AIDYLVGIPS 750
    PKLHVPEKFE PTDPSRGWII SPLGDNPWWT LLIAAVPALL CTILIFMDQQ 800
    ITAVIINRKE HKLKFIPMPV LYGVFLYMGV SSLKGIQFFD RIKLFGMPAK 850
    HQPDLIYLRY VPLWKVHVFT VVQLTCLVLL WVIKASAAAV VFPMMVLALV 900
    FVRKLMDLCF TKRELSWLDD LMPESKKKKE DDKKKKEKEE AERMLQDDED 950
    TVHLPFERGS LLQIPVKTLK YSIDPSVVNI SDEMAKTAQW KALSMNTENA 1000
    KVTRPNTSPE KPVSVTINFE DEPSKKYMDA ETSL 1034
    Length:1,034
    Mass (Da):116,514
    Last modified:February 7, 2006 - v2
    Checksum:i92B080274D206097
    GO
    Isoform 2 (identifier: Q8BTY2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         243-243: G → GKKHSDPHLLERNG
         815-815: F → KGAGYHLDLL...FILSCKEIKH
         816-1034: Missing.

    Note: Contains a phosphoserine at position 263.

    Show »
    Length:936
    Mass (Da):104,678
    Checksum:i03664D0534FD2EBC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti430 – 4301G → D in BAC40330. (PubMed:16141072)Curated
    Sequence conflicti717 – 7171G → R in BAC40330. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei243 – 2431G → GKKHSDPHLLERNG in isoform 2. 1 PublicationVSP_017166
    Alternative sequencei815 – 8151F → KGAGYHLDLLMVGVMLGVCS IMGLPWFVAATVLSISHVNS LKVESECSAPGEQPKFLGIR EQRVTGLMIFILMGLSVFMT SVLKVKPLWQCSTILFTLTF ILSCKEIKH in isoform 2. 1 PublicationVSP_017167
    Alternative sequencei816 – 1034219Missing in isoform 2. 1 PublicationVSP_017168Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK088400 mRNA. Translation: BAC40330.1.
    AK152233 mRNA. Translation: BAE31059.1.
    AK153567 mRNA. Translation: BAE32101.1.
    CN532915 mRNA. No translation available.
    UniGeneiMm.258893.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK088400 mRNA. Translation: BAC40330.1 .
    AK152233 mRNA. Translation: BAE31059.1 .
    AK153567 mRNA. Translation: BAE32101.1 .
    CN532915 mRNA. No translation available.
    UniGenei Mm.258893.

    3D structure databases

    ProteinModelPortali Q8BTY2.
    SMRi Q8BTY2. Positions 115-394, 460-500.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BTY2.

    Proteomic databases

    MaxQBi Q8BTY2.
    PaxDbi Q8BTY2.
    PRIDEi Q8BTY2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:2443878. Slc4a7.

    Phylogenomic databases

    eggNOGi NOG268067.
    HOGENOMi HOG000280684.
    HOVERGENi HBG004326.
    PhylomeDBi Q8BTY2.

    Miscellaneous databases

    ChiTaRSi SLC4A7. mouse.
    PROi Q8BTY2.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q8BTY2.

    Family and domain databases

    Gene3Di 3.40.1100.10. 1 hit.
    InterProi IPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR003024. Na/HCO3_transpt.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view ]
    PANTHERi PTHR11453. PTHR11453. 1 hit.
    Pfami PF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 1 hit.
    [Graphical view ]
    PRINTSi PR01231. HCO3TRNSPORT.
    PR01232. NAHCO3TRSPRT.
    SUPFAMi SSF55804. SSF55804. 1 hit.
    TIGRFAMsi TIGR00834. ae. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-932 (ISOFORM 1).
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 826-1034 (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    3. "The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3-cotransport isoform 3."
      Park M., Ko S.B.H., Choi J.Y., Muallem G., Thomas P.J., Pushkin A., Lee M.-S., Kim J.Y., Lee M.G., Muallem S., Kurtz I.
      J. Biol. Chem. 277:50503-50509(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CFTR AND SLC9A3R1.
    4. Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    5. "Scaffold protein harmonin (USH1C) provides molecular links between Usher syndrome type 1 and type 2."
      Reiners J., van Wijk E., Maerker T., Zimmermann U., Juergens K., te Brinke H., Overlack N., Roepman R., Knipper M., Kremer H., Wolfrum U.
      Hum. Mol. Genet. 14:3933-3943(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH USH1C, TISSUE SPECIFICITY.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-275, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57; SER-238 AND SER-960, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-263 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-960, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    9. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-654 AND ASN-664.

    Entry informationi

    Entry nameiS4A7_MOUSE
    AccessioniPrimary (citable) accession number: Q8BTY2
    Secondary accession number(s): Q3U5H7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: February 7, 2006
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3