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Protein

Tumor suppressor candidate 3

Gene

Tusc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as accessory component of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Involved in N-glycosylation of STT3B-dependent substrates. Specifically required for the glycosylation of a subset of acceptor sites that are near cysteine residues; in this function seems to act redundantly with MAGT1. In its oxidized form proposed to form transient mixed disulfides with a glycoprotein substrate to facilitate access of STT3B to the unmodified acceptor site. Has also oxidoreductase-independent functions in the STT3B-containing OST complex possibly involving substrate recognition.By similarity
Magnesium transporter.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTransport
LigandMagnesium

Enzyme and pathway databases

ReactomeiR-MMU-5223345 Miscellaneous transport and binding events
UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Tumor suppressor candidate 3
Alternative name(s):
Magnesium uptake/transporter TUSC3
Protein N33
Gene namesi
Name:Tusc3
Synonyms:N33
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1933134 Tusc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini42 – 196LumenalSequence analysisAdd BLAST155
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Topological domaini218 – 221CytoplasmicSequence analysis4
Transmembranei222 – 242HelicalSequence analysisAdd BLAST21
Topological domaini243 – 276LumenalSequence analysisAdd BLAST34
Transmembranei277 – 297HelicalSequence analysisAdd BLAST21
Topological domaini298 – 312CytoplasmicSequence analysisAdd BLAST15
Transmembranei313 – 333HelicalSequence analysisAdd BLAST21
Topological domaini334 – 347LumenalSequence analysisAdd BLAST14

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 41Sequence analysisAdd BLAST41
ChainiPRO_000041492342 – 347Tumor suppressor candidate 3Add BLAST306

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi99 ↔ 102Redox-activeBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8BTV1
PaxDbiQ8BTV1
PeptideAtlasiQ8BTV1
PRIDEiQ8BTV1

PTM databases

iPTMnetiQ8BTV1
PhosphoSitePlusiQ8BTV1

Expressioni

Gene expression databases

BgeeiENSMUSG00000039530
ExpressionAtlasiQ8BTV1 baseline and differential
GenevisibleiQ8BTV1 MM

Interactioni

Subunit structurei

Accessory component of the STT3B-containing form of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. The association of TUSC3 or MAGT1 with the STT3B-containing complex seems to be mutually exclusvice.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000126080

Structurei

3D structure databases

ProteinModelPortaliQ8BTV1
SMRiQ8BTV1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 187ThioredoxinAdd BLAST129

Sequence similaritiesi

Belongs to the OST3/OST6 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2603 Eukaryota
ENOG410XR1F LUCA
GeneTreeiENSGT00390000012030
HOGENOMiHOG000231301
HOVERGENiHBG002493
InParanoidiQ8BTV1
KOiK12669
OMAiWIGERTD
OrthoDBiEOG091G0ERM
PhylomeDBiQ8BTV1
TreeFamiTF314850

Family and domain databases

InterProiView protein in InterPro
IPR021149 OligosaccharylTrfase_OST3/OST6
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF04756 OST3_OST6, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BTV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSARAAPSRR RQAGRRLRYL PTGSFPFLLL LLLLCIQLGG GQKKKENLLA
60 70 80 90 100
EKVEQLMEWS SRRSIFRMNG DKFRKFVKAP PRNYSMIVMF TALQPQRQCS
110 120 130 140 150
VCRQANEEYQ ILANSWRYSS AFCNKLFFGM VDYDEGTDVF QQLNMNSAPT
160 170 180 190 200
FMHFPSKGRP KRADTFDLQR IGFAAEQLAK WIADRTDVHI RVFRPPNYSG
210 220 230 240 250
TIALALLVSL VGGLLYLRRN NLEFIYNKTG WAMVSLCIVF AMTSGQMWNH
260 270 280 290 300
IRGPPYAHKN PHNGQVSYIH GSSQAQFVAE SHIILVLNAA ITMGMVLLNE
310 320 330 340
AATSKGDVGK RRIICLVGLG LVVFFFSFLL SIFRSKYHGY PYSFLIK
Length:347
Mass (Da):39,548
Last modified:March 1, 2003 - v1
Checksum:iA2C6A79FA2A979C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK088616 mRNA Translation: BAC40455.1
AK132766 mRNA Translation: BAE21346.1
AC168314 Genomic DNA No translation available.
AC174643 Genomic DNA No translation available.
CAAA01027656 Genomic DNA No translation available.
BC069898 mRNA Translation: AAH69898.1
CCDSiCCDS85529.1
RefSeqiNP_084530.3, NM_030254.3
UniGeneiMm.272722
Mm.432012

Genome annotation databases

EnsembliENSMUST00000167992; ENSMUSP00000126080; ENSMUSG00000039530
ENSMUST00000209440; ENSMUSP00000148134; ENSMUSG00000039530
GeneIDi80286
KEGGimmu:80286
UCSCiuc009lmf.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTUSC3_MOUSE
AccessioniPrimary (citable) accession number: Q8BTV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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