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Q8BTF7

- DNLI4_MOUSE

UniProt

Q8BTF7 - DNLI4_MOUSE

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Protein
DNA ligase 4
Gene
Lig4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends By similarity.

Catalytic activityi

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactori

Magnesium By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei271 – 2711ATP By similarity
Active sitei273 – 2731N6-AMP-lysine intermediate By similarity
Binding sitei278 – 2781ATP By similarity
Binding sitei293 – 2931ATP By similarity
Metal bindingi331 – 3311Magnesium 1 Reviewed prediction
Metal bindingi427 – 4271Magnesium 2 Reviewed prediction
Binding sitei432 – 4321ATP By similarity
Binding sitei443 – 4431ATP By similarity
Binding sitei449 – 4491ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: MGI
  2. DNA binding Source: Ensembl
  3. DNA ligase (ATP) activity Source: MGI
  4. metal ion binding Source: UniProtKB-KW
  5. protein binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. DNA ligation Source: MGI
  2. DNA ligation involved in DNA recombination Source: MGI
  3. DNA ligation involved in DNA repair Source: MGI
  4. DNA repair Source: MGI
  5. T cell differentiation in thymus Source: MGI
  6. T cell receptor V(D)J recombination Source: MGI
  7. V(D)J recombination Source: MGI
  8. cell cycle Source: UniProtKB-KW
  9. cell division Source: UniProtKB-KW
  10. cell proliferation Source: MGI
  11. cellular response to lithium ion Source: Ensembl
  12. central nervous system development Source: MGI
  13. chromosome organization Source: MGI
  14. double-strand break repair Source: MGI
  15. double-strand break repair via nonhomologous end joining Source: MGI
  16. immunoglobulin V(D)J recombination Source: MGI
  17. in utero embryonic development Source: MGI
  18. isotype switching Source: MGI
  19. lagging strand elongation Source: RefGenome
  20. negative regulation of neuron apoptotic process Source: MGI
  21. neuron apoptotic process Source: MGI
  22. nucleotide-excision repair Source: RefGenome
  23. nucleotide-excision repair, DNA gap filling Source: Ensembl
  24. positive regulation of fibroblast proliferation Source: MGI
  25. positive regulation of neurogenesis Source: MGI
  26. pro-B cell differentiation Source: MGI
  27. response to X-ray Source: Ensembl
  28. response to gamma radiation Source: MGI
  29. response to ionizing radiation Source: MGI
  30. single strand break repair Source: Ensembl
  31. somatic stem cell maintenance Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligase 4 (EC:6.5.1.1)
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene namesi
Name:Lig4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1335098. Lig4.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. DNA ligase IV complex Source: RefGenome
  2. DNA-dependent protein kinase-DNA ligase 4 complex Source: MGI
  3. chromosome Source: RefGenome
  4. condensed chromosome Source: Ensembl
  5. cytoplasm Source: Ensembl
  6. focal adhesion Source: Ensembl
  7. nonhomologous end joining complex Source: UniProtKB
  8. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 911911DNA ligase 4
PRO_0000059577Add
BLAST

Proteomic databases

PaxDbiQ8BTF7.
PRIDEiQ8BTF7.

PTM databases

PhosphoSiteiQ8BTF7.

Expressioni

Gene expression databases

CleanExiMM_LIG4.
GenevestigatoriQ8BTF7.

Interactioni

Subunit structurei

Binds to XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 heteromer associates in a DNA-dependent manner with the DNA-dependent protein kinase complex DNA-PK, formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. Interacts with APLF By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ8BTF7.
SMRiQ8BTF7. Positions 6-605, 654-911.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini654 – 74390BRCT 1
Add
BLAST
Domaini808 – 911104BRCT 2
Add
BLAST

Sequence similaritiesi

Contains 2 BRCT domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1793.
GeneTreeiENSGT00750000117695.
HOGENOMiHOG000007831.
HOVERGENiHBG005516.
InParanoidiQ8BTF7.
KOiK10777.
OMAiHMCPSTK.
OrthoDBiEOG7BKCT2.
TreeFamiTF312980.

Family and domain databases

Gene3Di1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProiIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR021536. DNA_ligase_IV_dom.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
PF11411. DNA_ligase_IV. 1 hit.
[Graphical view]
SMARTiSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMiSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsiTIGR00574. dnl1. 1 hit.
PROSITEiPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BTF7-1 [UniParc]FASTAAdd to Basket

« Hide

MASSQTSQTV AAHVPFADLC STLERIQKGK DRAEKIRHFK EFLDSWRKFH    50
DALHKNRKDV TDSFYPAMRL ILPQLERERM AYGIKETMLA KLYIELLNLP 100
REGKDAQKLL NYRTPSGART DAGDFAMIAY FVLKPRCLQK GSLTIQQVNE 150
LLDLVASNNS GKKKDLVKKS LLQLITQSSA LEQKWLIRMI IKDLKLGISQ 200
QTIFSIFHND AVELHNVTTD LEKVCRQLHD PSVGLSDISI TLFSAFKPML 250
AAVADVERVE KDMKQQSFYI ETKLDGERMQ MHKDGALYRY FSRNGYNYTD 300
QFGESPQEGS LTPFIHNAFG TDVQACILDG EMMAYNPTTQ TFMQKGVKFD 350
IKRMVEDSGL QTCYSVFDVL MVNKKKLGRE TLRKRYEILS STFTPIQGRI 400
EIVQKTQAHT KKEVVDALND AIDKREEGIM VKHPLSIYKP DKRGEGWLKI 450
KPEYVSGLMD ELDVLIVGGY WGKGSRGGMM SHFLCAVAET PPPGDRPSVF 500
HTLCRVGSGY TMKELYDLGL KLAKYWKPFH KKSPPSSILC GTEKPEVYIE 550
PQNSVIVQIK AAEIVPSDMY KTGSTLRFPR IEKIRDDKEW HECMTLGDLE 600
QLRGKASGKL ATKHLHVGDD DEPREKRRKP ISKTKKAIRI IEHLKAPNLS 650
NVNKVSNVFE DVEFCVMSGL DGYPKADLEN RIAEFGGYIV QNPGPDTYCV 700
IAGSENVRVK NIISSDKNDV VKPEWLLECF KTKTCVPWQP RFMIHMCPST 750
KQHFAREYDC YGDSYFVDTD LDQLKEVFLG IKPSEQQTPE EMAPVIADLE 800
CRYSWDHSPL SMFRHYTIYL DLYAVINDLS SRIEATRLGI TALELRFHGA 850
KVVSCLSEGV SHVIIGEDQR RVTDFKIFRR MLKKKFKILQ ESWVSDSVDK 900
GELQEENQYL L 911
Length:911
Mass (Da):104,120
Last modified:October 3, 2012 - v2
Checksum:i20CDD0F6465355A2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti268 – 2681F → L in BAC26747. 1 Publication
Sequence conflicti758 – 7581Y → C in BAE28333. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030029 mRNA. Translation: BAC26747.1.
AK148081 mRNA. Translation: BAE28333.1.
AC138397 Genomic DNA. No translation available.
CH466566 Genomic DNA. Translation: EDL22038.1.
CH466566 Genomic DNA. Translation: EDL22040.1.
CCDSiCCDS22092.1.
RefSeqiNP_795927.2. NM_176953.3.
UniGeneiMm.80584.

Genome annotation databases

EnsembliENSMUST00000095476; ENSMUSP00000093130; ENSMUSG00000049717.
ENSMUST00000170033; ENSMUSP00000130807; ENSMUSG00000049717.
GeneIDi319583.
KEGGimmu:319583.
UCSCiuc009kul.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK030029 mRNA. Translation: BAC26747.1 .
AK148081 mRNA. Translation: BAE28333.1 .
AC138397 Genomic DNA. No translation available.
CH466566 Genomic DNA. Translation: EDL22038.1 .
CH466566 Genomic DNA. Translation: EDL22040.1 .
CCDSi CCDS22092.1.
RefSeqi NP_795927.2. NM_176953.3.
UniGenei Mm.80584.

3D structure databases

ProteinModelPortali Q8BTF7.
SMRi Q8BTF7. Positions 6-605, 654-911.
ModBasei Search...

PTM databases

PhosphoSitei Q8BTF7.

Proteomic databases

PaxDbi Q8BTF7.
PRIDEi Q8BTF7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000095476 ; ENSMUSP00000093130 ; ENSMUSG00000049717 .
ENSMUST00000170033 ; ENSMUSP00000130807 ; ENSMUSG00000049717 .
GeneIDi 319583.
KEGGi mmu:319583.
UCSCi uc009kul.1. mouse.

Organism-specific databases

CTDi 3981.
MGIi MGI:1335098. Lig4.

Phylogenomic databases

eggNOGi COG1793.
GeneTreei ENSGT00750000117695.
HOGENOMi HOG000007831.
HOVERGENi HBG005516.
InParanoidi Q8BTF7.
KOi K10777.
OMAi HMCPSTK.
OrthoDBi EOG7BKCT2.
TreeFami TF312980.

Miscellaneous databases

NextBioi 395028.
PROi Q8BTF7.
SOURCEi Search...

Gene expression databases

CleanExi MM_LIG4.
Genevestigatori Q8BTF7.

Family and domain databases

Gene3Di 1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProi IPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR021536. DNA_ligase_IV_dom.
IPR012340. NA-bd_OB-fold.
[Graphical view ]
Pfami PF00533. BRCT. 2 hits.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
PF11411. DNA_ligase_IV. 1 hit.
[Graphical view ]
SMARTi SM00292. BRCT. 2 hits.
[Graphical view ]
SUPFAMi SSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsi TIGR00574. dnl1. 1 hit.
PROSITEi PS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS00333. DNA_LIGASE_A2. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDNLI4_MOUSE
AccessioniPrimary (citable) accession number: Q8BTF7
Secondary accession number(s): G3UWC4, Q3UG76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: October 3, 2012
Last modified: September 3, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi