Q8BT07 (CEP55_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified July 9, 2014. Version 84. History...
Names and origin
|Protein names||Recommended name:|
Centrosomal protein of 55 kDa
|Organism||Mus musculus (Mouse) [Reference proteome]|
|Taxonomic identifier||10090 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus|
|Sequence length||462 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis By similarity.
Homodimer. Interacts (phosphorylated on Ser-423 and Ser-426) with PLK1. Interacts with AKAP9; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis. Interacts with MVB12A, VPS37B, VPS37C and VPS28 By similarity.
Cytoplasm › cytoskeleton › microtubule organizing center › centrosome › centriole By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome By similarity. Cleavage furrow By similarity. Midbody By similarity. Note: Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. Dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis By similarity.
There is a hierachy of phosphorylation, where both Ser-423 and Ser-426 are phosphorylated at the onset of mitosis, prior to Ser-434. Phosphorylation at Ser-423 and Ser-426 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-423, Ser-426 and Ser-434, is required for protein function at the final stages of cell division to complete cytokinesis successfully By similarity.
|Biological process||Cell cycle|
|Coding sequence diversity||Alternative splicing|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
Inferred from electronic annotation. Source: Ensemblmitotic nuclear division
Inferred from electronic annotation. Source: UniProtKB-KW
Inferred from electronic annotation. Source: UniProtKB-SubCellcleavage furrow
Inferred from electronic annotation. Source: UniProtKB-SubCellintercellular bridgemidbody
|Complete GO annotation...|
|This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]|
|Isoform 1 (identifier: Q8BT07-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
|Isoform 2 (identifier: Q8BT07-2) |
The sequence of this isoform differs from the canonical sequence as follows:
|Note: No experimental confirmation available.|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 462||462||Centrosomal protein of 55 kDa||PRO_0000238665|
|Region||157 – 235||79||Interaction with TSG101 By similarity|
|Region||160 – 214||55||Interaction with PDCD6IP By similarity|
|Region||354 – 462||109||Required for localization to the interphase centrosome and to the midbody during cytokinesis By similarity|
|Coiled coil||50 – 400||351||Potential|
Amino acid modifications
|Modified residue||423||1||Phosphoserine By similarity|
|Modified residue||426||1||Phosphoserine Ref.3|
|Modified residue||434||1||Phosphoserine; alternate Ref.3|
|Modified residue||434||1||Phosphoserine; by PLK1; alternate Probable|
|Alternative sequence||397||1||Missing in isoform 2.||VSP_018630|
|Natural variant||55||1||G → D. Ref.1 Ref.2|
|Sequence conflict||70||1||R → G in BAC40417. Ref.1|
|Sequence conflict||106||1||E → K in BAC40417. Ref.1|
|Sequence conflict||157||1||A → P in BAC25819. Ref.1|
|Sequence conflict||253||1||A → T in AAH26966. Ref.2|
|Sequence conflict||259||1||L → V in AAH26966. Ref.2|
|Sequence conflict||300||1||T → M in AAH26966. Ref.2|
|Sequence conflict||406||1||E → D in BAC25819. Ref.1|
|||"The transcriptional landscape of the mammalian genome."|
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-55.
Strain: C57BL/6J and NOD.
Tissue: Embryo, Lung and Thymus.
|||"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."|
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 76-462 (ISOFORM 1), VARIANT ASP-55.
Strain: Czech II and FVB/N.
Tissue: Mammary tumor.
|||"The phagosomal proteome in interferon-gamma-activated macrophages."|
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-426 AND SER-434, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
|+||Additional computationally mapped references.|
|Accession||Primary (citable) accession number: Q8BT07|
Secondary accession number(s): Q8C2J0 Q9DBZ8
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot