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UniProtKB - Q8BT07 (CEP55_MOUSE)

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Protein

Centrosomal protein of 55 kDa

Gene

Cep55

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mitotic exit and cytokinesis. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 55 kDa
Short name:
Cep55
Gene namesi
Name:Cep55
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1921357. Cep55.

Subcellular locationi

  • Cytoplasm › cytoskeleton › microtubule organizing center › centrosome › centriole By similarity
  • Cytoplasm › cytoskeleton › microtubule organizing center › centrosome By similarity
  • Cleavage furrow By similarity
  • Midbody › Midbody ring By similarity

    • Note: Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loses of affinity for the centrosome at the onset of prophase and diffuses throughout the cell. Dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis (By similarity).By similarity

GO - Cellular componenti

  • centriole Source: UniProtKB-SubCell
  • centrosome Source: MGI
  • cleavage furrow Source: UniProtKB-SubCell
  • Flemming body Source: UniProtKB
  • intercellular bridge Source: MGI
  • membrane Source: MGI
  • microtubule organizing center Source: MGI
  • midbody Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002386651 – 462Centrosomal protein of 55 kDaAdd BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei96PhosphoserineCombined sources1
Modified residuei99PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Modified residuei426PhosphoserineCombined sources1
Modified residuei428PhosphoserineCombined sources1
Modified residuei434Phosphoserine; by PLK1Combined sources1

Post-translational modificationi

There is a hierachy of phosphorylation, where both Ser-423 and Ser-426 are phosphorylated at the onset of mitosis, prior to Ser-434. Phosphorylation at Ser-423 and Ser-426 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-423, Ser-426 and Ser-434, is required for protein function at the final stages of cell division to complete cytokinesis successfully (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BT07.
PaxDbiQ8BT07.
PeptideAtlasiQ8BT07.
PRIDEiQ8BT07.

PTM databases

iPTMnetiQ8BT07.
PhosphoSitePlusiQ8BT07.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024989.
CleanExiMM_CEP55.
GenevisibleiQ8BT07. MM.

Interactioni

Subunit structurei

Homodimer. Interacts (phosphorylated on Ser-423 and Ser-426) with PLK1. Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry. Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis. Interacts with MVB12A, VPS37B, VPS37C and VPS28 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tex14Q7M6U311EBI-2552328,EBI-6674575

Protein-protein interaction databases

BioGridi216498. 42 interactors.
IntActiQ8BT07. 49 interactors.
STRINGi10090.ENSMUSP00000093802.

Structurei

3D structure databases

ProteinModelPortaliQ8BT07.
SMRiQ8BT07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 235Interaction with TSG101By similarityAdd BLAST79
Regioni160 – 214Interaction with PDCD6IPBy similarityAdd BLAST55
Regioni354 – 462Required for localization to the interphase centrosome and to the midbody during cytokinesisBy similarityAdd BLAST109

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili50 – 400Sequence analysisAdd BLAST351

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFBZ. Eukaryota.
ENOG41120Y2. LUCA.
GeneTreeiENSGT00510000047961.
HOGENOMiHOG000111547.
HOVERGENiHBG081092.
InParanoidiQ8BT07.
KOiK16456.
OMAiYDQQREV.
OrthoDBiEOG091G065Y.
PhylomeDBiQ8BT07.
TreeFamiTF331107.

Family and domain databases

InterProiView protein in InterPro
IPR022008. EABR.
PfamiView protein in Pfam
PF12180. EABR. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BT07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRSPKDLI KSKWGSRPSS SKSDTALEKF KGEIAAFKTS LDEITSGKGK 
        60         70         80         90        100
MAEKGRSRLL EKIQVLEAER EKNVYYLLEK DKEIQRLKDH LRSRYSSSSL 
       110        120        130        140        150
FEQLEEKTKE CEKKQQLLES LSKETDVLKN QLSATTKRLS ELESKASTLH 
       160        170        180        190        200
LSQSMPANCF NSSMNSIHEK EMQLKDALEK NQQWLVYDQQ REAYVKGLLA 
       210        220        230        240        250
KIFELEKRTE TAAASLTQQM KKIESEGYLQ VEKQKYDHLL ENAKKDLEVE 
       260        270        280        290        300
RQAVTQLRLE LDEFRRKYEE ARKEVEDLNQ LLSSQRKADI QHLEEDKQKT 
       310        320        330        340        350
ERIQKLREES SIFKGKLEEE RKRSEELLSQ VRILYDSLLK HQEEQARVAL 
       360        370        380        390        400
LEQQMQACTL DFENEKLDRQ NMQHQLYVIL KELRKAKSQI TQLESLKQLH 
       410        420        430        440        450
GFTITEQPFP LQREPESRVK ATSPKSPSAA LNDSLVECPK CSVQYPATEH 
       460 
RDLLVHVEYC MK
Length:462
Mass (Da):53,930
Last modified:May 30, 2006 - v2
Checksum:i5D2308BFCEADA08B
GO
Isoform 2 (identifier: Q8BT07-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-397: Missing.

Note: No experimental confirmation available.
Show »
Length:461
Mass (Da):53,802
Checksum:iC0816F19989B4FA1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70R → G in BAC40417 (PubMed:16141072).Curated1
Sequence conflicti106E → K in BAC40417 (PubMed:16141072).Curated1
Sequence conflicti157A → P in BAC25819 (PubMed:16141072).Curated1
Sequence conflicti253A → T in AAH26966 (PubMed:15489334).Curated1
Sequence conflicti259L → V in AAH26966 (PubMed:15489334).Curated1
Sequence conflicti300T → M in AAH26966 (PubMed:15489334).Curated1
Sequence conflicti406E → D in BAC25819 (PubMed:16141072).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti55G → D2 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018630397Missing in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004655 mRNA. Translation: BAB23446.1.
AK028216 mRNA. Translation: BAC25819.1.
AK088548 mRNA. Translation: BAC40417.1.
BC026966 mRNA. Translation: AAH26966.1.
BC031396 mRNA. Translation: AAH31396.1.
CCDSiCCDS29782.1. [Q8BT07-2]
CCDS50429.1. [Q8BT07-1]
RefSeqiNP_001157834.1. NM_001164362.1. [Q8BT07-1]
NP_082569.1. NM_028293.1. [Q8BT07-1]
NP_083036.2. NM_028760.2. [Q8BT07-2]
XP_006527459.1. XM_006527396.2. [Q8BT07-1]
XP_006527460.1. XM_006527397.3. [Q8BT07-1]
XP_006527461.1. XM_006527398.3. [Q8BT07-1]
XP_006527462.1. XM_006527399.2. [Q8BT07-1]
XP_006527463.1. XM_006527400.2. [Q8BT07-1]
XP_006527464.1. XM_006527401.2. [Q8BT07-1]
XP_011245683.1. XM_011247381.2. [Q8BT07-1]
UniGeneiMm.9916.

Genome annotation databases

EnsembliENSMUST00000096096; ENSMUSP00000093802; ENSMUSG00000024989. [Q8BT07-1]
ENSMUST00000116506; ENSMUSP00000112205; ENSMUSG00000024989. [Q8BT07-2]
ENSMUST00000169673; ENSMUSP00000127961; ENSMUSG00000024989. [Q8BT07-1]
GeneIDi74107.
KEGGimmu:74107.
UCSCiuc008hiy.2. mouse. [Q8BT07-1]
uc008hja.2. mouse. [Q8BT07-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCEP55_MOUSE
AccessioniPrimary (citable) accession number: Q8BT07
Secondary accession number(s): Q8C2J0
, Q8K2I8, Q8R2Y4, Q9DBZ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: July 5, 2017
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot