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Q8BT07

- CEP55_MOUSE

UniProt

Q8BT07 - CEP55_MOUSE

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Protein

Centrosomal protein of 55 kDa

Gene

Cep55

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis (By similarity).By similarity

GO - Biological processi

  1. establishment of protein localization Source: Ensembl
  2. mitotic cytokinesis Source: Ensembl
  3. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Centrosomal protein of 55 kDa
Short name:
Cep55
Gene namesi
Name:Cep55
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:1921357. Cep55.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cleavage furrow By similarity. Midbody By similarity
Note: Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loss of affinity for the centrosome at the onset of prophase and diffusion throughout the cell. Dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis (By similarity).By similarity

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. cytoplasm Source: UniProtKB-KW
  3. intercellular bridge Source: MGI
  4. midbody Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Centrosomal protein of 55 kDaPRO_0000238665Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei423 – 4231PhosphoserineBy similarity
Modified residuei426 – 4261Phosphoserine1 Publication
Modified residuei434 – 4341Phosphoserine; alternate1 Publication
Modified residuei434 – 4341Phosphoserine; by PLK1; alternate1 Publication

Post-translational modificationi

There is a hierachy of phosphorylation, where both Ser-423 and Ser-426 are phosphorylated at the onset of mitosis, prior to Ser-434. Phosphorylation at Ser-423 and Ser-426 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-423, Ser-426 and Ser-434, is required for protein function at the final stages of cell division to complete cytokinesis successfully (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BT07.
PaxDbiQ8BT07.
PRIDEiQ8BT07.

PTM databases

PhosphoSiteiQ8BT07.

Expressioni

Gene expression databases

BgeeiQ8BT07.
CleanExiMM_CEP55.
GenevestigatoriQ8BT07.

Interactioni

Subunit structurei

Homodimer. Interacts (phosphorylated on Ser-423 and Ser-426) with PLK1. Interacts with AKAP9; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PDCD6IP; the interaction is direct; CEP55 binds PDCD6IP in a 2:1 stoechiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis. Interacts with MVB12A, VPS37B, VPS37C and VPS28 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Tex14Q7M6U311EBI-2552328,EBI-6674575

Protein-protein interaction databases

BioGridi216498. 16 interactions.
IntActiQ8BT07. 22 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8BT07.
SMRiQ8BT07. Positions 167-208.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni157 – 23579Interaction with TSG101By similarityAdd
BLAST
Regioni160 – 21455Interaction with PDCD6IPBy similarityAdd
BLAST
Regioni354 – 462109Required for localization to the interphase centrosome and to the midbody during cytokinesisBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili50 – 400351Sequence AnalysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG135886.
GeneTreeiENSGT00510000047961.
HOGENOMiHOG000111547.
HOVERGENiHBG081092.
InParanoidiQ8BT07.
KOiK16456.
OMAiINISEVW.
OrthoDBiEOG7H4DTJ.
PhylomeDBiQ8BT07.
TreeFamiTF331107.

Family and domain databases

InterProiIPR022008. EABR.
[Graphical view]
PfamiPF12180. EABR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BT07-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSRSPKDLI KSKWGSRPSS SKSDTALEKF KGEIAAFKTS LDEITSGKGK
60 70 80 90 100
MAEKGRSRLL EKIQVLEAER EKNVYYLLEK DKEIQRLKDH LRSRYSSSSL
110 120 130 140 150
FEQLEEKTKE CEKKQQLLES LSKETDVLKN QLSATTKRLS ELESKASTLH
160 170 180 190 200
LSQSMPANCF NSSMNSIHEK EMQLKDALEK NQQWLVYDQQ REAYVKGLLA
210 220 230 240 250
KIFELEKRTE TAAASLTQQM KKIESEGYLQ VEKQKYDHLL ENAKKDLEVE
260 270 280 290 300
RQAVTQLRLE LDEFRRKYEE ARKEVEDLNQ LLSSQRKADI QHLEEDKQKT
310 320 330 340 350
ERIQKLREES SIFKGKLEEE RKRSEELLSQ VRILYDSLLK HQEEQARVAL
360 370 380 390 400
LEQQMQACTL DFENEKLDRQ NMQHQLYVIL KELRKAKSQI TQLESLKQLH
410 420 430 440 450
GFTITEQPFP LQREPESRVK ATSPKSPSAA LNDSLVECPK CSVQYPATEH
460
RDLLVHVEYC MK
Length:462
Mass (Da):53,930
Last modified:May 30, 2006 - v2
Checksum:i5D2308BFCEADA08B
GO
Isoform 2 (identifier: Q8BT07-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     397-397: Missing.

Note: No experimental confirmation available.

Show »
Length:461
Mass (Da):53,802
Checksum:iC0816F19989B4FA1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701R → G in BAC40417. (PubMed:16141072)Curated
Sequence conflicti106 – 1061E → K in BAC40417. (PubMed:16141072)Curated
Sequence conflicti157 – 1571A → P in BAC25819. (PubMed:16141072)Curated
Sequence conflicti253 – 2531A → T in AAH26966. (PubMed:15489334)Curated
Sequence conflicti259 – 2591L → V in AAH26966. (PubMed:15489334)Curated
Sequence conflicti300 – 3001T → M in AAH26966. (PubMed:15489334)Curated
Sequence conflicti406 – 4061E → D in BAC25819. (PubMed:16141072)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti55 – 551G → D.2 Publications

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei397 – 3971Missing in isoform 2. 1 PublicationVSP_018630

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004655 mRNA. Translation: BAB23446.1.
AK028216 mRNA. Translation: BAC25819.1.
AK088548 mRNA. Translation: BAC40417.1.
BC026966 mRNA. Translation: AAH26966.1.
BC031396 mRNA. Translation: AAH31396.1.
CCDSiCCDS29782.1. [Q8BT07-2]
CCDS50429.1. [Q8BT07-1]
RefSeqiNP_001157834.1. NM_001164362.1. [Q8BT07-1]
NP_082569.1. NM_028293.1. [Q8BT07-1]
NP_083036.2. NM_028760.2. [Q8BT07-2]
XP_006527459.1. XM_006527396.1. [Q8BT07-1]
XP_006527460.1. XM_006527397.1. [Q8BT07-1]
XP_006527461.1. XM_006527398.1. [Q8BT07-1]
XP_006527462.1. XM_006527399.1. [Q8BT07-1]
XP_006527463.1. XM_006527400.1. [Q8BT07-1]
XP_006527464.1. XM_006527401.1. [Q8BT07-1]
UniGeneiMm.9916.

Genome annotation databases

EnsembliENSMUST00000096096; ENSMUSP00000093802; ENSMUSG00000024989. [Q8BT07-1]
ENSMUST00000116506; ENSMUSP00000112205; ENSMUSG00000024989. [Q8BT07-2]
ENSMUST00000169673; ENSMUSP00000127961; ENSMUSG00000024989. [Q8BT07-1]
GeneIDi74107.
KEGGimmu:74107.
UCSCiuc008hiy.2. mouse. [Q8BT07-1]
uc008hja.2. mouse. [Q8BT07-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004655 mRNA. Translation: BAB23446.1 .
AK028216 mRNA. Translation: BAC25819.1 .
AK088548 mRNA. Translation: BAC40417.1 .
BC026966 mRNA. Translation: AAH26966.1 .
BC031396 mRNA. Translation: AAH31396.1 .
CCDSi CCDS29782.1. [Q8BT07-2 ]
CCDS50429.1. [Q8BT07-1 ]
RefSeqi NP_001157834.1. NM_001164362.1. [Q8BT07-1 ]
NP_082569.1. NM_028293.1. [Q8BT07-1 ]
NP_083036.2. NM_028760.2. [Q8BT07-2 ]
XP_006527459.1. XM_006527396.1. [Q8BT07-1 ]
XP_006527460.1. XM_006527397.1. [Q8BT07-1 ]
XP_006527461.1. XM_006527398.1. [Q8BT07-1 ]
XP_006527462.1. XM_006527399.1. [Q8BT07-1 ]
XP_006527463.1. XM_006527400.1. [Q8BT07-1 ]
XP_006527464.1. XM_006527401.1. [Q8BT07-1 ]
UniGenei Mm.9916.

3D structure databases

ProteinModelPortali Q8BT07.
SMRi Q8BT07. Positions 167-208.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 216498. 16 interactions.
IntActi Q8BT07. 22 interactions.

PTM databases

PhosphoSitei Q8BT07.

Proteomic databases

MaxQBi Q8BT07.
PaxDbi Q8BT07.
PRIDEi Q8BT07.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000096096 ; ENSMUSP00000093802 ; ENSMUSG00000024989 . [Q8BT07-1 ]
ENSMUST00000116506 ; ENSMUSP00000112205 ; ENSMUSG00000024989 . [Q8BT07-2 ]
ENSMUST00000169673 ; ENSMUSP00000127961 ; ENSMUSG00000024989 . [Q8BT07-1 ]
GeneIDi 74107.
KEGGi mmu:74107.
UCSCi uc008hiy.2. mouse. [Q8BT07-1 ]
uc008hja.2. mouse. [Q8BT07-2 ]

Organism-specific databases

CTDi 55165.
MGIi MGI:1921357. Cep55.

Phylogenomic databases

eggNOGi NOG135886.
GeneTreei ENSGT00510000047961.
HOGENOMi HOG000111547.
HOVERGENi HBG081092.
InParanoidi Q8BT07.
KOi K16456.
OMAi INISEVW.
OrthoDBi EOG7H4DTJ.
PhylomeDBi Q8BT07.
TreeFami TF331107.

Miscellaneous databases

NextBioi 339796.
PROi Q8BT07.
SOURCEi Search...

Gene expression databases

Bgeei Q8BT07.
CleanExi MM_CEP55.
Genevestigatori Q8BT07.

Family and domain databases

InterProi IPR022008. EABR.
[Graphical view ]
Pfami PF12180. EABR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT ASP-55.
    Strain: C57BL/6J and NOD.
    Tissue: Embryo, Lung and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 76-462 (ISOFORM 1), VARIANT ASP-55.
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-426 AND SER-434, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCEP55_MOUSE
AccessioniPrimary (citable) accession number: Q8BT07
Secondary accession number(s): Q8C2J0
, Q8K2I8, Q8R2Y4, Q9DBZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: October 29, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3