Q8BST6 (PELI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
E3 ubiquitin-protein ligase pellino homolog 2
Short name=Pellino-2
EC=6.3.2.-

Gene names

Name:

Peli2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates IL1B-induced IRAK1 'Lys-63'-linked polyubiquitination and possibly 'Lys-48'-linked ubiquitination. May be important for LPS- and IL1B-induced MAP3K7-dependent, but not MAP3K3-dependent, NF-kappa-B activation. Can activate the MAP (mitogen activated protein) kinase pathway leading to activation of ELK1. Ref.4 Ref.6
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with TRAF6, IRAK4 and MAP3K7 By similarity. Interacts with IRAK1. Interacts with BCL10; this interaction is impaired by SOCS3. Ref.4 Ref.5
Tissue specificity	Widely expressed both in embryos and adult. Weakly or not expressed in spleen and thymus. Ref.4
Domain	The atypical FHA domain contains a 'wing' insert and mediates binding to threonine-phosphorylated IRAK1 By similarity.
Post-translational modification	Phosphorylated by IRAK1 and IRAK4 enhancing its E3 ligase activity By similarity.
Sequence similarities	Belongs to the pellino family. Contains 1 FHA domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Alternative splicing
Molecular function	Ligase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Toll signaling pathway Inferred from electronic annotation. Source: InterPro protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from direct assay PubMed 16831874. Source: UniProtKB membrane Inferred from direct assay PubMed 16831874. Source: UniProtKB
Molecular_function	ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Irak1	Q62406	2	EBI-448554,EBI-448533

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BST6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BST6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8BST6-3)

The sequence of this isoform differs from the canonical sequence as follows:
     70-155: AISSRGHHSI...RNEPYTARIF → LPAAKHYYNE...KKKIFFSCWS
     156-419: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	E3 ubiquitin-protein ligase pellino homolog 2		PRO_0000194175
Regions
	Domain	15 – 202	188	FHA; atypical
Natural variations
	Alternative sequence	1 – 100	100	Missing in isoform 2.		VSP_008636
	Alternative sequence	70 – 155	86	AISSR…TARIF → LPAAKHYYNEADSESLSALT LKVRDFLTGECSQRREYRDP AFSREGASGSAQLVAQAFLI CPLSYTIVKQEQIRCLKKKI FFSCWS in isoform 3.		VSP_008637
	Alternative sequence	156 – 419	264	Missing in isoform 3.		VSP_008638
Experimental info
	Sequence conflict	7	1	E → K in BAC27024. Ref.2
	Sequence conflict	11	1	A → T in BAC27024. Ref.2
	Sequence conflict	20	1	R → G in BAC38472. Ref.2
	Sequence conflict	38 – 39	2	RR → KK in AAG15392. Ref.1
	Sequence conflict	44	1	A → T in AAG15392. Ref.1
	Sequence conflict	320	1	S → H in AAG15392. Ref.1
	Sequence conflict	327	1	T → A in AAH27062. Ref.3
	Sequence conflict	338	1	R → M in AAG15392. Ref.1
	Sequence conflict	364	1	A → V in AAG15392. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 24, 2003. Version 2. Checksum: 786C92C28C38D0CB	FASTA	419	46,272
	10 20 30 40 50 60 MFSPGQEEPS APNKEPVKYR ELVVLGYNGA LPNGDRGRRK SRFALYKRTY ASGVKPSTIH 70 80 90 100 110 120 MVSTPQASKA ISSRGHHSIS YTLSRSQTVV VEYTHDKDTD MFQVGRSTES PIDFVVTDTV 130 140 150 160 170 180 SGGQNEDAQI TQSTISRFAC RIVCDRNEPY TARIFAAGFD SSKNIFLGEK AAKWKNPDGH 190 200 210 220 230 240 MDGLTTNGVL VMHPQGGFTE ESQPGVWREI SVCGDVYTLR ETRSAQQRGK LVESETNVLQ 250 260 270 280 290 300 DGSLIDLCGA TLLWRTADGL FHAPTQKHIE ALRQEINAAR PQCPVGLNTL AFPSINRKEV 310 320 330 340 350 360 VEEKQPWAYL SCGHVHGYHS WGHRSDTEAN ERECPMCRTV GPYVPLWLGC EAGFYVDAGP 370 380 390 400 410 PTHAFTPCGH VCSEKSAKYW SQIPLPHGTH AFHAACPFCA TQLVGEQNCI KLIFQGPVD
	Isoform 2 [UniParc]. Checksum: 7FFA0991F1FA0950	FASTA	319	35,165
	10 20 30 40 50 60 MFQVGRSTES PIDFVVTDTV SGGQNEDAQI TQSTISRFAC RIVCDRNEPY TARIFAAGFD 70 80 90 100 110 120 SSKNIFLGEK AAKWKNPDGH MDGLTTNGVL VMHPQGGFTE ESQPGVWREI SVCGDVYTLR 130 140 150 160 170 180 ETRSAQQRGK LVESETNVLQ DGSLIDLCGA TLLWRTADGL FHAPTQKHIE ALRQEINAAR 190 200 210 220 230 240 PQCPVGLNTL AFPSINRKEV VEEKQPWAYL SCGHVHGYHS WGHRSDTEAN ERECPMCRTV 250 260 270 280 290 300 GPYVPLWLGC EAGFYVDAGP PTHAFTPCGH VCSEKSAKYW SQIPLPHGTH AFHAACPFCA 310 TQLVGEQNCI KLIFQGPVD
	Isoform 3 [UniParc]. Checksum: E443ACF3246FA349	FASTA	155	17,403
	10 20 30 40 50 60 MFSPGQEEPS APNKEPVKYR ELVVLGYNGA LPNGDRGRRK SRFALYKRTY ASGVKPSTIH 70 80 90 100 110 120 MVSTPQASKL PAAKHYYNEA DSESLSALTL KVRDFLTGEC SQRREYRDPA FSREGASGSA 130 140 150 QLVAQAFLIC PLSYTIVKQE QIRCLKKKIF FSCWS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Assignment of homologous genes, Peli1/PELI1 and Peli2/PELI2, for the Pelle adaptor protein Pellino to mouse chromosomes 11 and 14 and human chromosomes 2p13.3 and 14q21, respectively, by physical and radiation hybrid mapping." Resch K., Jockusch H., Schmitt-John T. Cytogenet. Cell Genet. 92:172-174(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: C57BL/6J. Tissue: Cerebellum, Epididymis and Pituitary.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Mammary tumor.
[4]	"Mouse pellino-2 modulates IL-1 and lipopolysaccharide signaling." Yu K.-Y., Kwon H.-J., Norman D.A.M., Vig E., Goebl M.G., Harrington M.A. J. Immunol. 169:4075-4078(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH IRAK1.
[5]	"BCL10 mediates lipopolysaccharide/toll-like receptor-4 signaling through interaction with Pellino2." Liu Y., Dong W., Chen L., Xiang R., Xiao H., De G., Wang Z., Qi Y. J. Biol. Chem. 279:37436-37444(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BCL10.
[6]	"Pellino 2 is critical for Toll-like receptor/interleukin-1 receptor (TLR/IL-1R)-mediated post-transcriptional control." Kim T.W., Yu M., Zhou H., Cui W., Wang J., DiCorleto P., Fox P., Xiao H., Li X. J. Biol. Chem. 287:25686-25695(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF302504 mRNA. Translation: AAG15392.1. AK030564 mRNA. Translation: BAC27024.1. AK033815 mRNA. Translation: BAC28485.1. AK082342 mRNA. Translation: BAC38472.1. BC027062 mRNA. Translation: AAH27062.1.
IPI	IPI00380270. IPI00380271. IPI00380272.
RefSeq	NP_291080.2. NM_033602.2.
UniGene	Mm.296457.
3D structure databases
ProteinModelPortal	Q8BST6.
SMR	Q8BST6. Positions 15-257.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8BST6. 1 interaction.
PTM databases
PhosphoSite	Q8BST6.
Proteomic databases
PaxDb	Q8BST6.
PRIDE	Q8BST6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	93834.
KEGG	mmu:93834.
UCSC	uc007tjh.1. mouse. uc007tji.1. mouse.
Organism-specific databases
CTD	57161.
MGI	MGI:1891445. Peli2.
Phylogenomic databases
eggNOG	NOG258040.
HOGENOM	HOG000234110.
HOVERGEN	HBG053559.
InParanoid	Q8BST6.
KO	K11964.
OrthoDB	EOG466VKV.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
CleanEx	MM_PELI2.
Genevestigator	Q8BST6.
GermOnline	ENSMUSG00000021846. Mus musculus.
Family and domain databases
InterPro	IPR006800. Pellino_fam. [Graphical view]
PANTHER	PTHR12098. PTHR12098. 1 hit.
Pfam	PF04710. Pellino. 1 hit. [Graphical view]
PIRSF	PIRSF038886. Pellino. 1 hit.
PROSITE	PS50006. FHA_DOMAIN. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	351677.
SOURCE	Search...

Entry information

Entry name

PELI2_MOUSE

Accession

Primary (citable) accession number: Q8BST6
Secondary accession number(s): Q8C4F2 , Q8CC65, Q8R2X4, Q9ERJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	October 24, 2003
Last modified:	May 1, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program