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Q8BSQ9 (PB1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein polybromo-1
Alternative name(s):
BRG1-associated factor 180
Short name=BAF180
Gene names
Name:Pbrm1
Synonyms:Baf180, Pb1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1634 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology) By similarity. Acts as a negative regulator of cell proliferation By similarity.

Subunit structure

Component of the SWI/SNF-B (PBAF) chromatin-remodeling complex, which contains at least SMARCA4/BRG1, SMARCB1/SNF5/INI1/BAF47, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PB1/BAF180, ARID2/BAF200, ARID1A/BAF250A or ARID1B/BAF250B and actin. Interacts with acetylated 'Lys-14' of histone H3 (H3K14ac), and may also interact with other acetylated or methylated Lys residues on histone H3 By similarity. Interacts with PHF10/BAF45A. Ref.5

Subcellular location

Nucleus By similarity.

Developmental stage

Expressed ubiquitously throughout the developing spinal cord, brain and other embryonic tissues at E10.5-E16.5. Ref.5

Sequence similarities

Contains 2 BAH domains.

Contains 6 bromo domains.

Contains 1 HMG box DNA-binding domain.

Sequence caution

The sequence AAH29037.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

The sequence AAH55456.1 differs from that shown. Reason: Contaminating sequence.

The sequence AAH55708.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DiseaseTumor suppressor
   DomainBromodomain
Repeat
   LigandDNA-binding
   Molecular functionChromatin regulator
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processblood vessel remodeling

Traceable author statement PubMed 20299672. Source: DFLAT

cardiac vascular smooth muscle cell development

Traceable author statement PubMed 20299672. Source: DFLAT

chromatin modification

Inferred from electronic annotation. Source: UniProtKB-KW

epicardium-derived cardiac endothelial cell differentiation

Traceable author statement PubMed 20299672. Source: DFLAT

heart development

Inferred from mutant phenotype PubMed 15601824. Source: MGI

heart morphogenesis

Traceable author statement PubMed 20299672. Source: DFLAT

negative regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

placenta development

Inferred from mutant phenotype PubMed 15601824. Source: MGI

positive regulation of transcription, DNA-templated

Traceable author statement PubMed 20299672. Source: DFLAT

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

vasculogenesis involved in coronary vascular morphogenesis

Traceable author statement PubMed 20299672. Source: DFLAT

   Cellular_componentkinetochore

Inferred from direct assay PubMed 14519686. Source: MGI

nucleus

Inferred by curator PubMed 15601824. Source: MGI

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

chromatin binding

Inferred from direct assay PubMed 15601824. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BSQ9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BSQ9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     300-332: RTASNLAAARLTGPSHNKSSLGEERNPTSKYYR → S

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16341634Protein polybromo-1
PRO_0000351134

Regions

Domain64 – 13471Bromo 1
Domain200 – 27071Bromo 2
Domain400 – 47071Bromo 3
Domain538 – 60871Bromo 4
Domain676 – 74671Bromo 5
Domain792 – 86271Bromo 6
Domain956 – 1074119BAH 1
Domain1156 – 1272117BAH 2
DNA binding1379 – 144769HMG box
Compositional bias630 – 6334Poly-Asp
Compositional bias1468 – 1599132Pro-rich

Amino acid modifications

Modified residue101Phosphoserine By similarity
Modified residue391Phosphoserine By similarity
Modified residue1311Phosphoserine By similarity
Modified residue3191Phosphoserine By similarity
Modified residue3531Phosphoserine Ref.6
Modified residue3551Phosphoserine Ref.6
Modified residue3711Phosphoserine By similarity
Modified residue3751Phosphoserine By similarity
Modified residue4141N6-acetyllysine Ref.9
Modified residue4981Phosphoserine By similarity
Modified residue6361Phosphoserine By similarity
Modified residue6481Phosphoserine By similarity
Modified residue12891Phosphotyrosine Ref.4
Modified residue14051Phosphoserine By similarity

Natural variations

Alternative sequence300 – 33233RTASN…SKYYR → S in isoform 2.
VSP_035471

Experimental info

Sequence conflict4171Y → K in AAH23452. Ref.2
Sequence conflict6951D → E in BAC27136. Ref.3

Secondary structure

.................. 1634
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: A905963F22ECD75E

FASTA1,634187,188
        10         20         30         40         50         60 
MGSKRRRATS PSSSVSGDFD DGHHSVPTPG PSRKRRRLSN LPTVDPIAVC HELYNTIRDY 

        70         80         90        100        110        120 
KDEQGRLLCE LFIRAPKRRN QPDYYEVVSQ PIDLMKIQQK LKMEEYDDVN LLTADFQLLF 

       130        140        150        160        170        180 
NNAKAYYKPD SPEYKAACKL WDLYLRTRNE FVQKGEADDE DDDEDGQDNQ GTLADGSSPG 

       190        200        210        220        230        240 
YLKEILEQLL EAIVVATNPS GRLISELFQK LPSKVQYPDY YAIIKEPIDL KTIAQRIQNG 

       250        260        270        280        290        300 
SYKSIHAMAK DIDLLAKNAK TYNEPGSQVF KDANSIKKIF YMKKAEIEHH EMTKSSLRIR 

       310        320        330        340        350        360 
TASNLAAARL TGPSHNKSSL GEERNPTSKY YRNKRAVQGG RLSAITMALQ YGSESEEDAA 

       370        380        390        400        410        420 
LAAARYEEGE SEAESITSFM DVSNPFHQLY DTVRSCRNHQ GQLIAEPFFH LPSKKKYPDY 

       430        440        450        460        470        480 
YQQIKMPISL QQIRTKLKNQ EYETLDHLEC DLNLMFENAK RYNVPNSAIY KRVLKLQQVM 

       490        500        510        520        530        540 
QAKKKELARR DDIEDGDSMI SSATSDTGSA KRKSKKNIRK QRMKILFNVV LEAREPGSGR 

       550        560        570        580        590        600 
RLCDLFMVKP SKKDYPDYYK IILEPMDLKI IEHNIRNDKY AGEEGMMEDM KLMFRNARHY 

       610        620        630        640        650        660 
NEEGSQVYND AHILEKLLKD KRKELGPLPD DDDMASPKLK LSRKSGVSPK KSKYMTPMQQ 

       670        680        690        700        710        720 
KLNEVYEAVK NYTDKRGRRL SAIFLRLPSR SELPDYYLTI KKPMDMEKIR SHMMANKYQD 

       730        740        750        760        770        780 
IDSMVEDFVM MFNNACTYNE PESLIYKDAL VLHKVLLETR RDLEGDEDSH VPNVTLLIQE 

       790        800        810        820        830        840 
LIHNLFVSVM SHQDDEGRCY SDSLAEIPAV DPNSPNKPPL TFDIIRKNVE SNRYRRLDLF 

       850        860        870        880        890        900 
QEHMFEVLER ARRMNRTDSE IYEDAVELQQ FFIRIRDELC KNGEILLSPA LSYTTKHLHN 

       910        920        930        940        950        960 
DVEKEKKEKL PKEIEEDKLK REEEKREAEK SEDSSGTTGL SGLHRTYSQD CSFKNSMYHV 

       970        980        990       1000       1010       1020 
GDYVYVEPAE ANLQPHIVCI ERLWEDSAGE KWLYGCWFYR PNETFHLATR KFLEKEVFKS 

      1030       1040       1050       1060       1070       1080 
DYYNKVPVSK ILGKCVVMFV KEYFKLCPEN FRDEDVFVCE SRYSAKTKSF KKIKLWTMPI 

      1090       1100       1110       1120       1130       1140 
SSVRFVPRDV PLPVVRVASV FANADKGDDE KNTDNSDDNR AEDNFNLEKE KEDVPVEMSN 

      1150       1160       1170       1180       1190       1200 
GEPGCHYFEQ LRYNDMWLKV GDCVFIKSHG LVRPRVGRIE KVWVRDGAAY FYGPIFIHPE 

      1210       1220       1230       1240       1250       1260 
ETEHEPTKMF YKKEVFLSNL EETCPMSCIL GKCAVLSFKD FLSCRPTEIP ENDILLCESR 

      1270       1280       1290       1300       1310       1320 
YNESDKQMKK FKGLKRFSLS AKVVDDEIYY FRKPIIPQKE PSPLLEKKIQ LLEAKFAELE 

      1330       1340       1350       1360       1370       1380 
GGDDDIEEMG EEDSEVIEAP SLPQLQTPLA NELDLMPYTP PQSTPKSAKG SAKKESSKRK 

      1390       1400       1410       1420       1430       1440 
INMSGYILFS SEMRAVIKAQ HPDYSFGELS RLVGTEWRNL ETAKKAEYEG MMGGYPPGLP 

      1450       1460       1470       1480       1490       1500 
PLQGPVDGLV SMGSMQPLHP GGPPPHHLPP GVPGLPGIPP PGVMNQGVAP MVGTPAPGGS 

      1510       1520       1530       1540       1550       1560 
PYGQQVGVLG PPGQQAPPPY PGPHPAGPPV IQQPTTPMFV APPPKTQRLL HSEAYLKYIE 

      1570       1580       1590       1600       1610       1620 
GLSAESNSIS KWDQTLAARR RDVHLSKEQE SRLPSHWLKS KGAHTTMADA LWRLRDLMLR 

      1630 
DTLNIRQAYN LENV 

« Hide

Isoform 2 [UniParc].

Checksum: 29EEFCA9EF8A94C9
Show »

FASTA1,602183,659

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6, Czech II and FVB/N.
Tissue: Brain and Mammary tumor.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-919 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 205-512 (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Embryo and Tongue.
[4]"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1289, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Mast cell.
[5]"An essential switch in subunit composition of a chromatin remodeling complex during neural development."
Lessard J., Wu J.I., Ranish J.A., Wan M., Winslow M.M., Staahl B.T., Wu H., Aebersold R., Graef I.A., Crabtree G.R.
Neuron 55:201-215(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH PHF10, DEVELOPMENTAL STAGE.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-353 AND SER-355, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[9]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-414, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[10]"Solution structure of the fifth bromodomain from mouse polybromo-1."
RIKEN structural genomics initiative (RSGI)
Submitted (OCT-2007) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 646-762.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC154446 Genomic DNA. No translation available.
AC154727 Genomic DNA. No translation available.
BC023452 mRNA. Translation: AAH23452.1.
BC029037 mRNA. Translation: AAH29037.1. Different termination.
BC055456 mRNA. Translation: AAH55456.1. Different termination.
BC055708 mRNA. Translation: AAH55708.1. Different initiation.
BF451491 mRNA. No translation available.
CN530699 mRNA. No translation available.
AK009582 mRNA. Translation: BAB26374.2.
AK030781 mRNA. Translation: BAC27136.2.
AK030252 mRNA. No translation available.
AK166588 mRNA. No translation available.
UniGeneMm.27913.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YQDNMR-A650-762[»]
ProteinModelPortalQ8BSQ9.
SMRQ8BSQ9. Positions 43-154, 176-292, 388-494, 510-628, 651-766, 773-914, 957-1102.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48885N.
IntActQ8BSQ9. 2 interactions.
MINTMINT-4112204.

PTM databases

PhosphoSiteQ8BSQ9.

Proteomic databases

PaxDbQ8BSQ9.
PRIDEQ8BSQ9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000112094; ENSMUSP00000107723; ENSMUSG00000042323. [Q8BSQ9-2]
ENSMUST00000112095; ENSMUSP00000107724; ENSMUSG00000042323. [Q8BSQ9-1]
UCSCuc007swl.1. mouse. [Q8BSQ9-1]
uc007swm.1. mouse. [Q8BSQ9-2]

Organism-specific databases

MGIMGI:1923998. Pbrm1.

Phylogenomic databases

eggNOGCOG5076.
GeneTreeENSGT00390000003017.
HOVERGENHBG079860.
OrthoDBEOG78H3S6.

Gene expression databases

ArrayExpressQ8BSQ9.
BgeeQ8BSQ9.
GenevestigatorQ8BSQ9.

Family and domain databases

Gene3D1.10.30.10. 1 hit.
1.20.920.10. 6 hits.
InterProIPR001025. BAH_dom.
IPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR009071. HMG_box_dom.
[Graphical view]
PfamPF01426. BAH. 2 hits.
PF00439. Bromodomain. 6 hits.
PF00505. HMG_box. 1 hit.
[Graphical view]
PRINTSPR00503. BROMODOMAIN.
SMARTSM00439. BAH. 2 hits.
SM00297. BROMO. 6 hits.
SM00398. HMG. 1 hit.
[Graphical view]
SUPFAMSSF47095. SSF47095. 1 hit.
SSF47370. SSF47370. 6 hits.
PROSITEPS51038. BAH. 2 hits.
PS00633. BROMODOMAIN_1. 5 hits.
PS50014. BROMODOMAIN_2. 6 hits.
PS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPBRM1. mouse.
EvolutionaryTraceQ8BSQ9.
PROQ8BSQ9.
SOURCESearch...

Entry information

Entry namePB1_MOUSE
AccessionPrimary (citable) accession number: Q8BSQ9
Secondary accession number(s): E9QPW1 expand/collapse secondary AC list , Q05C64, Q05CL6, Q7TMP1, Q7TNU2, Q9CV51
Entry history
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 92 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot