Q8BSP2 (CNDH2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Condensin-2 complex subunit H2

Alternative name(s):
Kleisin-beta
Non-SMC condensin II complex subunit H2

Gene names

Name:	Ncaph2
Synonyms:	D15Ertd785e

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture By similarity. Seems to have lineage-specific role in T-cell development By similarity.
Subunit structure	Component of the condensin-2 complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits, NCAPG2, NCAPH2 and NCAPD3 that probably regulate the complex By similarity.
Subcellular location	Nucleus By similarity.
Involvement in disease	Defects in Ncaph2 are the cause of the nessy phenotype which is characterized by a specific defect in T-cell development. Nessy thymuses are smaller, with corticomedullary junctions less well defined, and cortical cells sparser than in wild-type. The thymocyte defect is typified by an increased proportion of CD4-CD8- DN T-cell progenitors. Only thymocyte differentiation is affected in Nessy mice and not cell differentiation.
Sequence similarities	Belongs to the CND2 H2 (condensin-2 subunit 2) family.
Sequence caution	The sequence AAH03900.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	DNA condensation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Disease	Disease mutation
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell differentiation in thymus Inferred from mutant phenotype Ref.3. Source: MGI chromosome condensation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BSP2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BSP2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     434-434: A → AA

Isoform 3 (identifier: Q8BSP2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-36: MEDVEVRFAHLLQPIRDLTKNWEVDVAAQLGEYLEE → MWRCALLTSCSPSGISLRTGRWTWRHSW

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 607	607	Condensin-2 complex subunit H2		PRO_0000326242
Amino acid modifications
	Modified residue	19	1	Phosphothreonine By similarity
	Modified residue	95	1	Phosphoserine By similarity
	Modified residue	494	1	Phosphoserine Ref.4
Natural variations
	Alternative sequence	1 – 36	36	MEDVE…EYLEE → MWRCALLTSCSPSGISLRTG RWTWRHSW in isoform 3.		VSP_032641
	Alternative sequence	434	1	A → AA in isoform 2.		VSP_032642
	Natural variant	15	1	I → N in Nessy. Ref.3
Experimental info
	Sequence conflict	83	1	D → N in BAC39479. Ref.1
	Sequence conflict	208	1	V → M in BAC29736. Ref.1
	Sequence conflict	218	1	K → E in BAC37919. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 616CA03BDD647852	FASTA	607	68,945
	10 20 30 40 50 60 MEDVEVRFAH LLQPIRDLTK NWEVDVAAQL GEYLEELDQI CISFDEGKTT MNFIEAALLI 70 80 90 100 110 120 QGSACVYSKK VEYLYSLVYQ ALDFISGKRR AKQLSLVQED GSKKTVNSET PCETENEFLS 130 140 150 160 170 180 LDDFPDSRAN VDLKNDQASS ELLIIPLLPM ALVAPDEVEK NSSPLYSCQG DILASRKDFR 190 200 210 220 230 240 MNTCMPNPRG CFMLDPVGMC PVEPVVPVEP YPMSRSQKDP EDAEEQPMEV SRNGSPVPVP 250 260 270 280 290 300 DISQEPDGPA LSGGEEDAED GAEPLEVALE PAEPRTSQQS AILPRRYMLR ERQGAPEPAS 310 320 330 340 350 360 RLQETPDPWQ SLDPFDSLES KVFQKGKPYS VPPGVEEAPG QKRKRKGATK LQDFHKWYLD 370 380 390 400 410 420 AYAEHPDGRR ARRKGPTFAD MEVLYWKHVK EQLETLQKLR RRKINERWLP GAKQDLWPTE 430 440 450 460 470 480 EDRLEESLED LGVADDFLEP EEYVEEPAGV MPEEAADLDA EAMPESLRYE ELVRRNVELF 490 500 510 520 530 540 IATSQKFIQE TELSQRIRDW EDTIQPLLQE QEQHVPFDIH IYGDQLASRF PQLNEWCPFS 550 560 570 580 590 600 ELVAGQPAFE VCRSMLASLQ LANDYTVEIT QQPGLEAAVD TMSLRLLTHQ RAHTRFQTYA APSMAQP
	Isoform 2 [UniParc]. Checksum: 58FD3EE7F8E59483	FASTA	608	69,017
	10 20 30 40 50 60 MEDVEVRFAH LLQPIRDLTK NWEVDVAAQL GEYLEELDQI CISFDEGKTT MNFIEAALLI 70 80 90 100 110 120 QGSACVYSKK VEYLYSLVYQ ALDFISGKRR AKQLSLVQED GSKKTVNSET PCETENEFLS 130 140 150 160 170 180 LDDFPDSRAN VDLKNDQASS ELLIIPLLPM ALVAPDEVEK NSSPLYSCQG DILASRKDFR 190 200 210 220 230 240 MNTCMPNPRG CFMLDPVGMC PVEPVVPVEP YPMSRSQKDP EDAEEQPMEV SRNGSPVPVP 250 260 270 280 290 300 DISQEPDGPA LSGGEEDAED GAEPLEVALE PAEPRTSQQS AILPRRYMLR ERQGAPEPAS 310 320 330 340 350 360 RLQETPDPWQ SLDPFDSLES KVFQKGKPYS VPPGVEEAPG QKRKRKGATK LQDFHKWYLD 370 380 390 400 410 420 AYAEHPDGRR ARRKGPTFAD MEVLYWKHVK EQLETLQKLR RRKINERWLP GAKQDLWPTE 430 440 450 460 470 480 EDRLEESLED LGVAADDFLE PEEYVEEPAG VMPEEAADLD AEAMPESLRY EELVRRNVEL 490 500 510 520 530 540 FIATSQKFIQ ETELSQRIRD WEDTIQPLLQ EQEQHVPFDI HIYGDQLASR FPQLNEWCPF 550 560 570 580 590 600 SELVAGQPAF EVCRSMLASL QLANDYTVEI TQQPGLEAAV DTMSLRLLTH QRAHTRFQTY AAPSMAQP
	Isoform 3 [UniParc]. Checksum: 4332A8427EDA9AB8	FASTA	599	68,024
	10 20 30 40 50 60 MWRCALLTSC SPSGISLRTG RWTWRHSWLD QICISFDEGK TTMNFIEAAL LIQGSACVYS 70 80 90 100 110 120 KKVEYLYSLV YQALDFISGK RRAKQLSLVQ EDGSKKTVNS ETPCETENEF LSLDDFPDSR 130 140 150 160 170 180 ANVDLKNDQA SSELLIIPLL PMALVAPDEV EKNSSPLYSC QGDILASRKD FRMNTCMPNP 190 200 210 220 230 240 RGCFMLDPVG MCPVEPVVPV EPYPMSRSQK DPEDAEEQPM EVSRNGSPVP VPDISQEPDG 250 260 270 280 290 300 PALSGGEEDA EDGAEPLEVA LEPAEPRTSQ QSAILPRRYM LRERQGAPEP ASRLQETPDP 310 320 330 340 350 360 WQSLDPFDSL ESKVFQKGKP YSVPPGVEEA PGQKRKRKGA TKLQDFHKWY LDAYAEHPDG 370 380 390 400 410 420 RRARRKGPTF ADMEVLYWKH VKEQLETLQK LRRRKINERW LPGAKQDLWP TEEDRLEESL 430 440 450 460 470 480 EDLGVADDFL EPEEYVEEPA GVMPEEAADL DAEAMPESLR YEELVRRNVE LFIATSQKFI 490 500 510 520 530 540 QETELSQRIR DWEDTIQPLL QEQEQHVPFD IHIYGDQLAS RFPQLNEWCP FSELVAGQPA 550 560 570 580 590 FEVCRSMLAS LQLANDYTVE ITQQPGLEAA VDTMSLRLLT HQRAHTRFQT YAAPSMAQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6J and NOD. Tissue: Cerebellum, Embryo, Kidney, Skin, Spleen and Thymus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
[3]	"A mutation in a chromosome condensin II subunit, kleisin beta, specifically disrupts T cell development." Gosling K.M., Makaroff L.E., Theodoratos A., Kim Y.-H., Whittle B., Rui L., Wu H., Hong N.A., Kennedy G.C., Fritz J.-A., Yates A.L., Goodnow C.C., Fahrer A.M. Proc. Natl. Acad. Sci. U.S.A. 104:12445-12450(2007) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING, VARIANT NESSY ASN-15.
[4]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-494, MASS SPECTROMETRY. Tissue: Embryonic kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK004160 mRNA. Translation: BAB23198.1. AK031135 mRNA. Translation: BAC27270.1. AK037175 mRNA. Translation: BAC29736.1. AK080447 mRNA. Translation: BAC37919.1. AK085584 mRNA. Translation: BAC39479.1. AK088294 mRNA. Translation: BAC40265.1. AK146642 mRNA. Translation: BAE27325.1. AK152158 mRNA. Translation: BAE30993.1. AK163958 mRNA. Translation: BAE37553.1. AK165250 mRNA. Translation: BAE38104.1. BC003900 mRNA. Translation: AAH03900.1. Different initiation.
IPI	IPI00403116. IPI00625515. IPI00856653.
RefSeq	NP_001108604.1. NM_001115132.2. NP_001258530.1. NM_001271601.1.
UniGene	Mm.143167.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q8BSP2.
Proteomic databases
PaxDb	Q8BSP2.
PRIDE	Q8BSP2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000074552; ENSMUSP00000074139; ENSMUSG00000008690. ENSMUST00000088717; ENSMUSP00000086095; ENSMUSG00000008690.
GeneID	52683.
KEGG	mmu:52683.
UCSC	uc007xgg.2. mouse. uc007xgh.2. mouse. uc007xgk.2. mouse.
Organism-specific databases
CTD	29781.
MGI	MGI:1289164. Ncaph2.
Phylogenomic databases
eggNOG	NOG238357.
GeneTree	ENSGT00390000014443.
HOVERGEN	HBG098083.
KO	K11490.
OMA	EVSVCRS.
OrthoDB	EOG408N7P.
Gene expression databases
ArrayExpress	Q8BSP2.
Bgee	Q8BSP2.
CleanEx	MM_NCAPH2.
Genevestigator	Q8BSP2.
Family and domain databases
InterPro	IPR009378. Condensin_II_H2-like. [Graphical view]
Pfam	PF06278. DUF1032. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NCAPH2. mouse.
NextBio	309335.
SOURCE	Search...

Entry information

Entry name

CNDH2_MOUSE

Accession

Primary (citable) accession number: Q8BSP2
Secondary accession number(s): Q3TNI4 , Q8C2N3, Q8C3K9, Q8C4X8, Q8CAZ3, Q99L21, Q9CT99

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program