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Protein

GRB2-associated-binding protein 3

Gene

Gab3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  • macrophage differentiation Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
GRB2-associated-binding protein 3
Alternative name(s):
GRB2-associated binder 3
Growth factor receptor bound protein 2-associated protein 3
Gene namesi
Name:Gab3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2387324. Gab3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 595595GRB2-associated-binding protein 3PRO_0000318940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei346 – 3461PhosphoserineBy similarity
Modified residuei480 – 4801PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residue(s) after macrophage colony-stimulating factor (M-CSF) receptor stimulation.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BSM5.
PRIDEiQ8BSM5.

PTM databases

iPTMnetiQ8BSM5.
PhosphoSiteiQ8BSM5.

Expressioni

Tissue specificityi

Highly expressed in spleen and thymus and weakly in brain, heart, lung, kidney, uterus, and embryonic stem cells. Also expressed in myeloid and macrophage cell lines.

Gene expression databases

BgeeiQ8BSM5.
ExpressionAtlasiQ8BSM5. baseline and differential.
GenevisibleiQ8BSM5. MM.

Interactioni

Subunit structurei

Interacts with PIK3R/p85, SHP2 and GRAP2/MONA. May interact with Grb2.2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041951.

Structurei

3D structure databases

ProteinModelPortaliQ8BSM5.
SMRiQ8BSM5. Positions 26-109.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 117113PHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi497 – 52024Glu-richAdd
BLAST

Sequence similaritiesi

Belongs to the GAB family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IEG0. Eukaryota.
ENOG4111RDE. LUCA.
GeneTreeiENSGT00510000046662.
HOVERGENiHBG107126.
InParanoidiQ8BSM5.
OMAiEFQNNFV.
OrthoDBiEOG7T4MJJ.
TreeFamiTF329487.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BSM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTGDTVCMG WLIKSPPERK LQRYAWRKRW FVLRRGRMSG NPDVLEYYRN
60 70 80 90 100
KHSNKPIRVI DLSECTVWKH AGPGFIRKEF QKNFVFIVKT TSRTFYLVAK
110 120 130 140 150
TEEEMQVWVH SISQVCNFSH LEDGADSMES LSHMPSSFQP SPASSLHTVH
160 170 180 190 200
VANSALLKDD RNTNSVVTEE TRRESEFLFL PDYLILSNCE TGRLHHASLP
210 220 230 240 250
TRCDSWSNSN HSLAQTSFDD VFLDGLQPFI SNNLVHPLHH GKVSQDFPSI
260 270 280 290 300
RPQASLIWNR EINGPSRNLM SSSPLLESSL NPTVHVEEKQ VSLPSGVKEL
310 320 330 340 350
NIMSNTPPPR PPKPSYLSEQ RQDQPLLTGH SSNKKPGYTM VPRRISLSGL
360 370 380 390 400
DHVGSWKGDV QSQSLRHRDK RLSLNLPCKF SPIYPTASPS AEDSYVPMSP
410 420 430 440 450
KGTASELRPH CSQDDYIPMS SSMLPELPAD LEPPPVNRNL KPQRKSRPPP
460 470 480 490 500
LDSRNLSTIQ EHTSLTRTYT VPCNRTSFLS PQRNGINCAR LFSTPSEEEE
510 520 530 540 550
EEEEEEEEEE EEEKYIQMEE YGTVSSLSRS ALSWTKKFSL DYLALDFNST
560 570 580 590
SPAPVQKKLL LSEEQRVDYV QVDEQKTQAL RSTKQEWTDE RQSKV
Length:595
Mass (Da):67,840
Last modified:March 1, 2003 - v1
Checksum:i59850B7B55276F30
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti161 – 1611R → G in AAL25824 (PubMed:11739737).Curated
Sequence conflicti306 – 3061T → I in AAL25824 (PubMed:11739737).Curated
Sequence conflicti586 – 5861E → A in AAL25824 (PubMed:11739737).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057988 mRNA. Translation: AAL25824.1.
AK031227 mRNA. Translation: BAC27308.1.
AL845304, AL808110 Genomic DNA. Translation: CAM16813.1.
AL808110, AL845304 Genomic DNA. Translation: CAM26483.1.
AJ748654 mRNA. Translation: CAG38688.1.
CCDSiCCDS30235.1.
RefSeqiNP_853615.1. NM_181584.4.
UniGeneiMm.267056.

Genome annotation databases

EnsembliENSMUST00000037374; ENSMUSP00000041951; ENSMUSG00000032750.
GeneIDi210710.
KEGGimmu:210710.
UCSCiuc009tpn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057988 mRNA. Translation: AAL25824.1.
AK031227 mRNA. Translation: BAC27308.1.
AL845304, AL808110 Genomic DNA. Translation: CAM16813.1.
AL808110, AL845304 Genomic DNA. Translation: CAM26483.1.
AJ748654 mRNA. Translation: CAG38688.1.
CCDSiCCDS30235.1.
RefSeqiNP_853615.1. NM_181584.4.
UniGeneiMm.267056.

3D structure databases

ProteinModelPortaliQ8BSM5.
SMRiQ8BSM5. Positions 26-109.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041951.

PTM databases

iPTMnetiQ8BSM5.
PhosphoSiteiQ8BSM5.

Proteomic databases

PaxDbiQ8BSM5.
PRIDEiQ8BSM5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037374; ENSMUSP00000041951; ENSMUSG00000032750.
GeneIDi210710.
KEGGimmu:210710.
UCSCiuc009tpn.2. mouse.

Organism-specific databases

CTDi139716.
MGIiMGI:2387324. Gab3.

Phylogenomic databases

eggNOGiENOG410IEG0. Eukaryota.
ENOG4111RDE. LUCA.
GeneTreeiENSGT00510000046662.
HOVERGENiHBG107126.
InParanoidiQ8BSM5.
OMAiEFQNNFV.
OrthoDBiEOG7T4MJJ.
TreeFamiTF329487.

Miscellaneous databases

PROiQ8BSM5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BSM5.
ExpressionAtlasiQ8BSM5. baseline and differential.
GenevisibleiQ8BSM5. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gab3, a new DOS/Gab family member, facilitates macrophage differentiation."
    Wolf I., Jenkins B.J., Liu Y., Seiffert M., Custodio J.M., Young P., Rohrschneider L.R.
    Mol. Cell. Biol. 22:231-244(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH PIK3R AND SHP2, INDUCTION BY M-CSF, PHOSPHORYLATION.
    Strain: BALB/cJ.
    Tissue: Spleen.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Forelimb.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The systematic functional characterisation of Xq28 genes prioritises candidate disease genes."
    Kolb-Kokocinski A., Mehrle A., Bechtel S., Simpson J.C., Kioschis P., Wiemann S., Wellenreuther R., Poustka A.
    BMC Genomics 7:29-29(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 330-579.
    Strain: NMRI.
    Tissue: Brain and Heart.
  5. "Induced expression and association of the Mona/Gads adapter and Gab3 scaffolding protein during monocyte/macrophage differentiation."
    Bourgin C., Bourette R.P., Arnaud S., Liu Y., Rohrschneider L.R., Mouchiroud G.
    Mol. Cell. Biol. 22:3744-3756(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRAP2.
  6. "Gab3-deficient mice exhibit normal development and hematopoiesis and are immunocompetent."
    Seiffert M., Custodio J.M., Wolf I., Harkey M., Liu Y., Blattman J.N., Greenberg P.D., Rohrschneider L.R.
    Mol. Cell. Biol. 23:2415-2424(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGAB3_MOUSE
AccessioniPrimary (citable) accession number: Q8BSM5
Secondary accession number(s): Q684I9, Q8VH53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2003
Last modified: July 6, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.