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Protein

ADP-ribosylation factor 2

Gene

Arf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTPBy similarity
Nucleotide bindingi67 – 715GTPBy similarity
Nucleotide bindingi126 – 1294GTPBy similarity

GO - Molecular functioni

  • GTP binding Source: MGI

GO - Biological processi

  • protein transport Source: MGI
  • small GTPase mediated signal transduction Source: InterPro
  • vesicle-mediated transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-ribosylation factor 2
Gene namesi
Name:Arf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:99595. Arf2.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedSequence Analysis
Chaini2 – 181180ADP-ribosylation factor 2PRO_0000207384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence Analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiQ8BSL7.
PaxDbiQ8BSL7.
PRIDEiQ8BSL7.

PTM databases

PhosphoSiteiQ8BSL7.

Expressioni

Gene expression databases

BgeeiQ8BSL7.
CleanExiMM_ARF2.
ExpressionAtlasiQ8BSL7. baseline and differential.
GenevisibleiQ8BSL7. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8BSL7. 1 interaction.
STRINGi10090.ENSMUSP00000051814.

Structurei

Secondary structure

1
181
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi18 – 236Combined sources
Helixi30 – 3910Combined sources
Beta strandi49 – 5810Combined sources
Beta strandi61 – 688Combined sources
Helixi72 – 8110Combined sources
Beta strandi87 – 937Combined sources
Helixi97 – 993Combined sources
Helixi100 – 11112Combined sources
Helixi114 – 1163Combined sources
Beta strandi120 – 1267Combined sources
Helixi136 – 1438Combined sources
Helixi145 – 1473Combined sources
Beta strandi153 – 1575Combined sources
Turni160 – 1634Combined sources
Helixi166 – 17813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J2JX-ray1.60A18-181[»]
ProteinModelPortaliQ8BSL7.
SMRiQ8BSL7. Positions 18-177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8BSL7.

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Arf family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiQ8BSL7.
KOiK06883.
OMAiNDNERVS.
OrthoDBiEOG77WWDV.
PhylomeDBiQ8BSL7.
TreeFamiTF300808.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BSL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNVFEKLFK SLFGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG
60 70 80 90 100
FNVETVEYKN ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV
110 120 130 140 150
NEAREELTRM LAEDELRDAV LLVFVNKQDL PNAMNAAEIT DKLGLHSLRQ
160 170 180
RNWYIQATCA TSGDGLYEGL DWLSNQLKNQ K
Length:181
Mass (Da):20,746
Last modified:August 16, 2004 - v2
Checksum:i95BE17A962B83016
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 552ET → GH in AAH10487 (PubMed:15489334).Curated
Sequence conflicti154 – 1541Y → N in BAC27325 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87899 mRNA. Translation: BAA13491.1.
AK031259 mRNA. Translation: BAC27325.1.
AK042977 mRNA. Translation: BAC31426.1.
AK053123 mRNA. Translation: BAC35273.1.
AK077591 mRNA. Translation: BAC36882.1.
AK167133 mRNA. Translation: BAE39279.1.
AK167601 mRNA. Translation: BAE39658.1.
AK167653 mRNA. Translation: BAE39704.1.
AK169094 mRNA. Translation: BAE40877.1.
AK169412 mRNA. Translation: BAE41158.1.
BC010487 mRNA. Translation: AAH10487.1.
CCDSiCCDS25525.1.
PIRiJC4946.
RefSeqiNP_001291503.1. NM_001304574.1.
NP_031503.1. NM_007477.5.
UniGeneiMm.5061.

Genome annotation databases

EnsembliENSMUST00000057921; ENSMUSP00000051814; ENSMUSG00000062421.
ENSMUST00000063347; ENSMUSP00000102622; ENSMUSG00000062421.
GeneIDi11841.
KEGGimmu:11841.
UCSCiuc007lvx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87899 mRNA. Translation: BAA13491.1.
AK031259 mRNA. Translation: BAC27325.1.
AK042977 mRNA. Translation: BAC31426.1.
AK053123 mRNA. Translation: BAC35273.1.
AK077591 mRNA. Translation: BAC36882.1.
AK167133 mRNA. Translation: BAE39279.1.
AK167601 mRNA. Translation: BAE39658.1.
AK167653 mRNA. Translation: BAE39704.1.
AK169094 mRNA. Translation: BAE40877.1.
AK169412 mRNA. Translation: BAE41158.1.
BC010487 mRNA. Translation: AAH10487.1.
CCDSiCCDS25525.1.
PIRiJC4946.
RefSeqiNP_001291503.1. NM_001304574.1.
NP_031503.1. NM_007477.5.
UniGeneiMm.5061.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J2JX-ray1.60A18-181[»]
ProteinModelPortaliQ8BSL7.
SMRiQ8BSL7. Positions 18-177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BSL7. 1 interaction.
STRINGi10090.ENSMUSP00000051814.

PTM databases

PhosphoSiteiQ8BSL7.

Proteomic databases

MaxQBiQ8BSL7.
PaxDbiQ8BSL7.
PRIDEiQ8BSL7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057921; ENSMUSP00000051814; ENSMUSG00000062421.
ENSMUST00000063347; ENSMUSP00000102622; ENSMUSG00000062421.
GeneIDi11841.
KEGGimmu:11841.
UCSCiuc007lvx.2. mouse.

Organism-specific databases

CTDi11841.
MGIiMGI:99595. Arf2.

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00780000121855.
HOGENOMiHOG000163691.
HOVERGENiHBG002073.
InParanoidiQ8BSL7.
KOiK06883.
OMAiNDNERVS.
OrthoDBiEOG77WWDV.
PhylomeDBiQ8BSL7.
TreeFamiTF300808.

Miscellaneous databases

ChiTaRSiArf2. mouse.
EvolutionaryTraceiQ8BSL7.
NextBioi20806307.
PROiQ8BSL7.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BSL7.
CleanExiMM_ARF2.
ExpressionAtlasiQ8BSL7. baseline and differential.
GenevisibleiQ8BSL7. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR024156. Small_GTPase_ARF.
IPR006689. Small_GTPase_ARF/SAR.
[Graphical view]
PfamiPF00025. Arf. 1 hit.
[Graphical view]
PRINTSiPR00328. SAR1GTPBP.
SMARTiSM00177. ARF. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51417. ARF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and intracellular localization of mouse ADP-ribosylation factors type 1 to type 6 (ARF1-ARF6)."
    Hosaka M., Toda K., Takatsu H., Torii S., Murakami K., Nakayama K.
    J. Biochem. 120:813-819(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Amnion, Cerebellum, Embryo, Forelimb, Heart, Lung and Placenta.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  4. Lubec G., Klug S.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 39-59 AND 80-97, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.

Entry informationi

Entry nameiARF2_MOUSE
AccessioniPrimary (citable) accession number: Q8BSL7
Secondary accession number(s): P10947
, P16500, Q3TJ37, Q91VR9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: July 22, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.