ID   GPHR_MOUSE              Reviewed;         455 AA.
AC   Q8BS95; Q9D455;
DT   07-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 2.
DT   24-JAN-2024, entry version 119.
DE   RecName: Full=Golgi pH regulator;
DE   AltName: Full=Protein GPR89;
GN   Name=Gpr89 {ECO:0000312|MGI:MGI:1914799};
GN   Synonyms=Gphr, Gpr89a {ECO:0000312|MGI:MGI:1914799};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Embryo, and Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Pancreas;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH RABL3, AND
RP   MUTAGENESIS OF 273-GLU--MET-303.
RX   PubMed=32220963; DOI=10.1073/pnas.2000703117;
RA   Zhong X., Su L., Yang Y., Nair-Gill E., Tang M., Anderton P., Li X.,
RA   Wang J., Zhan X., Tomchick D.R., Brautigam C.A., Moresco E.M.Y., Choi J.H.,
RA   Beutler B.;
RT   "Genetic and structural studies of RABL3 reveal an essential role in
RT   lymphoid development and function.";
RL   Proc. Natl. Acad. Sci. U.S.A. 117:8563-8572(2020).
CC   -!- FUNCTION: Voltage dependent anion channel required for acidification
CC       and functions of the Golgi apparatus that may function in counter-ion
CC       conductance (By similarity). Plays a role in lymphocyte development,
CC       probably by acting as a RABL3 effector in hematopoietic cells
CC       (PubMed:32220963). {ECO:0000250|UniProtKB:B2ZXD5,
CC       ECO:0000269|PubMed:32220963}.
CC   -!- SUBUNIT: Homotrimer (By similarity). Interacts with RABL3; the
CC       interaction stabilizes GPR89 (PubMed:32220963).
CC       {ECO:0000250|UniProtKB:B7ZAQ6, ECO:0000269|PubMed:32220963}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000250|UniProtKB:B2ZXD5}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the Golgi pH regulator (TC 1.A.38) family.
CC       {ECO:0000305}.
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DR   EMBL; AK034895; BAC28872.1; -; mRNA.
DR   EMBL; AK016787; BAB30430.1; -; mRNA.
DR   EMBL; BC010729; AAH10729.1; -; mRNA.
DR   CCDS; CCDS17650.1; -.
DR   RefSeq; NP_080505.1; NM_026229.4.
DR   AlphaFoldDB; Q8BS95; -.
DR   SMR; Q8BS95; -.
DR   BioGRID; 212268; 2.
DR   STRING; 10090.ENSMUSP00000029738; -.
DR   GlyCosmos; Q8BS95; 1 site, No reported glycans.
DR   GlyGen; Q8BS95; 1 site.
DR   iPTMnet; Q8BS95; -.
DR   PhosphoSitePlus; Q8BS95; -.
DR   SwissPalm; Q8BS95; -.
DR   EPD; Q8BS95; -.
DR   MaxQB; Q8BS95; -.
DR   PaxDb; 10090-ENSMUSP00000029738; -.
DR   PeptideAtlas; Q8BS95; -.
DR   ProteomicsDB; 271266; -.
DR   Pumba; Q8BS95; -.
DR   DNASU; 67549; -.
DR   Ensembl; ENSMUST00000029738.14; ENSMUSP00000029738.8; ENSMUSG00000028096.14.
DR   GeneID; 67549; -.
DR   KEGG; mmu:67549; -.
DR   UCSC; uc008qom.1; mouse.
DR   AGR; MGI:1914799; -.
DR   CTD; 67549; -.
DR   MGI; MGI:1914799; Gpr89.
DR   VEuPathDB; HostDB:ENSMUSG00000028096; -.
DR   eggNOG; KOG2417; Eukaryota.
DR   GeneTree; ENSGT00390000000684; -.
DR   HOGENOM; CLU_030540_1_0_1; -.
DR   InParanoid; Q8BS95; -.
DR   OMA; FSVYCVY; -.
DR   OrthoDB; 90523at2759; -.
DR   PhylomeDB; Q8BS95; -.
DR   TreeFam; TF313484; -.
DR   BioGRID-ORCS; 67549; 7 hits in 81 CRISPR screens.
DR   ChiTaRS; Gpr89; mouse.
DR   PRO; PR:Q8BS95; -.
DR   Proteomes; UP000000589; Chromosome 3.
DR   RNAct; Q8BS95; Protein.
DR   Bgee; ENSMUSG00000028096; Expressed in small intestine Peyer's patch and 266 other cell types or tissues.
DR   ExpressionAtlas; Q8BS95; baseline and differential.
DR   GO; GO:0032580; C:Golgi cisterna membrane; ISS:UniProtKB.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030660; C:Golgi-associated vesicle membrane; ISS:UniProtKB.
DR   GO; GO:0034702; C:monoatomic ion channel complex; IEA:UniProtKB-KW.
DR   GO; GO:0008308; F:voltage-gated monoatomic anion channel activity; ISS:UniProtKB.
DR   GO; GO:0051452; P:intracellular pH reduction; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0043588; P:skin development; IMP:MGI.
DR   GO; GO:0030217; P:T cell differentiation; IMP:UniProtKB.
DR   InterPro; IPR025969; ABA_GPCR_dom.
DR   InterPro; IPR022535; Golgi_pH-regulator_cons_dom.
DR   InterPro; IPR015672; GPHR/GTG.
DR   PANTHER; PTHR15948; G-PROTEIN COUPLED RECEPTOR 89-RELATED; 1.
DR   PANTHER; PTHR15948:SF0; GOLGI PH REGULATOR A-RELATED; 1.
DR   Pfam; PF12430; ABA_GPCR; 1.
DR   Pfam; PF12537; GPHR_N; 1.
DR   Genevisible; Q8BS95; MM.
PE   1: Evidence at protein level;
KW   Glycoprotein; Golgi apparatus; Ion channel; Ion transport; Membrane;
KW   Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport; Voltage-gated channel.
FT   CHAIN           1..455
FT                   /note="Golgi pH regulator"
FT                   /id="PRO_0000223261"
FT   TRANSMEM        5..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        114..134
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        288..308
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        343..363
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        378..398
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        425..445
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        180
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         273..303
FT                   /note="Missing: In explorer; no obvious external
FT                   deformities but mutants are 40% smaller than wild-type
FT                   litter mates and show reduced frequency of CD3+ T cells,
FT                   CD4+ T cells and CD8+ T cells which all display elevated
FT                   CD44 expression."
FT                   /evidence="ECO:0000269|PubMed:32220963"
FT   CONFLICT        243
FT                   /note="N -> H (in Ref. 1; BAC28872)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   455 AA;  52735 MW;  CAE9B93C64262252 CRC64;
     MSFLIDSSIM ITSQILFFGF GWLFFMRQLF KDYEVRQYVV QVIFSVTFAF SCTMFELIIF
     EILGVLNSSS RYFHWKLNLC VILLILVFMV PFYIGYFIVS NIQLLHKQRL LFSCLLWLTF
     MYFFWKLGDP FPILSPKHGI LSIEQLISRV GVIGVTLMAL LSGFGAVNCP YTYMSYFLRN
     VTDTDILALE RRLLQTMDMI ISKKKRMAVA RRTMFQRGDV QNKPSGLWGM LKSVTASAPG
     SENLTLIQQE VDALEELSRQ LFLETADLYA TKERIEYSKT FKGKYFNFLG YFFSIYCVWK
     IFMATINIVL DRVGKTDPVT RGIEITVNYL GIQFDVKFWS QHISFILVGI IIVTSIRGLL
     ITLTKFFYAI SSSKSSNVIV LLLAQIMGMY FVSSVLLIRM SMPPEYRTII TEVLGELQFN
     FYHRWFDVIF LVSALSSILF LYLAHKQAPE KHMAP
//