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Protein

Synaptic vesicular amine transporter

Gene

Slc18a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • cellular response to ammonium ion Source: Ensembl
  • cellular response to drug Source: Ensembl
  • death Source: MGI
  • endocytic recycling Source: Ensembl
  • glucose homeostasis Source: Ensembl
  • insulin secretion Source: Ensembl
  • locomotory behavior Source: MGI
  • negative regulation of neurotransmitter transport Source: Ensembl
  • neurotransmitter transport Source: UniProtKB-KW
  • post-embryonic development Source: MGI
  • response to amphetamine Source: MGI
  • response to cocaine Source: Ensembl
  • response to corticosterone Source: Ensembl
  • response to herbicide Source: Ensembl
  • response to starvation Source: Ensembl
  • response to toxic substance Source: MGI
  • response to zinc ion Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiREACT_287707. Serotonin Neurotransmitter Release Cycle.
REACT_307956. Na+/Cl- dependent neurotransmitter transporters.
REACT_324353. Dopamine Neurotransmitter Release Cycle.
REACT_325189. Norepinephrine Neurotransmitter Release Cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name:
VAT2
Gene namesi
Name:Slc18a2
Synonyms:Vmat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:106677. Slc18a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei21 – 4121HelicalSequence AnalysisAdd
BLAST
Topological domaini42 – 13291Lumenal, vesicleSequence AnalysisAdd
BLAST
Transmembranei133 – 15321HelicalSequence AnalysisAdd
BLAST
Topological domaini154 – 1629CytoplasmicSequence Analysis
Transmembranei163 – 18321HelicalSequence AnalysisAdd
BLAST
Topological domaini184 – 1929Lumenal, vesicleSequence Analysis
Transmembranei193 – 21321HelicalSequence AnalysisAdd
BLAST
Topological domaini214 – 2229CytoplasmicSequence Analysis
Transmembranei223 – 24523HelicalSequence AnalysisAdd
BLAST
Topological domaini246 – 2516Lumenal, vesicleSequence Analysis
Transmembranei252 – 27423HelicalSequence AnalysisAdd
BLAST
Topological domaini275 – 29420CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei295 – 31420HelicalSequence AnalysisAdd
BLAST
Topological domaini315 – 33117Lumenal, vesicleSequence AnalysisAdd
BLAST
Transmembranei332 – 35524HelicalSequence AnalysisAdd
BLAST
Topological domaini356 – 3605CytoplasmicSequence Analysis
Transmembranei361 – 38121HelicalSequence AnalysisAdd
BLAST
Topological domaini382 – 39211Lumenal, vesicleSequence AnalysisAdd
BLAST
Transmembranei393 – 41321HelicalSequence AnalysisAdd
BLAST
Topological domaini414 – 4174CytoplasmicSequence Analysis
Transmembranei418 – 43821HelicalSequence AnalysisAdd
BLAST
Topological domaini439 – 4435Lumenal, vesicleSequence Analysis
Transmembranei444 – 46522HelicalSequence AnalysisAdd
BLAST
Topological domaini466 – 51752CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Synaptic vesicular amine transporterPRO_0000127515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi120 ↔ 327By similarity
Modified residuei514 – 5141Phosphoserine; by CK2By similarity
Modified residuei516 – 5161Phosphoserine; by CK2By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8BRU6.
PaxDbiQ8BRU6.
PRIDEiQ8BRU6.

PTM databases

PhosphoSiteiQ8BRU6.

Expressioni

Gene expression databases

BgeeiQ8BRU6.
GenevestigatoriQ8BRU6.

Interactioni

Subunit structurei

Interacts with SLC6A3.1 Publication

Protein-protein interaction databases

MINTiMINT-577047.
STRINGi10090.ENSMUSP00000026084.

Structurei

3D structure databases

ProteinModelPortaliQ8BRU6.
SMRiQ8BRU6. Positions 132-446.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG255549.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ8BRU6.
KOiK08155.
OMAiCPKEDKD.
OrthoDBiEOG79CXZ7.
PhylomeDBiQ8BRU6.
TreeFamiTF313494.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BRU6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS
60 70 80 90 100
IKHEKNTTEI QTARPALTAS TSESFHSIFS YYNNSTVFTG NATGGLPGGE
110 120 130 140 150
SPKATTTQHT VTNTTVPPDC PSEDKDLLNE NVQVGLLFAS KATVQLLTNP
160 170 180 190 200
FIGLLTNRIG YPIPMFAGFC IMFISTVMFA FSSSYAFLLI ARSLQGIGSS
210 220 230 240 250
CSSVAGMGML ASVYTDDEER GNAMGIALGG LAMGVLVGPP FGSVLYEFVG
260 270 280 290 300
KTAPFLVLAA LVLLDGAIQL FVLQPSRVQP ESQKGTPLTT LLKDPYILIA
310 320 330 340 350
AGSICFANMG IAMLEPALPI WMMETMCSRK WQLGVAFLPA SISYLIGTNI
360 370 380 390 400
FGILAHKMGR WLCALLGMIV VGISILCIPF AKNIYGLIAP NFGVGFAIGM
410 420 430 440 450
VDSSMMPIMG YLVDLRHVSV YGSVYAIADV AFCMGYAIGP SAGGAIAKAI
460 470 480 490 500
GFPWLMTIIG IIDIVFAPLC FFLRSPPAKE EKMAILMDHN CPIKTKMYTQ
510
NNVQPYPVGD DEESESD
Length:517
Mass (Da):55,754
Last modified:March 1, 2003 - v1
Checksum:i97B40B65DE3DDBEE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti150 – 1501P → T in BAC28501 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ555564 mRNA. Translation: CAD88262.1.
AK033871 mRNA. Translation: BAC28501.1.
AK041274 mRNA. Translation: BAC30887.1.
CCDSiCCDS29935.1.
RefSeqiNP_766111.1. NM_172523.3.
UniGeneiMm.268797.
Mm.361919.

Genome annotation databases

EnsembliENSMUST00000026084; ENSMUSP00000026084; ENSMUSG00000025094.
GeneIDi214084.
KEGGimmu:214084.
UCSCiuc008ibi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ555564 mRNA. Translation: CAD88262.1.
AK033871 mRNA. Translation: BAC28501.1.
AK041274 mRNA. Translation: BAC30887.1.
CCDSiCCDS29935.1.
RefSeqiNP_766111.1. NM_172523.3.
UniGeneiMm.268797.
Mm.361919.

3D structure databases

ProteinModelPortaliQ8BRU6.
SMRiQ8BRU6. Positions 132-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-577047.
STRINGi10090.ENSMUSP00000026084.

PTM databases

PhosphoSiteiQ8BRU6.

Proteomic databases

MaxQBiQ8BRU6.
PaxDbiQ8BRU6.
PRIDEiQ8BRU6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026084; ENSMUSP00000026084; ENSMUSG00000025094.
GeneIDi214084.
KEGGimmu:214084.
UCSCiuc008ibi.2. mouse.

Organism-specific databases

CTDi6571.
MGIiMGI:106677. Slc18a2.

Phylogenomic databases

eggNOGiNOG255549.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ8BRU6.
KOiK08155.
OMAiCPKEDKD.
OrthoDBiEOG79CXZ7.
PhylomeDBiQ8BRU6.
TreeFamiTF313494.

Enzyme and pathway databases

ReactomeiREACT_287707. Serotonin Neurotransmitter Release Cycle.
REACT_307956. Na+/Cl- dependent neurotransmitter transporters.
REACT_324353. Dopamine Neurotransmitter Release Cycle.
REACT_325189. Norepinephrine Neurotransmitter Release Cycle.

Miscellaneous databases

NextBioi374178.
PROiQ8BRU6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BRU6.
GenevestigatoriQ8BRU6.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and pharmacological characterization of the mouse vesicular monoamine transporter 2 (mVMAT2)."
    Gilsbach R., Bonisch H., Bruess M.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Aorta, Diencephalon and Vein.
  3. "Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3."
    Egana L.A., Cuevas R.A., Baust T.B., Parra L.A., Leak R.K., Hochendoner S., Pena K., Quiroz M., Hong W.C., Dorostkar M.M., Janz R., Sitte H.H., Torres G.E.
    J. Neurosci. 29:4592-4604(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLC6A3.

Entry informationi

Entry nameiVMAT2_MOUSE
AccessioniPrimary (citable) accession number: Q8BRU6
Secondary accession number(s): Q8CC55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: April 1, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.