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Q8BRU6 (VMAT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name=VAT2
Gene names
Name:Slc18a2
Synonyms:Vmat2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis By similarity.

Subunit structure

Interacts with SLC6A3. Ref.3

Subcellular location

Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the major facilitator superfamily. Vesicular transporter family.

Ontologies

Keywords
   Biological processNeurotransmitter transport
Transport
   Cellular componentCytoplasmic vesicle
Membrane
   DomainTransmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processaging

Inferred from electronic annotation. Source: Ensembl

cellular response to ammonium ion

Inferred from electronic annotation. Source: Ensembl

cellular response to drug

Inferred from electronic annotation. Source: Ensembl

death

Inferred from mutant phenotype PubMed 9275230. Source: MGI

endocytic recycling

Inferred from electronic annotation. Source: Ensembl

glucose homeostasis

Inferred from electronic annotation. Source: Ensembl

insulin secretion

Inferred from electronic annotation. Source: Ensembl

locomotory behavior

Inferred from mutant phenotype PubMed 9275230. Source: MGI

negative regulation of neurotransmitter transport

Inferred from electronic annotation. Source: Ensembl

neurotransmitter transport

Inferred from electronic annotation. Source: UniProtKB-KW

post-embryonic development

Inferred from mutant phenotype PubMed 9275230. Source: MGI

response to amphetamine

Inferred from mutant phenotype PubMed 9275230. Source: MGI

response to cocaine

Inferred from electronic annotation. Source: Ensembl

response to corticosterone

Inferred from electronic annotation. Source: Ensembl

response to herbicide

Inferred from electronic annotation. Source: Ensembl

response to starvation

Inferred from electronic annotation. Source: Ensembl

response to toxic substance

Inferred from mutant phenotype PubMed 9275230. Source: MGI

response to zinc ion

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentdense core granule

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

synaptic vesicle membrane

Inferred from electronic annotation. Source: Ensembl

terminal bouton

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionamine transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

drug binding

Inferred from electronic annotation. Source: Ensembl

drug transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

serotonin transmembrane transporter activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 517517Synaptic vesicular amine transporter
PRO_0000127515

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121Helical; Potential
Topological domain42 – 13291Lumenal, vesicle Potential
Transmembrane133 – 15321Helical; Potential
Topological domain154 – 1629Cytoplasmic Potential
Transmembrane163 – 18321Helical; Potential
Topological domain184 – 1929Lumenal, vesicle Potential
Transmembrane193 – 21321Helical; Potential
Topological domain214 – 2229Cytoplasmic Potential
Transmembrane223 – 24523Helical; Potential
Topological domain246 – 2516Lumenal, vesicle Potential
Transmembrane252 – 27423Helical; Potential
Topological domain275 – 29420Cytoplasmic Potential
Transmembrane295 – 31420Helical; Potential
Topological domain315 – 33117Lumenal, vesicle Potential
Transmembrane332 – 35524Helical; Potential
Topological domain356 – 3605Cytoplasmic Potential
Transmembrane361 – 38121Helical; Potential
Topological domain382 – 39211Lumenal, vesicle Potential
Transmembrane393 – 41321Helical; Potential
Topological domain414 – 4174Cytoplasmic Potential
Transmembrane418 – 43821Helical; Potential
Topological domain439 – 4435Lumenal, vesicle Potential
Transmembrane444 – 46522Helical; Potential
Topological domain466 – 51752Cytoplasmic Potential

Amino acid modifications

Modified residue5141Phosphoserine; by CK2 By similarity
Modified residue5161Phosphoserine; by CK2 By similarity
Glycosylation561N-linked (GlcNAc...) Potential
Glycosylation831N-linked (GlcNAc...) Potential
Glycosylation841N-linked (GlcNAc...) Potential
Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation1131N-linked (GlcNAc...) Potential
Disulfide bond120 ↔ 327 By similarity

Experimental info

Sequence conflict1501P → T in BAC28501. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8BRU6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 97B40B65DE3DDBEE

FASTA51755,754
        10         20         30         40         50         60 
MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS IKHEKNTTEI 

        70         80         90        100        110        120 
QTARPALTAS TSESFHSIFS YYNNSTVFTG NATGGLPGGE SPKATTTQHT VTNTTVPPDC 

       130        140        150        160        170        180 
PSEDKDLLNE NVQVGLLFAS KATVQLLTNP FIGLLTNRIG YPIPMFAGFC IMFISTVMFA 

       190        200        210        220        230        240 
FSSSYAFLLI ARSLQGIGSS CSSVAGMGML ASVYTDDEER GNAMGIALGG LAMGVLVGPP 

       250        260        270        280        290        300 
FGSVLYEFVG KTAPFLVLAA LVLLDGAIQL FVLQPSRVQP ESQKGTPLTT LLKDPYILIA 

       310        320        330        340        350        360 
AGSICFANMG IAMLEPALPI WMMETMCSRK WQLGVAFLPA SISYLIGTNI FGILAHKMGR 

       370        380        390        400        410        420 
WLCALLGMIV VGISILCIPF AKNIYGLIAP NFGVGFAIGM VDSSMMPIMG YLVDLRHVSV 

       430        440        450        460        470        480 
YGSVYAIADV AFCMGYAIGP SAGGAIAKAI GFPWLMTIIG IIDIVFAPLC FFLRSPPAKE 

       490        500        510 
EKMAILMDHN CPIKTKMYTQ NNVQPYPVGD DEESESD 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and pharmacological characterization of the mouse vesicular monoamine transporter 2 (mVMAT2)."
Gilsbach R., Bonisch H., Bruess M.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Aorta, Diencephalon and Vein.
[3]"Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3."
Egana L.A., Cuevas R.A., Baust T.B., Parra L.A., Leak R.K., Hochendoner S., Pena K., Quiroz M., Hong W.C., Dorostkar M.M., Janz R., Sitte H.H., Torres G.E.
J. Neurosci. 29:4592-4604(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SLC6A3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ555564 mRNA. Translation: CAD88262.1.
AK033871 mRNA. Translation: BAC28501.1.
AK041274 mRNA. Translation: BAC30887.1.
RefSeqNP_766111.1. NM_172523.3.
UniGeneMm.268797.
Mm.361919.

3D structure databases

ProteinModelPortalQ8BRU6.
SMRQ8BRU6. Positions 132-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-577047.
STRING10090.ENSMUSP00000026084.

PTM databases

PhosphoSiteQ8BRU6.

Proteomic databases

PaxDbQ8BRU6.
PRIDEQ8BRU6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026084; ENSMUSP00000026084; ENSMUSG00000025094.
GeneID214084.
KEGGmmu:214084.
UCSCuc008ibi.2. mouse.

Organism-specific databases

CTD6571.
MGIMGI:106677. Slc18a2.

Phylogenomic databases

eggNOGNOG255549.
GeneTreeENSGT00390000003851.
HOGENOMHOG000018545.
HOVERGENHBG055082.
InParanoidQ8BRU6.
KOK08155.
OMATNRIGYQ.
OrthoDBEOG79CXZ7.
PhylomeDBQ8BRU6.
TreeFamTF313494.

Gene expression databases

BgeeQ8BRU6.
GenevestigatorQ8BRU6.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 2 hits.
TIGRFAMsTIGR00880. 2_A_01_02. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio374178.
PROQ8BRU6.
SOURCESearch...

Entry information

Entry nameVMAT2_MOUSE
AccessionPrimary (citable) accession number: Q8BRU6
Secondary accession number(s): Q8CC55
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot