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Protein

Synaptic vesicular amine transporter

Gene

Slc18a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • aminergic neurotransmitter loading into synaptic vesicle Source: GO_Central
  • chemical synaptic transmission Source: GO_Central
  • locomotory behavior Source: MGI
  • post-embryonic development Source: MGI
  • response to amphetamine Source: MGI
  • response to toxic substance Source: MGI
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-181429. Serotonin Neurotransmitter Release Cycle.
R-MMU-181430. Norepinephrine Neurotransmitter Release Cycle.
R-MMU-212676. Dopamine Neurotransmitter Release Cycle.
R-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name:
VAT2
Gene namesi
Name:Slc18a2
Synonyms:Vmat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:106677. Slc18a2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 20CytoplasmicSequence analysisAdd BLAST20
Transmembranei21 – 41HelicalSequence analysisAdd BLAST21
Topological domaini42 – 132Lumenal, vesicleSequence analysisAdd BLAST91
Transmembranei133 – 153HelicalSequence analysisAdd BLAST21
Topological domaini154 – 162CytoplasmicSequence analysis9
Transmembranei163 – 183HelicalSequence analysisAdd BLAST21
Topological domaini184 – 192Lumenal, vesicleSequence analysis9
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Topological domaini214 – 222CytoplasmicSequence analysis9
Transmembranei223 – 245HelicalSequence analysisAdd BLAST23
Topological domaini246 – 251Lumenal, vesicleSequence analysis6
Transmembranei252 – 274HelicalSequence analysisAdd BLAST23
Topological domaini275 – 294CytoplasmicSequence analysisAdd BLAST20
Transmembranei295 – 314HelicalSequence analysisAdd BLAST20
Topological domaini315 – 331Lumenal, vesicleSequence analysisAdd BLAST17
Transmembranei332 – 355HelicalSequence analysisAdd BLAST24
Topological domaini356 – 360CytoplasmicSequence analysis5
Transmembranei361 – 381HelicalSequence analysisAdd BLAST21
Topological domaini382 – 392Lumenal, vesicleSequence analysisAdd BLAST11
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 417CytoplasmicSequence analysis4
Transmembranei418 – 438HelicalSequence analysisAdd BLAST21
Topological domaini439 – 443Lumenal, vesicleSequence analysis5
Transmembranei444 – 465HelicalSequence analysisAdd BLAST22
Topological domaini466 – 517CytoplasmicSequence analysisAdd BLAST52

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001275151 – 517Synaptic vesicular amine transporterAdd BLAST517

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi56N-linked (GlcNAc...)Sequence analysis1
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi91N-linked (GlcNAc...)Sequence analysis1
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi120 ↔ 327By similarity
Modified residuei514Phosphoserine; by CK2By similarity1
Modified residuei516Phosphoserine; by CK2By similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8BRU6.
MaxQBiQ8BRU6.
PaxDbiQ8BRU6.
PRIDEiQ8BRU6.

PTM databases

iPTMnetiQ8BRU6.
PhosphoSitePlusiQ8BRU6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025094.
GenevisibleiQ8BRU6. MM.

Interactioni

Subunit structurei

Interacts with SLC6A3.1 Publication

Protein-protein interaction databases

MINTiMINT-577047.
STRINGi10090.ENSMUSP00000026084.

Structurei

3D structure databases

ProteinModelPortaliQ8BRU6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3764. Eukaryota.
ENOG410XW69. LUCA.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ8BRU6.
KOiK08155.
OMAiQKAETEP.
OrthoDBiEOG091G0534.
PhylomeDBiQ8BRU6.
TreeFamiTF313494.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BRU6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS
60 70 80 90 100
IKHEKNTTEI QTARPALTAS TSESFHSIFS YYNNSTVFTG NATGGLPGGE
110 120 130 140 150
SPKATTTQHT VTNTTVPPDC PSEDKDLLNE NVQVGLLFAS KATVQLLTNP
160 170 180 190 200
FIGLLTNRIG YPIPMFAGFC IMFISTVMFA FSSSYAFLLI ARSLQGIGSS
210 220 230 240 250
CSSVAGMGML ASVYTDDEER GNAMGIALGG LAMGVLVGPP FGSVLYEFVG
260 270 280 290 300
KTAPFLVLAA LVLLDGAIQL FVLQPSRVQP ESQKGTPLTT LLKDPYILIA
310 320 330 340 350
AGSICFANMG IAMLEPALPI WMMETMCSRK WQLGVAFLPA SISYLIGTNI
360 370 380 390 400
FGILAHKMGR WLCALLGMIV VGISILCIPF AKNIYGLIAP NFGVGFAIGM
410 420 430 440 450
VDSSMMPIMG YLVDLRHVSV YGSVYAIADV AFCMGYAIGP SAGGAIAKAI
460 470 480 490 500
GFPWLMTIIG IIDIVFAPLC FFLRSPPAKE EKMAILMDHN CPIKTKMYTQ
510
NNVQPYPVGD DEESESD
Length:517
Mass (Da):55,754
Last modified:March 1, 2003 - v1
Checksum:i97B40B65DE3DDBEE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150P → T in BAC28501 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ555564 mRNA. Translation: CAD88262.1.
AK033871 mRNA. Translation: BAC28501.1.
AK041274 mRNA. Translation: BAC30887.1.
CCDSiCCDS29935.1.
RefSeqiNP_766111.1. NM_172523.3.
UniGeneiMm.268797.
Mm.361919.

Genome annotation databases

EnsembliENSMUST00000026084; ENSMUSP00000026084; ENSMUSG00000025094.
GeneIDi214084.
KEGGimmu:214084.
UCSCiuc008ibi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ555564 mRNA. Translation: CAD88262.1.
AK033871 mRNA. Translation: BAC28501.1.
AK041274 mRNA. Translation: BAC30887.1.
CCDSiCCDS29935.1.
RefSeqiNP_766111.1. NM_172523.3.
UniGeneiMm.268797.
Mm.361919.

3D structure databases

ProteinModelPortaliQ8BRU6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-577047.
STRINGi10090.ENSMUSP00000026084.

PTM databases

iPTMnetiQ8BRU6.
PhosphoSitePlusiQ8BRU6.

Proteomic databases

EPDiQ8BRU6.
MaxQBiQ8BRU6.
PaxDbiQ8BRU6.
PRIDEiQ8BRU6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026084; ENSMUSP00000026084; ENSMUSG00000025094.
GeneIDi214084.
KEGGimmu:214084.
UCSCiuc008ibi.2. mouse.

Organism-specific databases

CTDi6571.
MGIiMGI:106677. Slc18a2.

Phylogenomic databases

eggNOGiKOG3764. Eukaryota.
ENOG410XW69. LUCA.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ8BRU6.
KOiK08155.
OMAiQKAETEP.
OrthoDBiEOG091G0534.
PhylomeDBiQ8BRU6.
TreeFamiTF313494.

Enzyme and pathway databases

ReactomeiR-MMU-181429. Serotonin Neurotransmitter Release Cycle.
R-MMU-181430. Norepinephrine Neurotransmitter Release Cycle.
R-MMU-212676. Dopamine Neurotransmitter Release Cycle.
R-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

PROiQ8BRU6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025094.
GenevisibleiQ8BRU6. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVMAT2_MOUSE
AccessioniPrimary (citable) accession number: Q8BRU6
Secondary accession number(s): Q8CC55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.