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Q8BRU6

- VMAT2_MOUSE

UniProt

Q8BRU6 - VMAT2_MOUSE

Protein

Synaptic vesicular amine transporter

Gene

Slc18a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis By similarity.By similarity

    GO - Molecular functioni

    1. amine transmembrane transporter activity Source: Ensembl
    2. drug binding Source: Ensembl
    3. drug transmembrane transporter activity Source: InterPro
    4. protein binding Source: UniProtKB
    5. serotonin transmembrane transporter activity Source: Ensembl

    GO - Biological processi

    1. aging Source: Ensembl
    2. cellular response to ammonium ion Source: Ensembl
    3. cellular response to drug Source: Ensembl
    4. death Source: MGI
    5. endocytic recycling Source: Ensembl
    6. glucose homeostasis Source: Ensembl
    7. insulin secretion Source: Ensembl
    8. locomotory behavior Source: MGI
    9. negative regulation of neurotransmitter transport Source: Ensembl
    10. neurotransmitter transport Source: UniProtKB-KW
    11. post-embryonic development Source: MGI
    12. response to amphetamine Source: MGI
    13. response to cocaine Source: Ensembl
    14. response to corticosterone Source: Ensembl
    15. response to herbicide Source: Ensembl
    16. response to starvation Source: Ensembl
    17. response to toxic substance Source: MGI
    18. response to zinc ion Source: Ensembl

    Keywords - Biological processi

    Neurotransmitter transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Synaptic vesicular amine transporter
    Alternative name(s):
    Monoamine transporter
    Solute carrier family 18 member 2
    Vesicular amine transporter 2
    Short name:
    VAT2
    Gene namesi
    Name:Slc18a2
    Synonyms:Vmat2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:106677. Slc18a2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    2. dense core granule Source: Ensembl
    3. integral component of membrane Source: UniProtKB-KW
    4. neuronal cell body Source: Ensembl
    5. synaptic vesicle membrane Source: Ensembl
    6. terminal bouton Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 517517Synaptic vesicular amine transporterPRO_0000127515Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi91 – 911N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi120 ↔ 327By similarity
    Modified residuei514 – 5141Phosphoserine; by CK2By similarity
    Modified residuei516 – 5161Phosphoserine; by CK2By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ8BRU6.
    PRIDEiQ8BRU6.

    PTM databases

    PhosphoSiteiQ8BRU6.

    Expressioni

    Gene expression databases

    BgeeiQ8BRU6.
    GenevestigatoriQ8BRU6.

    Interactioni

    Subunit structurei

    Interacts with SLC6A3.1 Publication

    Protein-protein interaction databases

    MINTiMINT-577047.
    STRINGi10090.ENSMUSP00000026084.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BRU6.
    SMRiQ8BRU6. Positions 132-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2020CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini42 – 13291Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini154 – 1629CytoplasmicSequence Analysis
    Topological domaini184 – 1929Lumenal, vesicleSequence Analysis
    Topological domaini214 – 2229CytoplasmicSequence Analysis
    Topological domaini246 – 2516Lumenal, vesicleSequence Analysis
    Topological domaini275 – 29420CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini315 – 33117Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini356 – 3605CytoplasmicSequence Analysis
    Topological domaini382 – 39211Lumenal, vesicleSequence AnalysisAdd
    BLAST
    Topological domaini414 – 4174CytoplasmicSequence Analysis
    Topological domaini439 – 4435Lumenal, vesicleSequence Analysis
    Topological domaini466 – 51752CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei21 – 4121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei133 – 15321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei163 – 18321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei193 – 21321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei223 – 24523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei252 – 27423HelicalSequence AnalysisAdd
    BLAST
    Transmembranei295 – 31420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei332 – 35524HelicalSequence AnalysisAdd
    BLAST
    Transmembranei361 – 38121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei393 – 41321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei418 – 43821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei444 – 46522HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG255549.
    GeneTreeiENSGT00390000003851.
    HOGENOMiHOG000018545.
    HOVERGENiHBG055082.
    InParanoidiQ8BRU6.
    KOiK08155.
    OMAiRIGYQIP.
    OrthoDBiEOG79CXZ7.
    PhylomeDBiQ8BRU6.
    TreeFamiTF313494.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004734. Multidrug-R.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BRU6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS    50
    IKHEKNTTEI QTARPALTAS TSESFHSIFS YYNNSTVFTG NATGGLPGGE 100
    SPKATTTQHT VTNTTVPPDC PSEDKDLLNE NVQVGLLFAS KATVQLLTNP 150
    FIGLLTNRIG YPIPMFAGFC IMFISTVMFA FSSSYAFLLI ARSLQGIGSS 200
    CSSVAGMGML ASVYTDDEER GNAMGIALGG LAMGVLVGPP FGSVLYEFVG 250
    KTAPFLVLAA LVLLDGAIQL FVLQPSRVQP ESQKGTPLTT LLKDPYILIA 300
    AGSICFANMG IAMLEPALPI WMMETMCSRK WQLGVAFLPA SISYLIGTNI 350
    FGILAHKMGR WLCALLGMIV VGISILCIPF AKNIYGLIAP NFGVGFAIGM 400
    VDSSMMPIMG YLVDLRHVSV YGSVYAIADV AFCMGYAIGP SAGGAIAKAI 450
    GFPWLMTIIG IIDIVFAPLC FFLRSPPAKE EKMAILMDHN CPIKTKMYTQ 500
    NNVQPYPVGD DEESESD 517
    Length:517
    Mass (Da):55,754
    Last modified:March 1, 2003 - v1
    Checksum:i97B40B65DE3DDBEE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti150 – 1501P → T in BAC28501. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ555564 mRNA. Translation: CAD88262.1.
    AK033871 mRNA. Translation: BAC28501.1.
    AK041274 mRNA. Translation: BAC30887.1.
    CCDSiCCDS29935.1.
    RefSeqiNP_766111.1. NM_172523.3.
    UniGeneiMm.268797.
    Mm.361919.

    Genome annotation databases

    EnsembliENSMUST00000026084; ENSMUSP00000026084; ENSMUSG00000025094.
    GeneIDi214084.
    KEGGimmu:214084.
    UCSCiuc008ibi.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ555564 mRNA. Translation: CAD88262.1 .
    AK033871 mRNA. Translation: BAC28501.1 .
    AK041274 mRNA. Translation: BAC30887.1 .
    CCDSi CCDS29935.1.
    RefSeqi NP_766111.1. NM_172523.3.
    UniGenei Mm.268797.
    Mm.361919.

    3D structure databases

    ProteinModelPortali Q8BRU6.
    SMRi Q8BRU6. Positions 132-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-577047.
    STRINGi 10090.ENSMUSP00000026084.

    PTM databases

    PhosphoSitei Q8BRU6.

    Proteomic databases

    PaxDbi Q8BRU6.
    PRIDEi Q8BRU6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026084 ; ENSMUSP00000026084 ; ENSMUSG00000025094 .
    GeneIDi 214084.
    KEGGi mmu:214084.
    UCSCi uc008ibi.2. mouse.

    Organism-specific databases

    CTDi 6571.
    MGIi MGI:106677. Slc18a2.

    Phylogenomic databases

    eggNOGi NOG255549.
    GeneTreei ENSGT00390000003851.
    HOGENOMi HOG000018545.
    HOVERGENi HBG055082.
    InParanoidi Q8BRU6.
    KOi K08155.
    OMAi RIGYQIP.
    OrthoDBi EOG79CXZ7.
    PhylomeDBi Q8BRU6.
    TreeFami TF313494.

    Miscellaneous databases

    NextBioi 374178.
    PROi Q8BRU6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BRU6.
    Genevestigatori Q8BRU6.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004734. Multidrug-R.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 2 hits.
    TIGRFAMsi TIGR00880. 2_A_01_02. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and pharmacological characterization of the mouse vesicular monoamine transporter 2 (mVMAT2)."
      Gilsbach R., Bonisch H., Bruess M.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Aorta, Diencephalon and Vein.
    3. "Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3."
      Egana L.A., Cuevas R.A., Baust T.B., Parra L.A., Leak R.K., Hochendoner S., Pena K., Quiroz M., Hong W.C., Dorostkar M.M., Janz R., Sitte H.H., Torres G.E.
      J. Neurosci. 29:4592-4604(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SLC6A3.

    Entry informationi

    Entry nameiVMAT2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BRU6
    Secondary accession number(s): Q8CC55
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3