Q8BRU6 (VMAT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptic vesicular amine transporter

Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name=VAT2

Gene names

Name:	Slc18a2
Synonyms:	Vmat2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis By similarity.
Subunit structure	Interacts with SLC6A3. Ref.3
Subcellular location	Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the major facilitator superfamily. Vesicular transporter family.

Ontologies

Keywords
Biological process	Neurotransmitter transport Transport
Cellular component	Cytoplasmic vesicle Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from electronic annotation. Source: Compara cellular response to ammonium ion Inferred from electronic annotation. Source: Compara cellular response to drug Inferred from electronic annotation. Source: Compara death Inferred from mutant phenotype PubMed 9275230. Source: MGI drug transmembrane transport Inferred from electronic annotation. Source: GOC endocytic recycling Inferred from electronic annotation. Source: Compara glucose homeostasis Inferred from electronic annotation. Source: Compara insulin secretion Inferred from electronic annotation. Source: Compara locomotory behavior Inferred from mutant phenotype PubMed 9275230. Source: MGI negative regulation of neurotransmitter transport Inferred from electronic annotation. Source: Compara neurotransmitter transport Inferred from electronic annotation. Source: UniProtKB-KW post-embryonic development Inferred from mutant phenotype PubMed 9275230. Source: MGI response to amphetamine Inferred from mutant phenotype PubMed 9275230. Source: MGI response to cocaine Inferred from electronic annotation. Source: Compara response to corticosterone stimulus Inferred from electronic annotation. Source: Compara response to herbicide Inferred from electronic annotation. Source: Compara response to starvation Inferred from electronic annotation. Source: Compara response to toxin Inferred from mutant phenotype PubMed 9275230. Source: MGI response to zinc ion Inferred from electronic annotation. Source: Compara
Cellular_component	dense core granule Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from electronic annotation. Source: Compara synaptic vesicle membrane Inferred from electronic annotation. Source: Compara terminal bouton Inferred from electronic annotation. Source: Compara
Molecular_function	drug binding Inferred from electronic annotation. Source: Compara drug transmembrane transporter activity Inferred from electronic annotation. Source: InterPro serotonin transmembrane transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Synaptic vesicular amine transporter		PRO_0000127515
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Potential
	Topological domain	42 – 132	91	Lumenal, vesicle Potential
	Transmembrane	133 – 153	21	Helical; Potential
	Topological domain	154 – 162	9	Cytoplasmic Potential
	Transmembrane	163 – 183	21	Helical; Potential
	Topological domain	184 – 192	9	Lumenal, vesicle Potential
	Transmembrane	193 – 213	21	Helical; Potential
	Topological domain	214 – 222	9	Cytoplasmic Potential
	Transmembrane	223 – 245	23	Helical; Potential
	Topological domain	246 – 251	6	Lumenal, vesicle Potential
	Transmembrane	252 – 274	23	Helical; Potential
	Topological domain	275 – 294	20	Cytoplasmic Potential
	Transmembrane	295 – 314	20	Helical; Potential
	Topological domain	315 – 331	17	Lumenal, vesicle Potential
	Transmembrane	332 – 355	24	Helical; Potential
	Topological domain	356 – 360	5	Cytoplasmic Potential
	Transmembrane	361 – 381	21	Helical; Potential
	Topological domain	382 – 392	11	Lumenal, vesicle Potential
	Transmembrane	393 – 413	21	Helical; Potential
	Topological domain	414 – 417	4	Cytoplasmic Potential
	Transmembrane	418 – 438	21	Helical; Potential
	Topological domain	439 – 443	5	Lumenal, vesicle Potential
	Transmembrane	444 – 465	22	Helical; Potential
	Topological domain	466 – 517	52	Cytoplasmic Potential
Amino acid modifications
	Modified residue	514	1	Phosphoserine; by CK2 By similarity
	Modified residue	516	1	Phosphoserine; by CK2 By similarity
	Glycosylation	56	1	N-linked (GlcNAc...) Potential
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	84	1	N-linked (GlcNAc...) Potential
	Glycosylation	91	1	N-linked (GlcNAc...) Potential
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Disulfide bond	120 ↔ 327		By similarity
Experimental info
	Sequence conflict	150	1	P → T in BAC28501. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8BRU6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 97B40B65DE3DDBEE	FASTA	517	55,754
	10 20 30 40 50 60 MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS IKHEKNTTEI 70 80 90 100 110 120 QTARPALTAS TSESFHSIFS YYNNSTVFTG NATGGLPGGE SPKATTTQHT VTNTTVPPDC 130 140 150 160 170 180 PSEDKDLLNE NVQVGLLFAS KATVQLLTNP FIGLLTNRIG YPIPMFAGFC IMFISTVMFA 190 200 210 220 230 240 FSSSYAFLLI ARSLQGIGSS CSSVAGMGML ASVYTDDEER GNAMGIALGG LAMGVLVGPP 250 260 270 280 290 300 FGSVLYEFVG KTAPFLVLAA LVLLDGAIQL FVLQPSRVQP ESQKGTPLTT LLKDPYILIA 310 320 330 340 350 360 AGSICFANMG IAMLEPALPI WMMETMCSRK WQLGVAFLPA SISYLIGTNI FGILAHKMGR 370 380 390 400 410 420 WLCALLGMIV VGISILCIPF AKNIYGLIAP NFGVGFAIGM VDSSMMPIMG YLVDLRHVSV 430 440 450 460 470 480 YGSVYAIADV AFCMGYAIGP SAGGAIAKAI GFPWLMTIIG IIDIVFAPLC FFLRSPPAKE 490 500 510 EKMAILMDHN CPIKTKMYTQ NNVQPYPVGD DEESESD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and pharmacological characterization of the mouse vesicular monoamine transporter 2 (mVMAT2)." Gilsbach R., Bonisch H., Bruess M. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Aorta, Diencephalon and Vein.
[3]	"Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3." Egana L.A., Cuevas R.A., Baust T.B., Parra L.A., Leak R.K., Hochendoner S., Pena K., Quiroz M., Hong W.C., Dorostkar M.M., Janz R., Sitte H.H., Torres G.E. J. Neurosci. 29:4592-4604(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SLC6A3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ555564 mRNA. Translation: CAD88262.1. AK033871 mRNA. Translation: BAC28501.1. AK041274 mRNA. Translation: BAC30887.1.
IPI	IPI00224463.
RefSeq	NP_766111.1. NM_172523.3.
UniGene	Mm.268797. Mm.361919.
3D structure databases
ProteinModelPortal	Q8BRU6.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-577047.
STRING	10090.ENSMUSP00000026084.
PTM databases
PhosphoSite	Q8BRU6.
Proteomic databases
PaxDb	Q8BRU6.
PRIDE	Q8BRU6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026084; ENSMUSP00000026084; ENSMUSG00000025094.
GeneID	214084.
KEGG	mmu:214084.
UCSC	uc008ibi.2. mouse.
Organism-specific databases
CTD	6571.
MGI	MGI:106677. Slc18a2.
Phylogenomic databases
eggNOG	NOG255549.
GeneTree	ENSGT00390000003851.
HOGENOM	HOG000018545.
HOVERGEN	HBG055082.
InParanoid	Q8BRU6.
KO	K08155.
OMA	TNRIGYQ.
OrthoDB	EOG4DR9C5.
Gene expression databases
Bgee	Q8BRU6.
Genevestigator	Q8BRU6.
GermOnline	ENSMUSG00000025094. Mus musculus.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR004734. Multidrug-R. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00880. 2_A_01_02. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	374178.
SOURCE	Search...

Entry information

Entry name

VMAT2_MOUSE

Accession

Primary (citable) accession number: Q8BRU6
Secondary accession number(s): Q8CC55

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	March 1, 2003
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program