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Q8BRU6

- VMAT2_MOUSE

UniProt

Q8BRU6 - VMAT2_MOUSE

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Protein
Synaptic vesicular amine transporter
Gene
Slc18a2, Vmat2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis By similarity.

GO - Molecular functioni

  1. amine transmembrane transporter activity Source: Ensembl
  2. drug binding Source: Ensembl
  3. drug transmembrane transporter activity Source: InterPro
  4. protein binding Source: UniProtKB
  5. serotonin transmembrane transporter activity Source: Ensembl

GO - Biological processi

  1. aging Source: Ensembl
  2. cellular response to ammonium ion Source: Ensembl
  3. cellular response to drug Source: Ensembl
  4. death Source: MGI
  5. endocytic recycling Source: Ensembl
  6. glucose homeostasis Source: Ensembl
  7. insulin secretion Source: Ensembl
  8. locomotory behavior Source: MGI
  9. negative regulation of neurotransmitter transport Source: Ensembl
  10. neurotransmitter transport Source: UniProtKB-KW
  11. post-embryonic development Source: MGI
  12. response to amphetamine Source: MGI
  13. response to cocaine Source: Ensembl
  14. response to corticosterone Source: Ensembl
  15. response to herbicide Source: Ensembl
  16. response to starvation Source: Ensembl
  17. response to toxic substance Source: MGI
  18. response to zinc ion Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptic vesicular amine transporter
Alternative name(s):
Monoamine transporter
Solute carrier family 18 member 2
Vesicular amine transporter 2
Short name:
VAT2
Gene namesi
Name:Slc18a2
Synonyms:Vmat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:106677. Slc18a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei21 – 4121Helical; Reviewed prediction
Add
BLAST
Topological domaini42 – 13291Lumenal, vesicle Reviewed prediction
Add
BLAST
Transmembranei133 – 15321Helical; Reviewed prediction
Add
BLAST
Topological domaini154 – 1629Cytoplasmic Reviewed prediction
Transmembranei163 – 18321Helical; Reviewed prediction
Add
BLAST
Topological domaini184 – 1929Lumenal, vesicle Reviewed prediction
Transmembranei193 – 21321Helical; Reviewed prediction
Add
BLAST
Topological domaini214 – 2229Cytoplasmic Reviewed prediction
Transmembranei223 – 24523Helical; Reviewed prediction
Add
BLAST
Topological domaini246 – 2516Lumenal, vesicle Reviewed prediction
Transmembranei252 – 27423Helical; Reviewed prediction
Add
BLAST
Topological domaini275 – 29420Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei295 – 31420Helical; Reviewed prediction
Add
BLAST
Topological domaini315 – 33117Lumenal, vesicle Reviewed prediction
Add
BLAST
Transmembranei332 – 35524Helical; Reviewed prediction
Add
BLAST
Topological domaini356 – 3605Cytoplasmic Reviewed prediction
Transmembranei361 – 38121Helical; Reviewed prediction
Add
BLAST
Topological domaini382 – 39211Lumenal, vesicle Reviewed prediction
Add
BLAST
Transmembranei393 – 41321Helical; Reviewed prediction
Add
BLAST
Topological domaini414 – 4174Cytoplasmic Reviewed prediction
Transmembranei418 – 43821Helical; Reviewed prediction
Add
BLAST
Topological domaini439 – 4435Lumenal, vesicle Reviewed prediction
Transmembranei444 – 46522Helical; Reviewed prediction
Add
BLAST
Topological domaini466 – 51752Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. dense core granule Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
  3. neuronal cell body Source: Ensembl
  4. synaptic vesicle membrane Source: Ensembl
  5. terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Synaptic vesicular amine transporter
PRO_0000127515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi83 – 831N-linked (GlcNAc...) Reviewed prediction
Glycosylationi84 – 841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi91 – 911N-linked (GlcNAc...) Reviewed prediction
Glycosylationi113 – 1131N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi120 ↔ 327 By similarity
Modified residuei514 – 5141Phosphoserine; by CK2 By similarity
Modified residuei516 – 5161Phosphoserine; by CK2 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ8BRU6.
PRIDEiQ8BRU6.

PTM databases

PhosphoSiteiQ8BRU6.

Expressioni

Gene expression databases

BgeeiQ8BRU6.
GenevestigatoriQ8BRU6.

Interactioni

Subunit structurei

Interacts with SLC6A3.1 Publication

Protein-protein interaction databases

MINTiMINT-577047.
STRINGi10090.ENSMUSP00000026084.

Structurei

3D structure databases

ProteinModelPortaliQ8BRU6.
SMRiQ8BRU6. Positions 132-446.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG255549.
GeneTreeiENSGT00390000003851.
HOGENOMiHOG000018545.
HOVERGENiHBG055082.
InParanoidiQ8BRU6.
KOiK08155.
OMAiRIGYQIP.
OrthoDBiEOG79CXZ7.
PhylomeDBiQ8BRU6.
TreeFamiTF313494.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00880. 2_A_01_02. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BRU6-1 [UniParc]FASTAAdd to Basket

« Hide

MALSDLVLLR WLRDSRHSRK LILFIVFLAL LLDNMLLTVV VPIIPSYLYS    50
IKHEKNTTEI QTARPALTAS TSESFHSIFS YYNNSTVFTG NATGGLPGGE 100
SPKATTTQHT VTNTTVPPDC PSEDKDLLNE NVQVGLLFAS KATVQLLTNP 150
FIGLLTNRIG YPIPMFAGFC IMFISTVMFA FSSSYAFLLI ARSLQGIGSS 200
CSSVAGMGML ASVYTDDEER GNAMGIALGG LAMGVLVGPP FGSVLYEFVG 250
KTAPFLVLAA LVLLDGAIQL FVLQPSRVQP ESQKGTPLTT LLKDPYILIA 300
AGSICFANMG IAMLEPALPI WMMETMCSRK WQLGVAFLPA SISYLIGTNI 350
FGILAHKMGR WLCALLGMIV VGISILCIPF AKNIYGLIAP NFGVGFAIGM 400
VDSSMMPIMG YLVDLRHVSV YGSVYAIADV AFCMGYAIGP SAGGAIAKAI 450
GFPWLMTIIG IIDIVFAPLC FFLRSPPAKE EKMAILMDHN CPIKTKMYTQ 500
NNVQPYPVGD DEESESD 517
Length:517
Mass (Da):55,754
Last modified:March 1, 2003 - v1
Checksum:i97B40B65DE3DDBEE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti150 – 1501P → T in BAC28501. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ555564 mRNA. Translation: CAD88262.1.
AK033871 mRNA. Translation: BAC28501.1.
AK041274 mRNA. Translation: BAC30887.1.
CCDSiCCDS29935.1.
RefSeqiNP_766111.1. NM_172523.3.
UniGeneiMm.268797.
Mm.361919.

Genome annotation databases

EnsembliENSMUST00000026084; ENSMUSP00000026084; ENSMUSG00000025094.
GeneIDi214084.
KEGGimmu:214084.
UCSCiuc008ibi.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ555564 mRNA. Translation: CAD88262.1 .
AK033871 mRNA. Translation: BAC28501.1 .
AK041274 mRNA. Translation: BAC30887.1 .
CCDSi CCDS29935.1.
RefSeqi NP_766111.1. NM_172523.3.
UniGenei Mm.268797.
Mm.361919.

3D structure databases

ProteinModelPortali Q8BRU6.
SMRi Q8BRU6. Positions 132-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-577047.
STRINGi 10090.ENSMUSP00000026084.

PTM databases

PhosphoSitei Q8BRU6.

Proteomic databases

PaxDbi Q8BRU6.
PRIDEi Q8BRU6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000026084 ; ENSMUSP00000026084 ; ENSMUSG00000025094 .
GeneIDi 214084.
KEGGi mmu:214084.
UCSCi uc008ibi.2. mouse.

Organism-specific databases

CTDi 6571.
MGIi MGI:106677. Slc18a2.

Phylogenomic databases

eggNOGi NOG255549.
GeneTreei ENSGT00390000003851.
HOGENOMi HOG000018545.
HOVERGENi HBG055082.
InParanoidi Q8BRU6.
KOi K08155.
OMAi RIGYQIP.
OrthoDBi EOG79CXZ7.
PhylomeDBi Q8BRU6.
TreeFami TF313494.

Miscellaneous databases

NextBioi 374178.
PROi Q8BRU6.
SOURCEi Search...

Gene expression databases

Bgeei Q8BRU6.
Genevestigatori Q8BRU6.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004734. Multidrug-R.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 2 hits.
TIGRFAMsi TIGR00880. 2_A_01_02. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and pharmacological characterization of the mouse vesicular monoamine transporter 2 (mVMAT2)."
    Gilsbach R., Bonisch H., Bruess M.
    Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Aorta, Diencephalon and Vein.
  3. "Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3."
    Egana L.A., Cuevas R.A., Baust T.B., Parra L.A., Leak R.K., Hochendoner S., Pena K., Quiroz M., Hong W.C., Dorostkar M.M., Janz R., Sitte H.H., Torres G.E.
    J. Neurosci. 29:4592-4604(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLC6A3.

Entry informationi

Entry nameiVMAT2_MOUSE
AccessioniPrimary (citable) accession number: Q8BRU6
Secondary accession number(s): Q8CC55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi