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Protein

CLIP-associating protein 2

Gene

Clasp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.By similarity1 Publication

GO - Molecular functioni

  • dystroglycan binding Source: MGI
  • microtubule binding Source: MGI
  • microtubule plus-end binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
CLIP-associating protein 2
Alternative name(s):
Cytoplasmic linker-associated protein 2
Gene namesi
Name:Clasp2
Synonyms:Kiaa0627
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1923749. Clasp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Centromere, Chromosome, Cytoplasm, Cytoskeleton, Golgi apparatus, Kinetochore, Membrane, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6C → S: Impairs Golgi localization; when associated with S-10. 1 Publication1
Mutagenesisi10C → S: Impairs Golgi localization; when associated with S-6. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000898501 – 1286CLIP-associating protein 2Add BLAST1286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei14PhosphoserineCombined sources1
Modified residuei20PhosphoserineBy similarity1
Modified residuei322PhosphoserineBy similarity1
Modified residuei333PhosphoserineBy similarity1
Modified residuei336PhosphoserineBy similarity1
Modified residuei374PhosphoserineCombined sources1
Modified residuei376PhosphoserineCombined sources1
Modified residuei413PhosphoserineBy similarity1
Modified residuei461PhosphoserineBy similarity1
Modified residuei465PhosphoserineBy similarity1
Modified residuei469PhosphoserineBy similarity1
Modified residuei484PhosphoserineBy similarity1
Modified residuei495PhosphoserineBy similarity1
Modified residuei513PhosphoserineBy similarity1
Modified residuei531PhosphoserineBy similarity1
Modified residuei535PhosphoserineBy similarity1
Modified residuei570PhosphoserineBy similarity1
Modified residuei572PhosphoserineBy similarity1
Modified residuei581PhosphoserineBy similarity1
Modified residuei614PhosphoserineBy similarity1
Modified residuei620PhosphoserineCombined sources1
Modified residuei779PhosphothreonineBy similarity1
Modified residuei884PhosphoserineBy similarity1
Modified residuei944PhosphoserineBy similarity1
Modified residuei947PhosphoserineCombined sources1
Modified residuei1005PhosphoserineBy similarity1
Modified residuei1021PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by GSK3B. Phosphorylation by GSK3B may negatively regulate binding to microtubule lattices in lamella. Isoform 2 is phosphorylated on Ser-241.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BRT1.
PaxDbiQ8BRT1.
PeptideAtlasiQ8BRT1.
PRIDEiQ8BRT1.

PTM databases

iPTMnetiQ8BRT1.
PhosphoSitePlusiQ8BRT1.

Expressioni

Tissue specificityi

Highly expressed in brain and at low levels in heart, kidney and lung.1 Publication

Developmental stagei

Expressed in oocytes and early embryos.1 Publication

Gene expression databases

CleanExiMM_CLASP2.

Interactioni

Subunit structurei

Interacts with microtubules (PubMed:11290329). Interacts with MAPRE1; probably required for targeting to growing microtubule plus ends. Interacts with ERC1, MAPRE3 and PHLDB2. The interaction with ERC1 may be mediated by PHLDB2. Interacts with GCC2; recruits CLASP2 to Golgi membranes (By similarity). Interacts with CLIP2 and RSN (PubMed:11290329). Interacts with MACF1 (PubMed:18854161).By similarity2 Publications

GO - Molecular functioni

  • dystroglycan binding Source: MGI
  • microtubule binding Source: MGI
  • microtubule plus-end binding Source: UniProtKB

Protein-protein interaction databases

BioGridi218155. 7 interactors.
IntActiQ8BRT1. 5 interactors.
MINTiMINT-4112101.
STRINGi10090.ENSMUSP00000130201.

Structurei

Secondary structure

11286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi648 – 655Combined sources8
Helixi660 – 675Combined sources16
Helixi682 – 695Combined sources14
Helixi701 – 718Combined sources18
Helixi719 – 721Combined sources3
Turni723 – 725Combined sources3
Helixi726 – 737Combined sources12
Helixi743 – 759Combined sources17
Helixi762 – 774Combined sources13
Beta strandi776 – 778Combined sources3
Helixi782 – 796Combined sources15
Helixi801 – 803Combined sources3
Helixi808 – 820Combined sources13
Helixi827 – 843Combined sources17
Helixi845 – 853Combined sources9
Helixi857 – 867Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WOZX-ray2.20A/B/C/D642-873[»]
ProteinModelPortaliQ8BRT1.
SMRiQ8BRT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati179 – 214HEAT 1Sequence analysisAdd BLAST36
Repeati215 – 251HEAT 2Sequence analysisAdd BLAST37
Repeati256 – 293HEAT 3Sequence analysisAdd BLAST38
Repeati702 – 739HEAT 4Sequence analysisAdd BLAST38
Repeati764 – 801HEAT 5Sequence analysisAdd BLAST38
Repeati1046 – 1083HEAT 6Sequence analysisAdd BLAST38
Repeati1090 – 1127HEAT 7Sequence analysisAdd BLAST38
Repeati1208 – 1245HEAT 8Sequence analysisAdd BLAST38

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 40Golgi localizationAdd BLAST40
Regioni66 – 317TOG 1By similarityAdd BLAST252
Regioni450 – 565Interaction with microtubules, MAPRE1 and MAPRE3By similarityAdd BLAST116
Regioni642 – 873TOG 2By similarityAdd BLAST232
Regioni864 – 1286Interaction with RSN and localization to the Golgi and kinetochoresBy similarityAdd BLAST423
Regioni1009 – 1286Required for cortical localizationBy similarityAdd BLAST278

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi500 – 503SXIP motif 1; mediates interaction with MAPRE1 and targeting to microtubule plus endsBy similarity4
Motifi523 – 526SXIP motif 2; mediates interaction with MAPRE1 and targeting to microtubule plus endsBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi459 – 572Ser-richAdd BLAST114

Domaini

The two SXIP sequence motifs mediate interaction with MAPRE1; this is necessary for targeting to growing microtubule plus ends.By similarity
Two TOG regions display structural characteristics similar to HEAT repeat domains and mediate interaction with microtubules.By similarity1 Publication

Sequence similaritiesi

Belongs to the CLASP family.Curated
Contains 8 HEAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2956. Eukaryota.
ENOG410ZMY0. LUCA.
HOGENOMiHOG000185856.
HOVERGENiHBG079692.
InParanoidiQ8BRT1.
KOiK16578.
PhylomeDBiQ8BRT1.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024395. CLASP_N_dom.
[Graphical view]
PfamiPF12348. CLASP_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BRT1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRLICKRIC DYKSFDDEES VDGNRPSSAA SAFKVPAPKT PGNPVSSARK
60 70 80 90 100
PGSAGGPKVG GPSKEGGAGA VDEDDFIKAF TDVPSVQIYS SRELEETLNK
110 120 130 140 150
IREILSDDKH DWDQRANALK KIRSLLVAGA AQYDCFFQHL RLLDGALKLS
160 170 180 190 200
AKDLRSQVVR EACITVAHLS TVLGNKFDHG AEAIVPTLFN LVPNSAKVMA
210 220 230 240 250
TSGCAAIRFI IRHTHVPRLI PLITSNCTSK SVPVRRRSFE FLDLLLQEWQ
260 270 280 290 300
THSLERHAAV LVETIKKGIH DADAEARVEA RKTYMGLRNH FPGEAETLYN
310 320 330 340 350
SLEPSYQKSL QTYLKSSGSV ASLPQSDRSS SSSQESLNRP FSSKWSTANP
360 370 380 390 400
STVAGRVSVG GSKANPLPGS LQRSRSDIDV NAAAGAKAHH AAGQAVRSGR
410 420 430 440 450
LGAGALNPGS YASLEDTSDK MDGTASDDGR VRAKLSTPLV AVGNAKTDSR
460 470 480 490 500
GRSRTKMVSQ SQPGSRSGSP GRVLTTTALS TVSSGAQRVL VNSASAQKRS
510 520 530 540 550
KIPRSQGCSR EASPSRLSVA RSSRIPRPSV SQGCSREASR ESSRDTSPVR
560 570 580 590 600
SFQPLGPGYG ISQSSRLSSS VSAMRVLNTG SDVEEAVADA LLLGDIRTKK
610 620 630 640 650
KPARRRYESY GMHSDDDANS DASSACSERS YSSRNGSIPT YMRQTEDVAE
660 670 680 690 700
VLNRCASSNW SERKEGLLGL QNLLKNQRTL SRIELKRLCE IFTRMFADPH
710 720 730 740 750
GKVFSMFLET LVDFIQVHKD DLQDWLFVLL TQLLKKMGAD LLGSVQAKVQ
760 770 780 790 800
KALDITRESF PNDLQFNILM RFTVDQTQTP SLKVKVAILK YIETLAKQMD
810 820 830 840 850
PRDFTNSSET RLAVSRVITW TTEPKSSDVR KAAQSVLISL FELNTPEFTM
860 870 880 890 900
LLGALPKTFQ DGATKLLHNH LRNTGNGTQS SMGSPLTRPT PRSPANWSSP
910 920 930 940 950
LTSPTNTSQN TLSPSAFDYD TENMNSEDIY SSLRGVTEAI QNFSFRSQED
960 970 980 990 1000
MSEPVRRDPK KEDGDTICSG PGMSDPRAGG DAADGSQPAL DNKASLLHSM
1010 1020 1030 1040 1050
PLHSSPRSRD YNPYNYSDSI SPFNKSALKE AMFDDDADQF PDDLSLDHSD
1060 1070 1080 1090 1100
LVAELLKELS NHNERIEERK IALYELMKLT QEESFSVWDE HFKTILLLLL
1110 1120 1130 1140 1150
ETLGDKEPTI RALALKVLKE ILRHQPARFK NYAELTVMKT LEAHKDPHKE
1160 1170 1180 1190 1200
VVRSAEEAAS VLATSISPEQ CIKVLCPIIQ TADYPINLAA IKMQTKVIER
1210 1220 1230 1240 1250
VSKETLNMLL PEIMPGLIQG YDNSESSVRK ACVFCLVAVH AVIGDELKPH
1260 1270 1280
LSQLTGSKMK LLNLYIKRAQ TGSAGADPTA DVSGQS
Length:1,286
Mass (Da):140,739
Last modified:March 1, 2003 - v1
Checksum:i5B208D8F4E90CB93
GO
Isoform 2 (identifier: Q8BRT1-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MRRLICKRICDY → MEPRGAEYFC...GGMILSVCKD
     142-1054: Missing.

Show »
Length:600
Mass (Da):66,370
Checksum:iF7ADD06A38B893D3
GO
Isoform 3 (identifier: Q8BRT1-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-12: MRRLICKRICDY → MAMGDD
     119-173: LKKIRSLLVA...ITVAHLSTVL → CMSCSLVASE...SPVSSAFVQH
     174-1286: Missing.

Show »
Length:167
Mass (Da):18,021
Checksum:i5512515D37A0CF08
GO

Sequence cautioni

The sequence BAC41436 differs from that shown. Reason: Frameshift at position 737.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti657S → T in BAC41436 (PubMed:12465718).Curated1
Sequence conflicti683I → V in BAC41436 (PubMed:12465718).Curated1
Sequence conflicti699P → L in BAC41436 (PubMed:12465718).Curated1
Sequence conflicti702K → KR in BAC41436 (PubMed:12465718).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0158081 – 12MRRLI…RICDY → MEPRGAEYFCAQVLQKDVSG RLQAGEELLLCLGTPGAIPD LEDDPSRLAKTVDALTRWVG SSNYRVSLLGLEILSAFVDR LSTRFKSYVTMVTTALIDRM GDVKDKVREEAQNLTLKLMD EVAPPMYIWEQLASGFKHKN FRSREGVCLCLIETLNIFGT QPLVISKLVPHLCVLFGDSN SQVRNAALSAVVEIYRHVGE KLRIDLCKRDIPPARLEMVL AKFDEVQNSGGMILSVCKD in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_0158091 – 12MRRLI…RICDY → MAMGDD in isoform 3. 2 PublicationsAdd BLAST12
Alternative sequenceiVSP_015810119 – 173LKKIR…LSTVL → CMSCSLVASEVERALAWPLW SHLATYQHHCHCTLSHEDLP EKRLTSPVSSAFVQH in isoform 3. 2 PublicationsAdd BLAST55
Alternative sequenceiVSP_015811142 – 1054Missing in isoform 2. 1 PublicationAdd BLAST913
Alternative sequenceiVSP_015812174 – 1286Missing in isoform 3. 2 PublicationsAdd BLAST1113

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276961 mRNA. Translation: CAC35161.1.
AK043551 mRNA. Translation: BAC31579.1.
AK005146 mRNA. Translation: BAB23842.1.
BC030468 mRNA. Translation: AAH30468.1.
AB093252 Transcribed RNA. Translation: BAC41436.2. Frameshift.
CCDSiCCDS52948.1. [Q8BRT1-1]
RefSeqiNP_001273528.1. NM_001286599.1.
NP_001273529.1. NM_001286600.1.
NP_001273530.1. NM_001286601.1.
NP_001273531.1. NM_001286602.1.
NP_001273532.1. NM_001286603.1. [Q8BRT1-6]
UniGeneiMm.222272.

Genome annotation databases

GeneIDi76499.
KEGGimmu:76499.
UCSCiuc009rwt.2. mouse. [Q8BRT1-6]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276961 mRNA. Translation: CAC35161.1.
AK043551 mRNA. Translation: BAC31579.1.
AK005146 mRNA. Translation: BAB23842.1.
BC030468 mRNA. Translation: AAH30468.1.
AB093252 Transcribed RNA. Translation: BAC41436.2. Frameshift.
CCDSiCCDS52948.1. [Q8BRT1-1]
RefSeqiNP_001273528.1. NM_001286599.1.
NP_001273529.1. NM_001286600.1.
NP_001273530.1. NM_001286601.1.
NP_001273531.1. NM_001286602.1.
NP_001273532.1. NM_001286603.1. [Q8BRT1-6]
UniGeneiMm.222272.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WOZX-ray2.20A/B/C/D642-873[»]
ProteinModelPortaliQ8BRT1.
SMRiQ8BRT1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218155. 7 interactors.
IntActiQ8BRT1. 5 interactors.
MINTiMINT-4112101.
STRINGi10090.ENSMUSP00000130201.

PTM databases

iPTMnetiQ8BRT1.
PhosphoSitePlusiQ8BRT1.

Proteomic databases

EPDiQ8BRT1.
PaxDbiQ8BRT1.
PeptideAtlasiQ8BRT1.
PRIDEiQ8BRT1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi76499.
KEGGimmu:76499.
UCSCiuc009rwt.2. mouse. [Q8BRT1-6]

Organism-specific databases

CTDi23122.
MGIiMGI:1923749. Clasp2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG2956. Eukaryota.
ENOG410ZMY0. LUCA.
HOGENOMiHOG000185856.
HOVERGENiHBG079692.
InParanoidiQ8BRT1.
KOiK16578.
PhylomeDBiQ8BRT1.

Miscellaneous databases

PROiQ8BRT1.
SOURCEiSearch...

Gene expression databases

CleanExiMM_CLASP2.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024395. CLASP_N_dom.
[Graphical view]
PfamiPF12348. CLASP_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 4 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCLAP2_MOUSE
AccessioniPrimary (citable) accession number: Q8BRT1
Secondary accession number(s): Q8CHE3, Q99JI3, Q9DB80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.