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Protein

Alpha-mannosidase 2x

Gene

Man2a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway (By similarity).By similarity

Catalytic activityi

Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi175 – 1751ZincBy similarity
Metal bindingi177 – 1771ZincBy similarity
Active sitei289 – 2891NucleophileBy similarity
Metal bindingi289 – 2891ZincBy similarity
Metal bindingi569 – 5691ZincBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. hydrolase activity, hydrolyzing N-glycosyl compounds Source: MGI
  3. mannosidase activity Source: MGI
  4. mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Source: UniProtKB-EC
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. mannose metabolic process Source: InterPro
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_278515. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase 2x (EC:3.2.1.114)
Alternative name(s):
Alpha-mannosidase IIx
Short name:
Man IIx
Mannosidase alpha class 2A member 2
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene namesi
Name:Man2a2
Synonyms:Mana2x
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2150656. Man2a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55CytoplasmicSequence Analysis
Transmembranei6 – 2621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini27 – 796770LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11521152Alpha-mannosidase 2xPRO_0000412634Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi225 – 2251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8BRK9.
PRIDEiQ8BRK9.

Expressioni

Gene expression databases

BgeeiQ8BRK9.
ExpressionAtlasiQ8BRK9. baseline and differential.
GenevestigatoriQ8BRK9.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095949.

Structurei

3D structure databases

ProteinModelPortaliQ8BRK9.
SMRiQ8BRK9. Positions 762-1014.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili43 – 7432Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 38 family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046304.
HOVERGENiHBG052390.
InParanoidiQ8BRK9.
KOiK01231.
OMAiFNPSEQT.
OrthoDBiEOG7JQBMJ.
PhylomeDBiQ8BRK9.
TreeFamiTF313152.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BRK9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLKKQVTVC GAAIFCVAVF SLYLMLDRVQ HDPARHQNGG NFPRSQISVL
60 70 80 90 100
QNRIEQLEQL LEENHDIISR IKDSVLELTA NAEGPPALLP YHTANGSWAV
110 120 130 140 150
LPEPRPSFFS VSPQDCQFAL GGRGQKPELQ MLTVSEDLPF DNVEGGVWRQ
160 170 180 190 200
GFDISYSPND WDTEDLQVFV VPHSHNDPGW IKTFDKYYTE QTQHILNSMV
210 220 230 240 250
SKLQEDPRRR FLWAEVSFFA KWWDNISAQK RAAVRRLVGN GQLEIATGGW
260 270 280 290 300
VMPDEANSHY FALVDQLIEG HQWLERNLGA TPRSGWAVDP FGHSSTMPYL
310 320 330 340 350
LRRANLTSML IQRVHYAIKK HFAATHSLEF MWRQMWDSDS STDIFCHMMP
360 370 380 390 400
FYSYDVPHTC GPDPKICCQF DFKRLPGGRI NCPWKVPPRA ITEANVADRA
410 420 430 440 450
ALLLDQYRKK SRLFRSNVLL VPLGDDFRYD KPQEWDAQFF NYQRLFDFLN
460 470 480 490 500
SKPEFHVQAQ FGTLSEYFDA LYKRTGVEPG ARPPGFPVLS GDFFSYADRE
510 520 530 540 550
DHYWTGYYTS RPFYKSLDRV LEAHLRGAEI LYSLALAHAR RSGLAGQYPL
560 570 580 590 600
SDFALLTEAR RTLGLFQHHD AITGTAKEAV VVDYGVRLLR SLVSLKQVII
610 620 630 640 650
NAAHYLVLGD QETYSFDPGT PFLQMDDSRV SHDALPERTV IRLDSSPRFV
660 670 680 690 700
VVFNPLEQER LSVVSLLVNS PRVRVLSEEG QPLSVQISVH WSSATDMVPD
710 720 730 740 750
VYQVSVPVRL PGLGLGVLQL QPDLDGPYTL QSSVRVYLNG VKLSVSRQSA
760 770 780 790 800
FPVRVVDSGA SDFAISNRYM QVWFSGLTGL LKSIRRVDEE QEQQMELEFL
810 820 830 840 850
VYGTRTSKDK SGAYLFLPDS EAKPYVPKKP PVLRVTEGPF FSEVAVYYEH
860 870 880 890 900
FHQVIRLYNL PGVEGLSLDM SFQVDIRDYV NKELALRIHT DIDSQGTFFT
910 920 930 940 950
DLNGFQIQPR QYLKKLPLQA NFYPMPVMAY IQDSQRRLTL HTAQALGVSS
960 970 980 990 1000
LGNGQLEVIL DRRLMQDDNR GLGQGLKDNK ITCNRFRLLL ERRTTMSPEV
1010 1020 1030 1040 1050
HQEQERSTSY PSLLSHLTSM YLSTPPLVLP VAKRQGTSPA LRSFHPLASP
1060 1070 1080 1090 1100
LPCDFHLLNL RMLPAEDTLP ATDSALILHR KGFDCGLEAK NLGFNCTTSQ
1110 1120 1130 1140 1150
GKLALGSLFH GLDVTFLQPT SLTLLYPLAS PSNSTDISLE PMEISTFRLR

LG
Length:1,152
Mass (Da):130,649
Last modified:September 21, 2011 - v2
Checksum:i8A6274E9671F92DF
GO
Isoform 2 (identifier: Q8BRK9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     783-827: SIRRVDEEQE...PDSEAKPYVP → VKGQARGGGE...PDSQRFGCEH
     828-1152: Missing.

Show »
Length:827
Mass (Da):93,450
Checksum:i31A7AC9C5FC4CF2C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 971S → T in AK029913 (PubMed:16141072).Curated
Sequence conflicti1111 – 11111G → S in BAE23268 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei783 – 82745SIRRV…KPYVP → VKGQARGGGEGPEEGMGVRG LVDSCSCSHVPSCLSPDSQR FGCEH in isoform 2. 1 PublicationVSP_041735Add
BLAST
Alternative sequencei828 – 1152325Missing in isoform 2. 1 PublicationVSP_041736Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029913 mRNA. No translation available.
AK044028 mRNA. Translation: BAC31745.1.
AK137205 mRNA. Translation: BAE23268.1.
AC136740 Genomic DNA. No translation available.
CCDSiCCDS57556.1. [Q8BRK9-1]
RefSeqiNP_766491.2. NM_172903.4. [Q8BRK9-1]
XP_006540661.1. XM_006540598.2. [Q8BRK9-1]
XP_006540662.1. XM_006540599.2. [Q8BRK9-1]
UniGeneiMm.269245.

Genome annotation databases

EnsembliENSMUST00000098346; ENSMUSP00000095949; ENSMUSG00000038886. [Q8BRK9-1]
GeneIDi140481.
KEGGimmu:140481.
UCSCiuc009iap.2. mouse. [Q8BRK9-1]
uc009iar.2. mouse. [Q8BRK9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029913 mRNA. No translation available.
AK044028 mRNA. Translation: BAC31745.1.
AK137205 mRNA. Translation: BAE23268.1.
AC136740 Genomic DNA. No translation available.
CCDSiCCDS57556.1. [Q8BRK9-1]
RefSeqiNP_766491.2. NM_172903.4. [Q8BRK9-1]
XP_006540661.1. XM_006540598.2. [Q8BRK9-1]
XP_006540662.1. XM_006540599.2. [Q8BRK9-1]
UniGeneiMm.269245.

3D structure databases

ProteinModelPortaliQ8BRK9.
SMRiQ8BRK9. Positions 762-1014.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095949.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Proteomic databases

MaxQBiQ8BRK9.
PRIDEiQ8BRK9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000098346; ENSMUSP00000095949; ENSMUSG00000038886. [Q8BRK9-1]
GeneIDi140481.
KEGGimmu:140481.
UCSCiuc009iap.2. mouse. [Q8BRK9-1]
uc009iar.2. mouse. [Q8BRK9-2]

Organism-specific databases

CTDi4122.
MGIiMGI:2150656. Man2a2.

Phylogenomic databases

GeneTreeiENSGT00510000046304.
HOVERGENiHBG052390.
InParanoidiQ8BRK9.
KOiK01231.
OMAiFNPSEQT.
OrthoDBiEOG7JQBMJ.
PhylomeDBiQ8BRK9.
TreeFamiTF313152.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_278515. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

ChiTaRSiMan2a2. mouse.
NextBioi369778.
PROiQ8BRK9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BRK9.
ExpressionAtlasiQ8BRK9. baseline and differential.
GenevestigatoriQ8BRK9.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain cortex, Testis and Urinary bladder.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiMA2A2_MOUSE
AccessioniPrimary (citable) accession number: Q8BRK9
Secondary accession number(s): Q3UVK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: April 1, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.