Q8BRK9 (MA2A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-mannosidase 2x
EC=3.2.1.114

Alternative name(s):
Alpha-mannosidase IIx
Short name=Man IIx
Mannosidase alpha class 2A member 2
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase

Gene names

Name:	Man2a2
Synonyms:	Mana2x

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1152 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway By similarity.
Catalytic activity	Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 38 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Signal-anchor Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mannose metabolic process Inferred from electronic annotation. Source: InterPro protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing N-glycosyl compounds Inferred from direct assay PubMed 16754854. Source: MGI mannosidase activity Inferred from direct assay PubMed 16754854. Source: MGI mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BRK9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BRK9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     783-827: SIRRVDEEQE...PDSEAKPYVP → VKGQARGGGE...PDSQRFGCEH
     828-1152: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1152	1152	Alpha-mannosidase 2x		PRO_0000412634
Regions
	Topological domain	1 – 5	5	Cytoplasmic Potential
	Transmembrane	6 – 26	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	27 – 796	770	Lumenal Potential
	Coiled coil	43 – 74	32	Potential
Sites
	Active site	289	1	Nucleophile By similarity
	Metal binding	175	1	Zinc By similarity
	Metal binding	177	1	Zinc By similarity
	Metal binding	289	1	Zinc By similarity
	Metal binding	569	1	Zinc By similarity
Amino acid modifications
	Glycosylation	225	1	N-linked (GlcNAc...) Potential
	Glycosylation	305	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	783 – 827	45	SIRRV…KPYVP → VKGQARGGGEGPEEGMGVRG LVDSCSCSHVPSCLSPDSQR FGCEH in isoform 2.		VSP_041735
	Alternative sequence	828 – 1152	325	Missing in isoform 2.		VSP_041736
Experimental info
	Sequence conflict	97	1	S → T in AK029913. Ref.1
	Sequence conflict	1111	1	G → S in BAE23268. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 21, 2011. Version 2. Checksum: 8A6274E9671F92DF	FASTA	1,152	130,649
	10 20 30 40 50 60 MKLKKQVTVC GAAIFCVAVF SLYLMLDRVQ HDPARHQNGG NFPRSQISVL QNRIEQLEQL 70 80 90 100 110 120 LEENHDIISR IKDSVLELTA NAEGPPALLP YHTANGSWAV LPEPRPSFFS VSPQDCQFAL 130 140 150 160 170 180 GGRGQKPELQ MLTVSEDLPF DNVEGGVWRQ GFDISYSPND WDTEDLQVFV VPHSHNDPGW 190 200 210 220 230 240 IKTFDKYYTE QTQHILNSMV SKLQEDPRRR FLWAEVSFFA KWWDNISAQK RAAVRRLVGN 250 260 270 280 290 300 GQLEIATGGW VMPDEANSHY FALVDQLIEG HQWLERNLGA TPRSGWAVDP FGHSSTMPYL 310 320 330 340 350 360 LRRANLTSML IQRVHYAIKK HFAATHSLEF MWRQMWDSDS STDIFCHMMP FYSYDVPHTC 370 380 390 400 410 420 GPDPKICCQF DFKRLPGGRI NCPWKVPPRA ITEANVADRA ALLLDQYRKK SRLFRSNVLL 430 440 450 460 470 480 VPLGDDFRYD KPQEWDAQFF NYQRLFDFLN SKPEFHVQAQ FGTLSEYFDA LYKRTGVEPG 490 500 510 520 530 540 ARPPGFPVLS GDFFSYADRE DHYWTGYYTS RPFYKSLDRV LEAHLRGAEI LYSLALAHAR 550 560 570 580 590 600 RSGLAGQYPL SDFALLTEAR RTLGLFQHHD AITGTAKEAV VVDYGVRLLR SLVSLKQVII 610 620 630 640 650 660 NAAHYLVLGD QETYSFDPGT PFLQMDDSRV SHDALPERTV IRLDSSPRFV VVFNPLEQER 670 680 690 700 710 720 LSVVSLLVNS PRVRVLSEEG QPLSVQISVH WSSATDMVPD VYQVSVPVRL PGLGLGVLQL 730 740 750 760 770 780 QPDLDGPYTL QSSVRVYLNG VKLSVSRQSA FPVRVVDSGA SDFAISNRYM QVWFSGLTGL 790 800 810 820 830 840 LKSIRRVDEE QEQQMELEFL VYGTRTSKDK SGAYLFLPDS EAKPYVPKKP PVLRVTEGPF 850 860 870 880 890 900 FSEVAVYYEH FHQVIRLYNL PGVEGLSLDM SFQVDIRDYV NKELALRIHT DIDSQGTFFT 910 920 930 940 950 960 DLNGFQIQPR QYLKKLPLQA NFYPMPVMAY IQDSQRRLTL HTAQALGVSS LGNGQLEVIL 970 980 990 1000 1010 1020 DRRLMQDDNR GLGQGLKDNK ITCNRFRLLL ERRTTMSPEV HQEQERSTSY PSLLSHLTSM 1030 1040 1050 1060 1070 1080 YLSTPPLVLP VAKRQGTSPA LRSFHPLASP LPCDFHLLNL RMLPAEDTLP ATDSALILHR 1090 1100 1110 1120 1130 1140 KGFDCGLEAK NLGFNCTTSQ GKLALGSLFH GLDVTFLQPT SLTLLYPLAS PSNSTDISLE 1150 PMEISTFRLR LG
	Isoform 2 [UniParc]. Checksum: 31A7AC9C5FC4CF2C	FASTA	827	93,450
	10 20 30 40 50 60 MKLKKQVTVC GAAIFCVAVF SLYLMLDRVQ HDPARHQNGG NFPRSQISVL QNRIEQLEQL 70 80 90 100 110 120 LEENHDIISR IKDSVLELTA NAEGPPALLP YHTANGSWAV LPEPRPSFFS VSPQDCQFAL 130 140 150 160 170 180 GGRGQKPELQ MLTVSEDLPF DNVEGGVWRQ GFDISYSPND WDTEDLQVFV VPHSHNDPGW 190 200 210 220 230 240 IKTFDKYYTE QTQHILNSMV SKLQEDPRRR FLWAEVSFFA KWWDNISAQK RAAVRRLVGN 250 260 270 280 290 300 GQLEIATGGW VMPDEANSHY FALVDQLIEG HQWLERNLGA TPRSGWAVDP FGHSSTMPYL 310 320 330 340 350 360 LRRANLTSML IQRVHYAIKK HFAATHSLEF MWRQMWDSDS STDIFCHMMP FYSYDVPHTC 370 380 390 400 410 420 GPDPKICCQF DFKRLPGGRI NCPWKVPPRA ITEANVADRA ALLLDQYRKK SRLFRSNVLL 430 440 450 460 470 480 VPLGDDFRYD KPQEWDAQFF NYQRLFDFLN SKPEFHVQAQ FGTLSEYFDA LYKRTGVEPG 490 500 510 520 530 540 ARPPGFPVLS GDFFSYADRE DHYWTGYYTS RPFYKSLDRV LEAHLRGAEI LYSLALAHAR 550 560 570 580 590 600 RSGLAGQYPL SDFALLTEAR RTLGLFQHHD AITGTAKEAV VVDYGVRLLR SLVSLKQVII 610 620 630 640 650 660 NAAHYLVLGD QETYSFDPGT PFLQMDDSRV SHDALPERTV IRLDSSPRFV VVFNPLEQER 670 680 690 700 710 720 LSVVSLLVNS PRVRVLSEEG QPLSVQISVH WSSATDMVPD VYQVSVPVRL PGLGLGVLQL 730 740 750 760 770 780 QPDLDGPYTL QSSVRVYLNG VKLSVSRQSA FPVRVVDSGA SDFAISNRYM QVWFSGLTGL 790 800 810 820 LKVKGQARGG GEGPEEGMGV RGLVDSCSCS HVPSCLSPDS QRFGCEH

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Brain cortex, Testis and Urinary bladder.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK029913 mRNA. No translation available. AK044028 mRNA. Translation: BAC31745.1. AK137205 mRNA. Translation: BAE23268.1. AC136740 Genomic DNA. No translation available.
IPI	IPI00224294. IPI01027188.
RefSeq	NP_766491.2. NM_172903.4.
UniGene	Mm.269245.
3D structure databases
ProteinModelPortal	Q8BRK9.
SMR	Q8BRK9. Positions 762-1014.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000095949.
Protein family/group databases
CAZy	GH38. Glycoside Hydrolase Family 38.
Proteomic databases
PRIDE	Q8BRK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000098346; ENSMUSP00000095949; ENSMUSG00000038886.
GeneID	140481.
KEGG	mmu:140481.
UCSC	uc009iap.2. mouse. uc009iar.2. mouse.
Organism-specific databases
CTD	4122.
MGI	MGI:2150656. Man2a2.
Phylogenomic databases
GeneTree	ENSGT00510000046304.
HOVERGEN	HBG052390.
InParanoid	Q3UVK1.
KO	K01231.
OMA	FYPMPVM.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	Q8BRK9.
Bgee	Q8BRK9.
Genevestigator	Q8BRK9.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.110.10. 1 hit.
InterPro	IPR011013. Gal_mutarotase_SF_dom. IPR011330. Glyco_hydro/deAcase_b/a-brl. IPR013780. Glyco_hydro_13_b. IPR027291. Glyco_hydro_38/57_N. IPR011682. Glyco_hydro_38_C. IPR015341. Glyco_hydro_38_cen_dom. IPR000602. Glyco_hydro_38_N. [Graphical view]
Pfam	PF09261. Alpha-mann_mid. 1 hit. PF01074. Glyco_hydro_38. 1 hit. PF07748. Glyco_hydro_38C. 1 hit. [Graphical view]
SMART	SM00872. Alpha-mann_mid. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit. SSF88713. Glyco_hydro/deAcase_b/a-brl. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	MAN2A2. mouse.
NextBio	369778.
SOURCE	Search...

Entry information

Entry name

MA2A2_MOUSE

Accession

Primary (citable) accession number: Q8BRK9
Secondary accession number(s): Q3UVK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	September 21, 2011
Last modified:	May 29, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program