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Q8BRK9 (MA2A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-mannosidase 2x

EC=3.2.1.114
Alternative name(s):
Alpha-mannosidase IIx
Short name=Man IIx
Mannosidase alpha class 2A member 2
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene names
Name:Man2a2
Synonyms:Mana2x
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1152 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway By similarity.

Catalytic activity

Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 38 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BRK9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BRK9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     783-827: SIRRVDEEQE...PDSEAKPYVP → VKGQARGGGE...PDSQRFGCEH
     828-1152: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11521152Alpha-mannosidase 2x
PRO_0000412634

Regions

Topological domain1 – 55Cytoplasmic Potential
Transmembrane6 – 2621Helical; Signal-anchor for type II membrane protein; Potential
Topological domain27 – 796770Lumenal Potential
Coiled coil43 – 7432 Potential

Sites

Active site2891Nucleophile By similarity
Metal binding1751Zinc By similarity
Metal binding1771Zinc By similarity
Metal binding2891Zinc By similarity
Metal binding5691Zinc By similarity

Amino acid modifications

Glycosylation2251N-linked (GlcNAc...) Potential
Glycosylation3051N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence783 – 82745SIRRV…KPYVP → VKGQARGGGEGPEEGMGVRG LVDSCSCSHVPSCLSPDSQR FGCEH in isoform 2.
VSP_041735
Alternative sequence828 – 1152325Missing in isoform 2.
VSP_041736

Experimental info

Sequence conflict971S → T in AK029913. Ref.1
Sequence conflict11111G → S in BAE23268. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 8A6274E9671F92DF

FASTA1,152130,649
        10         20         30         40         50         60 
MKLKKQVTVC GAAIFCVAVF SLYLMLDRVQ HDPARHQNGG NFPRSQISVL QNRIEQLEQL 

        70         80         90        100        110        120 
LEENHDIISR IKDSVLELTA NAEGPPALLP YHTANGSWAV LPEPRPSFFS VSPQDCQFAL 

       130        140        150        160        170        180 
GGRGQKPELQ MLTVSEDLPF DNVEGGVWRQ GFDISYSPND WDTEDLQVFV VPHSHNDPGW 

       190        200        210        220        230        240 
IKTFDKYYTE QTQHILNSMV SKLQEDPRRR FLWAEVSFFA KWWDNISAQK RAAVRRLVGN 

       250        260        270        280        290        300 
GQLEIATGGW VMPDEANSHY FALVDQLIEG HQWLERNLGA TPRSGWAVDP FGHSSTMPYL 

       310        320        330        340        350        360 
LRRANLTSML IQRVHYAIKK HFAATHSLEF MWRQMWDSDS STDIFCHMMP FYSYDVPHTC 

       370        380        390        400        410        420 
GPDPKICCQF DFKRLPGGRI NCPWKVPPRA ITEANVADRA ALLLDQYRKK SRLFRSNVLL 

       430        440        450        460        470        480 
VPLGDDFRYD KPQEWDAQFF NYQRLFDFLN SKPEFHVQAQ FGTLSEYFDA LYKRTGVEPG 

       490        500        510        520        530        540 
ARPPGFPVLS GDFFSYADRE DHYWTGYYTS RPFYKSLDRV LEAHLRGAEI LYSLALAHAR 

       550        560        570        580        590        600 
RSGLAGQYPL SDFALLTEAR RTLGLFQHHD AITGTAKEAV VVDYGVRLLR SLVSLKQVII 

       610        620        630        640        650        660 
NAAHYLVLGD QETYSFDPGT PFLQMDDSRV SHDALPERTV IRLDSSPRFV VVFNPLEQER 

       670        680        690        700        710        720 
LSVVSLLVNS PRVRVLSEEG QPLSVQISVH WSSATDMVPD VYQVSVPVRL PGLGLGVLQL 

       730        740        750        760        770        780 
QPDLDGPYTL QSSVRVYLNG VKLSVSRQSA FPVRVVDSGA SDFAISNRYM QVWFSGLTGL 

       790        800        810        820        830        840 
LKSIRRVDEE QEQQMELEFL VYGTRTSKDK SGAYLFLPDS EAKPYVPKKP PVLRVTEGPF 

       850        860        870        880        890        900 
FSEVAVYYEH FHQVIRLYNL PGVEGLSLDM SFQVDIRDYV NKELALRIHT DIDSQGTFFT 

       910        920        930        940        950        960 
DLNGFQIQPR QYLKKLPLQA NFYPMPVMAY IQDSQRRLTL HTAQALGVSS LGNGQLEVIL 

       970        980        990       1000       1010       1020 
DRRLMQDDNR GLGQGLKDNK ITCNRFRLLL ERRTTMSPEV HQEQERSTSY PSLLSHLTSM 

      1030       1040       1050       1060       1070       1080 
YLSTPPLVLP VAKRQGTSPA LRSFHPLASP LPCDFHLLNL RMLPAEDTLP ATDSALILHR 

      1090       1100       1110       1120       1130       1140 
KGFDCGLEAK NLGFNCTTSQ GKLALGSLFH GLDVTFLQPT SLTLLYPLAS PSNSTDISLE 

      1150 
PMEISTFRLR LG 

« Hide

Isoform 2 [UniParc].

Checksum: 31A7AC9C5FC4CF2C
Show »

FASTA82793,450

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Brain cortex, Testis and Urinary bladder.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK029913 mRNA. No translation available.
AK044028 mRNA. Translation: BAC31745.1.
AK137205 mRNA. Translation: BAE23268.1.
AC136740 Genomic DNA. No translation available.
CCDSCCDS57556.1. [Q8BRK9-1]
RefSeqNP_766491.2. NM_172903.4. [Q8BRK9-1]
XP_006540661.1. XM_006540598.1. [Q8BRK9-1]
XP_006540662.1. XM_006540599.1. [Q8BRK9-1]
UniGeneMm.269245.

3D structure databases

ProteinModelPortalQ8BRK9.
SMRQ8BRK9. Positions 762-1014.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000095949.

Protein family/group databases

CAZyGH38. Glycoside Hydrolase Family 38.

Proteomic databases

PRIDEQ8BRK9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000098346; ENSMUSP00000095949; ENSMUSG00000038886. [Q8BRK9-1]
GeneID140481.
KEGGmmu:140481.
UCSCuc009iap.2. mouse. [Q8BRK9-1]
uc009iar.2. mouse. [Q8BRK9-2]

Organism-specific databases

CTD4122.
MGIMGI:2150656. Man2a2.

Phylogenomic databases

GeneTreeENSGT00510000046304.
HOVERGENHBG052390.
InParanoidQ3UVK1.
KOK01231.
OMAYLEPMEI.
OrthoDBEOG7JQBMJ.
PhylomeDBQ8BRK9.
TreeFamTF313152.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ8BRK9.
BgeeQ8BRK9.
GenevestigatorQ8BRK9.

Family and domain databases

Gene3D1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNetSearch...

Other

ChiTaRSMAN2A2. mouse.
NextBio369778.
PROQ8BRK9.
SOURCESearch...

Entry information

Entry nameMA2A2_MOUSE
AccessionPrimary (citable) accession number: Q8BRK9
Secondary accession number(s): Q3UVK1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: July 9, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries