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Q8BRK9

- MA2A2_MOUSE

UniProt

Q8BRK9 - MA2A2_MOUSE

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Protein
Alpha-mannosidase 2x
Gene
Man2a2, Mana2x
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway By similarity.

Catalytic activityi

Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi175 – 1751Zinc By similarity
Metal bindingi177 – 1771Zinc By similarity
Active sitei289 – 2891Nucleophile By similarity
Metal bindingi289 – 2891Zinc By similarity
Metal bindingi569 – 5691Zinc By similarity

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. hydrolase activity, hydrolyzing N-glycosyl compounds Source: MGI
  3. mannosidase activity Source: MGI
  4. mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activity Source: UniProtKB-EC
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. mannose metabolic process Source: InterPro
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_198568. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGH38. Glycoside Hydrolase Family 38.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-mannosidase 2x (EC:3.2.1.114)
Alternative name(s):
Alpha-mannosidase IIx
Short name:
Man IIx
Mannosidase alpha class 2A member 2
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
Gene namesi
Name:Man2a2
Synonyms:Mana2x
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2150656. Man2a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55Cytoplasmic Reviewed prediction
Transmembranei6 – 2621Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini27 – 796770Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11521152Alpha-mannosidase 2x
PRO_0000412634Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi225 – 2251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi305 – 3051N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8BRK9.

Expressioni

Gene expression databases

ArrayExpressiQ8BRK9.
BgeeiQ8BRK9.
GenevestigatoriQ8BRK9.

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095949.

Structurei

3D structure databases

ProteinModelPortaliQ8BRK9.
SMRiQ8BRK9. Positions 762-1014.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili43 – 7432 Reviewed prediction
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00510000046304.
HOVERGENiHBG052390.
InParanoidiQ3UVK1.
KOiK01231.
OMAiYLEPMEI.
OrthoDBiEOG7JQBMJ.
PhylomeDBiQ8BRK9.
TreeFamiTF313152.

Family and domain databases

Gene3Di1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProiIPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view]
PfamiPF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view]
SMARTiSM00872. Alpha-mann_mid. 1 hit.
[Graphical view]
SUPFAMiSSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BRK9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKLKKQVTVC GAAIFCVAVF SLYLMLDRVQ HDPARHQNGG NFPRSQISVL     50
QNRIEQLEQL LEENHDIISR IKDSVLELTA NAEGPPALLP YHTANGSWAV 100
LPEPRPSFFS VSPQDCQFAL GGRGQKPELQ MLTVSEDLPF DNVEGGVWRQ 150
GFDISYSPND WDTEDLQVFV VPHSHNDPGW IKTFDKYYTE QTQHILNSMV 200
SKLQEDPRRR FLWAEVSFFA KWWDNISAQK RAAVRRLVGN GQLEIATGGW 250
VMPDEANSHY FALVDQLIEG HQWLERNLGA TPRSGWAVDP FGHSSTMPYL 300
LRRANLTSML IQRVHYAIKK HFAATHSLEF MWRQMWDSDS STDIFCHMMP 350
FYSYDVPHTC GPDPKICCQF DFKRLPGGRI NCPWKVPPRA ITEANVADRA 400
ALLLDQYRKK SRLFRSNVLL VPLGDDFRYD KPQEWDAQFF NYQRLFDFLN 450
SKPEFHVQAQ FGTLSEYFDA LYKRTGVEPG ARPPGFPVLS GDFFSYADRE 500
DHYWTGYYTS RPFYKSLDRV LEAHLRGAEI LYSLALAHAR RSGLAGQYPL 550
SDFALLTEAR RTLGLFQHHD AITGTAKEAV VVDYGVRLLR SLVSLKQVII 600
NAAHYLVLGD QETYSFDPGT PFLQMDDSRV SHDALPERTV IRLDSSPRFV 650
VVFNPLEQER LSVVSLLVNS PRVRVLSEEG QPLSVQISVH WSSATDMVPD 700
VYQVSVPVRL PGLGLGVLQL QPDLDGPYTL QSSVRVYLNG VKLSVSRQSA 750
FPVRVVDSGA SDFAISNRYM QVWFSGLTGL LKSIRRVDEE QEQQMELEFL 800
VYGTRTSKDK SGAYLFLPDS EAKPYVPKKP PVLRVTEGPF FSEVAVYYEH 850
FHQVIRLYNL PGVEGLSLDM SFQVDIRDYV NKELALRIHT DIDSQGTFFT 900
DLNGFQIQPR QYLKKLPLQA NFYPMPVMAY IQDSQRRLTL HTAQALGVSS 950
LGNGQLEVIL DRRLMQDDNR GLGQGLKDNK ITCNRFRLLL ERRTTMSPEV 1000
HQEQERSTSY PSLLSHLTSM YLSTPPLVLP VAKRQGTSPA LRSFHPLASP 1050
LPCDFHLLNL RMLPAEDTLP ATDSALILHR KGFDCGLEAK NLGFNCTTSQ 1100
GKLALGSLFH GLDVTFLQPT SLTLLYPLAS PSNSTDISLE PMEISTFRLR 1150
LG 1152
Length:1,152
Mass (Da):130,649
Last modified:September 21, 2011 - v2
Checksum:i8A6274E9671F92DF
GO
Isoform 2 (identifier: Q8BRK9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     783-827: SIRRVDEEQE...PDSEAKPYVP → VKGQARGGGE...PDSQRFGCEH
     828-1152: Missing.

Show »
Length:827
Mass (Da):93,450
Checksum:i31A7AC9C5FC4CF2C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei783 – 82745SIRRV…KPYVP → VKGQARGGGEGPEEGMGVRG LVDSCSCSHVPSCLSPDSQR FGCEH in isoform 2.
VSP_041735Add
BLAST
Alternative sequencei828 – 1152325Missing in isoform 2.
VSP_041736Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti97 – 971S → T in AK029913. 1 Publication
Sequence conflicti1111 – 11111G → S in BAE23268. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK029913 mRNA. No translation available.
AK044028 mRNA. Translation: BAC31745.1.
AK137205 mRNA. Translation: BAE23268.1.
AC136740 Genomic DNA. No translation available.
CCDSiCCDS57556.1. [Q8BRK9-1]
RefSeqiNP_766491.2. NM_172903.4. [Q8BRK9-1]
XP_006540661.1. XM_006540598.1. [Q8BRK9-1]
XP_006540662.1. XM_006540599.1. [Q8BRK9-1]
UniGeneiMm.269245.

Genome annotation databases

EnsembliENSMUST00000098346; ENSMUSP00000095949; ENSMUSG00000038886. [Q8BRK9-1]
GeneIDi140481.
KEGGimmu:140481.
UCSCiuc009iap.2. mouse. [Q8BRK9-1]
uc009iar.2. mouse. [Q8BRK9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK029913 mRNA. No translation available.
AK044028 mRNA. Translation: BAC31745.1 .
AK137205 mRNA. Translation: BAE23268.1 .
AC136740 Genomic DNA. No translation available.
CCDSi CCDS57556.1. [Q8BRK9-1 ]
RefSeqi NP_766491.2. NM_172903.4. [Q8BRK9-1 ]
XP_006540661.1. XM_006540598.1. [Q8BRK9-1 ]
XP_006540662.1. XM_006540599.1. [Q8BRK9-1 ]
UniGenei Mm.269245.

3D structure databases

ProteinModelPortali Q8BRK9.
SMRi Q8BRK9. Positions 762-1014.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000095949.

Protein family/group databases

CAZyi GH38. Glycoside Hydrolase Family 38.

Proteomic databases

PRIDEi Q8BRK9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000098346 ; ENSMUSP00000095949 ; ENSMUSG00000038886 . [Q8BRK9-1 ]
GeneIDi 140481.
KEGGi mmu:140481.
UCSCi uc009iap.2. mouse. [Q8BRK9-1 ]
uc009iar.2. mouse. [Q8BRK9-2 ]

Organism-specific databases

CTDi 4122.
MGIi MGI:2150656. Man2a2.

Phylogenomic databases

GeneTreei ENSGT00510000046304.
HOVERGENi HBG052390.
InParanoidi Q3UVK1.
KOi K01231.
OMAi YLEPMEI.
OrthoDBi EOG7JQBMJ.
PhylomeDBi Q8BRK9.
TreeFami TF313152.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_198568. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

ChiTaRSi MAN2A2. mouse.
NextBioi 369778.
PROi Q8BRK9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8BRK9.
Bgeei Q8BRK9.
Genevestigatori Q8BRK9.

Family and domain databases

Gene3Di 1.20.1270.50. 1 hit.
2.60.40.1180. 1 hit.
3.20.110.10. 1 hit.
InterProi IPR011013. Gal_mutarotase_SF_dom.
IPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR013780. Glyco_hydro_13_b.
IPR027291. Glyco_hydro_38/57_N.
IPR011682. Glyco_hydro_38_C.
IPR015341. Glyco_hydro_38_cen.
IPR000602. Glyco_hydro_38_N.
IPR028995. Glyco_hydro_57/38_cen.
[Graphical view ]
Pfami PF09261. Alpha-mann_mid. 1 hit.
PF01074. Glyco_hydro_38. 1 hit.
PF07748. Glyco_hydro_38C. 1 hit.
[Graphical view ]
SMARTi SM00872. Alpha-mann_mid. 1 hit.
[Graphical view ]
SUPFAMi SSF74650. SSF74650. 1 hit.
SSF88688. SSF88688. 1 hit.
SSF88713. SSF88713. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Brain cortex, Testis and Urinary bladder.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiMA2A2_MOUSE
AccessioniPrimary (citable) accession number: Q8BRK9
Secondary accession number(s): Q3UVK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: September 3, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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