##gff-version 3
Q8BRK8	UniProtKB	Chain	1	552	.	.	.	ID=PRO_0000262957;Note=5'-AMP-activated protein kinase catalytic subunit alpha-2	
Q8BRK8	UniProtKB	Domain	16	268	.	.	.	Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159	
Q8BRK8	UniProtKB	Region	291	376	.	.	.	Note=AIS;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q13131	
Q8BRK8	UniProtKB	Active site	139	139	.	.	.	Note=Proton acceptor;Ontology_term=ECO:0000250,ECO:0000255,ECO:0000255;evidence=ECO:0000250|UniProtKB:P28523,ECO:0000255|PROSITE-ProRule:PRU00159,ECO:0000255|PROSITE-ProRule:PRU10027	
Q8BRK8	UniProtKB	Binding site	22	30	.	.	.	Ontology_term=ECO:0000250,ECO:0000255;evidence=ECO:0000250|UniProtKB:P28523,ECO:0000255|PROSITE-ProRule:PRU00159	
Q8BRK8	UniProtKB	Binding site	45	45	.	.	.	.	
Q8BRK8	UniProtKB	Modified residue	172	172	.	.	.	Note=Phosphothreonine%3B by LKB1 and CaMKK2;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:15980064,ECO:0000269|PubMed:16308421,ECO:0000269|PubMed:23332761,ECO:0000269|PubMed:30201684;Dbxref=PMID:15980064,PMID:16308421,PMID:23332761,PMID:30201684	
Q8BRK8	UniProtKB	Modified residue	258	258	.	.	.	Note=Phosphothreonine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q09137	
Q8BRK8	UniProtKB	Modified residue	377	377	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:17242355,ECO:0007744|PubMed:21183079;Dbxref=PMID:17242355,PMID:21183079	
Q8BRK8	UniProtKB	Modified residue	491	491	.	.	.	Note=Phosphoserine;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q09137	
Q8BRK8	UniProtKB	Mutagenesis	45	45	.	.	.	Note=Loss of kinase activity. K->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21454484;Dbxref=PMID:21454484	
Q8BRK8	UniProtKB	Mutagenesis	157	157	.	.	.	Note=Loss of kinase activity. D->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:20647423;Dbxref=PMID:20647423	
Q8BRK8	UniProtKB	Sequence conflict	15	15	.	.	.	Note=H->D;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q8BRK8	UniProtKB	Sequence conflict	289	289	.	.	.	Note=D->V;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q8BRK8	UniProtKB	Sequence conflict	380	380	.	.	.	Note=A->E;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q8BRK8	UniProtKB	Sequence conflict	502	502	.	.	.	Note=F->Y;Ontology_term=ECO:0000305;evidence=ECO:0000305	
Q8BRK8	UniProtKB	Sequence conflict	506	506	.	.	.	Note=T->K;Ontology_term=ECO:0000305;evidence=ECO:0000305