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Protein

Putative nuclease HARBI1

Gene

Harbi1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transposase-derived protein that may have nuclease activity (Potential). Does not have transposase activity (By similarity).By similarityCurated

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi149Divalent metal cationSequence analysis1
Metal bindingi199Divalent metal cationSequence analysis1
Metal bindingi225Divalent metal cationSequence analysis1
Metal bindingi261Divalent metal cationSequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative nuclease HARBI1 (EC:3.1.-.-)
Alternative name(s):
Harbinger transposase-derived nuclease
Gene namesi
Name:Harbi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2443194. Harbi1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Interaction with NAIF1 promotes translocation to the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002636151 – 349Putative nuclease HARBI1Add BLAST349

Proteomic databases

PaxDbiQ8BR93.
PRIDEiQ8BR93.

PTM databases

PhosphoSitePlusiQ8BR93.

Expressioni

Tissue specificityi

Detected in adult brain, eye, nerve tissue and lung. Detected in embryo.1 Publication

Gene expression databases

BgeeiENSMUSG00000027243.
ExpressionAtlasiQ8BR93. baseline and differential.
GenevisibleiQ8BR93. MM.

Interactioni

Subunit structurei

Interacts with NAIF1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000088098.

Structurei

3D structure databases

ProteinModelPortaliQ8BR93.
SMRiQ8BR93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 300DDE Tnp4Sequence analysisAdd BLAST152

Sequence similaritiesi

Belongs to the HARBI1 family.Curated
Contains 1 DDE Tnp4 domain.Sequence analysis

Phylogenomic databases

eggNOGiKOG4585. Eukaryota.
ENOG411206Y. LUCA.
GeneTreeiENSGT00530000063045.
HOGENOMiHOG000231449.
HOVERGENiHBG054543.
InParanoidiQ8BR93.
OMAiAHSATHN.
OrthoDBiEOG091G0APR.
PhylomeDBiQ8BR93.
TreeFamiTF327972.

Family and domain databases

InterProiIPR026103. HARBI1_animal.
IPR027806. HARBI1_dom.
[Graphical view]
PANTHERiPTHR22930:SF36. PTHR22930:SF36. 1 hit.
PfamiPF13359. DDE_Tnp_4. 1 hit.
[Graphical view]
PRINTSiPR02086. PUTNUCHARBI1.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BR93-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIPITVLDC DLLLYGRGHR TLDRFKLDDV TDEYLMSMYG FPRQFIYFLV
60 70 80 90 100
ELLGASLSRP TQRSRAISPE TQILAALGFY TSGSFQTRMG DAIGISQASM
110 120 130 140 150
SRCVANVTEA LVERASQFIH FPVDEAAVQS LKDEFYGLAG MPGVIGVADC
160 170 180 190 200
IHVAIKAPNA EDLSYVNRKG LHSLNCLVVC DIRGALMTVE TSWPGSLQDC
210 220 230 240 250
AVLQRSSLTS QFETGMPKDS WLLGDSSFFL RSWLLTPLPI PETAAEYRYN
260 270 280 290 300
RAHSATHSVI ERTLQTLCCR FRCLDGSKGA LQYSPEKCSH IILACCVLHN
310 320 330 340
ISLDHGMDVW SSPVPGPIDQ PPEGEDEHME SLDLEADRIR QELILTHFS
Length:349
Mass (Da):38,713
Last modified:March 1, 2003 - v1
Checksum:iBA1CCEB600A46010
GO
Isoform 2 (identifier: Q8BR93-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-245: DSSFFLRSWLLTPLPIPETAA → CATQALLYDCSVRESSAPES
     246-349: Missing.

Note: No experimental confirmation available.
Show »
Length:244
Mass (Da):26,681
Checksum:iF5FC5A7911D7E81C
GO
Isoform 3 (identifier: Q8BR93-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-246: DSSFFLRSWLLTPLPIPETAAE → KFASINFQFMSIEKLCRRVGEI
     247-349: Missing.

Note: No experimental confirmation available.
Show »
Length:246
Mass (Da):27,167
Checksum:iCFB773AB600AD165
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272R → Q in BAC37974 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021876225 – 246DSSFF…ETAAE → KFASINFQFMSIEKLCRRVG EI in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_021877225 – 245DSSFF…PETAA → CATQALLYDCSVRESSAPES in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_021878246 – 349Missing in isoform 2. 1 PublicationAdd BLAST104
Alternative sequenceiVSP_021879247 – 349Missing in isoform 3. 1 PublicationAdd BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041747 mRNA. Translation: BAC31051.1.
AK045343 mRNA. Translation: BAC32318.1.
AK080671 mRNA. Translation: BAC37974.1.
AK084226 mRNA. Translation: BAC39145.1.
AK147045 mRNA. Translation: BAE27631.1.
AL714023 Genomic DNA. Translation: CAM14514.1.
AL714023 Genomic DNA. Translation: CAX15352.1.
BC094315 mRNA. Translation: AAH94315.1.
CCDSiCCDS50642.1. [Q8BR93-1]
RefSeqiNP_848839.2. NM_178724.4. [Q8BR93-1]
XP_006499533.1. XM_006499470.1. [Q8BR93-1]
XP_006499534.1. XM_006499471.2. [Q8BR93-1]
XP_017173596.1. XM_017318107.1. [Q8BR93-2]
UniGeneiMm.130331.

Genome annotation databases

EnsembliENSMUST00000090608; ENSMUSP00000088098; ENSMUSG00000027243. [Q8BR93-1]
ENSMUST00000111322; ENSMUSP00000106954; ENSMUSG00000027243. [Q8BR93-2]
ENSMUST00000142692; ENSMUSP00000121420; ENSMUSG00000027243. [Q8BR93-3]
GeneIDi241547.
KEGGimmu:241547.
UCSCiuc008kwo.1. mouse. [Q8BR93-3]
uc008kwp.1. mouse. [Q8BR93-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041747 mRNA. Translation: BAC31051.1.
AK045343 mRNA. Translation: BAC32318.1.
AK080671 mRNA. Translation: BAC37974.1.
AK084226 mRNA. Translation: BAC39145.1.
AK147045 mRNA. Translation: BAE27631.1.
AL714023 Genomic DNA. Translation: CAM14514.1.
AL714023 Genomic DNA. Translation: CAX15352.1.
BC094315 mRNA. Translation: AAH94315.1.
CCDSiCCDS50642.1. [Q8BR93-1]
RefSeqiNP_848839.2. NM_178724.4. [Q8BR93-1]
XP_006499533.1. XM_006499470.1. [Q8BR93-1]
XP_006499534.1. XM_006499471.2. [Q8BR93-1]
XP_017173596.1. XM_017318107.1. [Q8BR93-2]
UniGeneiMm.130331.

3D structure databases

ProteinModelPortaliQ8BR93.
SMRiQ8BR93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000088098.

PTM databases

PhosphoSitePlusiQ8BR93.

Proteomic databases

PaxDbiQ8BR93.
PRIDEiQ8BR93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090608; ENSMUSP00000088098; ENSMUSG00000027243. [Q8BR93-1]
ENSMUST00000111322; ENSMUSP00000106954; ENSMUSG00000027243. [Q8BR93-2]
ENSMUST00000142692; ENSMUSP00000121420; ENSMUSG00000027243. [Q8BR93-3]
GeneIDi241547.
KEGGimmu:241547.
UCSCiuc008kwo.1. mouse. [Q8BR93-3]
uc008kwp.1. mouse. [Q8BR93-1]

Organism-specific databases

CTDi283254.
MGIiMGI:2443194. Harbi1.

Phylogenomic databases

eggNOGiKOG4585. Eukaryota.
ENOG411206Y. LUCA.
GeneTreeiENSGT00530000063045.
HOGENOMiHOG000231449.
HOVERGENiHBG054543.
InParanoidiQ8BR93.
OMAiAHSATHN.
OrthoDBiEOG091G0APR.
PhylomeDBiQ8BR93.
TreeFamiTF327972.

Miscellaneous databases

ChiTaRSiHarbi1. mouse.
PROiQ8BR93.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027243.
ExpressionAtlasiQ8BR93. baseline and differential.
GenevisibleiQ8BR93. MM.

Family and domain databases

InterProiIPR026103. HARBI1_animal.
IPR027806. HARBI1_dom.
[Graphical view]
PANTHERiPTHR22930:SF36. PTHR22930:SF36. 1 hit.
PfamiPF13359. DDE_Tnp_4. 1 hit.
[Graphical view]
PRINTSiPR02086. PUTNUCHARBI1.
ProtoNetiSearch...

Entry informationi

Entry nameiHARB1_MOUSE
AccessioniPrimary (citable) accession number: Q8BR93
Secondary accession number(s): A2AH19
, B7ZCF8, Q8BNA8, Q8BNT1, Q8C9N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.