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Protein

Kin of IRRE-like protein 3

Gene

Kirrel3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Synaptic adhesion molecule required for the formation of target-specific synapses (PubMed:23637329, PubMed:26575286). Required for formation of target-specific synapses at hippocampal mossy fiber synapses. Required for formation of mossy fiber filopodia, the synaptic structures connecting dentate granule and GABA neurons. Probably acts as a homophilic adhesion molecule that promotes trans-cellular interactions and stabilize mossy fiber filipodia contact and subsequent synapse formation (PubMed:26575286). Required for the coalescence of vomeronasal sensory neuron axons (PubMed:23637329). May be involved in the hematopoietic supportive capacity of stroma cells; the secreted extracellular domain is directly responsible for supporting hematopoietic stem cells (PubMed:12665856).3 Publications

GO - Molecular functioni

  • PDZ domain binding Source: UniProtKB

GO - Biological processi

  • glomerulus morphogenesis Source: MGI
  • hemopoiesis Source: UniProtKB
  • hippocampus development Source: UniProtKB
  • homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
  • inter-male aggressive behavior Source: MGI
  • neuron migration Source: MGI
  • neuron projection morphogenesis Source: MGI
  • principal sensory nucleus of trigeminal nerve development Source: MGI
  • synapse assembly Source: UniProtKB

Keywordsi

Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-373753. Nephrin family interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Kin of IRRE-like protein 3Curated
Alternative name(s):
Kin of irregular chiasm-like protein 3
Nephrin-like protein 21 Publication
mKirre1 Publication
Cleaved into the following chain:
Gene namesi
Name:Kirrel3
Synonyms:Kiaa1867, Neph21 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1914953. Kirrel3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 535ExtracellularSequence analysisAdd BLAST514
Transmembranei536 – 556HelicalSequence analysisAdd BLAST21
Topological domaini557 – 778CytoplasmicSequence analysisAdd BLAST222

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Mice are viable and their size is normal. Mice show defects of the accessory olfactory system characterized by disorganization of the glomerular layer of the posterior accessory olfactory bulb and formation of fewer, larger glomeruli. Mice display a loss of male-male aggression in a resident-intruder assay (PubMed:23637329). Mice display moderate hyperactivity in a familiar, but not novel, environment and defective novel object recognition with normal performances in Morris water maze spatial learning and memory, contextual fear conditioning and extinction, and pattern separation tests (PubMed:26283919). Young mutant mice form fewer synapse-forming structures between dentate granule neurons and GABAergic neurons than normal mice, while the synapses that connect dentate granule neurons to CA3 neurons form normally. This may affect the balance of activity across the two types of dentate granule synapses and the CA3 neurons, leading to hyperactivity (PubMed:26575286).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001509922 – 778Kin of IRRE-like protein 3Add BLAST757
ChainiPRO_000029624122 – ?Processed kin of IRRE-like protein 31 Publication

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi69 ↔ 127PROSITE-ProRule annotation
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi170 ↔ 227PROSITE-ProRule annotation
Glycosylationi253N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi271 ↔ 314PROSITE-ProRule annotation
Glycosylationi324N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi356 ↔ 398PROSITE-ProRule annotation
Disulfide bondi440 ↔ 499PROSITE-ProRule annotation
Glycosylationi498N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Undergoes proteolysis by a metalloprotease and gives rise to a soluble form.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8BR86.
PRIDEiQ8BR86.

PTM databases

iPTMnetiQ8BR86.
PhosphoSitePlusiQ8BR86.

Expressioni

Tissue specificityi

Expressed mainly in adult brain, bone marrow and stromal cells (PubMed:12665856). Expressed in diverse regions of the brain, including the cortex, hippocampus, striatum, olfactory bulb and cerebellum (PubMed:15908127, PubMed:26283919). In brain, expressed in pontine nucleus neurons (at protein level) (PubMed:21241806). In hippocampus, produced in both the dentate granule neurons and the GABAergic neurons, but not the CA3 neurons (PubMed:26575286). Expressed in subpopulations of vomeronasal sensory neurons (PubMed:23637329). Expressed in a subset of neurons in dorsal root ganglia (PubMed:18752272).7 Publications

Developmental stagei

At embryonic day 11.5, it is expressed in the differentiating zones of various regions, such as the caudate-putamen, the geniculate body, the thalamus, the amygdala and the brainstem. This expression persists in the adult, although expression is lower. After birth, highly expressed in the glomerular and mitral layers of the olfactory bulb, the cortical plate of the neocortex, the cochlear nucleus, and the molecular and granule cell layers of the cerebellum. In the hippocampus, expression is first observed in the dentate gyrus at postnatal day 7.1 Publication

Gene expression databases

BgeeiENSMUSG00000032036.
CleanExiMM_KIRREL3.
ExpressionAtlasiQ8BR86. baseline and differential.
GenevisibleiQ8BR86. MM.

Interactioni

Subunit structurei

Homodimer; mediates homophilic interactions to promote cell adhesion (PubMed:26575286). Interacts with NPHS1; forms heterodimers with NPHS1 (PubMed:15843475, PubMed:18752272). Interacts with NPHS2/podocin (via the C-terminus) (By similarity). Interacts with CASK. Interacts (via extracellular region) with MAP1B. Interacts (via extracellular region) with MYO16. Interacts (via intracellular region) with ATP1B1. Interacts (via intracellular region) with SHMT2. Interacts (via intracellular region) with UFC1 (By similarity).By similarity3 Publications

GO - Molecular functioni

  • PDZ domain binding Source: UniProtKB

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110801.

Structurei

3D structure databases

ProteinModelPortaliQ8BR86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 142Ig-like C2-type 1Add BLAST95
Domaini147 – 243Ig-like C2-type 2Add BLAST97
Domaini249 – 330Ig-like C2-type 3Add BLAST82
Domaini335 – 415Ig-like C2-type 4Add BLAST81
Domaini419 – 515Ig-like C2-type 5Add BLAST97

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi701 – 767Ser-richAdd BLAST67

Sequence similaritiesi

Belongs to the immunoglobulin superfamily.Curated

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3510. Eukaryota.
ENOG410XPX0. LUCA.
GeneTreeiENSGT00550000074545.
HOGENOMiHOG000020211.
HOVERGENiHBG052260.
InParanoidiQ8BR86.
OMAiKEQGSEM.
OrthoDBiEOG091G02BJ.
PhylomeDBiQ8BR86.
TreeFamiTF327139.

Family and domain databases

Gene3Di1.20.5.100. 1 hit.
2.60.40.10. 6 hits.
InterProiView protein in InterPro
IPR021157. Cyt_c1_TM_anchor_C.
IPR007110. Ig-like_dom.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
PfamiView protein in Pfam
PF07679. I-set. 2 hits.
PF13895. Ig_2. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 4 hits.
SM00408. IGc2. 3 hits.
SUPFAMiSSF48726. SSF48726. 5 hits.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 5 hits.

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BR86-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRPFQLDLLF LCFFLFSQEL GLQKRGCCLV LGYMAKDKFR RMNEGQVYSF
60 70 80 90 100
SQQPQDQVVV SGQPVTLLCA IPEYDGFVLW IKDGLALGVG RDLSSYPQYL
110 120 130 140 150
VVGNHLSGEH HLKILRAELQ DDAVYECQAI QAAIRSRPAR LTVLVPPDDP
160 170 180 190 200
IILGGPVISL RAGDPLNLTC HADNAKPAAS IIWLRKGEVI NGATYSKTLL
210 220 230 240 250
RDGKRESIVS TLFISPGDVE NGQSIVCRAT NKAIPGGKET SVTIDIQHPP
260 270 280 290 300
LVNLSVEPQP VLEDNIVTFH CSAKANPAVT QYRWAKRGHI IKEASGELYR
310 320 330 340 350
TTVDYTYFSE PVSCEVTNAL GSTNLSRTVD VYFGPRMTSE PQSLLVDLGS
360 370 380 390 400
DAVFSCAWIG NPSLTIVWMK RGSGVVLSNE KTLTLKSVRQ EDAGKYVCRA
410 420 430 440 450
VVPRVGAGER EVTLTVNGPP IISSTQTQHA LHGEKGQIKC FIRSTPPPDR
460 470 480 490 500
IAWSWKENVL ESGTSGRYTV ETVNTEEGVI STLTISNIVR ADFQTIYNCT
510 520 530 540 550
AWNSFGSDTE IIRLKEQGSE MKSGAGLEAE SVPMAVIIGV AVGAGVAFLV
560 570 580 590 600
LMATIVAFCC ARSQRNLKGV VSAKNDIRVE IVHKEPSSGR EAEDHTTIKQ
610 620 630 640 650
LMMDRGEFQQ DSVLKQLEVL KEEEKEFQNL KDPTNGYYSV NTFKEHHSTP
660 670 680 690 700
TISLSSCQPD LRPTGKQRVP TGMSFTNIYS TLSGQGRLYD YGQRFVLGMG
710 720 730 740 750
SSSIELCERE FQRGSLSDSS SFLDTQCDSS VSSSGKQDGY VQFDKASKAS
760 770
ASSSHHSQSS SQNSDPSRPL QRRMQTHV
Length:778
Mass (Da):85,405
Last modified:March 1, 2003 - v1
Checksum:iD8DBEC8C096B4F81
GO
Isoform 2 (identifier: Q8BR86-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     19-44: Missing.
     565-565: R → RSTGGRPGISGRGTEKKARLRLPRRA

Show »
Length:777
Mass (Da):85,023
Checksum:iD5978CE416613397
GO
Isoform 3 (identifier: Q8BR86-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     518-529: Missing.
     603-624: MDRGEFQQDSVLKQLEVLKEEE → VRAQPMPHSILSTQTSRCSPYC
     625-778: Missing.

Show »
Length:612
Mass (Da):67,096
Checksum:i7DACBD0BD8337ADD
GO
Isoform 4 (identifier: Q8BR86-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     518-529: Missing.

Show »
Length:766
Mass (Da):84,287
Checksum:iA4C0FEC141CB2F5C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01181619 – 44Missing in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_011817518 – 529Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST12
Alternative sequenceiVSP_011818565R → RSTGGRPGISGRGTEKKARL RLPRRA in isoform 2. 1 Publication1
Alternative sequenceiVSP_011819603 – 624MDRGE…LKEEE → VRAQPMPHSILSTQTSRCSP YC in isoform 3. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_011820625 – 778Missing in isoform 3. 1 PublicationAdd BLAST154

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY169782 mRNA. Translation: AAO41835.1.
AK034142 mRNA. Translation: BAC28604.1.
AK038385 mRNA. Translation: BAC29980.1.
AK043580 mRNA. Translation: BAC31587.1.
AK045373 mRNA. Translation: BAC32333.1.
BC063072 mRNA. Translation: AAH63072.1.
CCDSiCCDS40574.1. [Q8BR86-4]
CCDS80973.1. [Q8BR86-2]
RefSeqiNP_001177840.1. NM_001190911.1. [Q8BR86-1]
NP_001177841.1. NM_001190912.1. [Q8BR86-2]
NP_001177842.1. NM_001190913.1. [Q8BR86-3]
NP_080600.1. NM_026324.3. [Q8BR86-4]
XP_006510623.1. XM_006510560.3. [Q8BR86-1]
XP_006510624.1. XM_006510561.3. [Q8BR86-2]
XP_006510626.1. XM_006510563.3. [Q8BR86-4]
UniGeneiMm.220710.

Genome annotation databases

EnsembliENSMUST00000115148; ENSMUSP00000110801; ENSMUSG00000032036. [Q8BR86-4]
ENSMUST00000187182; ENSMUSP00000140219; ENSMUSG00000032036. [Q8BR86-2]
GeneIDi67703.
KEGGimmu:67703.
UCSCiuc009osg.1. mouse. [Q8BR86-3]
uc009osh.2. mouse. [Q8BR86-4]
uc009osi.2. mouse. [Q8BR86-1]
uc009osj.2. mouse. [Q8BR86-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKIRR3_MOUSE
AccessioniPrimary (citable) accession number: Q8BR86
Secondary accession number(s): Q6P555
, Q810H3, Q8BGQ5, Q8BRS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: March 1, 2003
Last modified: October 25, 2017
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families