Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ral GTPase-activating protein subunit beta

Gene

Ralgapb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic subunit of the heterodimeric RalGAP1 and RalGAP2 complexes which act as GTPase activators for the Ras-like small GTPases RALA and RALB.By similarity

GO - Molecular functioni

GO - Biological processi

  • activation of GTPase activity Source: UniProtKB
  • membrane organization Source: Reactome
  • Ral protein signal transduction Source: MGI
  • regulation of exocyst localization Source: MGI
  • regulation of protein localization Source: MGI
  • regulation of small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Ral GTPase-activating protein subunit beta
Alternative name(s):
p170
Gene namesi
Name:Ralgapb
Synonyms:Kiaa1219
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2444531. Ralgapb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14841484Ral GTPase-activating protein subunit betaPRO_0000056757Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei363 – 3631PhosphothreonineBy similarity
Modified residuei379 – 3791PhosphothreonineCombined sources
Modified residuei421 – 4211PhosphoserineCombined sources
Modified residuei724 – 7241PhosphothreonineCombined sources
Modified residuei1275 – 12751PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8BQZ4.
MaxQBiQ8BQZ4.
PaxDbiQ8BQZ4.
PRIDEiQ8BQZ4.

PTM databases

iPTMnetiQ8BQZ4.
PhosphoSiteiQ8BQZ4.

Expressioni

Tissue specificityi

Abundantly expressed in testis, pancreas, lung, thymus, brown fat, and white fat. Expressed at lower levels in the brain.1 Publication

Gene expression databases

CleanExiMM_B230339M05RIK.

Interactioni

Subunit structurei

Component of the heterodimeric RalGAP1 complex with RALGAPA1 and of the heterodimeric RalGAP2 complex with RALGAPA2. Heterodimerization is required for activity (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048430.

Structurei

3D structure databases

ProteinModelPortaliQ8BQZ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1138 – 1382245Rap-GAPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Rap-GAP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3652. Eukaryota.
ENOG411027C. LUCA.
HOGENOMiHOG000230877.
HOVERGENiHBG052199.
InParanoidiQ8BQZ4.
PhylomeDBiQ8BQZ4.

Family and domain databases

InterProiIPR000331. Rap_GAP_dom.
[Graphical view]
SUPFAMiSSF111347. SSF111347. 3 hits.
PROSITEiPS50085. RAPGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BQZ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYSEWRSLHL VIQSDQGHTS VLHSYPESVG REVANAVVRP LGQALGTSSV
60 70 80 90 100
AGSESLLKTD KEVKWTMEVI CYGLTLPLDG ETVKYCVDVY TDWIMALVLP
110 120 130 140 150
KDSIPLPIIK EPNLYVQSIL KHLQNLFVPR QEQGSSQIRL CLQVLRAIQK
160 170 180 190 200
LARESSIMAR ETWEVLLLFL LQINDILLAP PTVQGGIAEN LAEKLIGVLF
210 220 230 240 250
EVWLLACTRC FPTPPYWKTA KEMVANWRHH PAVVEQWSKV ICALTSRLLR
260 270 280 290 300
FTYGPSFPPF KVPDEDANLI PPEMDNECIA QTWFRFLHML SNPVDLSNPA
310 320 330 340 350
VISSTPKFQE QFLNVSGMPQ ELSQYPCLKH LPQIFFRAMR GISCLVDAFL
360 370 380 390 400
GISRPRSDSA PPTPVNRLSM PQSAAVNTTP PHNRRHRAVT VNKATMKTST
410 420 430 440 450
VTTAHTSKVQ HQASSTSPLS SPNQTSSEPR PLPAPRRPKV NSILNLFGSW
460 470 480 490 500
LFDAAFVHCK LHNGINRDNS MTASFIQILL SYKSSIATQA SMEFRRKGSQ
510 520 530 540 550
MSTDTMVSNP VFDASEFPDN YEAGRAEACG TLCRIFCSKK TGEEILPAYL
560 570 580 590 600
SSVILNSPPL FCCDLKGIDV VVPYFISALE TILPDRELSK FKSYVNPTEL
610 620 630 640 650
RRSSINILLS LLPLPHHFGT VRSEVVLEGK FSNDDSSSYD KPITFLSLKL
660 670 680 690 700
RLVNILIGAL QTETDPNNTQ MILGAMLNIV QDSALLEAIG CQMEMGGGEN
710 720 730 740 750
NLKSHSRTNS GISSASGGST EPTTPDSERP AQALLRDYGS TDSAAGLLIR
760 770 780 790 800
SIHLVTQRLN SQWRQDMSIS LAALELLSGL AKVKVMVDSG DRKRAISSVC
810 820 830 840 850
SYIVYQCSRP APLHSRDLHS MIVAAFQCLC VWLTEHPDML DEKDCLKEVL
860 870 880 890 900
EIVELGISGS KSKNSEQEVK YKGDKEPNPA SMRVKDAAEA TLTCIMQLLG
910 920 930 940 950
AFPSPSGPAS PCSLVNETTL IKYSRLPTIN KHSFRYFVLD NSVILAMLEQ
960 970 980 990 1000
PLGNEQNDFF PSVTVLVRGM SGRLAWAQQL CLLPRGAKAN QKLFVPEPRP
1010 1020 1030 1040 1050
VPKNDVGFKY SVKHRPFPEE VDKIPFVKAD LSIPDLHEIV TEELEERHEK
1060 1070 1080 1090 1100
LRSGMAQQIA YEMHLEQQSE GELQKRSFPD PVTDCKPPPP AQEFQTARLF
1110 1120 1130 1140 1150
LSHFGFLSLE ALKEPANSRL PPHLIALDST IPGFFDDIGY LDLLPCRPFD
1160 1170 1180 1190 1200
TVFIFYMKPG QKTNQEILKN VESSRNVQPH FLEFLLSLGW SVDVGKHPGW
1210 1220 1230 1240 1250
TGHVSTSWSI NSCDDGEGSE PDEITSSEDV GASIFNGQKK VLYYADALTE
1260 1270 1280 1290 1300
IAFVVPSPVE SLTDSLESNI SDQDSDSNMD LMPGILKQPP LTLELVPNHT
1310 1320 1330 1340 1350
DSLNSSQRLS PSSRMKKLPQ GRPVPPLGPE TRVSVVWVER YDDIENFPLS
1360 1370 1380 1390 1400
DLMTEISTGV ETTANSSTSL RSTTLEKEVP VIFIHPLNTG LFRIKIQGAT
1410 1420 1430 1440 1450
GKFNMVIPLV DGMIVSRRAL GFLVRQTVIN ICRRKRLESD SYSPPHVRRK
1460 1470 1480
QKITDIVNKY RNKQLEPEFY TALFQEVGLK NCSS
Length:1,484
Mass (Da):165,200
Last modified:March 15, 2004 - v2
Checksum:i24CD07B42542920C
GO
Isoform 2 (identifier: Q8BQZ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     473-490: ASFIQILLSYKSSIATQA → GKYAQKHVVIIPFLFLSF
     491-1484: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:490
Mass (Da):54,976
Checksum:i4254888EE6450071
GO

Sequence cautioni

The sequence BAC33137.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1477 – 14771V → A in BAC33137 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei473 – 49018ASFIQ…IATQA → GKYAQKHVVIIPFLFLSF in isoform 2. 1 PublicationVSP_009697Add
BLAST
Alternative sequencei491 – 1484994Missing in isoform 2. 1 PublicationVSP_009698Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046067 mRNA. Translation: BAC32589.1.
AK047718 mRNA. Translation: BAC33137.1. Different initiation.
AK122470 mRNA. Translation: BAC65752.1.
UniGeneiMm.398185.

Genome annotation databases

UCSCiuc008nqg.1. mouse. [Q8BQZ4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK046067 mRNA. Translation: BAC32589.1.
AK047718 mRNA. Translation: BAC33137.1. Different initiation.
AK122470 mRNA. Translation: BAC65752.1.
UniGeneiMm.398185.

3D structure databases

ProteinModelPortaliQ8BQZ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048430.

PTM databases

iPTMnetiQ8BQZ4.
PhosphoSiteiQ8BQZ4.

Proteomic databases

EPDiQ8BQZ4.
MaxQBiQ8BQZ4.
PaxDbiQ8BQZ4.
PRIDEiQ8BQZ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc008nqg.1. mouse. [Q8BQZ4-2]

Organism-specific databases

MGIiMGI:2444531. Ralgapb.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3652. Eukaryota.
ENOG411027C. LUCA.
HOGENOMiHOG000230877.
HOVERGENiHBG052199.
InParanoidiQ8BQZ4.
PhylomeDBiQ8BQZ4.

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.

Miscellaneous databases

PROiQ8BQZ4.
SOURCEiSearch...

Gene expression databases

CleanExiMM_B230339M05RIK.

Family and domain databases

InterProiIPR000331. Rap_GAP_dom.
[Graphical view]
SUPFAMiSSF111347. SSF111347. 3 hits.
PROSITEiPS50085. RAPGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1225-1484 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain and Corpus striatum.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 313-1484 (ISOFORM 1).
    Tissue: Brain.
  3. "Adipocytes contain a novel complex similar to the tuberous sclerosis complex."
    Gridley S., Chavez J.A., Lane W.S., Lienhard G.E.
    Cell. Signal. 18:1626-1632(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RALGAPA2, TISSUE SPECIFICITY.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-379; SER-421 AND THR-724, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiRLGPB_MOUSE
AccessioniPrimary (citable) accession number: Q8BQZ4
Secondary accession number(s): Q80TH5, Q8BQN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: March 15, 2004
Last modified: June 8, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.