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Protein

tRNA (uracil(54)-C(5))-methyltransferase homolog

Gene

Trmt2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Probable S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + uracil(54) in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil(54) in tRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei312 – 3121S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei362 – 3621S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei412 – 4121S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei440 – 4401NucleophilePROSITE-ProRule annotation
Active sitei486 – 4861Proton acceptorBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (uracil(54)-C(5))-methyltransferase homolog (EC:2.1.1.35)
Alternative name(s):
TRM2 homolog
Gene namesi
Name:Trmt2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2442530. Trmt2b.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493tRNA (uracil(54)-C(5))-methyltransferase homologPRO_0000311933Add
BLAST

Proteomic databases

PaxDbiQ8BQJ6.
PRIDEiQ8BQJ6.

PTM databases

PhosphoSiteiQ8BQJ6.

Expressioni

Gene expression databases

BgeeiQ8BQJ6.
ExpressionAtlasiQ8BQJ6. baseline and differential.
GenevisibleiQ8BQJ6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084820.

Structurei

3D structure databases

ProteinModelPortaliQ8BQJ6.
SMRiQ8BQJ6. Positions 300-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2187. Eukaryota.
COG2265. LUCA.
GeneTreeiENSGT00530000063723.
HOGENOMiHOG000232103.
HOVERGENiHBG108599.
InParanoidiQ8BQJ6.
KOiK15331.
OMAiHEGGYWR.
OrthoDBiEOG7327N9.
PhylomeDBiQ8BQJ6.
TreeFamiTF352239.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases.
IPR025823. tRNA_(uracil-5-)_MeTrfase_met.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8BQJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNPRLFLSR AGFFSKPGLL PWDTGRPPDL SQSLLGRAYK GSFTSVIAKK
60 70 80 90 100
HPSRQKYQKK QRHCRDDSWQ ERLADVVTPL WRLSYEAQLK VKFEAQKKLL
110 120 130 140 150
QSLESHLKVL HGVSDTVAAH QSEGLRCLLH PIIPSPTTTG YRNKSTFSVY
160 170 180 190 200
RSPDGNPKTV GYYLGTWKDG NVVCLPCNHL KNIPEKHSQV AQYYEVFLRQ
210 220 230 240 250
SSVEPCLLFH EGGHWRELVV RTNRQGHTMA IVTFHPQGLS EEEVCVQKVT
260 270 280 290 300
LKDFFTKGPG AICELTSLYF QESTMTRCSH QQSPYQLLFG EPHIFEDLLG
310 320 330 340 350
LKIRISPDAF FQINTAGAEM LYRIIGELSG VNSESLLLDI CCGTGVIGLS
360 370 380 390 400
VAQRASQVHG IELVEQAVED ARWTAAFNGV TNCEFHAGRA ETILPQLLKS
410 420 430 440 450
QKDEKLTVAV VNPARAGLHY RVVRAIRNCR TIHTLVFVSC KPHGESTRNF
460 470 480 490
IELCCPPNSA KQLLGDPFVL REAVPVDLFP HTPHCELVLL FTR
Length:493
Mass (Da):55,476
Last modified:March 1, 2003 - v1
Checksum:i81FF48C6A9F0314E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511R → K in BAC26233 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028999 mRNA. Translation: BAC26233.1.
AK049523 mRNA. Translation: BAC33793.1.
AL672215 Genomic DNA. Translation: CAM15919.1.
BC056937 mRNA. Translation: AAH56937.1.
CCDSiCCDS41121.1.
RefSeqiNP_001161466.1. NM_001167994.1.
NP_766128.2. NM_172540.2.
UniGeneiMm.289368.

Genome annotation databases

EnsembliENSMUST00000087541; ENSMUSP00000084820; ENSMUSG00000067369.
ENSMUST00000113252; ENSMUSP00000108878; ENSMUSG00000067369.
GeneIDi215201.
KEGGimmu:215201.
UCSCiuc009ufq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028999 mRNA. Translation: BAC26233.1.
AK049523 mRNA. Translation: BAC33793.1.
AL672215 Genomic DNA. Translation: CAM15919.1.
BC056937 mRNA. Translation: AAH56937.1.
CCDSiCCDS41121.1.
RefSeqiNP_001161466.1. NM_001167994.1.
NP_766128.2. NM_172540.2.
UniGeneiMm.289368.

3D structure databases

ProteinModelPortaliQ8BQJ6.
SMRiQ8BQJ6. Positions 300-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084820.

PTM databases

PhosphoSiteiQ8BQJ6.

Proteomic databases

PaxDbiQ8BQJ6.
PRIDEiQ8BQJ6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087541; ENSMUSP00000084820; ENSMUSG00000067369.
ENSMUST00000113252; ENSMUSP00000108878; ENSMUSG00000067369.
GeneIDi215201.
KEGGimmu:215201.
UCSCiuc009ufq.2. mouse.

Organism-specific databases

CTDi79979.
MGIiMGI:2442530. Trmt2b.

Phylogenomic databases

eggNOGiKOG2187. Eukaryota.
COG2265. LUCA.
GeneTreeiENSGT00530000063723.
HOGENOMiHOG000232103.
HOVERGENiHBG108599.
InParanoidiQ8BQJ6.
KOiK15331.
OMAiHEGGYWR.
OrthoDBiEOG7327N9.
PhylomeDBiQ8BQJ6.
TreeFamiTF352239.

Miscellaneous databases

NextBioi374634.
PROiQ8BQJ6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BQJ6.
ExpressionAtlasiQ8BQJ6. baseline and differential.
GenevisibleiQ8BQJ6. MM.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025714. Methyltranfer_dom.
IPR029063. SAM-dependent_MTases.
IPR025823. tRNA_(uracil-5-)_MeTrfase_met.
IPR010280. U5_MeTrfase_fam.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
PROSITEiPS51687. SAM_MT_RNA_M5U. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiTRM2_MOUSE
AccessioniPrimary (citable) accession number: Q8BQJ6
Secondary accession number(s): Q8CE55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: March 1, 2003
Last modified: November 11, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.