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Protein

tRNA (uracil(54)-C(5))-methyltransferase homolog

Gene

Trmt2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Probable S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine at position 54 (m5U54) in all tRNA. May also have a role in tRNA stabilization or maturation (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + uracil(54) in tRNA = S-adenosyl-L-homocysteine + 5-methyluracil(54) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei312S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei362S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei412S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei440NucleophilePROSITE-ProRule annotation1
Active sitei486Proton acceptorBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (uracil(54)-C(5))-methyltransferase homolog (EC:2.1.1.35)
Alternative name(s):
TRM2 homolog
Gene namesi
Name:Trmt2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2442530 Trmt2b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003119331 – 493tRNA (uracil(54)-C(5))-methyltransferase homologAdd BLAST493

Proteomic databases

PaxDbiQ8BQJ6
PRIDEiQ8BQJ6

PTM databases

iPTMnetiQ8BQJ6
PhosphoSitePlusiQ8BQJ6

Expressioni

Gene expression databases

BgeeiENSMUSG00000067369
ExpressionAtlasiQ8BQJ6 baseline and differential
GenevisibleiQ8BQJ6 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084820

Structurei

3D structure databases

ProteinModelPortaliQ8BQJ6
SMRiQ8BQJ6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2187 Eukaryota
COG2265 LUCA
GeneTreeiENSGT00530000063723
HOGENOMiHOG000232103
HOVERGENiHBG108599
InParanoidiQ8BQJ6
KOiK15331
OMAiHEGGYWR
OrthoDBiEOG091G0A4J
PhylomeDBiQ8BQJ6
TreeFamiTF352239

Family and domain databases

InterProiView protein in InterPro
IPR025714 Methyltranfer_dom
IPR029063 SAM-dependent_MTases
IPR025823 tRNA_(uracil-5-)_MeTrfase_met
IPR010280 U5_MeTrfase_fam
PANTHERiPTHR11061 PTHR11061, 1 hit
PfamiView protein in Pfam
PF13847 Methyltransf_31, 1 hit
SUPFAMiSSF53335 SSF53335, 3 hits
PROSITEiView protein in PROSITE
PS51687 SAM_MT_RNA_M5U, 1 hit

Sequencei

Sequence statusi: Complete.

Q8BQJ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNPRLFLSR AGFFSKPGLL PWDTGRPPDL SQSLLGRAYK GSFTSVIAKK
60 70 80 90 100
HPSRQKYQKK QRHCRDDSWQ ERLADVVTPL WRLSYEAQLK VKFEAQKKLL
110 120 130 140 150
QSLESHLKVL HGVSDTVAAH QSEGLRCLLH PIIPSPTTTG YRNKSTFSVY
160 170 180 190 200
RSPDGNPKTV GYYLGTWKDG NVVCLPCNHL KNIPEKHSQV AQYYEVFLRQ
210 220 230 240 250
SSVEPCLLFH EGGHWRELVV RTNRQGHTMA IVTFHPQGLS EEEVCVQKVT
260 270 280 290 300
LKDFFTKGPG AICELTSLYF QESTMTRCSH QQSPYQLLFG EPHIFEDLLG
310 320 330 340 350
LKIRISPDAF FQINTAGAEM LYRIIGELSG VNSESLLLDI CCGTGVIGLS
360 370 380 390 400
VAQRASQVHG IELVEQAVED ARWTAAFNGV TNCEFHAGRA ETILPQLLKS
410 420 430 440 450
QKDEKLTVAV VNPARAGLHY RVVRAIRNCR TIHTLVFVSC KPHGESTRNF
460 470 480 490
IELCCPPNSA KQLLGDPFVL REAVPVDLFP HTPHCELVLL FTR
Length:493
Mass (Da):55,476
Last modified:March 1, 2003 - v1
Checksum:i81FF48C6A9F0314E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti151R → K in BAC26233 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028999 mRNA Translation: BAC26233.1
AK049523 mRNA Translation: BAC33793.1
AL672215 Genomic DNA No translation available.
BC056937 mRNA Translation: AAH56937.1
CCDSiCCDS41121.1
RefSeqiNP_001161466.1, NM_001167994.1
NP_766128.2, NM_172540.2
UniGeneiMm.289368

Genome annotation databases

EnsembliENSMUST00000087541; ENSMUSP00000084820; ENSMUSG00000067369
ENSMUST00000113252; ENSMUSP00000108878; ENSMUSG00000067369
GeneIDi215201
KEGGimmu:215201
UCSCiuc009ufq.2 mouse

Similar proteinsi

Entry informationi

Entry nameiTRM2_MOUSE
AccessioniPrimary (citable) accession number: Q8BQJ6
Secondary accession number(s): Q8CE55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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