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Protein

Engulfment and cell motility protein 1

Gene

Elmo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
  • actin filament-based process Source: MGI
  • apoptotic process Source: UniProtKB-KW
  • cell migration Source: InterPro
  • cell motility Source: UniProtKB
  • phagocytosis Source: MGI
  • phagocytosis, engulfment Source: UniProtKB
  • Rac protein signal transduction Source: UniProtKB

Keywordsi

Biological processApoptosis, Phagocytosis

Enzyme and pathway databases

ReactomeiR-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-8849471 PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases

Names & Taxonomyi

Protein namesi
Recommended name:
Engulfment and cell motility protein 1
Alternative name(s):
Protein ced-12 homolog
Gene namesi
Name:Elmo1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:2153044 Elmo1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001537131 – 727Engulfment and cell motility protein 1Add BLAST727

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18Phosphotyrosine; by HCKBy similarity1
Modified residuei100N6-acetyllysineBy similarity1
Modified residuei105N6-acetyllysineBy similarity1
Modified residuei216Phosphotyrosine; by HCKBy similarity1
Modified residuei344PhosphoserineCombined sources1
Modified residuei395Phosphotyrosine; by HCKBy similarity1
Modified residuei511Phosphotyrosine; by HCKBy similarity1
Modified residuei720Phosphotyrosine; by HCKBy similarity1

Post-translational modificationi

Phosphorylated by HCK.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BPU7
PaxDbiQ8BPU7
PeptideAtlasiQ8BPU7
PRIDEiQ8BPU7

PTM databases

iPTMnetiQ8BPU7
PhosphoSitePlusiQ8BPU7

Expressioni

Gene expression databases

BgeeiENSMUSG00000041112
GenevisibleiQ8BPU7 MM

Interactioni

Subunit structurei

Interacts directly with the SH3-domain of DOCK1 via its SH3-binding site. Probably forms a heterotrimeric complex with DOCK1 and RAC1 (By similarity). Interacts with PLEKHG6. Interacts with HCK (via SH3 domain) (By similarity). Interacts with ADGRB1 (PubMed:17960134). Interacts with ADGRB3 (By similarity).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dock1Q8BUR413EBI-644162,EBI-646023

GO - Molecular functioni

Protein-protein interaction databases

BioGridi228288, 1 interactor
CORUMiQ8BPU7
DIPiDIP-41244N
IntActiQ8BPU7, 5 interactors
MINTiQ8BPU7
STRINGi10090.ENSMUSP00000072334

Structurei

3D structure databases

ProteinModelPortaliQ8BPU7
SMRiQ8BPU7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini319 – 492ELMOPROSITE-ProRule annotationAdd BLAST174
Domaini555 – 676PHAdd BLAST122

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi707 – 714SH3-binding8

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG2999 Eukaryota
ENOG410XPKN LUCA
GeneTreeiENSGT00390000014155
HOGENOMiHOG000252986
HOVERGENiHBG051463
InParanoidiQ8BPU7
KOiK12366
OMAiTHDHPFE
OrthoDBiEOG091G03JJ
PhylomeDBiQ8BPU7
TreeFamiTF312966

Family and domain databases

Gene3Di1.25.10.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR024574 DUF3361
IPR030715 ELMO1
IPR006816 ELMO_dom
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
PANTHERiPTHR12771:SF23 PTHR12771:SF23, 1 hit
PfamiView protein in Pfam
PF11841 DUF3361, 1 hit
PF04727 ELMO_CED12, 1 hit
PF16457 PH_12, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51335 ELMO, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BPU7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPPSDIVKV AIEWPGAYPK LMEIDQKKPL SAIIKEVCDG WSLANHEYFA
60 70 80 90 100
LQHADSSNFY ITEKNRNEIK NGTILRLTTS PAQNAQQLHE RIQSSSMDAK
110 120 130 140 150
LEALKDLASL SRDVTFAQEF INLDGISLLT QMVESGTERY QKLQKIMKPC
160 170 180 190 200
FGDMLSFTLT AFVELMDHGI VSWDTFSVAF IKKIASFVNK SAIDISILQR
210 220 230 240 250
SLAILESMVL NSHDLYQKVA QEITIGQLIP HLQGTDQEIQ TYTIAVINAL
260 270 280 290 300
FLKAPDERRQ EMANILAQKQ LRYIILTHVI RAQRAINNEM AHQLYVLQVL
310 320 330 340 350
TFNLLEDRMM TKMDPQDQAQ RDIIFELRRI AFDAESEPNN SSGSMEKRKS
360 370 380 390 400
MYTRDYKKLG FINHVNPAMD FTQTPPGMLA LDNMLYFAKH HQDAYIRIVL
410 420 430 440 450
ENSSREDKHE CPFGRSSIEL TKMLCEILKV GELPSETCND FHPMFFTHDR
460 470 480 490 500
SFEEFFCICI QLLNKTWKEM RATSEDFNKV MQVVKEQVMR ALTTKPSSLD
510 520 530 540 550
QFKSKLQNLS YTEILKIRQS ERMNQEDFQS RPILELKEKI QPEILELIKQ
560 570 580 590 600
QRLNRLVEGT CFRKLNARRR QDKFWYCRLS PNHKVLHYGD LEESPQGEVP
610 620 630 640 650
HDSLQDKLPV ADIKAVVTGK DCPHMKEKGA LKQNKEVLEL AFSILYDSNC
660 670 680 690 700
QLNFIAPDKH EYCIWTDGLN ALLGKDMMSD LTRNDLDTLL SMEIKLRLLD
710 720
LENIQIPDAP PPIPKEPSNY DFVYDCN
Length:727
Mass (Da):83,936
Last modified:May 16, 2003 - v2
Checksum:i21B1BB9195504D28
GO
Isoform 2 (identifier: Q8BPU7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-480: Missing.

Note: No experimental confirmation available.
Show »
Length:247
Mass (Da):28,742
Checksum:iD02BCEC8D8A4383F
GO
Isoform 3 (identifier: Q8BPU7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     398-420: IVLENSSREDKHECPFGRSSIEL → VSMLASLRYCQCRMEFCFPTYAQ
     421-727: Missing.

Note: No experimental confirmation available.
Show »
Length:420
Mass (Da):48,152
Checksum:i46C679F33F7C703F
GO
Isoform 4 (identifier: Q8BPU7-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-480: Missing.
     636-642: EVLELAF → VWFSKSL
     643-727: Missing.

Note: No experimental confirmation available.
Show »
Length:162
Mass (Da):19,044
Checksum:i260CB9B8C7353874
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9K → E in BAC25925 (PubMed:16141072).Curated1
Sequence conflicti315P → H in BAC25925 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0074811 – 480Missing in isoform 2 and isoform 4. 2 PublicationsAdd BLAST480
Alternative sequenceiVSP_007482398 – 420IVLEN…SSIEL → VSMLASLRYCQCRMEFCFPT YAQ in isoform 3. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_007483421 – 727Missing in isoform 3. 1 PublicationAdd BLAST307
Alternative sequenceiVSP_007484636 – 642EVLELAF → VWFSKSL in isoform 4. 1 Publication7
Alternative sequenceiVSP_007485643 – 727Missing in isoform 4. 1 PublicationAdd BLAST85

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF398883 mRNA Translation: AAL14464.1
AK028389 mRNA Translation: BAC25925.1
AK053306 mRNA Translation: BAC35336.1
BC024727 mRNA Translation: AAH24727.1
BC031782 mRNA Translation: AAH31782.1
CCDSiCCDS26265.1 [Q8BPU7-1]
RefSeqiNP_525027.1, NM_080288.2 [Q8BPU7-1]
NP_932761.1, NM_198093.3 [Q8BPU7-2]
XP_006516610.1, XM_006516547.3 [Q8BPU7-1]
UniGeneiMm.342392

Genome annotation databases

EnsembliENSMUST00000072519; ENSMUSP00000072334; ENSMUSG00000041112 [Q8BPU7-1]
ENSMUST00000180626; ENSMUSP00000152595; ENSMUSG00000041112 [Q8BPU7-3]
GeneIDi140580
KEGGimmu:140580
UCSCiuc007ppn.3 mouse [Q8BPU7-1]
uc007ppo.1 mouse [Q8BPU7-3]
uc007ppq.2 mouse [Q8BPU7-4]
uc007ppr.3 mouse [Q8BPU7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiELMO1_MOUSE
AccessioniPrimary (citable) accession number: Q8BPU7
Secondary accession number(s): Q8BSY9, Q8K2C5, Q91ZU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 16, 2003
Last modified: March 28, 2018
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health