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Protein

Small G protein signaling modulator 1

Gene

Sgsm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with numerous Rab family members, functioning as Rab effector for some, and as GTPase activator for others. Promotes GTP hydrolysis by RAB34 and RAB36. Probably functions as GTPase effector with RAB9A and RAB9B; does not stimulate GTP hydrolysis with RAB9A and RAB9B.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Names & Taxonomyi

Protein namesi
Recommended name:
Small G protein signaling modulator 1
Alternative name(s):
RUN and TBC1 domain-containing protein 2
Gene namesi
Name:Sgsm1
Synonyms:Rutbc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:107320. Sgsm1.

Subcellular locationi

  • Golgi apparatustrans-Golgi network 1 Publication
  • Cytoplasm 1 Publication
  • Cytoplasmic vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication

  • Note: Recruited to cytoplasmic vesicle membranes via its interaction with Rab family members, such as RAB9A.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi256 – 2561L → A: Abolishes interaction with RAB9A. 1 Publication
Mutagenesisi260 – 2601K → A: Abolishes interaction with RAB9A. 1 Publication
Mutagenesisi261 – 2611N → A: Abolishes interaction with RAB9A. 1 Publication
Mutagenesisi262 – 2621N → A: Mildly impaired interaction with RAB9A. 1 Publication
Mutagenesisi277 – 2771Y → A: Abolishes interaction with RAB9A. 1 Publication
Mutagenesisi294 – 2941N → A: Abolishes interaction with RAB9A. 1 Publication
Mutagenesisi297 – 2971M → A: Abolishes interaction with RAB9A. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10931093Small G protein signaling modulator 1PRO_0000284834Add
BLAST

Proteomic databases

MaxQBiQ8BPQ7.
PaxDbiQ8BPQ7.
PeptideAtlasiQ8BPQ7.
PRIDEiQ8BPQ7.

PTM databases

iPTMnetiQ8BPQ7.
PhosphoSiteiQ8BPQ7.

Expressioni

Tissue specificityi

Expressed only in brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000042216.
CleanExiMM_SGSM1.
ExpressionAtlasiQ8BPQ7. baseline and differential.
GenevisibleiQ8BPQ7. MM.

Interactioni

Subunit structurei

Interacts with RAB9A (GTP-bound form) and RAB9B (PubMed:25220469). Interacts with RAB3A, RAB4A, RAB5A, RAB8A, RAB11A, RAP1A, RAP1B, RAP2A and RAP2B. No interaction with RAB27A (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-61070N.
STRINGi10090.ENSMUSP00000046544.

Structurei

Secondary structure

1
1093
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi255 – 26511Combined sources
Beta strandi267 – 2715Combined sources
Beta strandi274 – 28512Combined sources
Beta strandi287 – 2937Combined sources
Helixi294 – 2963Combined sources
Turni297 – 2993Combined sources
Turni303 – 3053Combined sources
Helixi306 – 3083Combined sources
Helixi310 – 3134Combined sources
Beta strandi316 – 3194Combined sources
Helixi320 – 3223Combined sources
Beta strandi323 – 3297Combined sources
Beta strandi337 – 3426Combined sources
Beta strandi355 – 3573Combined sources
Helixi358 – 37013Combined sources
Turni371 – 3733Combined sources
Beta strandi375 – 3784Combined sources
Helixi384 – 3863Combined sources
Beta strandi416 – 4227Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QXAX-ray2.30B254-425[»]
ProteinModelPortaliQ8BPQ7.
SMRiQ8BPQ7. Positions 254-424, 538-581, 882-1063.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 190155RUNPROSITE-ProRule annotationAdd
BLAST
Domaini562 – 1026465Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni256 – 29742Important for interaction with RAB9A and RAB9B1 PublicationAdd
BLAST
Regioni301 – 35050Required for interaction with RAP family membersBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the RUTBC family.Curated
Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation
Contains 1 RUN domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1648. Eukaryota.
COG5210. LUCA.
GeneTreeiENSGT00550000074386.
HOGENOMiHOG000007057.
HOVERGENiHBG108483.
InParanoidiQ8BPQ7.
PhylomeDBiQ8BPQ7.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
IPR004012. Run_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
PF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00593. RUN. 1 hit.
SM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 3 hits.
PROSITEiPS50826. RUN. 1 hit.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BPQ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASVPAEAET RQRLLRTVKK EVKQIMEEAV TRKFVHEDSS HIISFCAAVE
60 70 80 90 100
ACVLHGLRRR AAGFLRSNKI AALFMKVGKG FPPAEELSRK VQELEQLIES
110 120 130 140 150
ARNQIQGLQE NVRKLPKLPN LSPLAIKHLW IRTALFGRVL DKIVHYLVEN
160 170 180 190 200
SSKYYEKEAL LMDPVDGPIL ASLLVGPCAL EYTKMKTADH FWTDPSADEL
210 220 230 240 250
VQRHRIHSSH LRQDSPTKRP ALCIQKRHSS GSMDDRPSIS ARDYVESLHQ
260 270 280 290 300
DSRATLLYGK NNVLVQPRDD MEAVPGYLSL HQTADVMTLK WTPNQLMNGS
310 320 330 340 350
VGDLDYEKSV YWDYAVTIRL EEIVYLHCHQ QVDSGGTVVL VSQDGIQRPP
360 370 380 390 400
FRFPKGGHLL QFLSCLENGL LPHGQLDPPL WSQRGKGKVF PKLRKRSPQG
410 420 430 440 450
SSESTSSDKE DDEATDYVFR IIYPGTQSEF VPQDLMDVSM NNLPPLWQPS
460 470 480 490 500
PRKSSCSSCS QSGSADGGST NGCNHERAPL KLLCDNMKYQ ILSRAFYGWL
510 520 530 540 550
AYCRHLSTVR THLSALVNHM IVSPDLPCDA GQGLTASIWE KYIQDSTTYP
560 570 580 590 600
EQELLRLIYY GGVQPEIRRA VWPFLLGHYQ FGMTEMERKE VDEQIHACYA
610 620 630 640 650
QTMSEWLGCE AIVRQRERES HAAALAKCSS GASLDSHLHR MLHRDSTISN
660 670 680 690 700
ESSQSCSSGR QNLRLQSDSS SSTQVFESVD EVEQTEAEGR SEEKHPKIPN
710 720 730 740 750
GNPANGTCSP DSGHPSSHNF SSGLSEHSEP SLSTEDSVLD AQRSLPAVFR
760 770 780 790 800
PGDSSVEDGQ SSEATTSRDE APREELAVQD SLESDLLANE SLEEFMSIPG
810 820 830 840 850
SLDVALPEKD GAVMDGWPGE ADKPSRADSE DNLSEEPEME SLFPALASLA
860 870 880 890 900
VTSSANNEAS PVSSSGVTYS PELLDLYTVN LHRIEKDVQR CDRSYWYFTA
910 920 930 940 950
ANLEKLRNIM CSYIWQHIEI GYVQGMCDLL APLLVILDDE ALAFSCFTEL
960 970 980 990 1000
MKRMNQNFPH GGAMDTHFAN MRSLIQILDS ELFELMHQNG DYTHFYFCYR
1010 1020 1030 1040 1050
WFLLDFKREL VYDDVFSVWE TIWAAKHVSS AHYVLFIALA LVEVYRDIIL
1060 1070 1080 1090
ENNMDFTDII KFFNEMAERH NAKQILQLAR DLVHKVQILI ENK
Length:1,093
Mass (Da):123,196
Last modified:April 17, 2007 - v2
Checksum:i947E0CDB3220A6FA
GO
Isoform 2 (identifier: Q8BPQ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-287: Missing.
     288-308: TLKWTPNQLMNGSVGDLDYEK → MLGQVGLGDLWLPTVSLTSPS

Note: No experimental confirmation available.
Show »
Length:806
Mass (Da):90,498
Checksum:i70B7E2A118BB6A4A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti321 – 3211E → V in BAE39683 (PubMed:16141072).Curated
Sequence conflicti550 – 5501P → T in BAC35428 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 287287Missing in isoform 2. 1 PublicationVSP_024669Add
BLAST
Alternative sequencei288 – 30821TLKWT…LDYEK → MLGQVGLGDLWLPTVSLTSP S in isoform 2. 1 PublicationVSP_024670Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053555 mRNA. Translation: BAC35428.1.
AK167632 mRNA. Translation: BAE39683.1.
CCDSiCCDS39218.1. [Q8BPQ7-1]
RefSeqiNP_001156437.1. NM_001162965.1.
NP_001296457.1. NM_001309528.1. [Q8BPQ7-2]
NP_766306.2. NM_172718.3.
UniGeneiMm.200203.

Genome annotation databases

EnsembliENSMUST00000057209; ENSMUSP00000084106; ENSMUSG00000042216. [Q8BPQ7-2]
GeneIDi52850.
KEGGimmu:52850.
UCSCiuc008yuc.2. mouse. [Q8BPQ7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053555 mRNA. Translation: BAC35428.1.
AK167632 mRNA. Translation: BAE39683.1.
CCDSiCCDS39218.1. [Q8BPQ7-1]
RefSeqiNP_001156437.1. NM_001162965.1.
NP_001296457.1. NM_001309528.1. [Q8BPQ7-2]
NP_766306.2. NM_172718.3.
UniGeneiMm.200203.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4QXAX-ray2.30B254-425[»]
ProteinModelPortaliQ8BPQ7.
SMRiQ8BPQ7. Positions 254-424, 538-581, 882-1063.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61070N.
STRINGi10090.ENSMUSP00000046544.

PTM databases

iPTMnetiQ8BPQ7.
PhosphoSiteiQ8BPQ7.

Proteomic databases

MaxQBiQ8BPQ7.
PaxDbiQ8BPQ7.
PeptideAtlasiQ8BPQ7.
PRIDEiQ8BPQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057209; ENSMUSP00000084106; ENSMUSG00000042216. [Q8BPQ7-2]
GeneIDi52850.
KEGGimmu:52850.
UCSCiuc008yuc.2. mouse. [Q8BPQ7-2]

Organism-specific databases

CTDi129049.
MGIiMGI:107320. Sgsm1.

Phylogenomic databases

eggNOGiKOG1648. Eukaryota.
COG5210. LUCA.
GeneTreeiENSGT00550000074386.
HOGENOMiHOG000007057.
HOVERGENiHBG108483.
InParanoidiQ8BPQ7.
PhylomeDBiQ8BPQ7.

Miscellaneous databases

PROiQ8BPQ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042216.
CleanExiMM_SGSM1.
ExpressionAtlasiQ8BPQ7. baseline and differential.
GenevisibleiQ8BPQ7. MM.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
IPR004012. Run_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
PF02759. RUN. 1 hit.
[Graphical view]
SMARTiSM00593. RUN. 1 hit.
SM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 3 hits.
PROSITEiPS50826. RUN. 1 hit.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSGSM1_MOUSE
AccessioniPrimary (citable) accession number: Q8BPQ7
Secondary accession number(s): Q3TJ12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: April 17, 2007
Last modified: September 7, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.