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Protein

DmX-like protein 2

Gene

Dmxl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May serve as a scaffold protein for MADD and RAB3GA on synaptic vesicles of neuronal and endocrine homeostatic processes (By similarity). Plays a role in the brain as a key controller of neuronal and endocrine homeostatic processes (PubMed:25248098).By similarity1 Publication

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
DmX-like protein 2
Alternative name(s):
Rabconnectin-3
Gene namesi
Name:Dmxl2
Synonyms:Kiaa0856
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2444630. Dmxl2.

Subcellular locationi

  • Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane By similarity; Peripheral membrane protein By similarity

  • Note: The external layer of the inferior boundary for the hypothalamus part of the human brain (the so called median eminence (ME)) displayed a punctate pattern of expression; expression also observed in the cell bodies lining the third ventricle, in the long processes extending from these cell bodies toward the external layer of the ME, in small clear vesicles, and in large dense core vesicles.1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasmic vesicle, Membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Conditional heterozygous deletion of the gene in neurons causes delayed puberty as well as very low fertility; the reproductive phenotype is associated with a reduced number of GnRH neurons in the hypothalamus of adult mice. Animals with a homozygous deletion of the gene die during embryonic development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 30323032DmX-like protein 2PRO_0000223325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei326 – 3261PhosphoserineCombined sources
Modified residuei473 – 4731PhosphoserineBy similarity
Modified residuei587 – 5871PhosphoserineCombined sources
Modified residuei945 – 9451PhosphoserineCombined sources
Modified residuei946 – 9461PhosphoserineCombined sources
Modified residuei1141 – 11411PhosphoserineCombined sources
Modified residuei1144 – 11441PhosphoserineCombined sources
Modified residuei1152 – 11521PhosphoserineBy similarity
Modified residuei1288 – 12881PhosphoserineCombined sources
Modified residuei1399 – 13991PhosphoserineBy similarity
Modified residuei1416 – 14161PhosphothreonineCombined sources
Modified residuei1856 – 18561PhosphoserineCombined sources
Modified residuei2017 – 20171PhosphothreonineCombined sources
Modified residuei2394 – 23941PhosphoserineCombined sources
Modified residuei2636 – 26361PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BPN8.
PaxDbiQ8BPN8.
PeptideAtlasiQ8BPN8.
PRIDEiQ8BPN8.

PTM databases

iPTMnetiQ8BPN8.
PhosphoSiteiQ8BPN8.
SwissPalmiQ8BPN8.

Expressioni

Tissue specificityi

Expressed in the brain and pituitary gland. Detected in the hippocampus, dentate gyrus, hypothalamus, pyriform cortex and the granular and molecular layers of the cerebellum of adult animals. In the hypothalamus, expression is observed in the arcuate nucleus, the ME, the organum vasculosum of the lamina terminalis, and the subfornical organ, the subcommissural organ, and the suprachiasmatic nucleus. Both tanycytes and hypothalamic neurosecretory neurons express the protein. Expressed in insulin-secreting cells of the islets of Langerhans in the pancreas.1 Publication

Gene expression databases

BgeeiENSMUSG00000041268.
CleanExiMM_DMXL2.
ExpressionAtlasiQ8BPN8. baseline and differential.

Interactioni

Subunit structurei

Interacts with MADD and RAB3GAP.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BPN8. 13 interactions.
MINTiMINT-4115211.
STRINGi10090.ENSMUSP00000113705.

Structurei

3D structure databases

ProteinModelPortaliQ8BPN8.
SMRiQ8BPN8. Positions 172-199, 1249-1274, 1712-1751.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati108 – 14538WD 1Add
BLAST
Repeati167 – 20741WD 2Add
BLAST
Repeati230 – 27849WD 3Add
BLAST
Repeati492 – 53241WD 4Add
BLAST
Repeati594 – 63340WD 5Add
BLAST
Repeati750 – 80253WD 6Add
BLAST
Repeati879 – 92143WD 7Add
BLAST
Repeati1001 – 103838WD 8Add
BLAST
Repeati1164 – 120542WD 9Add
BLAST
Repeati1245 – 128541WD 10Add
BLAST
Repeati2757 – 279640WD 11Add
BLAST
Repeati2800 – 283940WD 12Add
BLAST
Repeati2846 – 288843WD 13Add
BLAST
Repeati2894 – 293340WD 14Add
BLAST
Repeati2936 – 297540WD 15Add
BLAST
Repeati2988 – 302639WD 16Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili2117 – 214630Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 16 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1064. Eukaryota.
ENOG410XPUM. LUCA.
GeneTreeiENSGT00390000000096.
HOGENOMiHOG000018348.
HOVERGENiHBG079593.
InParanoidiQ8BPN8.
PhylomeDBiQ8BPN8.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR022033. Rav1p_C.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12234. Rav1p_C. 2 hits.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BPN8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHLHQVLTGA VNPGDNCYSV GSVGDVPFTA YGSGCDIVIL ASDFECVQII
60 70 80 90 100
PGAKHGNIQV SCVECSNQHG RVAASYGNAV CIFEPLGVNS HKRNSQLKCQ
110 120 130 140 150
WLKTGQFFLS SVTYNLAWDP QDNRLLTATD SIQLWAPPGG DILEEEEDVD
160 170 180 190 200
NRAPPVLNDW KCIWQCKTSV SVHLMEWSPD GEYFATAGKD DCLLKVWYPM
210 220 230 240 250
TGWKSSIIPQ DPHEVKRRRA STQFSFVYLA HPRAVTGFSW RKTSKYMPRG
260 270 280 290 300
SVCNVLLTSC HDGVCRLWAE TLLPEDCLLG EQICETTTSS VASNLSSAGK
310 320 330 340 350
HKDRIQHALE TIHHLKNLRK GQRRSSVLVT HAELMPDKTA THEVHRHISH
360 370 380 390 400
HANALCHFHI AASINPTTDI PNVLVGTAFN IDDINGGFVV HWLNNKEFHF
410 420 430 440 450
TSSTEIFMHQ LRKLSEKQLD HESDDADRED EERSQDERER GLRMKLDHEL
460 470 480 490 500
SLDRESEAGT GSSEHEDGER EGSPRTHPRP SISMPLPTVL LDRKIETLLT
510 520 530 540 550
EWNKNPDMLF TIHPVDGTFL VWHVKYLDEY NPGIFRQVQV SFSSRIPVAF
560 570 580 590 600
PSGDANSLSK NIMMYACVNA TKDSYNPSQQ EMMSVDSPHG SQLHSPSHST
610 620 630 640 650
DMNILAPTVM MVSKHIDGSL NQWAVTFADK SAFTTVLTVS HKFRYCGHRF
660 670 680 690 700
HLNDLACHSV LPLLLTSSHH NALLTPESDC QWDSDSKVNR LIDPVKHTKA
710 720 730 740 750
SSKQPLRNAA TRTFHDPNAI YSELILWRVD PIGPLSYTGG VSELARINSL
760 770 780 790 800
HTSAFSNVAW LPTLIPSYCL GTYCNSASAC FVASDGKNLR LYQAVVDARK
810 820 830 840 850
LLDELSDPEA SKLIGEVFNI VSQQSTARPG CIIELDAITD QCGSNTQLLH
860 870 880 890 900
VFQEDFIIGY KPHKEDMEKK EKESEIFFQP SQGYRPPPFS EKFFLVVIEK
910 920 930 940 950
DGNNNSILHM WHLHLKSVQA CLAKAAEGIS SDSLLSVPGQ KNLDSSPETS
960 970 980 990 1000
SSMSSVPHSS SIANLQTASK LILSSRLVYS QPLDLPEAVE VIRATPSAGH
1010 1020 1030 1040 1050
LSSSSIYPVC LAPYLVVTTC SDNKVRFWKC CMETNSLGNT SDESETYHWR
1060 1070 1080 1090 1100
RWPLMNDEGE DNSSTVSIVG RPVAVSCSYT GRLAVAYKQP IHHNGFISKE
1110 1120 1130 1140 1150
FSMHVCIFEC ESTGGSEWVL EQTIHLDDLV KVGSVLDSRV SVDSNLFVYS
1160 1170 1180 1190 1200
KSDAFLSKDR YLIPNIKHLV HLDWVSKEDG SHILTVGVGA NIFMYGRLSG
1210 1220 1230 1240 1250
IVSDQTNSKD GVAVITLPLG GSIKQGVKSR WVLLRSIDLV SSVDGTPSLP
1260 1270 1280 1290 1300
VSLSWVRDGI LVVGMDCEMH VYAQWKHSVK FGNVDADSPV EETIQDHSAL
1310 1320 1330 1340 1350
KSSMLARKSI VEGAAIPDDV FCSPTVVQDG GLFEAAHALS PTLPQYHPTQ
1360 1370 1380 1390 1400
LLELMDLGKV RRAKAILSHL VKCIAGEVAI VRDPDAGEGT KRHLSRTISV
1410 1420 1430 1440 1450
SGSTAKDTVT IGKDGTRDYT EIDSIPPLPL HALLAADQDT SYRISEDSTK
1460 1470 1480 1490 1500
KPQSYEDHIE SQSEDQYSEL FQVQEITTDD IDLEPEKREN KSKVINLSQY
1510 1520 1530 1540 1550
GPACFGQEHA RVLSSHLMHS SLPGLTRLEQ MFLVALADTV ATTSTELDEN
1560 1570 1580 1590 1600
RDKNYSGRDT LDECGLRYLL AMRLHTCLLT SLPPLYRVQL LHQGVSTCHF
1610 1620 1630 1640 1650
AWAFHSEAEE ELINMIPAIQ RGDPQWSELR AMGIGWWVRN VNTLRRCIEK
1660 1670 1680 1690 1700
VAKAAFQRNN EALDAALFYL SMKKKAVVWG LFRSQHDEKM TTFFSHNFNE
1710 1720 1730 1740 1750
DRWRKAALKN AFSLLGKQRF EQSAAFFLLA GSLKDAIEVC LEKMEDIQLA
1760 1770 1780 1790 1800
MVIARLFESE FETSSTYISI LNQKILGCQK DGTGFDCKRL HPDPFLRSLA
1810 1820 1830 1840 1850
YWVVKDYTRA LDTLLEQTPK EDDEQQVIIK SCNPVVFSFY NYLRTHPLLI
1860 1870 1880 1890 1900
RRNLASPEGT LATLGLKTEK NIADKINLIE RKLFFTTANA HFKVGCPVLA
1910 1920 1930 1940 1950
LEVLSKIPKV TKISSLTAKK DQLDSVSGRM ENGPSESKPV SRSDGGSGAD
1960 1970 1980 1990 2000
WSAVTSSQFD WSQPMVTVDE EPLRLDWGDD HDGALEEDDG GGLVMKTTDA
2010 2020 2030 2040 2050
KKAGQEQSAS DPRALLTPQD EECADGDTEV DVIAEQLKFR ACLKILMTEL
2060 2070 2080 2090 2100
RTLATGYEVD GGKLRFQLYN WLEKEIAALH EICNHESVIK EYSSKAHSTV
2110 2120 2130 2140 2150
ETERLDQEEM VDKPDIGSYE RHQIERRRLQ AKREHAERRK LWLQKNQDLL
2160 2170 2180 2190 2200
RVFLSYCSLH GAQGGGLASV RMELKFLLQE SQQETTVKQL QSPLPLPTTL
2210 2220 2230 2240 2250
PLLSASIAST KTVIANPVLY LNNHIHDILY TIVQMKTPPH PSVEDVKVHT
2260 2270 2280 2290 2300
LHSLAASLSA SIYQALCDSH SYSQSEGNQF TGMAYQGLLL SDRRRLRTES
2310 2320 2330 2340 2350
IEEHATPNSA PAQWPGVSSL INLLSSAQDE DQPKLNVLLC EAVVAVYLSL
2360 2370 2380 2390 2400
LIHALATNSS NELFRLAAHP LNNRMWAAVF GGGVKLVVKP RRQSESIAAP
2410 2420 2430 2440 2450
PVASEDMDKH RRRFNMRMLV PGRPVKDATP PPVPAERPSY KEKFIPPELS
2460 2470 2480 2490 2500
MWDYFVAKPF LPLSDSGVIY DSDESVHSDD EEDDAFFSDT QIQEHQDPNS
2510 2520 2530 2540 2550
YSWALLHLTM VKLALHNIKN FFPIAGLEFS ELPVTSPLGI AVIKNLENWE
2560 2570 2580 2590 2600
QILQEKMDHF EGPPPNYVNT YPTDLSVGAG PAILRNKAML EPENTPFKSR
2610 2620 2630 2640 2650
DSSALPVKRL WHFLVKQEVL QETFIRYIFT KKRKQSEVEA DLGYPGGKAK
2660 2670 2680 2690 2700
VIHKESDMIM AFSINKANCN EIVLASTHDV QELDVTSLLA CQSYIWIGEE
2710 2720 2730 2740 2750
YDRESKSSDD IDYRGSTTTL YQPGAASHSS SQPHPPPSLP WLGSGQTSTG
2760 2770 2780 2790 2800
ATVLMKRNLH NVKRMTSHPV HQYYLTGAQD GSVRMFEWTR PQQLVCFRQA
2810 2820 2830 2840 2850
GNARVTRLYF NSQGNKCGVA DGEGFLSIWQ VNQTASNPKP YMSWQCHSKA
2860 2870 2880 2890 2900
TSDFAFITSS SLVATSGHSN DNRNVCLWDT LISPGNSLIH GFTCHDHGAT
2910 2920 2930 2940 2950
VLQYAPKQQL LISGGRKGYI CIFDIRQRQL IHTFQAHDSA IKALALDSCE
2960 2970 2980 2990 3000
EYFTTGSAEG NIKVWRLTGH GLIHSFKSEH AKQSIFRNIG AGVMQIAISQ
3010 3020 3030
DNRLFSCGAD GTLKTRVLPS AFNIPNRILD IL
Length:3,032
Mass (Da):338,209
Last modified:July 10, 2007 - v3
Checksum:i2FB99AE184E56207
GO
Isoform 2 (identifier: Q8BPN8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2272-2272: Y → YS
     2488-2498: Missing.

Show »
Length:3,022
Mass (Da):337,017
Checksum:i5AB22DC3395B76F8
GO
Isoform 3 (identifier: Q8BPN8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     923-933: AKAAEGISSDS → GKKLYCYFIQR
     934-3032: Missing.

Show »
Length:933
Mass (Da):104,736
Checksum:i9B421C4D30044A5F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti655 – 6551L → P in BAC35468 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei923 – 93311AKAAEGISSDS → GKKLYCYFIQR in isoform 3. 1 PublicationVSP_026594Add
BLAST
Alternative sequencei934 – 30322099Missing in isoform 3. 1 PublicationVSP_026595Add
BLAST
Alternative sequencei2272 – 22721Y → YS in isoform 2. 1 PublicationVSP_026596
Alternative sequencei2488 – 249811Missing in isoform 2. 1 PublicationVSP_026597Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032099 mRNA. Translation: BAC27698.1.
AK053672 mRNA. Translation: BAC35468.2.
AK164884 mRNA. Translation: BAE37952.1.
CT010507, AC161589 Genomic DNA. Translation: CAQ12128.1.
AK173043 Transcribed RNA. Translation: BAD32321.1.
RefSeqiXP_006511148.2. XM_006511085.2. [Q8BPN8-1]
UniGeneiMm.93636.

Genome annotation databases

EnsembliENSMUST00000118600; ENSMUSP00000113693; ENSMUSG00000041268. [Q8BPN8-1]
GeneIDi235380.
UCSCiuc009prb.1. mouse. [Q8BPN8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032099 mRNA. Translation: BAC27698.1.
AK053672 mRNA. Translation: BAC35468.2.
AK164884 mRNA. Translation: BAE37952.1.
CT010507, AC161589 Genomic DNA. Translation: CAQ12128.1.
AK173043 Transcribed RNA. Translation: BAD32321.1.
RefSeqiXP_006511148.2. XM_006511085.2. [Q8BPN8-1]
UniGeneiMm.93636.

3D structure databases

ProteinModelPortaliQ8BPN8.
SMRiQ8BPN8. Positions 172-199, 1249-1274, 1712-1751.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BPN8. 13 interactions.
MINTiMINT-4115211.
STRINGi10090.ENSMUSP00000113705.

PTM databases

iPTMnetiQ8BPN8.
PhosphoSiteiQ8BPN8.
SwissPalmiQ8BPN8.

Proteomic databases

MaxQBiQ8BPN8.
PaxDbiQ8BPN8.
PeptideAtlasiQ8BPN8.
PRIDEiQ8BPN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000118600; ENSMUSP00000113693; ENSMUSG00000041268. [Q8BPN8-1]
GeneIDi235380.
UCSCiuc009prb.1. mouse. [Q8BPN8-3]

Organism-specific databases

CTDi23312.
MGIiMGI:2444630. Dmxl2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1064. Eukaryota.
ENOG410XPUM. LUCA.
GeneTreeiENSGT00390000000096.
HOGENOMiHOG000018348.
HOVERGENiHBG079593.
InParanoidiQ8BPN8.
PhylomeDBiQ8BPN8.

Miscellaneous databases

PROiQ8BPN8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000041268.
CleanExiMM_DMXL2.
ExpressionAtlasiQ8BPN8. baseline and differential.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR022033. Rav1p_C.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12234. Rav1p_C. 2 hits.
PF00400. WD40. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 13 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDMXL2_MOUSE
AccessioniPrimary (citable) accession number: Q8BPN8
Secondary accession number(s): B0V2P4
, Q3TNY5, Q69ZX5, Q8CCU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 10, 2007
Last modified: September 7, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.