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Protein

Protein RIC-3

Gene

Ric3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes functional expression of homomeric alpha-7 and alpha-8 nicotinic acetylcholine receptors at the cell surface. May also promote functional expression of homomeric serotoninergic 5-HT3 receptors, and of heteromeric acetylcholine receptors alpha-3/beta-2, alpha-3/beta-4, alpha-4/beta-2 and alpha-4/beta-4.1 Publication

GO - Molecular functioni

  1. acetylcholine receptor binding Source: MGI
  2. protein binding involved in protein folding Source: MGI

GO - Biological processi

  1. cellular protein complex assembly Source: MGI
  2. positive regulation of cytosolic calcium ion concentration Source: MGI
  3. protein folding Source: GOC
  4. synaptic transmission, cholinergic Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RIC-3
Alternative name(s):
Resistant to inhibitor of cholinesterase 3
Gene namesi
Name:Ric3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2443887. Ric3.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 9564LumenalSequence AnalysisAdd
BLAST
Transmembranei96 – 11621HelicalSequence AnalysisAdd
BLAST
Topological domaini117 – 367251CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 31311 PublicationAdd
BLAST
Chaini32 – 367336Protein RIC-3PRO_0000302732Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011N6-acetyllysine; alternateBy similarity
Cross-linki201 – 201Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ8BPM6.
PRIDEiQ8BPM6.

PTM databases

PhosphoSiteiQ8BPM6.

Expressioni

Tissue specificityi

Expressed in brain, with highest levels in hippocampus, cerebellum and superior colliculus.1 Publication

Gene expression databases

BgeeiQ8BPM6.
ExpressionAtlasiQ8BPM6. baseline.
GenevestigatoriQ8BPM6.

Interactioni

Subunit structurei

Monomer and homodimer. Interacts with CHRNA7, CHRNA3, CHRNA4, CHRNB2, CHRNB4 and HTR3A (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056990.

Structurei

3D structure databases

ProteinModelPortaliQ8BPM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili138 – 169321 PublicationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi81 – 9414Poly-GlyAdd
BLAST

Domaini

The coiled-coil domain mediates transient homodimerization with other acetylcholine receptor-bound RIC3 molecules, promoting stepwise ACHR homomeric assembly at the membrane.

Sequence similaritiesi

Belongs to the ric-3 family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG46432.
GeneTreeiENSGT00440000034107.
HOGENOMiHOG000034648.
HOVERGENiHBG106009.
InParanoidiQ8BPM6.
OMAiHRKITNF.
OrthoDBiEOG7MKW7H.
PhylomeDBiQ8BPM6.
TreeFamiTF333291.

Family and domain databases

InterProiIPR026160. Ric3.
[Graphical view]
PANTHERiPTHR21723. PTHR21723. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BPM6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAYSTVQRVA LASGLVLAVS LLLPKAFLSR GKRPEPPPGP EGKLDRFPPM
60 70 80 90 100
MHHHSAPSDG QTPGARFQRS HLAEAFAKAK GAGGGAGGGG SGRGLMGQII
110 120 130 140 150
PIYGFGIFLY ILYILFKLSK GKTAEDRNCS TAPPGNAHRK ITNFELVQLQ
160 170 180 190 200
EKLKETEEAM EKLINRVGPN GESRAQAVTS DQEKRLLHQL REITRVMKEG
210 220 230 240 250
KFIDTSPEKE AEEAPYMEDW EGYPEETYPI YDLSDGIKRR QETILVDYPD
260 270 280 290 300
LKEPSAEEIA EQMGEIEEEG SERLSWDHLP TDPGAQKDNS VAPCDPKPES
310 320 330 340 350
CSCCVHEEED PAVLAENAGF SADGYSEQEE ATKENLPQDF TNEGLGVSTD
360
NAHVGGMLRK RNPQGFE
Length:367
Mass (Da):40,283
Last modified:September 11, 2007 - v2
Checksum:i688D772716BDE892
GO
Isoform 2 (identifier: Q8BPM6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-159: Missing.

Note: No experimental confirmation available.

Show »
Length:208
Mass (Da):23,347
Checksum:iD59EB1FB2925FC26
GO
Isoform 3 (identifier: Q8BPM6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     223-228: YPEETY → KMPLPC
     229-367: Missing.

Show »
Length:228
Mass (Da):24,909
Checksum:i5A7F826B78472F08
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 461R → Q in BAC38452 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 159159Missing in isoform 2. 1 PublicationVSP_027944Add
BLAST
Alternative sequencei223 – 2286YPEETY → KMPLPC in isoform 3. 1 PublicationVSP_027945
Alternative sequencei229 – 367139Missing in isoform 3. 1 PublicationVSP_027946Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053760 mRNA. Translation: BAC35510.1.
AK082275 mRNA. Translation: BAC38452.1.
AK149334 mRNA. Translation: BAE28817.1.
BC059258 mRNA. Translation: AAH59258.1.
CCDSiCCDS21733.1. [Q8BPM6-1]
RefSeqiNP_001033713.1. NM_001038624.1. [Q8BPM6-1]
NP_848895.2. NM_178780.3. [Q8BPM6-3]
XP_006508005.1. XM_006507942.1. [Q8BPM6-2]
XP_006508006.1. XM_006507943.1. [Q8BPM6-2]
UniGeneiMm.71007.

Genome annotation databases

EnsembliENSMUST00000055993; ENSMUSP00000056990; ENSMUSG00000048330. [Q8BPM6-1]
GeneIDi320360.
KEGGimmu:320360.
UCSCiuc009jdg.1. mouse. [Q8BPM6-1]
uc009jdi.1. mouse. [Q8BPM6-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053760 mRNA. Translation: BAC35510.1.
AK082275 mRNA. Translation: BAC38452.1.
AK149334 mRNA. Translation: BAE28817.1.
BC059258 mRNA. Translation: AAH59258.1.
CCDSiCCDS21733.1. [Q8BPM6-1]
RefSeqiNP_001033713.1. NM_001038624.1. [Q8BPM6-1]
NP_848895.2. NM_178780.3. [Q8BPM6-3]
XP_006508005.1. XM_006507942.1. [Q8BPM6-2]
XP_006508006.1. XM_006507943.1. [Q8BPM6-2]
UniGeneiMm.71007.

3D structure databases

ProteinModelPortaliQ8BPM6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056990.

PTM databases

PhosphoSiteiQ8BPM6.

Proteomic databases

MaxQBiQ8BPM6.
PRIDEiQ8BPM6.

Protocols and materials databases

DNASUi320360.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055993; ENSMUSP00000056990; ENSMUSG00000048330. [Q8BPM6-1]
GeneIDi320360.
KEGGimmu:320360.
UCSCiuc009jdg.1. mouse. [Q8BPM6-1]
uc009jdi.1. mouse. [Q8BPM6-3]

Organism-specific databases

CTDi79608.
MGIiMGI:2443887. Ric3.

Phylogenomic databases

eggNOGiNOG46432.
GeneTreeiENSGT00440000034107.
HOGENOMiHOG000034648.
HOVERGENiHBG106009.
InParanoidiQ8BPM6.
OMAiHRKITNF.
OrthoDBiEOG7MKW7H.
PhylomeDBiQ8BPM6.
TreeFamiTF333291.

Miscellaneous databases

NextBioi396576.
PROiQ8BPM6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BPM6.
ExpressionAtlasiQ8BPM6. baseline.
GenevestigatoriQ8BPM6.

Family and domain databases

InterProiIPR026160. Ric3.
[Graphical view]
PANTHERiPTHR21723. PTHR21723. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Cerebellum, Eye and Retina.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Conservation within the RIC-3 gene family. Effectors of mammalian nicotinic acetylcholine receptor expression."
    Halevi S., Yassin L., Eshel M., Sala F., Sala S., Criado M., Treinin M.
    J. Biol. Chem. 278:34411-34417(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Mouse RIC-3, an endoplasmic reticulum chaperone, promotes assembly of the alpha7 acetylcholine receptor through a cytoplasmic coiled-coil domain."
    Wang Y., Yao Y., Tang X.Q., Wang Z.Z.
    J. Neurosci. 29:12625-12635(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY, FUNCTION, COILED-COIL REGION, SIGNAL SEQUENCE CLEAVAGE SITE.

Entry informationi

Entry nameiRIC3_MOUSE
AccessioniPrimary (citable) accession number: Q8BPM6
Secondary accession number(s): Q6PCM7, Q8C4G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: March 4, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.