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Q8BPM2 (M4K5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase kinase kinase kinase 5

EC=2.7.11.1
Alternative name(s):
MAPK/ERK kinase kinase kinase 5
Short name=MEK kinase kinase 5
Short name=MEKKK 5
Gene names
Name:Map4k5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length847 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway By similarity. UniProtKB Q9Y4K4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB Q9Y4K4

Cofactor

Magnesium By similarity. UniProtKB Q9Y4K4

Subunit structure

Interacts with both SH3 domains of the adapter proteins CRK and CRKL By similarity. UniProtKB Q9Y4K4

Subcellular location

Cytoplasm By similarity UniProtKB Q9Y4K4.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Contains 1 CNH domain.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BPM2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BPM2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     294-312: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 847847Mitogen-activated protein kinase kinase kinase kinase 5
PRO_0000086283

Regions

Domain20 – 277258Protein kinase
Domain507 – 820314CNH
Nucleotide binding26 – 349ATP By similarity UniProtKB O00506

Sites

Active site1401Proton acceptor By similarity UniProtKB O00506
Binding site491ATP By similarity UniProtKB Q9Y4K4

Amino acid modifications

Modified residue3351Phosphoserine Ref.3

Natural variations

Alternative sequence294 – 31219Missing in isoform 2.
VSP_050478

Experimental info

Sequence conflict791A → P in BAC35517. Ref.1
Sequence conflict1031Q → H in BAC35517. Ref.1
Sequence conflict1181A → P in BAC35517. Ref.1
Sequence conflict4411A → V in AAH40381. Ref.2
Sequence conflict7271L → Q in BAB31739. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 30, 2003. Version 2.
Checksum: 377F0EFFC6EECFA3

FASTA84795,045
        10         20         30         40         50         60 
MEAPLRPAAD ILRRNPQHDY ELVQRVGSGT YGDVYKARNV HTGELAAVKI IKLEPGDDFS 

        70         80         90        100        110        120 
LIQQEIFMVK ECKHCNIVAY FGSYLSREKL WICMEYCGGG SLQDIYHVTG PLSEMQIAYV 

       130        140        150        160        170        180 
CRETLQGLAY LHTKGKMHRD IKGANILLTD HGDVKLADFG VAAKITATIA KRKSFIGTPY 

       190        200        210        220        230        240 
WMAPEVAAVE KNGGYNQLCD IWAVGITAIE LGELQPPMFD LHPMRALFLM SKSNFQPPKL 

       250        260        270        280        290        300 
KDKTKWSSTF HNFVKIALTK NPKKRPTAER LLTHTFVGQP GLSRALAVEL LDKVSNPDNH 

       310        320        330        340        350        360 
APYSEGDEDD LEPHAIIRHT IRSTNRNSRA ERTASEINFD KLQFEPPLRK ETEARDEMGL 

       370        380        390        400        410        420 
SSEPNFILHW NPFVDGANTG RSTSKRAIPP PLPPKPRVNT YPEDSLPDEE KSSTIKRCPD 

       430        440        450        460        470        480 
LEARAPQVLR RQSSPSCVPV AETSSSIGNG DGISKLISEN TEGSAQAPQL PRKKDKRDFP 

       490        500        510        520        530        540 
KPTINGLPPT PKVLMGACFS KVFDGCPLKI NCATSWIHPD TKDQYIIFGT EDGIYTLNLN 

       550        560        570        580        590        600 
ELHEATMEQL FPRKCTWLYV INNTLMSLSE GKTFQLYSHN LIALFEQAKK PGLAAHIQTH 

       610        620        630        640        650        660 
RFPDRILPRK FALTTKIPDT KGCHKCCIVR NPYTGHKYLC GALQSGIVLL QWYEPMQKFM 

       670        680        690        700        710        720 
LIKHFDFPLP SPLNVFEMLV IPEQEYPMVC VAISKGSDSS QVVQFETINL NSASSWFTEI 

       730        740        750        760        770        780 
GAGSQQLDSI HVTQLERDTV LVCLDKFVKI VNLQGKLKSS KKLASELSFD FRIESVVCLQ 

       790        800        810        820        830        840 
DSVLAFWKHG MQGKSFKSDE VTQEISDETR VFRLLGSDRV VVLESRPTEN PAAHSNLYIL 


AGHENSY 

« Hide

Isoform 2 [UniParc].

Checksum: 53F3939E68AEA591
Show »

FASTA82892,960

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Fetal eye, Fetal liver and Fetal lung.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Czech II.
Tissue: Mammary gland.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK019468 mRNA. Translation: BAB31739.1.
AK053775 mRNA. Translation: BAC35517.1.
AK084891 mRNA. Translation: BAC39305.1.
BC002309 mRNA. Translation: AAH02309.1.
BC040381 mRNA. Translation: AAH40381.2.
BC057930 mRNA. Translation: AAH57930.1.
RefSeqNP_958927.2. NM_201519.2. [Q8BPM2-1]
UniGeneMm.291936.

3D structure databases

ProteinModelPortalQ8BPM2.
SMRQ8BPM2. Positions 13-293.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8BPM2.

Proteomic databases

MaxQBQ8BPM2.
PaxDbQ8BPM2.
PRIDEQ8BPM2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000110567; ENSMUSP00000106196; ENSMUSG00000034761. [Q8BPM2-2]
ENSMUST00000110570; ENSMUSP00000106199; ENSMUSG00000034761. [Q8BPM2-1]
GeneID399510.
KEGGmmu:399510.
UCSCuc007nsu.1. mouse. [Q8BPM2-1]
uc011yne.1. mouse. [Q8BPM2-2]

Organism-specific databases

CTD11183.
MGIMGI:1925503. Map4k5.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00690000101936.
HOGENOMHOG000230824.
HOVERGENHBG036702.
InParanoidQ8BPM2.
KOK08833.
OMAYKARNVH.
OrthoDBEOG7WX07M.
PhylomeDBQ8BPM2.
TreeFamTF105121.

Gene expression databases

ArrayExpressQ8BPM2.
BgeeQ8BPM2.
GenevestigatorQ8BPM2.

Family and domain databases

InterProIPR001180. Citron.
IPR011009. Kinase-like_dom.
IPR021160. MAPKKKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF038172. MAPKKKK. 1 hit.
SMARTSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMAP4K5. mouse.
NextBio406008.
PROQ8BPM2.
SOURCESearch...

Entry information

Entry nameM4K5_MOUSE
AccessionPrimary (citable) accession number: Q8BPM2
Secondary accession number(s): Q6PEQ2 expand/collapse secondary AC list , Q8C3U5, Q8CGF3, Q99LM7, Q9CX73
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 14, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot