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Q8BPM2

- M4K5_MOUSE

UniProt

Q8BPM2 - M4K5_MOUSE

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Protein

Mitogen-activated protein kinase kinase kinase kinase 5

Gene

Map4k5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATPBy similarityPROSITE-ProRule annotation
Active sitei140 – 1401Proton acceptorBy similarityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATPBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. protein serine/threonine kinase activity Source: UniProtKB
  3. small GTPase regulator activity Source: InterPro

GO - Biological processi

  1. intracellular signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase kinase 5 (EC:2.7.11.1)
Alternative name(s):
MAPK/ERK kinase kinase kinase 5
Short name:
MEK kinase kinase 5
Short name:
MEKKK 5
Gene namesi
Name:Map4k5Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1925503. Map4k5.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 847847Mitogen-activated protein kinase kinase kinase kinase 5PRO_0000086283Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei335 – 3351Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BPM2.
PaxDbiQ8BPM2.
PRIDEiQ8BPM2.

PTM databases

PhosphoSiteiQ8BPM2.

Expressioni

Gene expression databases

BgeeiQ8BPM2.
ExpressionAtlasiQ8BPM2. baseline and differential.
GenevestigatoriQ8BPM2.

Interactioni

Subunit structurei

Interacts with both SH3 domains of the adapter proteins CRK and CRKL.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8BPM2.
SMRiQ8BPM2. Positions 13-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 277258Protein kinaseCuratedPROSITE-ProRule annotationAdd
BLAST
Domaini507 – 820314CNHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 CNH domain.CuratedPROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118848.
HOGENOMiHOG000230824.
HOVERGENiHBG036702.
InParanoidiQ8BPM2.
KOiK08833.
OMAiYKARNVH.
OrthoDBiEOG7WX07M.
PhylomeDBiQ8BPM2.
TreeFamiTF105121.

Family and domain databases

InterProiIPR001180. Citron.
IPR011009. Kinase-like_dom.
IPR021160. MAPKKKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF038172. MAPKKKK. 1 hit.
SMARTiSM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1Curated (identifier: Q8BPM2) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAPLRPAAD ILRRNPQHDY ELVQRVGSGT YGDVYKARNV HTGELAAVKI
60 70 80 90 100
IKLEPGDDFS LIQQEIFMVK ECKHCNIVAY FGSYLSREKL WICMEYCGGG
110 120 130 140 150
SLQDIYHVTG PLSEMQIAYV CRETLQGLAY LHTKGKMHRD IKGANILLTD
160 170 180 190 200
HGDVKLADFG VAAKITATIA KRKSFIGTPY WMAPEVAAVE KNGGYNQLCD
210 220 230 240 250
IWAVGITAIE LGELQPPMFD LHPMRALFLM SKSNFQPPKL KDKTKWSSTF
260 270 280 290 300
HNFVKIALTK NPKKRPTAER LLTHTFVGQP GLSRALAVEL LDKVSNPDNH
310 320 330 340 350
APYSEGDEDD LEPHAIIRHT IRSTNRNSRA ERTASEINFD KLQFEPPLRK
360 370 380 390 400
ETEARDEMGL SSEPNFILHW NPFVDGANTG RSTSKRAIPP PLPPKPRVNT
410 420 430 440 450
YPEDSLPDEE KSSTIKRCPD LEARAPQVLR RQSSPSCVPV AETSSSIGNG
460 470 480 490 500
DGISKLISEN TEGSAQAPQL PRKKDKRDFP KPTINGLPPT PKVLMGACFS
510 520 530 540 550
KVFDGCPLKI NCATSWIHPD TKDQYIIFGT EDGIYTLNLN ELHEATMEQL
560 570 580 590 600
FPRKCTWLYV INNTLMSLSE GKTFQLYSHN LIALFEQAKK PGLAAHIQTH
610 620 630 640 650
RFPDRILPRK FALTTKIPDT KGCHKCCIVR NPYTGHKYLC GALQSGIVLL
660 670 680 690 700
QWYEPMQKFM LIKHFDFPLP SPLNVFEMLV IPEQEYPMVC VAISKGSDSS
710 720 730 740 750
QVVQFETINL NSASSWFTEI GAGSQQLDSI HVTQLERDTV LVCLDKFVKI
760 770 780 790 800
VNLQGKLKSS KKLASELSFD FRIESVVCLQ DSVLAFWKHG MQGKSFKSDE
810 820 830 840
VTQEISDETR VFRLLGSDRV VVLESRPTEN PAAHSNLYIL AGHENSY
Length:847
Mass (Da):95,045
Last modified:April 30, 2003 - v2
Checksum:i377F0EFFC6EECFA3
GO
Isoform 2Curated (identifier: Q8BPM2-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     294-312: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:828
Mass (Da):92,960
Checksum:i53F3939E68AEA591
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti79 – 791A → P in BAC35517. (PubMed:16141072)Curated
Sequence conflicti103 – 1031Q → H in BAC35517. (PubMed:16141072)Curated
Sequence conflicti118 – 1181A → P in BAC35517. (PubMed:16141072)Curated
Sequence conflicti441 – 4411A → V in AAH40381. (PubMed:15489334)Curated
Sequence conflicti727 – 7271L → Q in BAB31739. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei294 – 31219Missing in isoform 2. 1 PublicationVSP_050478Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK019468 mRNA. Translation: BAB31739.1.
AK053775 mRNA. Translation: BAC35517.1.
AK084891 mRNA. Translation: BAC39305.1.
BC002309 mRNA. Translation: AAH02309.1.
BC040381 mRNA. Translation: AAH40381.2.
BC057930 mRNA. Translation: AAH57930.1.
RefSeqiNP_958927.2. NM_201519.2. [Q8BPM2-1]
UniGeneiMm.291936.

Genome annotation databases

EnsembliENSMUST00000110567; ENSMUSP00000106196; ENSMUSG00000034761. [Q8BPM2-2]
ENSMUST00000110570; ENSMUSP00000106199; ENSMUSG00000034761. [Q8BPM2-1]
GeneIDi399510.
KEGGimmu:399510.
UCSCiuc007nsu.1. mouse. [Q8BPM2-1]
uc011yne.1. mouse. [Q8BPM2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK019468 mRNA. Translation: BAB31739.1 .
AK053775 mRNA. Translation: BAC35517.1 .
AK084891 mRNA. Translation: BAC39305.1 .
BC002309 mRNA. Translation: AAH02309.1 .
BC040381 mRNA. Translation: AAH40381.2 .
BC057930 mRNA. Translation: AAH57930.1 .
RefSeqi NP_958927.2. NM_201519.2. [Q8BPM2-1 ]
UniGenei Mm.291936.

3D structure databases

ProteinModelPortali Q8BPM2.
SMRi Q8BPM2. Positions 13-293.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8BPM2.

Proteomic databases

MaxQBi Q8BPM2.
PaxDbi Q8BPM2.
PRIDEi Q8BPM2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000110567 ; ENSMUSP00000106196 ; ENSMUSG00000034761 . [Q8BPM2-2 ]
ENSMUST00000110570 ; ENSMUSP00000106199 ; ENSMUSG00000034761 . [Q8BPM2-1 ]
GeneIDi 399510.
KEGGi mmu:399510.
UCSCi uc007nsu.1. mouse. [Q8BPM2-1 ]
uc011yne.1. mouse. [Q8BPM2-2 ]

Organism-specific databases

CTDi 11183.
MGIi MGI:1925503. Map4k5.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000118848.
HOGENOMi HOG000230824.
HOVERGENi HBG036702.
InParanoidi Q8BPM2.
KOi K08833.
OMAi YKARNVH.
OrthoDBi EOG7WX07M.
PhylomeDBi Q8BPM2.
TreeFami TF105121.

Miscellaneous databases

ChiTaRSi MAP4K5. mouse.
NextBioi 406008.
PROi Q8BPM2.
SOURCEi Search...

Gene expression databases

Bgeei Q8BPM2.
ExpressionAtlasi Q8BPM2. baseline and differential.
Genevestigatori Q8BPM2.

Family and domain databases

InterProi IPR001180. Citron.
IPR011009. Kinase-like_dom.
IPR021160. MAPKKKK.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view ]
Pfami PF00780. CNH. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF038172. MAPKKKK. 1 hit.
SMARTi SM00036. CNH. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS50219. CNH. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Fetal eye, Fetal liver and Fetal lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.
    Tissue: Mammary gland.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiM4K5_MOUSE
AccessioniPrimary (citable) accession number: Q8BPM2
Secondary accession number(s): Q6PEQ2
, Q8C3U5, Q8CGF3, Q99LM7, Q9CX73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: October 29, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3