Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8BPM2

- M4K5_MOUSE

UniProt

Q8BPM2 - M4K5_MOUSE

Protein

Mitogen-activated protein kinase kinase kinase kinase 5

Gene

Map4k5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (30 Apr 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491ATPBy similarityPROSITE-ProRule annotation
    Active sitei140 – 1401Proton acceptorBy similarityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 349ATPBy similarityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB
    2. protein serine/threonine kinase activity Source: UniProtKB
    3. small GTPase regulator activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase kinase kinase kinase 5 (EC:2.7.11.1)
    Alternative name(s):
    MAPK/ERK kinase kinase kinase 5
    Short name:
    MEK kinase kinase 5
    Short name:
    MEKKK 5
    Gene namesi
    Name:Map4k5Imported
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1925503. Map4k5.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 847847Mitogen-activated protein kinase kinase kinase kinase 5PRO_0000086283Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei335 – 3351Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8BPM2.
    PaxDbiQ8BPM2.
    PRIDEiQ8BPM2.

    PTM databases

    PhosphoSiteiQ8BPM2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BPM2.
    BgeeiQ8BPM2.
    GenevestigatoriQ8BPM2.

    Interactioni

    Subunit structurei

    Interacts with both SH3 domains of the adapter proteins CRK and CRKL.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BPM2.
    SMRiQ8BPM2. Positions 13-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 277258Protein kinaseCuratedPROSITE-ProRule annotationAdd
    BLAST
    Domaini507 – 820314CNHPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CNH domain.CuratedPROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00690000101936.
    HOGENOMiHOG000230824.
    HOVERGENiHBG036702.
    InParanoidiQ8BPM2.
    KOiK08833.
    OMAiYKARNVH.
    OrthoDBiEOG7WX07M.
    PhylomeDBiQ8BPM2.
    TreeFamiTF105121.

    Family and domain databases

    InterProiIPR001180. Citron.
    IPR011009. Kinase-like_dom.
    IPR021160. MAPKKKK.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00780. CNH. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF038172. MAPKKKK. 1 hit.
    SMARTiSM00036. CNH. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS50219. CNH. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1Curated (identifier: Q8BPM2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEAPLRPAAD ILRRNPQHDY ELVQRVGSGT YGDVYKARNV HTGELAAVKI    50
    IKLEPGDDFS LIQQEIFMVK ECKHCNIVAY FGSYLSREKL WICMEYCGGG 100
    SLQDIYHVTG PLSEMQIAYV CRETLQGLAY LHTKGKMHRD IKGANILLTD 150
    HGDVKLADFG VAAKITATIA KRKSFIGTPY WMAPEVAAVE KNGGYNQLCD 200
    IWAVGITAIE LGELQPPMFD LHPMRALFLM SKSNFQPPKL KDKTKWSSTF 250
    HNFVKIALTK NPKKRPTAER LLTHTFVGQP GLSRALAVEL LDKVSNPDNH 300
    APYSEGDEDD LEPHAIIRHT IRSTNRNSRA ERTASEINFD KLQFEPPLRK 350
    ETEARDEMGL SSEPNFILHW NPFVDGANTG RSTSKRAIPP PLPPKPRVNT 400
    YPEDSLPDEE KSSTIKRCPD LEARAPQVLR RQSSPSCVPV AETSSSIGNG 450
    DGISKLISEN TEGSAQAPQL PRKKDKRDFP KPTINGLPPT PKVLMGACFS 500
    KVFDGCPLKI NCATSWIHPD TKDQYIIFGT EDGIYTLNLN ELHEATMEQL 550
    FPRKCTWLYV INNTLMSLSE GKTFQLYSHN LIALFEQAKK PGLAAHIQTH 600
    RFPDRILPRK FALTTKIPDT KGCHKCCIVR NPYTGHKYLC GALQSGIVLL 650
    QWYEPMQKFM LIKHFDFPLP SPLNVFEMLV IPEQEYPMVC VAISKGSDSS 700
    QVVQFETINL NSASSWFTEI GAGSQQLDSI HVTQLERDTV LVCLDKFVKI 750
    VNLQGKLKSS KKLASELSFD FRIESVVCLQ DSVLAFWKHG MQGKSFKSDE 800
    VTQEISDETR VFRLLGSDRV VVLESRPTEN PAAHSNLYIL AGHENSY 847
    Length:847
    Mass (Da):95,045
    Last modified:April 30, 2003 - v2
    Checksum:i377F0EFFC6EECFA3
    GO
    Isoform 2Curated (identifier: Q8BPM2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         294-312: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:828
    Mass (Da):92,960
    Checksum:i53F3939E68AEA591
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti79 – 791A → P in BAC35517. (PubMed:16141072)Curated
    Sequence conflicti103 – 1031Q → H in BAC35517. (PubMed:16141072)Curated
    Sequence conflicti118 – 1181A → P in BAC35517. (PubMed:16141072)Curated
    Sequence conflicti441 – 4411A → V in AAH40381. (PubMed:15489334)Curated
    Sequence conflicti727 – 7271L → Q in BAB31739. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei294 – 31219Missing in isoform 2. 1 PublicationVSP_050478Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK019468 mRNA. Translation: BAB31739.1.
    AK053775 mRNA. Translation: BAC35517.1.
    AK084891 mRNA. Translation: BAC39305.1.
    BC002309 mRNA. Translation: AAH02309.1.
    BC040381 mRNA. Translation: AAH40381.2.
    BC057930 mRNA. Translation: AAH57930.1.
    RefSeqiNP_958927.2. NM_201519.2. [Q8BPM2-1]
    UniGeneiMm.291936.

    Genome annotation databases

    EnsembliENSMUST00000110567; ENSMUSP00000106196; ENSMUSG00000034761. [Q8BPM2-2]
    ENSMUST00000110570; ENSMUSP00000106199; ENSMUSG00000034761. [Q8BPM2-1]
    GeneIDi399510.
    KEGGimmu:399510.
    UCSCiuc007nsu.1. mouse. [Q8BPM2-1]
    uc011yne.1. mouse. [Q8BPM2-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK019468 mRNA. Translation: BAB31739.1 .
    AK053775 mRNA. Translation: BAC35517.1 .
    AK084891 mRNA. Translation: BAC39305.1 .
    BC002309 mRNA. Translation: AAH02309.1 .
    BC040381 mRNA. Translation: AAH40381.2 .
    BC057930 mRNA. Translation: AAH57930.1 .
    RefSeqi NP_958927.2. NM_201519.2. [Q8BPM2-1 ]
    UniGenei Mm.291936.

    3D structure databases

    ProteinModelPortali Q8BPM2.
    SMRi Q8BPM2. Positions 13-293.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BPM2.

    Proteomic databases

    MaxQBi Q8BPM2.
    PaxDbi Q8BPM2.
    PRIDEi Q8BPM2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000110567 ; ENSMUSP00000106196 ; ENSMUSG00000034761 . [Q8BPM2-2 ]
    ENSMUST00000110570 ; ENSMUSP00000106199 ; ENSMUSG00000034761 . [Q8BPM2-1 ]
    GeneIDi 399510.
    KEGGi mmu:399510.
    UCSCi uc007nsu.1. mouse. [Q8BPM2-1 ]
    uc011yne.1. mouse. [Q8BPM2-2 ]

    Organism-specific databases

    CTDi 11183.
    MGIi MGI:1925503. Map4k5.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00690000101936.
    HOGENOMi HOG000230824.
    HOVERGENi HBG036702.
    InParanoidi Q8BPM2.
    KOi K08833.
    OMAi YKARNVH.
    OrthoDBi EOG7WX07M.
    PhylomeDBi Q8BPM2.
    TreeFami TF105121.

    Miscellaneous databases

    ChiTaRSi MAP4K5. mouse.
    NextBioi 406008.
    PROi Q8BPM2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BPM2.
    Bgeei Q8BPM2.
    Genevestigatori Q8BPM2.

    Family and domain databases

    InterProi IPR001180. Citron.
    IPR011009. Kinase-like_dom.
    IPR021160. MAPKKKK.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00780. CNH. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF038172. MAPKKKK. 1 hit.
    SMARTi SM00036. CNH. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS50219. CNH. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Fetal eye, Fetal liver and Fetal lung.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Czech II.
      Tissue: Mammary gland.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-335, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiM4K5_MOUSE
    AccessioniPrimary (citable) accession number: Q8BPM2
    Secondary accession number(s): Q6PEQ2
    , Q8C3U5, Q8CGF3, Q99LM7, Q9CX73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3