UniProtKB - Q8BPM0 (DAAM1_MOUSE)
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Protein
Disheveled-associated activator of morphogenesis 1
Gene
Daam1
Organism
Mus musculus (Mouse)
Status
Functioni
Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity). Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (PubMed:26526197).By similarity1 Publication
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- identical protein binding Source: MGI
- Rho GTPase binding Source: InterPro
GO - Biological processi
- actin cytoskeleton organization Source: InterPro
Keywordsi
Molecular function | Actin-binding |
Biological process | Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-MMU-4086400. PCP/CE pathway. R-MMU-5663220. RHO GTPases Activate Formins. |
Names & Taxonomyi
Protein namesi | Recommended name: Disheveled-associated activator of morphogenesis 1 |
Gene namesi | Name:Daam1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914596. Daam1. |
Pathology & Biotechi
Disruption phenotypei
Conditional knockout mice lacking Daam1 in myocardial cells show cardiomyopathy. Conditional knockout mice lacking Daam1 and Daam2 in myocardial cells show stronger cardiomyopathy.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194908 | 1 – 1077 | Disheveled-associated activator of morphogenesis 1Add BLAST | 1077 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1026 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1029 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q8BPM0. |
PeptideAtlasi | Q8BPM0. |
PRIDEi | Q8BPM0. |
PTM databases
iPTMneti | Q8BPM0. |
PhosphoSitePlusi | Q8BPM0. |
SwissPalmi | Q8BPM0. |
Expressioni
Tissue specificityi
In early embryogenesis, expressed in embryonic and extraembryonic ectoderm. In later stages of gastrulation, expressed also in somites and ribs and posterior vertebrae of developing skeletal system. During organogenesis, expressed in CNS, PNS, stomach, liver and limb bud.2 Publications
Developmental stagei
Detected throughout the myocardial layer of the heart tube. Not expressed in the myocardium at E9.5 but is present in epicardial cells and the pro-epicardial organ. At E10.5, expressed in the atrial and ventricular myocardia as well as the inter-ventricular septum. Continues to localize to the atrial and ventricular myocardia at E12.5 as well as the ventricular trabeculae.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000034574. |
Genevisiblei | Q8BPM0. MM. |
Interactioni
Subunit structurei
Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA. Interacts with INTU; INTU mediates the indirect interaction between DAAM1 and NPHP4 (By similarity).By similarity
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
ARHGEF11 | O15085-2 | 3 | EBI-772938,EBI-6169263 | From a different organism. |
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- identical protein binding Source: MGI
- Rho GTPase binding Source: InterPro
Protein-protein interaction databases
BioGridi | 229018. 1 interactor. |
IntActi | Q8BPM0. 2 interactors. |
STRINGi | 10090.ENSMUSP00000082406. |
Structurei
3D structure databases
ProteinModelPortali | Q8BPM0. |
SMRi | Q8BPM0. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 45 – 420 | GBD/FH3PROSITE-ProRule annotationAdd BLAST | 376 | |
Domaini | 528 – 599 | FH1Add BLAST | 72 | |
Domaini | 600 – 1008 | FH2PROSITE-ProRule annotationAdd BLAST | 409 | |
Domaini | 1026 – 1057 | DADPROSITE-ProRule annotationAdd BLAST | 32 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 693 – 702 | Actin-bindingBy similarity | 10 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 437 – 526 | Sequence analysisAdd BLAST | 90 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 528 – 593 | Pro-richAdd BLAST | 66 |
Domaini
The C-terminal DAD domain may participate in intramolecular interactions with the N-terminus.
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity
Sequence similaritiesi
Belongs to the formin homology family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG1922. Eukaryota. ENOG410XT5Z. LUCA. |
GeneTreei | ENSGT00760000118986. |
HOGENOMi | HOG000237318. |
HOVERGENi | HBG101333. |
InParanoidi | Q8BPM0. |
KOi | K04512. |
OMAi | PLGGIMP. |
OrthoDBi | EOG091G02QJ. |
TreeFami | TF314602. |
Family and domain databases
Gene3Di | 1.25.10.10. 1 hit. |
InterProi | View protein in InterPro IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR027650. DAAM1. IPR014767. DAD_dom. IPR015425. FH2_Formin. IPR010472. FH3_dom. IPR014768. GBD/FH3_dom. IPR010473. GTPase-bd. |
PANTHERi | PTHR23213:SF181. PTHR23213:SF181. 1 hit. |
Pfami | View protein in Pfam PF06367. Drf_FH3. 1 hit. PF06371. Drf_GBD. 1 hit. PF02181. FH2. 1 hit. |
SMARTi | View protein in SMART SM01139. Drf_FH3. 1 hit. SM01140. Drf_GBD. 1 hit. SM00498. FH2. 1 hit. |
SUPFAMi | SSF48371. SSF48371. 1 hit. |
PROSITEi | View protein in PROSITE PS51231. DAD. 1 hit. PS51444. FH2. 1 hit. PS51232. GBD_FH3. 1 hit. |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q8BPM0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV
60 70 80 90 100
EELDVMFSEL VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT
110 120 130 140 150
SWPEFYIDQL NSMAARKSLL ALEKEEEEER SKTIESLKTA LRTKPMRFVT
160 170 180 190 200
RFIDLDGLSC ILNFLKTMDY ETSESRIHTS LIGCIKALMN NSQGRAHVLA
210 220 230 240 250
HSESINVIAQ SLSTENIKTK VAVLEILGAV CLVPGGHKKV LQAMLHYQKY
260 270 280 290 300
ASERTRFQTL INDLDKSTGR YRDEVSLKTA IMSFINAVLS QGAGVESLDF
310 320 330 340 350
RLHLRYEFLM LGIQPVIDKL REHENSTLDR HLDFFEMLRN EDELEFAKRF
360 370 380 390 400
ELVHIDTKSA TQMFELTRRR LTHSEAYPHF MSILHHCLQM PYKRSGNTVQ
410 420 430 440 450
YWLLLDRIIQ QIVIQNDKGQ DPDSTPLENF NIKNVVRMLV NENEVKQWKE
460 470 480 490 500
QAEKMRKEHN ELQQKLEKKE RECDAKTQEK EEMMQTLNKM KEKLEKETTE
510 520 530 540 550
HKQVKQQVAD LTAQLHELNR RAVCAAVPGG PSPGAPGGPF PSSGLGSLLP
560 570 580 590 600
PPPPPLLSGG ALPPPPPPLP PGGPPPPPGP PPLGGVLPPP GAPVSLTLKK
610 620 630 640 650
KNIPQPTNAL KSFNWSKLPE NKLDGTVWTE IDDTKVFKIL DLEDLERTFS
660 670 680 690 700
AYQRQQEFFV NNSKQKEADA IDDTLSSKLK VKELSVIDGR RAQNCNILLS
710 720 730 740 750
RLKLSNDEIK RAILTMDEQE DLPKDMLEQL LKFVPEKSDI DLLEEHKHEL
760 770 780 790 800
DRMAKADRFL FEMSRINHYQ QRLQSLYFKK KFAERVAEVK PKVEAIRSGS
810 820 830 840 850
EEVFRSRALK QLLEVVLAFG NYMNKGQRGN AYGFKISSLN KIADTKSSID
860 870 880 890 900
KNITLLHYLI TIVENKYPKV LNLSEELRDI PQAAKVNMTE LDKEISTLRS
910 920 930 940 950
GLKAVETELE YQKSQPPQPG DKFVSVVSQF ITLASFSFSD VEDLLAEAKE
960 970 980 990 1000
LFTKAVKHFG EEAGKIQPDE FFGIFDQFLQ AVAEAKQENE NMRKRKEEEE
1010 1020 1030 1040 1050
RRARLEAQLK EQRERERKVR KAKESSEESG EFDDLVSALR SGEVFDKDLS
1060 1070
KLKRNRKRIS NQVTDSSRER PITKLNF
Sequence cautioni
The sequence AAH48856 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 | F → L in AAR05118 (PubMed:15533824).Curated | 1 | |
Sequence conflicti | 571 | P → A in BAC35522 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 894 | E → G in BAE27943 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_027771 | 657 – 665 | Missing in isoform 2. 2 Publications | 9 | |
Alternative sequenceiVSP_027772 | 657 – 659 | EFF → VTT in isoform 3. 1 Publication | 3 | |
Alternative sequenceiVSP_027773 | 660 – 1077 | Missing in isoform 3. 1 PublicationAdd BLAST | 418 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY426535 mRNA. Translation: AAR05118.1. AK006902 mRNA. Translation: BAB24785.1. AK018919 mRNA. Translation: BAB31482.1. AK018950 mRNA. Translation: BAB31491.1. AK053785 mRNA. Translation: BAC35522.1. AK147480 mRNA. Translation: BAE27943.1. BC032287 mRNA. No translation available. BC048856 mRNA. Translation: AAH48856.1. Different initiation. BC076585 mRNA. Translation: AAH76585.1. |
CCDSi | CCDS25964.1. [Q8BPM0-1] |
RefSeqi | NP_001273381.1. NM_001286452.1. [Q8BPM0-2] NP_080378.2. NM_026102.3. [Q8BPM0-1] NP_766052.2. NM_172464.3. [Q8BPM0-1] XP_006515705.1. XM_006515642.2. [Q8BPM0-1] XP_006515706.1. XM_006515643.3. [Q8BPM0-2] XP_006515707.1. XM_006515644.3. [Q8BPM0-2] |
UniGenei | Mm.474935. Mm.87417. |
Genome annotation databases
Ensembli | ENSMUST00000085299; ENSMUSP00000082406; ENSMUSG00000034574. [Q8BPM0-1] ENSMUST00000221317; ENSMUSP00000152532; ENSMUSG00000034574. [Q8BPM0-2] ENSMUST00000223272; ENSMUSP00000152564; ENSMUSG00000034574. [Q8BPM0-1] |
GeneIDi | 208846. |
KEGGi | mmu:208846. |
UCSCi | uc007nuw.2. mouse. [Q8BPM0-3] uc007nux.2. mouse. [Q8BPM0-1] uc007nuz.2. mouse. [Q8BPM0-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Entry informationi
Entry namei | DAAM1_MOUSE | |
Accessioni | Q8BPM0Primary (citable) accession number: Q8BPM0 Secondary accession number(s): Q3UHB4 Q9CQQ2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 15, 2003 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 28, 2018 | |
This is version 130 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |