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Protein

Disheveled-associated activator of morphogenesis 1

Gene

Daam1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity). Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (PubMed:26526197).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-4086400 PCP/CE pathway
R-MMU-5663220 RHO GTPases Activate Formins

Names & Taxonomyi

Protein namesi
Recommended name:
Disheveled-associated activator of morphogenesis 1
Gene namesi
Name:Daam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1914596 Daam1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Conditional knockout mice lacking Daam1 in myocardial cells show cardiomyopathy. Conditional knockout mice lacking Daam1 and Daam2 in myocardial cells show stronger cardiomyopathy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949081 – 1077Disheveled-associated activator of morphogenesis 1Add BLAST1077

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineBy similarity1
Modified residuei1026PhosphoserineBy similarity1
Modified residuei1029PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BPM0
PeptideAtlasiQ8BPM0
PRIDEiQ8BPM0

PTM databases

iPTMnetiQ8BPM0
PhosphoSitePlusiQ8BPM0
SwissPalmiQ8BPM0

Expressioni

Tissue specificityi

In early embryogenesis, expressed in embryonic and extraembryonic ectoderm. In later stages of gastrulation, expressed also in somites and ribs and posterior vertebrae of developing skeletal system. During organogenesis, expressed in CNS, PNS, stomach, liver and limb bud.2 Publications

Developmental stagei

Detected throughout the myocardial layer of the heart tube. Not expressed in the myocardium at E9.5 but is present in epicardial cells and the pro-epicardial organ. At E10.5, expressed in the atrial and ventricular myocardia as well as the inter-ventricular septum. Continues to localize to the atrial and ventricular myocardia at E12.5 as well as the ventricular trabeculae.1 Publication

Gene expression databases

BgeeiENSMUSG00000034574
GenevisibleiQ8BPM0 MM

Interactioni

Subunit structurei

Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA. Interacts with INTU; INTU mediates the indirect interaction between DAAM1 and NPHP4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ARHGEF11O15085-23EBI-772938,EBI-6169263From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229018, 1 interactor
IntActiQ8BPM0, 2 interactors
STRINGi10090.ENSMUSP00000082406

Structurei

3D structure databases

ProteinModelPortaliQ8BPM0
SMRiQ8BPM0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 420GBD/FH3PROSITE-ProRule annotationAdd BLAST376
Domaini528 – 599FH1Add BLAST72
Domaini600 – 1008FH2PROSITE-ProRule annotationAdd BLAST409
Domaini1026 – 1057DADPROSITE-ProRule annotationAdd BLAST32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni693 – 702Actin-bindingBy similarity10

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili437 – 526Sequence analysisAdd BLAST90

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi528 – 593Pro-richAdd BLAST66

Domaini

The C-terminal DAD domain may participate in intramolecular interactions with the N-terminus.
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922 Eukaryota
ENOG410XT5Z LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000237318
HOVERGENiHBG101333
InParanoidiQ8BPM0
KOiK04512
OMAiPLGGIMP
OrthoDBiEOG091G02QJ
TreeFamiTF314602

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR027650 DAAM1
IPR014767 DAD_dom
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PANTHERiPTHR23213:SF181 PTHR23213:SF181, 1 hit
PfamiView protein in Pfam
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51231 DAD, 1 hit
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BPM0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV
60 70 80 90 100
EELDVMFSEL VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT
110 120 130 140 150
SWPEFYIDQL NSMAARKSLL ALEKEEEEER SKTIESLKTA LRTKPMRFVT
160 170 180 190 200
RFIDLDGLSC ILNFLKTMDY ETSESRIHTS LIGCIKALMN NSQGRAHVLA
210 220 230 240 250
HSESINVIAQ SLSTENIKTK VAVLEILGAV CLVPGGHKKV LQAMLHYQKY
260 270 280 290 300
ASERTRFQTL INDLDKSTGR YRDEVSLKTA IMSFINAVLS QGAGVESLDF
310 320 330 340 350
RLHLRYEFLM LGIQPVIDKL REHENSTLDR HLDFFEMLRN EDELEFAKRF
360 370 380 390 400
ELVHIDTKSA TQMFELTRRR LTHSEAYPHF MSILHHCLQM PYKRSGNTVQ
410 420 430 440 450
YWLLLDRIIQ QIVIQNDKGQ DPDSTPLENF NIKNVVRMLV NENEVKQWKE
460 470 480 490 500
QAEKMRKEHN ELQQKLEKKE RECDAKTQEK EEMMQTLNKM KEKLEKETTE
510 520 530 540 550
HKQVKQQVAD LTAQLHELNR RAVCAAVPGG PSPGAPGGPF PSSGLGSLLP
560 570 580 590 600
PPPPPLLSGG ALPPPPPPLP PGGPPPPPGP PPLGGVLPPP GAPVSLTLKK
610 620 630 640 650
KNIPQPTNAL KSFNWSKLPE NKLDGTVWTE IDDTKVFKIL DLEDLERTFS
660 670 680 690 700
AYQRQQEFFV NNSKQKEADA IDDTLSSKLK VKELSVIDGR RAQNCNILLS
710 720 730 740 750
RLKLSNDEIK RAILTMDEQE DLPKDMLEQL LKFVPEKSDI DLLEEHKHEL
760 770 780 790 800
DRMAKADRFL FEMSRINHYQ QRLQSLYFKK KFAERVAEVK PKVEAIRSGS
810 820 830 840 850
EEVFRSRALK QLLEVVLAFG NYMNKGQRGN AYGFKISSLN KIADTKSSID
860 870 880 890 900
KNITLLHYLI TIVENKYPKV LNLSEELRDI PQAAKVNMTE LDKEISTLRS
910 920 930 940 950
GLKAVETELE YQKSQPPQPG DKFVSVVSQF ITLASFSFSD VEDLLAEAKE
960 970 980 990 1000
LFTKAVKHFG EEAGKIQPDE FFGIFDQFLQ AVAEAKQENE NMRKRKEEEE
1010 1020 1030 1040 1050
RRARLEAQLK EQRERERKVR KAKESSEESG EFDDLVSALR SGEVFDKDLS
1060 1070
KLKRNRKRIS NQVTDSSRER PITKLNF
Length:1,077
Mass (Da):123,370
Last modified:July 27, 2011 - v4
Checksum:iE16AC9DEF6E05B15
GO
Isoform 2 (identifier: Q8BPM0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     657-665: Missing.

Show »
Length:1,068
Mass (Da):122,276
Checksum:iF5AD716B8D158DD3
GO
Isoform 3 (identifier: Q8BPM0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     657-659: EFF → VTT
     660-1077: Missing.

Note: No experimental confirmation available.
Show »
Length:659
Mass (Da):74,953
Checksum:i59B031884A39FEA5
GO

Sequence cautioni

The sequence AAH48856 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36F → L in AAR05118 (PubMed:15533824).Curated1
Sequence conflicti571P → A in BAC35522 (PubMed:16141072).Curated1
Sequence conflicti894E → G in BAE27943 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027771657 – 665Missing in isoform 2. 2 Publications9
Alternative sequenceiVSP_027772657 – 659EFF → VTT in isoform 3. 1 Publication3
Alternative sequenceiVSP_027773660 – 1077Missing in isoform 3. 1 PublicationAdd BLAST418

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY426535 mRNA Translation: AAR05118.1
AK006902 mRNA Translation: BAB24785.1
AK018919 mRNA Translation: BAB31482.1
AK018950 mRNA Translation: BAB31491.1
AK053785 mRNA Translation: BAC35522.1
AK147480 mRNA Translation: BAE27943.1
BC032287 mRNA No translation available.
BC048856 mRNA Translation: AAH48856.1 Different initiation.
BC076585 mRNA Translation: AAH76585.1
CCDSiCCDS25964.1 [Q8BPM0-1]
RefSeqiNP_001273381.1, NM_001286452.1 [Q8BPM0-2]
NP_080378.2, NM_026102.3 [Q8BPM0-1]
NP_766052.2, NM_172464.3 [Q8BPM0-1]
XP_006515705.1, XM_006515642.2 [Q8BPM0-1]
XP_006515706.1, XM_006515643.3 [Q8BPM0-2]
XP_006515707.1, XM_006515644.3 [Q8BPM0-2]
UniGeneiMm.474935
Mm.87417

Genome annotation databases

EnsembliENSMUST00000085299; ENSMUSP00000082406; ENSMUSG00000034574 [Q8BPM0-1]
ENSMUST00000221317; ENSMUSP00000152532; ENSMUSG00000034574 [Q8BPM0-2]
ENSMUST00000223272; ENSMUSP00000152564; ENSMUSG00000034574 [Q8BPM0-1]
GeneIDi208846
KEGGimmu:208846
UCSCiuc007nuw.2 mouse [Q8BPM0-3]
uc007nux.2 mouse [Q8BPM0-1]
uc007nuz.2 mouse [Q8BPM0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDAAM1_MOUSE
AccessioniPrimary (citable) accession number: Q8BPM0
Secondary accession number(s): Q3UHB4
, Q6DFY0, Q6TAB8, Q80Y68, Q9CQQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 131 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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