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Protein

Disheveled-associated activator of morphogenesis 1

Gene

Daam1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-MMU-4086400. PCP/CE pathway.
R-MMU-5663220. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Disheveled-associated activator of morphogenesis 1
Gene namesi
Name:Daam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1914596. Daam1.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Perinuclear.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • membrane Source: MGI
  • plasma membrane Source: MGI
  • stress fiber Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10771077Disheveled-associated activator of morphogenesis 1PRO_0000194908Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341PhosphoserineBy similarity
Modified residuei1026 – 10261PhosphoserineBy similarity
Modified residuei1029 – 10291PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8BPM0.
PaxDbiQ8BPM0.
PeptideAtlasiQ8BPM0.
PRIDEiQ8BPM0.

PTM databases

iPTMnetiQ8BPM0.
PhosphoSiteiQ8BPM0.
SwissPalmiQ8BPM0.

Expressioni

Tissue specificityi

In early embryogenesis, expressed in embryonic and extraembryonic ectoderm. In later stages of gastrulation, expressed also in somites and ribs and posterior vertebrae of developing skeletal system. During organogenesis, expressed in CNS, PNS, stomach, liver and limb bud.2 Publications

Gene expression databases

BgeeiENSMUSG00000034574.
GenevisibleiQ8BPM0. MM.

Interactioni

Subunit structurei

Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ARHGEF11O15085-23EBI-772938,EBI-6169263From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8BPM0. 2 interactions.
STRINGi10090.ENSMUSP00000082406.

Structurei

3D structure databases

ProteinModelPortaliQ8BPM0.
SMRiQ8BPM0. Positions 52-446, 601-1047.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 420376GBD/FH3PROSITE-ProRule annotationAdd
BLAST
Domaini528 – 59972FH1Add
BLAST
Domaini600 – 1008409FH2PROSITE-ProRule annotationAdd
BLAST
Domaini1026 – 105732DADPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni693 – 70210Actin-bindingBy similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili437 – 52690Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi528 – 59366Pro-richAdd
BLAST

Domaini

The C-terminal DAD domain may participate in intramolecular interactions with the N-terminus.
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated
Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
ENOG410XT5Z. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000237318.
HOVERGENiHBG101333.
InParanoidiQ8BPM0.
KOiK04512.
OMAiWNQYSGR.
OrthoDBiEOG091G02QJ.
TreeFamiTF314602.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027650. DAAM1.
IPR014767. DAD_dom.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PANTHERiPTHR23213:SF181. PTHR23213:SF181. 2 hits.
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BPM0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV
60 70 80 90 100
EELDVMFSEL VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT
110 120 130 140 150
SWPEFYIDQL NSMAARKSLL ALEKEEEEER SKTIESLKTA LRTKPMRFVT
160 170 180 190 200
RFIDLDGLSC ILNFLKTMDY ETSESRIHTS LIGCIKALMN NSQGRAHVLA
210 220 230 240 250
HSESINVIAQ SLSTENIKTK VAVLEILGAV CLVPGGHKKV LQAMLHYQKY
260 270 280 290 300
ASERTRFQTL INDLDKSTGR YRDEVSLKTA IMSFINAVLS QGAGVESLDF
310 320 330 340 350
RLHLRYEFLM LGIQPVIDKL REHENSTLDR HLDFFEMLRN EDELEFAKRF
360 370 380 390 400
ELVHIDTKSA TQMFELTRRR LTHSEAYPHF MSILHHCLQM PYKRSGNTVQ
410 420 430 440 450
YWLLLDRIIQ QIVIQNDKGQ DPDSTPLENF NIKNVVRMLV NENEVKQWKE
460 470 480 490 500
QAEKMRKEHN ELQQKLEKKE RECDAKTQEK EEMMQTLNKM KEKLEKETTE
510 520 530 540 550
HKQVKQQVAD LTAQLHELNR RAVCAAVPGG PSPGAPGGPF PSSGLGSLLP
560 570 580 590 600
PPPPPLLSGG ALPPPPPPLP PGGPPPPPGP PPLGGVLPPP GAPVSLTLKK
610 620 630 640 650
KNIPQPTNAL KSFNWSKLPE NKLDGTVWTE IDDTKVFKIL DLEDLERTFS
660 670 680 690 700
AYQRQQEFFV NNSKQKEADA IDDTLSSKLK VKELSVIDGR RAQNCNILLS
710 720 730 740 750
RLKLSNDEIK RAILTMDEQE DLPKDMLEQL LKFVPEKSDI DLLEEHKHEL
760 770 780 790 800
DRMAKADRFL FEMSRINHYQ QRLQSLYFKK KFAERVAEVK PKVEAIRSGS
810 820 830 840 850
EEVFRSRALK QLLEVVLAFG NYMNKGQRGN AYGFKISSLN KIADTKSSID
860 870 880 890 900
KNITLLHYLI TIVENKYPKV LNLSEELRDI PQAAKVNMTE LDKEISTLRS
910 920 930 940 950
GLKAVETELE YQKSQPPQPG DKFVSVVSQF ITLASFSFSD VEDLLAEAKE
960 970 980 990 1000
LFTKAVKHFG EEAGKIQPDE FFGIFDQFLQ AVAEAKQENE NMRKRKEEEE
1010 1020 1030 1040 1050
RRARLEAQLK EQRERERKVR KAKESSEESG EFDDLVSALR SGEVFDKDLS
1060 1070
KLKRNRKRIS NQVTDSSRER PITKLNF
Length:1,077
Mass (Da):123,370
Last modified:July 27, 2011 - v4
Checksum:iE16AC9DEF6E05B15
GO
Isoform 2 (identifier: Q8BPM0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     657-665: Missing.

Show »
Length:1,068
Mass (Da):122,276
Checksum:iF5AD716B8D158DD3
GO
Isoform 3 (identifier: Q8BPM0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     657-659: EFF → VTT
     660-1077: Missing.

Note: No experimental confirmation available.
Show »
Length:659
Mass (Da):74,953
Checksum:i59B031884A39FEA5
GO

Sequence cautioni

The sequence AAH48856 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361F → L in AAR05118 (PubMed:15533824).Curated
Sequence conflicti571 – 5711P → A in BAC35522 (PubMed:16141072).Curated
Sequence conflicti894 – 8941E → G in BAE27943 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei657 – 6659Missing in isoform 2. 2 PublicationsVSP_027771
Alternative sequencei657 – 6593EFF → VTT in isoform 3. 1 PublicationVSP_027772
Alternative sequencei660 – 1077418Missing in isoform 3. 1 PublicationVSP_027773Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY426535 mRNA. Translation: AAR05118.1.
AK006902 mRNA. Translation: BAB24785.1.
AK018919 mRNA. Translation: BAB31482.1.
AK018950 mRNA. Translation: BAB31491.1.
AK053785 mRNA. Translation: BAC35522.1.
AK147480 mRNA. Translation: BAE27943.1.
BC032287 mRNA. No translation available.
BC048856 mRNA. Translation: AAH48856.1. Different initiation.
BC076585 mRNA. Translation: AAH76585.1.
CCDSiCCDS25964.1. [Q8BPM0-1]
RefSeqiNP_001273381.1. NM_001286452.1. [Q8BPM0-2]
NP_080378.2. NM_026102.3. [Q8BPM0-1]
NP_766052.2. NM_172464.3. [Q8BPM0-1]
XP_006515705.1. XM_006515642.2. [Q8BPM0-1]
XP_006515706.1. XM_006515643.2. [Q8BPM0-2]
XP_006515707.1. XM_006515644.2. [Q8BPM0-2]
UniGeneiMm.474935.
Mm.87417.

Genome annotation databases

EnsembliENSMUST00000085299; ENSMUSP00000082406; ENSMUSG00000034574. [Q8BPM0-1]
GeneIDi208846.
KEGGimmu:208846.
UCSCiuc007nuw.2. mouse. [Q8BPM0-3]
uc007nux.2. mouse. [Q8BPM0-1]
uc007nuz.2. mouse. [Q8BPM0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY426535 mRNA. Translation: AAR05118.1.
AK006902 mRNA. Translation: BAB24785.1.
AK018919 mRNA. Translation: BAB31482.1.
AK018950 mRNA. Translation: BAB31491.1.
AK053785 mRNA. Translation: BAC35522.1.
AK147480 mRNA. Translation: BAE27943.1.
BC032287 mRNA. No translation available.
BC048856 mRNA. Translation: AAH48856.1. Different initiation.
BC076585 mRNA. Translation: AAH76585.1.
CCDSiCCDS25964.1. [Q8BPM0-1]
RefSeqiNP_001273381.1. NM_001286452.1. [Q8BPM0-2]
NP_080378.2. NM_026102.3. [Q8BPM0-1]
NP_766052.2. NM_172464.3. [Q8BPM0-1]
XP_006515705.1. XM_006515642.2. [Q8BPM0-1]
XP_006515706.1. XM_006515643.2. [Q8BPM0-2]
XP_006515707.1. XM_006515644.2. [Q8BPM0-2]
UniGeneiMm.474935.
Mm.87417.

3D structure databases

ProteinModelPortaliQ8BPM0.
SMRiQ8BPM0. Positions 52-446, 601-1047.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8BPM0. 2 interactions.
STRINGi10090.ENSMUSP00000082406.

PTM databases

iPTMnetiQ8BPM0.
PhosphoSiteiQ8BPM0.
SwissPalmiQ8BPM0.

Proteomic databases

MaxQBiQ8BPM0.
PaxDbiQ8BPM0.
PeptideAtlasiQ8BPM0.
PRIDEiQ8BPM0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085299; ENSMUSP00000082406; ENSMUSG00000034574. [Q8BPM0-1]
GeneIDi208846.
KEGGimmu:208846.
UCSCiuc007nuw.2. mouse. [Q8BPM0-3]
uc007nux.2. mouse. [Q8BPM0-1]
uc007nuz.2. mouse. [Q8BPM0-2]

Organism-specific databases

CTDi23002.
MGIiMGI:1914596. Daam1.

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
ENOG410XT5Z. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000237318.
HOVERGENiHBG101333.
InParanoidiQ8BPM0.
KOiK04512.
OMAiWNQYSGR.
OrthoDBiEOG091G02QJ.
TreeFamiTF314602.

Enzyme and pathway databases

ReactomeiR-MMU-4086400. PCP/CE pathway.
R-MMU-5663220. RHO GTPases Activate Formins.

Miscellaneous databases

PROiQ8BPM0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034574.
GenevisibleiQ8BPM0. MM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027650. DAAM1.
IPR014767. DAD_dom.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
[Graphical view]
PANTHERiPTHR23213:SF181. PTHR23213:SF181. 2 hits.
PfamiPF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAAM1_MOUSE
AccessioniPrimary (citable) accession number: Q8BPM0
Secondary accession number(s): Q3UHB4
, Q6DFY0, Q6TAB8, Q80Y68, Q9CQQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 118 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.