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UniProtKB - Q8BPM0 (DAAM1_MOUSE)

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Protein

Disheveled-associated activator of morphogenesis 1

Gene

Daam1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity). Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionActin-binding

Enzyme and pathway databases

ReactomeiR-MMU-4086400. PCP/CE pathway.
R-MMU-5663220. RHO GTPases Activate Formins.

Names & Taxonomyi

Protein namesi
Recommended name:
Disheveled-associated activator of morphogenesis 1
Gene namesi
Name:Daam1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1914596. Daam1.

Subcellular locationi

  • Cytoplasm By similarity
  • Cytoplasm › cytoskeleton › cilium basal body By similarity

    • Note: Perinuclear.By similarity

GO - Cellular componenti

  • ciliary basal body Source: MGI
  • cytosol Source: MGI
  • membrane Source: MGI
  • motile cilium Source: MGI
  • plasma membrane Source: MGI
  • stress fiber Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949081 – 1077Disheveled-associated activator of morphogenesis 1Add BLAST1077

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34PhosphoserineBy similarity1
Modified residuei1026PhosphoserineBy similarity1
Modified residuei1029PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BPM0.
PeptideAtlasiQ8BPM0.
PRIDEiQ8BPM0.

PTM databases

iPTMnetiQ8BPM0.
PhosphoSitePlusiQ8BPM0.
SwissPalmiQ8BPM0.

Expressioni

Tissue specificityi

In early embryogenesis, expressed in embryonic and extraembryonic ectoderm. In later stages of gastrulation, expressed also in somites and ribs and posterior vertebrae of developing skeletal system. During organogenesis, expressed in CNS, PNS, stomach, liver and limb bud.2 Publications

Gene expression databases

BgeeiENSMUSG00000034574.
GenevisibleiQ8BPM0. MM.

Interactioni

Subunit structurei

Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA. Interacts with INTU; INTU mediates the indirect interaction between DAAM1 and NPHP4 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ARHGEF11O15085-23EBI-772938,EBI-6169263From a different organism.

GO - Molecular functioni

Complete GO annotation...

Protein-protein interaction databases

IntActiQ8BPM0. 2 interactors.
STRINGi10090.ENSMUSP00000082406.

Structurei

3D structure databases

ProteinModelPortaliQ8BPM0.
SMRiQ8BPM0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 420GBD/FH3PROSITE-ProRule annotationAdd BLAST376
Domaini528 – 599FH1Add BLAST72
Domaini600 – 1008FH2PROSITE-ProRule annotationAdd BLAST409
Domaini1026 – 1057DADPROSITE-ProRule annotationAdd BLAST32

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni693 – 702Actin-bindingBy similarity10

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili437 – 526Sequence analysisAdd BLAST90

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi528 – 593Pro-richAdd BLAST66

Domaini

The C-terminal DAD domain may participate in intramolecular interactions with the N-terminus.
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1922. Eukaryota.
ENOG410XT5Z. LUCA.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000237318.
HOVERGENiHBG101333.
InParanoidiQ8BPM0.
KOiK04512.
OMAiPLGGIMP.
OrthoDBiEOG091G02QJ.
TreeFamiTF314602.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027650. DAAM1.
IPR014767. DAD_dom.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR014768. GBD/FH3_dom.
IPR010473. GTPase-bd.
PANTHERiPTHR23213:SF292. PTHR23213:SF292. 1 hit.
PfamiView protein in Pfam
PF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
SMARTiView protein in SMART
SM01139. Drf_FH3. 1 hit.
SM01140. Drf_GBD. 1 hit.
SM00498. FH2. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiView protein in PROSITE
PS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BPM0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPRKRGGRG ISFIFCCFRN NDHPEITYRL RNDSNFALQT MEPALPMPPV 
        60         70         80         90        100
EELDVMFSEL VDELDLTDKH REAMFALPAE KKWQIYCSKK KDQEENKGAT 
       110        120        130        140        150
SWPEFYIDQL NSMAARKSLL ALEKEEEEER SKTIESLKTA LRTKPMRFVT 
       160        170        180        190        200
RFIDLDGLSC ILNFLKTMDY ETSESRIHTS LIGCIKALMN NSQGRAHVLA 
       210        220        230        240        250
HSESINVIAQ SLSTENIKTK VAVLEILGAV CLVPGGHKKV LQAMLHYQKY 
       260        270        280        290        300
ASERTRFQTL INDLDKSTGR YRDEVSLKTA IMSFINAVLS QGAGVESLDF 
       310        320        330        340        350
RLHLRYEFLM LGIQPVIDKL REHENSTLDR HLDFFEMLRN EDELEFAKRF 
       360        370        380        390        400
ELVHIDTKSA TQMFELTRRR LTHSEAYPHF MSILHHCLQM PYKRSGNTVQ 
       410        420        430        440        450
YWLLLDRIIQ QIVIQNDKGQ DPDSTPLENF NIKNVVRMLV NENEVKQWKE 
       460        470        480        490        500
QAEKMRKEHN ELQQKLEKKE RECDAKTQEK EEMMQTLNKM KEKLEKETTE 
       510        520        530        540        550
HKQVKQQVAD LTAQLHELNR RAVCAAVPGG PSPGAPGGPF PSSGLGSLLP 
       560        570        580        590        600
PPPPPLLSGG ALPPPPPPLP PGGPPPPPGP PPLGGVLPPP GAPVSLTLKK 
       610        620        630        640        650
KNIPQPTNAL KSFNWSKLPE NKLDGTVWTE IDDTKVFKIL DLEDLERTFS 
       660        670        680        690        700
AYQRQQEFFV NNSKQKEADA IDDTLSSKLK VKELSVIDGR RAQNCNILLS 
       710        720        730        740        750
RLKLSNDEIK RAILTMDEQE DLPKDMLEQL LKFVPEKSDI DLLEEHKHEL 
       760        770        780        790        800
DRMAKADRFL FEMSRINHYQ QRLQSLYFKK KFAERVAEVK PKVEAIRSGS 
       810        820        830        840        850
EEVFRSRALK QLLEVVLAFG NYMNKGQRGN AYGFKISSLN KIADTKSSID 
       860        870        880        890        900
KNITLLHYLI TIVENKYPKV LNLSEELRDI PQAAKVNMTE LDKEISTLRS 
       910        920        930        940        950
GLKAVETELE YQKSQPPQPG DKFVSVVSQF ITLASFSFSD VEDLLAEAKE 
       960        970        980        990       1000
LFTKAVKHFG EEAGKIQPDE FFGIFDQFLQ AVAEAKQENE NMRKRKEEEE 
      1010       1020       1030       1040       1050
RRARLEAQLK EQRERERKVR KAKESSEESG EFDDLVSALR SGEVFDKDLS 
      1060       1070 
KLKRNRKRIS NQVTDSSRER PITKLNF
Length:1,077
Mass (Da):123,370
Last modified:July 27, 2011 - v4
Checksum:iE16AC9DEF6E05B15
GO
Isoform 2 (identifier: Q8BPM0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     657-665: Missing.

Show »
Length:1,068
Mass (Da):122,276
Checksum:iF5AD716B8D158DD3
GO
Isoform 3 (identifier: Q8BPM0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     657-659: EFF → VTT
     660-1077: Missing.

Note: No experimental confirmation available.
Show »
Length:659
Mass (Da):74,953
Checksum:i59B031884A39FEA5
GO

Sequence cautioni

The sequence AAH48856 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36F → L in AAR05118 (PubMed:15533824).Curated1
Sequence conflicti571P → A in BAC35522 (PubMed:16141072).Curated1
Sequence conflicti894E → G in BAE27943 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027771657 – 665Missing in isoform 2. 2 Publications9
Alternative sequenceiVSP_027772657 – 659EFF → VTT in isoform 3. 1 Publication3
Alternative sequenceiVSP_027773660 – 1077Missing in isoform 3. 1 PublicationAdd BLAST418

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY426535 mRNA. Translation: AAR05118.1.
AK006902 mRNA. Translation: BAB24785.1.
AK018919 mRNA. Translation: BAB31482.1.
AK018950 mRNA. Translation: BAB31491.1.
AK053785 mRNA. Translation: BAC35522.1.
AK147480 mRNA. Translation: BAE27943.1.
BC032287 mRNA. No translation available.
BC048856 mRNA. Translation: AAH48856.1. Different initiation.
BC076585 mRNA. Translation: AAH76585.1.
CCDSiCCDS25964.1. [Q8BPM0-1]
RefSeqiNP_001273381.1. NM_001286452.1. [Q8BPM0-2]
NP_080378.2. NM_026102.3. [Q8BPM0-1]
NP_766052.2. NM_172464.3. [Q8BPM0-1]
XP_006515705.1. XM_006515642.2. [Q8BPM0-1]
XP_006515706.1. XM_006515643.3. [Q8BPM0-2]
XP_006515707.1. XM_006515644.3. [Q8BPM0-2]
UniGeneiMm.474935.
Mm.87417.

Genome annotation databases

EnsembliENSMUST00000085299; ENSMUSP00000082406; ENSMUSG00000034574. [Q8BPM0-1]
GeneIDi208846.
KEGGimmu:208846.
UCSCiuc007nuw.2. mouse. [Q8BPM0-3]
uc007nux.2. mouse. [Q8BPM0-1]
uc007nuz.2. mouse. [Q8BPM0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDAAM1_MOUSE
AccessioniPrimary (citable) accession number: Q8BPM0
Secondary accession number(s): Q3UHB4
, Q6DFY0, Q6TAB8, Q80Y68, Q9CQQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: July 27, 2011
Last modified: June 7, 2017
This is version 126 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families