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Q8BPI1 (KTU_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein kintoun
Alternative name(s):
Dynein assembly factor 2, axonemal
Gene names
Name:Dnaaf2
Synonyms:Ktu
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length814 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for cytoplasmic pre-assembly of axonemal dyneins, thereby playing a central role in motility in cilia and flagella. Involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment By similarity.

Subunit structure

Interacts with DNAI2 and HSPA1A. Interacts with DYX1C1 By similarity. Ref.1

Subcellular location

Cytoplasm By similarity. Note: Localizes in the apical cytoplasm around the gamma-tubulin-positive pericentriolar region, not in the cilia By similarity.

Tissue specificity

Expressed in nearly all organs of adult, with higher expression in tissues known to have motile cilia and flagella, such as brain and testis. Ref.1

Sequence similarities

Belongs to the PIH1 family. Kintoun subfamily.

Sequence caution

The sequence AAH58344.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAB28455.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAC35879.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAE32466.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence BAE43032.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BPI1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BPI1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     400-437: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 814814Protein kintoun
PRO_0000089912

Amino acid modifications

Modified residue4441Phosphoserine By similarity
Modified residue6181Phosphoserine By similarity

Natural variations

Alternative sequence400 – 43738Missing in isoform 2.
VSP_036538

Experimental info

Sequence conflict4631L → Q in BAB28455. Ref.3
Sequence conflict7531S → T in BAE32466. Ref.3
Sequence conflict7911V → E in BAB28455. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 3, 2009. Version 2.
Checksum: 62F63A64B3BE73E3

FASTA81488,327
        10         20         30         40         50         60 
MAKAAASSAL EDLDLSREEV QRFTSAFQDP EFRRMFSEYA AEITDPENRR RYEEEITALE 

        70         80         90        100        110        120 
RERGVDVRFV HPEPGHVLRT SLDGEHRCYV NVCSNSLVGV PSSRPGPGRG GTAAGSHWSL 

       130        140        150        160        170        180 
PYSLAPGRQY AGRNGARYTV YDVVFHPEAL ALARSHERFR EMLDATALEA VEQQFGVRLD 

       190        200        210        220        230        240 
RRNAKTLKIK YKGMPEAAVL RTPLPGGVPA QPEGEPPGLF PDPPYPYRYP AAAAANTARS 

       250        260        270        280        290        300 
PASPAPEAVQ RPEPTEPRCS VVQRHHVDLQ DYRCSRDAAP STVPHELVVT IELPLLRSVE 

       310        320        330        340        350        360 
RAELEVKGKL LCLDSRNPDY RLRLSLPYPV DDGRGKAQYN KARRQLVVTL PVALADARQE 

       370        380        390        400        410        420 
PPAATPEEPA EETGTDDVAR TSAGDFAAAR EESADGTGAD HGEKSGVGAP DPGAAHAEGE 

       430        440        450        460        470        480 
LVPEPEQDFG GDSVAPLDLG KGTSPGDRSL PYSAFPGGDT ESLCGDPGVQ TNEEQERTRH 

       490        500        510        520        530        540 
DTAGSAMGDP GTESIAPVCP PLQCNQDEDS LTLLIQVPGI LPQTLHGHLS PVGYELCFST 

       550        560        570        580        590        600 
QDSGYSCTLQ FAPENKLSTR EPETSVSLNN AVIVLAKSPE SHGLWREWYC GLNKESLEER 

       610        620        630        640        650        660 
LFINEENVNG FLEEVLCSPL KQARSLAPPL IEVLQATDEQ VQIHAELQEC SDPAGLQGKG 

       670        680        690        700        710        720 
KGVREGCPLS EAEAADQSAT SPAASDSAAA VEALKINTHG SAVDLQHGCP EVPHVLSGKP 

       730        740        750        760        770        780 
LQPEAKMDPE FIRESSTTYS TEEKENIREP VISKGEKING DHPSSLLNKT VVQNIPDFDT 

       790        800        810 
IKETNMQDGS VQIIRDHTTH CAFSFQNPLL YDLD 

« Hide

Isoform 2 [UniParc].

Checksum: CA6EA380AF386A6F
Show »

FASTA77684,605

References

« Hide 'large scale' references
[1]"Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins."
Omran H., Kobayashi D., Olbrich H., Tsukahara T., Loges N.T., Hagiwara H., Zhang Q., Leblond G., O'Toole E., Hara C., Mizuno H., Kawano H., Fliegauf M., Yagi T., Koshida S., Miyawaki A., Zentgraf H., Seithe H. expand/collapse author list , Reinhardt R., Watanabe Y., Kamiya R., Mitchell D.R., Takeda H.
Nature 456:611-616(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, INTERACTION WITH DNAI2 AND HSPA1A.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 387-814 (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 385-814 (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Embryo and Spleen.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB455811 mRNA. Translation: BAG75151.1.
BC055807 mRNA. Translation: AAH55807.1.
BC058344 mRNA. Translation: AAH58344.1. Different initiation.
BC062898 mRNA. Translation: AAH62898.1.
AK012767 mRNA. Translation: BAB28455.1. Different initiation.
AK075648 mRNA. Translation: BAC35879.1. Different initiation.
AK154254 mRNA. Translation: BAE32466.1. Different initiation.
AK172494 mRNA. Translation: BAE43032.1. Different initiation.
CCDSCCDS25948.1. [Q8BPI1-1]
RefSeqNP_081545.3. NM_027269.3. [Q8BPI1-1]
UniGeneMm.107180.

3D structure databases

ProteinModelPortalQ8BPI1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59777N.

PTM databases

PhosphoSiteQ8BPI1.

Proteomic databases

PaxDbQ8BPI1.
PRIDEQ8BPI1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021356; ENSMUSP00000021356; ENSMUSG00000020973. [Q8BPI1-1]
GeneID109065.
KEGGmmu:109065.
UCSCuc007nrt.1. mouse. [Q8BPI1-1]

Organism-specific databases

CTD55172.
MGIMGI:1923566. Dnaaf2.

Phylogenomic databases

eggNOGNOG40282.
GeneTreeENSGT00510000048466.
HOGENOMHOG000094570.
HOVERGENHBG108080.
InParanoidQ3U4G5.
OMACLDSRKP.
OrthoDBEOG7CRTP9.
TreeFamTF336215.

Gene expression databases

BgeeQ8BPI1.
CleanExMM_1110034A24RIK.
GenevestigatorQ8BPI1.

Family and domain databases

InterProIPR012981. PIH1.
[Graphical view]
PANTHERPTHR22997. PTHR22997. 1 hit.
PfamPF08190. PIH1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio361616.
PROQ8BPI1.
SOURCESearch...

Entry information

Entry nameKTU_MOUSE
AccessionPrimary (citable) accession number: Q8BPI1
Secondary accession number(s): Q3T9I8 expand/collapse secondary AC list , Q3U4G5, Q6P5G9, Q9CZC7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: March 3, 2009
Last modified: July 9, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot