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Protein

Protein kintoun

Gene

Dnaaf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for cytoplasmic pre-assembly of axonemal dyneins, thereby playing a central role in motility in cilia and flagella. Involved in pre-assembly of dynein arm complexes in the cytoplasm before intraflagellar transport loads them for the ciliary compartment.UniRule annotation

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kintounUniRule annotation
Alternative name(s):
Dynein assembly factor 2, axonemalUniRule annotation
Gene namesi
Name:Dnaaf2UniRule annotation
Synonyms:KtuUniRule annotation
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1923566. Dnaaf2.

Subcellular locationi

  • Cytoplasm UniRule annotation

  • Note: Localizes in the apical cytoplasm around the gamma-tubulin-positive pericentriolar region, not in the cilia.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000899121 – 814Protein kintounAdd BLAST814

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei444PhosphoserineBy similarity1
Modified residuei618PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8BPI1.
PRIDEiQ8BPI1.

PTM databases

iPTMnetiQ8BPI1.
PhosphoSitePlusiQ8BPI1.

Expressioni

Tissue specificityi

Expressed in nearly all organs of adult, with higher expression in tissues known to have motile cilia and flagella, such as brain and testis.1 Publication

Gene expression databases

BgeeiENSMUSG00000020973.
CleanExiMM_1110034A24RIK.
GenevisibleiQ8BPI1. MM.

Interactioni

Subunit structurei

Interacts with DNAI2 and HSPA1A. Interacts with DYX1C1.UniRule annotation

Protein-protein interaction databases

DIPiDIP-59777N.
STRINGi10090.ENSMUSP00000021356.

Structurei

3D structure databases

ProteinModelPortaliQ8BPI1.
SMRiQ8BPI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIH1 family. Kintoun subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG4356. Eukaryota.
ENOG410XQ8C. LUCA.
GeneTreeiENSGT00510000048466.
HOGENOMiHOG000094570.
HOVERGENiHBG108080.
InParanoidiQ8BPI1.
KOiK19751.
OMAiPDFPYPY.
OrthoDBiEOG091G0J98.
TreeFamiTF336215.

Family and domain databases

HAMAPiMF_03069. Kintoun. 1 hit.
InterProiIPR012981. PIH1.
[Graphical view]
PANTHERiPTHR22997. PTHR22997. 2 hits.
PfamiPF08190. PIH1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BPI1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKAAASSAL EDLDLSREEV QRFTSAFQDP EFRRMFSEYA AEITDPENRR
60 70 80 90 100
RYEEEITALE RERGVDVRFV HPEPGHVLRT SLDGEHRCYV NVCSNSLVGV
110 120 130 140 150
PSSRPGPGRG GTAAGSHWSL PYSLAPGRQY AGRNGARYTV YDVVFHPEAL
160 170 180 190 200
ALARSHERFR EMLDATALEA VEQQFGVRLD RRNAKTLKIK YKGMPEAAVL
210 220 230 240 250
RTPLPGGVPA QPEGEPPGLF PDPPYPYRYP AAAAANTARS PASPAPEAVQ
260 270 280 290 300
RPEPTEPRCS VVQRHHVDLQ DYRCSRDAAP STVPHELVVT IELPLLRSVE
310 320 330 340 350
RAELEVKGKL LCLDSRNPDY RLRLSLPYPV DDGRGKAQYN KARRQLVVTL
360 370 380 390 400
PVALADARQE PPAATPEEPA EETGTDDVAR TSAGDFAAAR EESADGTGAD
410 420 430 440 450
HGEKSGVGAP DPGAAHAEGE LVPEPEQDFG GDSVAPLDLG KGTSPGDRSL
460 470 480 490 500
PYSAFPGGDT ESLCGDPGVQ TNEEQERTRH DTAGSAMGDP GTESIAPVCP
510 520 530 540 550
PLQCNQDEDS LTLLIQVPGI LPQTLHGHLS PVGYELCFST QDSGYSCTLQ
560 570 580 590 600
FAPENKLSTR EPETSVSLNN AVIVLAKSPE SHGLWREWYC GLNKESLEER
610 620 630 640 650
LFINEENVNG FLEEVLCSPL KQARSLAPPL IEVLQATDEQ VQIHAELQEC
660 670 680 690 700
SDPAGLQGKG KGVREGCPLS EAEAADQSAT SPAASDSAAA VEALKINTHG
710 720 730 740 750
SAVDLQHGCP EVPHVLSGKP LQPEAKMDPE FIRESSTTYS TEEKENIREP
760 770 780 790 800
VISKGEKING DHPSSLLNKT VVQNIPDFDT IKETNMQDGS VQIIRDHTTH
810
CAFSFQNPLL YDLD
Length:814
Mass (Da):88,327
Last modified:March 3, 2009 - v2
Checksum:i62F63A64B3BE73E3
GO
Isoform 2 (identifier: Q8BPI1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     400-437: Missing.

Note: No experimental confirmation available.
Show »
Length:776
Mass (Da):84,605
Checksum:iCA6EA380AF386A6F
GO

Sequence cautioni

The sequence AAH58344 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB28455 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC35879 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE32466 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE43032 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti463L → Q in BAB28455 (PubMed:16141072).Curated1
Sequence conflicti753S → T in BAE32466 (PubMed:16141072).Curated1
Sequence conflicti791V → E in BAB28455 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036538400 – 437Missing in isoform 2. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB455811 mRNA. Translation: BAG75151.1.
BC055807 mRNA. Translation: AAH55807.1.
BC058344 mRNA. Translation: AAH58344.1. Different initiation.
BC062898 mRNA. Translation: AAH62898.1.
AK012767 mRNA. Translation: BAB28455.1. Different initiation.
AK075648 mRNA. Translation: BAC35879.1. Different initiation.
AK154254 mRNA. Translation: BAE32466.1. Different initiation.
AK172494 mRNA. Translation: BAE43032.1. Different initiation.
CCDSiCCDS25948.1. [Q8BPI1-1]
RefSeqiNP_081545.3. NM_027269.4. [Q8BPI1-1]
UniGeneiMm.107180.

Genome annotation databases

EnsembliENSMUST00000021356; ENSMUSP00000021356; ENSMUSG00000020973. [Q8BPI1-1]
GeneIDi109065.
KEGGimmu:109065.
UCSCiuc007nrt.1. mouse. [Q8BPI1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB455811 mRNA. Translation: BAG75151.1.
BC055807 mRNA. Translation: AAH55807.1.
BC058344 mRNA. Translation: AAH58344.1. Different initiation.
BC062898 mRNA. Translation: AAH62898.1.
AK012767 mRNA. Translation: BAB28455.1. Different initiation.
AK075648 mRNA. Translation: BAC35879.1. Different initiation.
AK154254 mRNA. Translation: BAE32466.1. Different initiation.
AK172494 mRNA. Translation: BAE43032.1. Different initiation.
CCDSiCCDS25948.1. [Q8BPI1-1]
RefSeqiNP_081545.3. NM_027269.4. [Q8BPI1-1]
UniGeneiMm.107180.

3D structure databases

ProteinModelPortaliQ8BPI1.
SMRiQ8BPI1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59777N.
STRINGi10090.ENSMUSP00000021356.

PTM databases

iPTMnetiQ8BPI1.
PhosphoSitePlusiQ8BPI1.

Proteomic databases

PaxDbiQ8BPI1.
PRIDEiQ8BPI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021356; ENSMUSP00000021356; ENSMUSG00000020973. [Q8BPI1-1]
GeneIDi109065.
KEGGimmu:109065.
UCSCiuc007nrt.1. mouse. [Q8BPI1-1]

Organism-specific databases

CTDi55172.
MGIiMGI:1923566. Dnaaf2.

Phylogenomic databases

eggNOGiKOG4356. Eukaryota.
ENOG410XQ8C. LUCA.
GeneTreeiENSGT00510000048466.
HOGENOMiHOG000094570.
HOVERGENiHBG108080.
InParanoidiQ8BPI1.
KOiK19751.
OMAiPDFPYPY.
OrthoDBiEOG091G0J98.
TreeFamiTF336215.

Miscellaneous databases

PROiQ8BPI1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020973.
CleanExiMM_1110034A24RIK.
GenevisibleiQ8BPI1. MM.

Family and domain databases

HAMAPiMF_03069. Kintoun. 1 hit.
InterProiIPR012981. PIH1.
[Graphical view]
PANTHERiPTHR22997. PTHR22997. 2 hits.
PfamiPF08190. PIH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKTU_MOUSE
AccessioniPrimary (citable) accession number: Q8BPI1
Secondary accession number(s): Q3T9I8
, Q3U4G5, Q6P5G9, Q9CZC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: March 3, 2009
Last modified: November 30, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.