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Protein

RNA binding protein fox-1 homolog 2

Gene

Rbfox2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. Seems to act as a coregulatory factor of ER-alpha (By similarity).By similarity6 Publications

GO - Molecular functioni

GO - Biological processi

  • dendrite morphogenesis Source: MGI
  • intracellular estrogen receptor signaling pathway Source: UniProtKB
  • mRNA processing Source: UniProtKB-KW
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • neuromuscular process controlling balance Source: MGI
  • radial glia guided migration of Purkinje cell Source: MGI
  • regulation of alternative mRNA splicing, via spliceosome Source: BHF-UCL
  • regulation of definitive erythrocyte differentiation Source: BHF-UCL
  • RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA binding protein fox-1 homolog 2
Alternative name(s):
Fox-1 homolog B
Fox-1 homolog Fxh
Hexaribonucleotide-binding protein 2
RNA-binding motif protein 9
RNA-binding protein 9
Gene namesi
Name:Rbfox2
Synonyms:Fox2, Fxh, Hrnbp2, Rbm9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1933973. Rbfox2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003171151 – 449RNA binding protein fox-1 homolog 2Add BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei340Omega-N-methylarginineCombined sources1
Modified residuei356Asymmetric dimethylarginineCombined sources1
Modified residuei388Asymmetric dimethylarginineCombined sources1
Modified residuei440Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei440Omega-N-methylarginine; alternateCombined sources1
Modified residuei445Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei445Omega-N-methylarginine; alternateCombined sources1
Isoform 4 (identifier: Q8BP71-4)
Modified residuei236Omega-N-methylarginineCombined sources1
Modified residuei241Omega-N-methylarginineCombined sources1
Modified residuei285Asymmetric dimethylarginineCombined sources1
Isoform 6 (identifier: Q8BP71-6)
Modified residuei268Omega-N-methylarginineCombined sources1
Modified residuei273Omega-N-methylarginineCombined sources1
Isoform 7 (identifier: Q8BP71-7)
Modified residuei268Omega-N-methylarginineCombined sources1
Modified residuei273Omega-N-methylarginineCombined sources1
Isoform 8 (identifier: Q8BP71-8)
Modified residuei268Omega-N-methylarginineCombined sources1
Modified residuei273Omega-N-methylarginineCombined sources1
Modified residuei317Asymmetric dimethylarginineCombined sources1
Isoform 2 (identifier: Q8BP71-2)
Modified residuei318Omega-N-methylarginineCombined sources1
Modified residuei323Omega-N-methylarginineCombined sources1
Isoform 5 (identifier: Q8BP71-5)
Modified residuei336Omega-N-methylarginineCombined sources1
Modified residuei341Omega-N-methylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ8BP71.
PeptideAtlasiQ8BP71.
PRIDEiQ8BP71.

PTM databases

iPTMnetiQ8BP71.
PhosphoSitePlusiQ8BP71.

Expressioni

Tissue specificityi

Detected in brain neurons (at protein level). Detected in heart, brain, embryo, lung, liver, kidney and ovary.2 Publications

Inductioni

Up-regulated by androgens in cultured motor neuron cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000033565.
CleanExiMM_RBM9.
GenevisibleiQ8BP71. MM.

Interactioni

Subunit structurei

Interacts with ER-alpha N-terminal activation domain.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei181Interaction with RNABy similarity1
Sitei189Interaction with RNABy similarity1
Sitei190Interaction with RNABy similarity1
Sitei214Interaction with RNABy similarity1
Sitei219Interaction with RNABy similarity1
Sitei223Interaction with RNABy similarity1
Sitei247Interaction with RNABy similarity1
Sitei257Interaction with RNABy similarity1

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220236. 4 interactors.
IntActiQ8BP71. 1 interactor.
STRINGi10090.ENSMUSP00000130739.

Structurei

3D structure databases

ProteinModelPortaliQ8BP71.
SMRiQ8BP71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 256RRMPROSITE-ProRule annotationAdd BLAST77

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi332 – 436Ala-richAdd BLAST105

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0125. Eukaryota.
ENOG4111WJT. LUCA.
GeneTreeiENSGT00390000013767.
HOVERGENiHBG000044.
InParanoidiQ8BP71.
KOiK14946.
OMAiPRNPCEN.
OrthoDBiEOG091G15X5.
PhylomeDBiQ8BP71.
TreeFamiTF315942.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR025670. Fox-1_C_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR017325. RNA-bd_Fox-1.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF12414. Fox-1_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037932. Ataxin_2_bd_A2BP. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (9)i

Sequence statusi: Complete.

This entry describes 9 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BP71-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEGGQAQQQ PPQLGPGAAA RGMKRESEVE LPVPGAGADG PEPGLSKRPR
60 70 80 90 100
TEEAADGGMQ NEPLTPGYHG FPARDGQGNQ EPTTTPDAMV QPFTTIPFPP
110 120 130 140 150
PPQNGIPTEY GVPHTQDYAG QTSEHNLTLY GSTQPHGEQS SNSPSNQNGS
160 170 180 190 200
LTQTEGGAQT DGQQSQTQSS ENSESKSTPK RLHVSNIPFR FRDPDLRQMF
210 220 230 240 250
GQFGKILDVE IIFNERGSKG FGFVTFENSA DADRAREKLH GTVVEGRKIE
260 270 280 290 300
VNNATARVMT NKKMVTPYAN GWKLSPVVGA VYGPELYAAS SFQADVSLGN
310 320 330 340 350
EAAVPLSGRG GINTYIPLIS LPLVPGFPYP TAATTAAAFR GAHLRGRGRT
360 370 380 390 400
VYGAVRAVPP TAIPAYPGVV YQDGFYGADL YGGYAAYRYA QPATATAATA
410 420 430 440
AAAAAAAYSD GYGRVYTADP YHALAPAASY GVGAVASLYR GGYSRFAPY
Length:449
Mass (Da):47,330
Last modified:February 5, 2008 - v2
Checksum:iC4B01F814B3C8F5A
GO
Isoform 2 (identifier: Q8BP71-2) [UniParc]FASTAAdd to basket
Also known as: Fox-2A

The sequence of this isoform differs from the canonical sequence as follows:
     19-36: Missing.
     320-324: SLPLV → I

Show »
Length:427
Mass (Da):45,055
Checksum:i31C4B28B1EE6E808
GO
Isoform 3 (identifier: Q8BP71-3) [UniParc]FASTAAdd to basket
Also known as: 1, Fox-2F

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: MAEGGQAQQQ...GYHGFPARDG → MEKKKMVT

Show »
Length:381
Mass (Da):40,562
Checksum:iC75B29D5F650FE29
GO
Isoform 4 (identifier: Q8BP71-4) [UniParc]FASTAAdd to basket
Also known as: 2, Fox-2F-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: MAEGGQAQQQ...GYHGFPARDG → MEKKKMVT
     153-153: Missing.
     220-250: Missing.
     320-324: SLPLV → I
     369-381: VVYQDGFYGADLY → IVLQEPIISAKIPQ

Show »
Length:346
Mass (Da):36,658
Checksum:i8E96304EC3706B0C
GO
Isoform 5 (identifier: Q8BP71-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     320-324: SLPLV → I
     370-449: VYQDGFYGAD...RGGYSRFAPY → DMQPTDMHSL...TADLPPTEVT

Show »
Length:435
Mass (Da):46,253
Checksum:i62A355605FF8A5B4
GO
Isoform 6 (identifier: Q8BP71-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: MAEGGQAQQQ...GYHGFPARDG → MEKKKMVT
     320-324: SLPLV → I
     373-449: DGFYGADLYG...RGGYSRFAPY → DMQPTDMHSL...PLPPAMELAL

Show »
Length:352
Mass (Da):37,905
Checksum:i74F46B287F6C5A38
GO
Isoform 7 (identifier: Q8BP71-7) [UniParc]FASTAAdd to basket
Also known as: 1, F011

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: MAEGGQAQQQ...GYHGFPARDG → MEKKKMVT
     320-324: SLPLV → I

Show »
Length:377
Mass (Da):40,166
Checksum:i249FA9BA6A0D155A
GO
Isoform 8 (identifier: Q8BP71-8) [UniParc]FASTAAdd to basket
Also known as: 2, F411

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: MAEGGQAQQQ...GYHGFPARDG → MEKKKMVT
     320-324: SLPLV → I
     369-381: VVYQDGFYGADLY → IVLQEPIISAKIPQ

Show »
Length:378
Mass (Da):40,205
Checksum:iF1CB87822F3EC257
GO
Isoform 9 (identifier: Q8BP71-9) [UniParc]FASTAAdd to basket
Also known as: 3, F402

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: MAEGGQAQQQ...GYHGFPARDG → MEKKKMVT
     153-250: Missing.

Show »
Length:283
Mass (Da):29,576
Checksum:i6CA06BED5F616238
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88A → G in BAC32147 (PubMed:16141072).Curated1
Sequence conflicti306L → M in AAL71902 (PubMed:16537540).Curated1
Sequence conflicti342A → D in BAC36885 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0308921 – 76MAEGG…PARDG → MEKKKMVT in isoform 3, isoform 4, isoform 6, isoform 7, isoform 8 and isoform 9. 6 PublicationsAdd BLAST76
Alternative sequenceiVSP_03089319 – 36Missing in isoform 2. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_030894153 – 250Missing in isoform 9. 2 PublicationsAdd BLAST98
Alternative sequenceiVSP_030895153Missing in isoform 4. 1 Publication1
Alternative sequenceiVSP_030896220 – 250Missing in isoform 4. 1 PublicationAdd BLAST31
Alternative sequenceiVSP_030897320 – 324SLPLV → I in isoform 2, isoform 4, isoform 5, isoform 6, isoform 7 and isoform 8. 6 Publications5
Alternative sequenceiVSP_030898369 – 381VVYQD…GADLY → IVLQEPIISAKIPQ in isoform 4 and isoform 8. 2 PublicationsAdd BLAST13
Alternative sequenceiVSP_030899370 – 449VYQDG…RFAPY → DMQPTDMHSLLLQPQPQLLQ PLQPLTATVTAGCTQLTPTM PSPLPPAMELALWRVCTEVA TADLPPTEVT in isoform 5. 1 PublicationAdd BLAST80
Alternative sequenceiVSP_030900373 – 449DGFYG…RFAPY → DMQPTDMHSLLLQPQPQLLQ PLQPLTATVTAGCTQLTPTM PSPLPPAMELAL in isoform 6. 1 PublicationAdd BLAST77

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF387322 mRNA. Translation: AAK64287.1.
AY659951 mRNA. Translation: AAV74334.1.
AY659952 mRNA. Translation: AAV74335.1.
AY659953 mRNA. Translation: AAV74336.1.
AF229055 mRNA. Translation: AAL71902.1.
AY904025 mRNA. Translation: AAX84842.1.
DQ017388 mRNA. Translation: AAY78494.1.
DQ017389 mRNA. Translation: AAY78495.1.
AK044929 mRNA. Translation: BAC32147.1.
AK077594 mRNA. Translation: BAC36885.1.
BC002124 mRNA. Translation: AAH02124.1.
BC027263 mRNA. Translation: AAH27263.1.
CCDSiCCDS27598.1. [Q8BP71-5]
CCDS49657.1. [Q8BP71-8]
CCDS49658.1. [Q8BP71-3]
CCDS49659.1. [Q8BP71-1]
RefSeqiNP_001104297.1. NM_001110827.2.
NP_001104298.1. NM_001110828.2. [Q8BP71-3]
NP_001104299.1. NM_001110829.2. [Q8BP71-8]
NP_001104300.1. NM_001110830.2. [Q8BP71-7]
NP_001273346.1. NM_001286417.1.
NP_001273347.1. NM_001286418.1. [Q8BP71-9]
NP_001273348.1. NM_001286419.1. [Q8BP71-4]
NP_444334.3. NM_053104.6. [Q8BP71-1]
NP_780596.1. NM_175387.3. [Q8BP71-5]
UniGeneiMm.202774.

Genome annotation databases

EnsembliENSMUST00000048145; ENSMUSP00000048056; ENSMUSG00000033565. [Q8BP71-5]
ENSMUST00000111581; ENSMUSP00000129372; ENSMUSG00000033565. [Q8BP71-8]
ENSMUST00000166610; ENSMUSP00000130673; ENSMUSG00000033565. [Q8BP71-3]
ENSMUST00000171751; ENSMUSP00000130739; ENSMUSG00000033565. [Q8BP71-1]
GeneIDi93686.
KEGGimmu:93686.
UCSCiuc007wnc.3. mouse. [Q8BP71-5]
uc007wnd.2. mouse. [Q8BP71-7]
uc007wne.2. mouse. [Q8BP71-8]
uc007wnf.2. mouse. [Q8BP71-4]
uc007wng.2. mouse. [Q8BP71-1]
uc007wni.2. mouse. [Q8BP71-2]
uc011zvf.1. mouse. [Q8BP71-3]
uc011zvg.2. mouse. [Q8BP71-9]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF387322 mRNA. Translation: AAK64287.1.
AY659951 mRNA. Translation: AAV74334.1.
AY659952 mRNA. Translation: AAV74335.1.
AY659953 mRNA. Translation: AAV74336.1.
AF229055 mRNA. Translation: AAL71902.1.
AY904025 mRNA. Translation: AAX84842.1.
DQ017388 mRNA. Translation: AAY78494.1.
DQ017389 mRNA. Translation: AAY78495.1.
AK044929 mRNA. Translation: BAC32147.1.
AK077594 mRNA. Translation: BAC36885.1.
BC002124 mRNA. Translation: AAH02124.1.
BC027263 mRNA. Translation: AAH27263.1.
CCDSiCCDS27598.1. [Q8BP71-5]
CCDS49657.1. [Q8BP71-8]
CCDS49658.1. [Q8BP71-3]
CCDS49659.1. [Q8BP71-1]
RefSeqiNP_001104297.1. NM_001110827.2.
NP_001104298.1. NM_001110828.2. [Q8BP71-3]
NP_001104299.1. NM_001110829.2. [Q8BP71-8]
NP_001104300.1. NM_001110830.2. [Q8BP71-7]
NP_001273346.1. NM_001286417.1.
NP_001273347.1. NM_001286418.1. [Q8BP71-9]
NP_001273348.1. NM_001286419.1. [Q8BP71-4]
NP_444334.3. NM_053104.6. [Q8BP71-1]
NP_780596.1. NM_175387.3. [Q8BP71-5]
UniGeneiMm.202774.

3D structure databases

ProteinModelPortaliQ8BP71.
SMRiQ8BP71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220236. 4 interactors.
IntActiQ8BP71. 1 interactor.
STRINGi10090.ENSMUSP00000130739.

PTM databases

iPTMnetiQ8BP71.
PhosphoSitePlusiQ8BP71.

Proteomic databases

PaxDbiQ8BP71.
PeptideAtlasiQ8BP71.
PRIDEiQ8BP71.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048145; ENSMUSP00000048056; ENSMUSG00000033565. [Q8BP71-5]
ENSMUST00000111581; ENSMUSP00000129372; ENSMUSG00000033565. [Q8BP71-8]
ENSMUST00000166610; ENSMUSP00000130673; ENSMUSG00000033565. [Q8BP71-3]
ENSMUST00000171751; ENSMUSP00000130739; ENSMUSG00000033565. [Q8BP71-1]
GeneIDi93686.
KEGGimmu:93686.
UCSCiuc007wnc.3. mouse. [Q8BP71-5]
uc007wnd.2. mouse. [Q8BP71-7]
uc007wne.2. mouse. [Q8BP71-8]
uc007wnf.2. mouse. [Q8BP71-4]
uc007wng.2. mouse. [Q8BP71-1]
uc007wni.2. mouse. [Q8BP71-2]
uc011zvf.1. mouse. [Q8BP71-3]
uc011zvg.2. mouse. [Q8BP71-9]

Organism-specific databases

CTDi23543.
MGIiMGI:1933973. Rbfox2.

Phylogenomic databases

eggNOGiKOG0125. Eukaryota.
ENOG4111WJT. LUCA.
GeneTreeiENSGT00390000013767.
HOVERGENiHBG000044.
InParanoidiQ8BP71.
KOiK14946.
OMAiPRNPCEN.
OrthoDBiEOG091G15X5.
PhylomeDBiQ8BP71.
TreeFamiTF315942.

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.

Miscellaneous databases

ChiTaRSiRbfox2. mouse.
PROiQ8BP71.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033565.
CleanExiMM_RBM9.
GenevisibleiQ8BP71. MM.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR025670. Fox-1_C_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR017325. RNA-bd_Fox-1.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF12414. Fox-1_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF037932. Ataxin_2_bd_A2BP. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRFOX2_MOUSE
AccessioniPrimary (citable) accession number: Q8BP71
Secondary accession number(s): Q0R5Z7
, Q0R5Z8, Q0VH84, Q537D4, Q537D5, Q8BRG0, Q8R2T5, Q8VI62, Q923W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: November 2, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.