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Protein

Neuronal calcium sensor 1

Gene

Ncs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi73 – 84121Add
BLAST
Calcium bindingi109 – 120122Add
BLAST
Calcium bindingi157 – 168123Add
BLAST

GO - Molecular functioni

  • calcium ion binding Source: MGI
  • magnesium ion binding Source: Ensembl
  • voltage-gated calcium channel activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal calcium sensor 1
Short name:
NCS-1
Alternative name(s):
Frequenin homolog
Gene namesi
Name:Ncs1
Synonyms:Freq
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:109166. Ncs1.

Subcellular locationi

  • Golgi apparatus By similarity
  • Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity
  • Cytoplasmperinuclear region By similarity
  • Cytoplasm By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Membrane By similarity; Lipid-anchor By similarity

  • Note: Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 190189Neuronal calcium sensor 1PRO_0000073789Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

MaxQBiQ8BNY6.
PaxDbiQ8BNY6.
PeptideAtlasiQ8BNY6.
PRIDEiQ8BNY6.

PTM databases

iPTMnetiQ8BNY6.
PhosphoSiteiQ8BNY6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000062661.
CleanExiMM_FREQ.
ExpressionAtlasiQ8BNY6. baseline and differential.
GenevisibleiQ8BNY6. MM.

Interactioni

Subunit structurei

Interacts with KCND2, ARF1, ARF3, ARF5 and ARF6 (By similarity). Interacts in a calcium-independent manner with PI4KB. This binding competes with CALN2/CABP7 binding to PI4KB (By similarity). Interacts in a calcium-dependent manner with PICK1 (via AH domain) (By similarity). Interacts with IL1RAPL1 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-61316N.
IntActiQ8BNY6. 2 interactions.
MINTiMINT-4439526.
STRINGi10090.ENSMUSP00000000199.

Structurei

3D structure databases

ProteinModelPortaliQ8BNY6.
SMRiQ8BNY6. Positions 3-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 5936EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini60 – 9536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini96 – 13136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini144 – 17936EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni174 – 19017Interaction with IL1RAPL1By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ8BNY6.
KOiK19932.
OMAiFHEFIRA.
OrthoDBiEOG091G11T4.
PhylomeDBiQ8BNY6.
TreeFamiTF300009.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BNY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK
60 70 80 90 100
IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK
110 120 130 140 150
LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD
160 170 180 190
RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV
Length:190
Mass (Da):21,879
Last modified:January 23, 2007 - v3
Checksum:i9AF8E26A23F80D4F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631K → E in BAC37557 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020184 mRNA. Translation: AAD01642.1.
AL732572 Genomic DNA. Translation: CAM21076.1.
BC059825 mRNA. Translation: AAH59825.1.
AK079136 mRNA. Translation: BAC37557.1.
CCDSiCCDS15896.1.
RefSeqiNP_062655.1. NM_019681.3.
UniGeneiMm.249318.

Genome annotation databases

EnsembliENSMUST00000000199; ENSMUSP00000000199; ENSMUSG00000062661.
GeneIDi14299.
KEGGimmu:14299.
UCSCiuc008jdq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF020184 mRNA. Translation: AAD01642.1.
AL732572 Genomic DNA. Translation: CAM21076.1.
BC059825 mRNA. Translation: AAH59825.1.
AK079136 mRNA. Translation: BAC37557.1.
CCDSiCCDS15896.1.
RefSeqiNP_062655.1. NM_019681.3.
UniGeneiMm.249318.

3D structure databases

ProteinModelPortaliQ8BNY6.
SMRiQ8BNY6. Positions 3-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61316N.
IntActiQ8BNY6. 2 interactions.
MINTiMINT-4439526.
STRINGi10090.ENSMUSP00000000199.

PTM databases

iPTMnetiQ8BNY6.
PhosphoSiteiQ8BNY6.

Proteomic databases

MaxQBiQ8BNY6.
PaxDbiQ8BNY6.
PeptideAtlasiQ8BNY6.
PRIDEiQ8BNY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000199; ENSMUSP00000000199; ENSMUSG00000062661.
GeneIDi14299.
KEGGimmu:14299.
UCSCiuc008jdq.1. mouse.

Organism-specific databases

CTDi23413.
MGIiMGI:109166. Ncs1.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ8BNY6.
KOiK19932.
OMAiFHEFIRA.
OrthoDBiEOG091G11T4.
PhylomeDBiQ8BNY6.
TreeFamiTF300009.

Miscellaneous databases

ChiTaRSiNcs1. mouse.
PROiQ8BNY6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000062661.
CleanExiMM_FREQ.
ExpressionAtlasiQ8BNY6. baseline and differential.
GenevisibleiQ8BNY6. MM.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCS1_MOUSE
AccessioniPrimary (citable) accession number: Q8BNY6
Secondary accession number(s): A2AJ84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 111 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds 3 calcium ions via the second, third and fourth EF-hand.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.