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Q8BNJ3

- NMNA2_MOUSE

UniProt

Q8BNJ3 - NMNA2_MOUSE

Protein

Nicotinamide mononucleotide adenylyltransferase 2

Gene

Nmnat2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity prefers NAD+, NADH and NAAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+ By similarity.By similarity

    Catalytic activityi

    ATP + nicotinamide ribonucleotide = diphosphate + NAD+.
    ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD+.

    Cofactori

    Divalent metal cations. Magnesium confers the highest activity By similarity.By similarity

    Enzyme regulationi

    Inhibited by P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate (Np3AD) and P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate (Np4AD).By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei16 – 161SubstrateBy similarity
    Binding sitei55 – 551SubstrateBy similarity
    Binding sitei202 – 2021SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi15 – 2410ATPSequence Analysis
    Nucleotide bindingi269 – 2746ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nicotinamide-nucleotide adenylyltransferase activity Source: UniProtKB-EC
    3. nicotinate-nucleotide adenylyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. NAD biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Pyridine nucleotide biosynthesis

    Keywords - Ligandi

    ATP-binding, NAD, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_209575. Nicotinate metabolism.
    UniPathwayiUPA00253; UER00600.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinamide mononucleotide adenylyltransferase 2 (EC:2.7.7.1)
    Short name:
    NMN adenylyltransferase 2
    Alternative name(s):
    Nicotinate-nucleotide adenylyltransferase 1 (EC:2.7.7.18)
    Short name:
    NaMN adenylyltransferase 1
    Gene namesi
    Name:Nmnat2
    Synonyms:Kiaa0479
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2444155. Nmnat2.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatus By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: MGI
    2. late endosome Source: MGI
    3. nucleus Source: Ensembl
    4. synapse Source: MGI
    5. trans-Golgi network Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 307307Nicotinamide mononucleotide adenylyltransferase 2PRO_0000135015Add
    BLAST

    Proteomic databases

    PRIDEiQ8BNJ3.

    PTM databases

    PhosphoSiteiQ8BNJ3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8BNJ3.
    BgeeiQ8BNJ3.
    CleanExiMM_NMNAT2.
    GenevestigatoriQ8BNJ3.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BNJ3.
    SMRiQ8BNJ3. Positions 9-109, 197-302.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1057.
    GeneTreeiENSGT00530000063189.
    HOGENOMiHOG000216047.
    HOVERGENiHBG052640.
    InParanoidiQ8BNJ3.
    KOiK06210.
    OMAiPMERFTF.
    OrthoDBiEOG7PVWPQ.
    PhylomeDBiQ8BNJ3.
    TreeFamiTF315035.

    Family and domain databases

    Gene3Di3.40.50.620. 2 hits.
    InterProiIPR004821. Cyt_trans-like.
    IPR005248. NAMN_adtrnsfrase.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR12039. PTHR12039. 1 hit.
    PfamiPF01467. CTP_transf_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BNJ3-1 [UniParc]FASTAAdd to Basket

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    MTETTKTHVI LLACGSFNPI TKGHIQMFER ARDYLHKTGR FIVIGGIVSP    50
    VHDSYGKQGL VSSRHRLIMC QLAVQNSDWI RVDPWECYQD TWQTTCSVLE 100
    HHRDLMKRVT GCILSNVNTP SMTPVIGQPQ HENTQPIYQN SNVPTKPTAA 150
    KILGKVGESL SRICCVRPPV ERFTFVDENA NLGTVMRYEE IELRILLLCG 200
    SDLLESFCIP GLWNEADMEV IVGDFGIVVV PRDAADTDRI MNHSSILRKY 250
    KNNIMVVKDD INHPMSVVSS TKSRLALQHG DGHVVDYLSQ PVIDYILKSQ 300
    LYINASG 307
    Length:307
    Mass (Da):34,505
    Last modified:March 1, 2003 - v1
    Checksum:iB9B4902D3EE03DC6
    GO

    Sequence cautioni

    The sequence BAC97965.1 differs from that shown. Reason: Frameshift at position 252.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK129155 mRNA. Translation: BAC97965.1. Frameshift.
    AK083532 mRNA. Translation: BAC38943.1.
    CCDSiCCDS15368.1.
    RefSeqiNP_780669.1. NM_175460.3.
    UniGeneiMm.40548.

    Genome annotation databases

    EnsembliENSMUST00000043313; ENSMUSP00000041110; ENSMUSG00000042751.
    GeneIDi226518.
    KEGGimmu:226518.
    UCSCiuc007czq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK129155 mRNA. Translation: BAC97965.1 . Frameshift.
    AK083532 mRNA. Translation: BAC38943.1 .
    CCDSi CCDS15368.1.
    RefSeqi NP_780669.1. NM_175460.3.
    UniGenei Mm.40548.

    3D structure databases

    ProteinModelPortali Q8BNJ3.
    SMRi Q8BNJ3. Positions 9-109, 197-302.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BNJ3.

    Proteomic databases

    PRIDEi Q8BNJ3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043313 ; ENSMUSP00000041110 ; ENSMUSG00000042751 .
    GeneIDi 226518.
    KEGGi mmu:226518.
    UCSCi uc007czq.1. mouse.

    Organism-specific databases

    CTDi 23057.
    MGIi MGI:2444155. Nmnat2.
    Rougei Search...

    Phylogenomic databases

    eggNOGi COG1057.
    GeneTreei ENSGT00530000063189.
    HOGENOMi HOG000216047.
    HOVERGENi HBG052640.
    InParanoidi Q8BNJ3.
    KOi K06210.
    OMAi PMERFTF.
    OrthoDBi EOG7PVWPQ.
    PhylomeDBi Q8BNJ3.
    TreeFami TF315035.

    Enzyme and pathway databases

    UniPathwayi UPA00253 ; UER00600 .
    Reactomei REACT_209575. Nicotinate metabolism.

    Miscellaneous databases

    NextBioi 378196.
    PROi Q8BNJ3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BNJ3.
    Bgeei Q8BNJ3.
    CleanExi MM_NMNAT2.
    Genevestigatori Q8BNJ3.

    Family and domain databases

    Gene3Di 3.40.50.620. 2 hits.
    InterProi IPR004821. Cyt_trans-like.
    IPR005248. NAMN_adtrnsfrase.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR12039. PTHR12039. 1 hit.
    Pfami PF01467. CTP_transf_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
      DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiNMNA2_MOUSE
    AccessioniPrimary (citable) accession number: Q8BNJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3