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Protein

Sulfhydryl oxidase 1

Gene

Qsox1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide. May contribute to disulfide bond formation in a variety of secreted proteins (By similarity).By similarity

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73NucleophileBy similarity1
Active sitei76NucleophileBy similarity1
Binding sitei404FADBy similarity1
Binding sitei411FADBy similarity1
Binding sitei415FADBy similarity1
Binding sitei454FADBy similarity1
Binding sitei458FADBy similarity1
Binding sitei503FADBy similarity1
Binding sitei506FADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi481 – 488FADBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.8.3.2. 3474.
ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfhydryl oxidase 1 (EC:1.8.3.2)
Short name:
mSOx
Alternative name(s):
Quiescin Q6
Skin sulfhydryl oxidase
Gene namesi
Name:Qsox1
Synonyms:Qscn6, Sox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1330818. Qsox1.

Subcellular locationi

Isoform 2 :
  • Secreted

  • Note: Found in the extracellular medium of quiescent cells but not in proliferating cells.
Isoform 3 :
  • Secreted

  • Note: Found in the extracellular medium of quiescent cells but not in proliferating cells.
Isoform 4 :
  • Secreted

  • Note: Found in the extracellular medium of quiescent cells but not in proliferating cells.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei711 – 731HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_000024953433 – 748Sulfhydryl oxidase 1Add BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi73 ↔ 76Redox-activePROSITE-ProRule annotation
Glycosylationi133N-linked (GlcNAc...)Sequence analysis1
Glycosylationi246N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi396 ↔ 408PROSITE-ProRule annotation
Disulfide bondi452 ↔ 455PROSITE-ProRule annotation
Disulfide bondi512 ↔ 515PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ8BND5.
PeptideAtlasiQ8BND5.
PRIDEiQ8BND5.

PTM databases

PhosphoSitePlusiQ8BND5.

Expressioni

Tissue specificityi

Expressed in the seminal vesicles and skin.1 Publication

Gene expression databases

BgeeiENSMUSG00000033684.
CleanExiMM_QSOX1.
GenevisibleiQ8BND5. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035658.

Structurei

Secondary structure

1748
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi41 – 44Combined sources4
Beta strandi46 – 48Combined sources3
Turni50 – 52Combined sources3
Helixi53 – 57Combined sources5
Beta strandi61 – 69Combined sources9
Helixi74 – 89Combined sources16
Helixi91 – 93Combined sources3
Turni94 – 96Combined sources3
Beta strandi97 – 103Combined sources7
Helixi107 – 115Combined sources9
Beta strandi120 – 127Combined sources8
Beta strandi133 – 135Combined sources3
Beta strandi137 – 139Combined sources3
Helixi146 – 159Combined sources14
Turni160 – 162Combined sources3
Helixi176 – 179Combined sources4
Helixi182 – 185Combined sources4
Beta strandi189 – 196Combined sources8
Helixi202 – 209Combined sources8
Turni210 – 212Combined sources3
Beta strandi216 – 222Combined sources7
Helixi226 – 231Combined sources6
Beta strandi236 – 244Combined sources9
Beta strandi249 – 251Combined sources3
Helixi259 – 267Combined sources9
Beta strandi269 – 271Combined sources3
Helixi293 – 296Combined sources4
Beta strandi301 – 303Combined sources3
Helixi304 – 316Combined sources13
Helixi319 – 321Combined sources3
Beta strandi323 – 326Combined sources4
Helixi327 – 343Combined sources17
Helixi348 – 363Combined sources16
Beta strandi367 – 370Combined sources4
Helixi371 – 380Combined sources10
Helixi383 – 386Combined sources4
Beta strandi403 – 405Combined sources3
Helixi406 – 426Combined sources21
Helixi437 – 449Combined sources13
Helixi453 – 466Combined sources14
Helixi467 – 469Combined sources3
Helixi473 – 491Combined sources19
Beta strandi505 – 507Combined sources3
Turni509 – 511Combined sources3
Helixi513 – 515Combined sources3
Helixi528 – 538Combined sources11
Helixi541 – 543Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T58X-ray2.40A/B/C/D36-550[»]
3T59X-ray2.80A/B/C/D36-550[»]
5D8IX-ray2.05A/B36-275[»]
5D93X-ray2.20A/D36-275[»]
5D96X-ray2.30A/D36-275[»]
ProteinModelPortaliQ8BND5.
SMRiQ8BND5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 159ThioredoxinPROSITE-ProRule annotationAdd BLAST127
Domaini399 – 506ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd BLAST108

Sequence similaritiesi

Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1731. Eukaryota.
ENOG410XVJT. LUCA.
GeneTreeiENSGT00390000008045.
HOVERGENiHBG080360.
InParanoidiQ8BND5.
KOiK10758.
OMAiKNFFGCT.
OrthoDBiEOG091G038J.
PhylomeDBiQ8BND5.
TreeFamiTF316749.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
SSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BND5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRRCGRLSGP PSLLLLLLLL SPLLFSGPGA YAARLSVLYS SSDPLTLLDA
60 70 80 90 100
DSVRPTVLGS SSAWAVEFFA SWCGHCIAFA PTWKELANDV KDWRPALNLA
110 120 130 140 150
VLDCAEETNS AVCREFNIAG FPTVRFFQAF TKNGSGATLP GAGANVQTLR
160 170 180 190 200
MRLIDALESH RDTWPPACPP LEPAKLNDID GFFTRNKADY LALVFEREDS
210 220 230 240 250
YLGREVTLDL SQYHAVAVRR VLNTESDLVN KFGVTDFPSC YLLLRNGSVS
260 270 280 290 300
RVPVLVESRS FYTSYLRGLP GLTRDAPPTT ATPVTADKIA PTVWKFADRS
310 320 330 340 350
KIYMADLESA LHYILRVEVG KFSVLEGQRL VALKKFVAVL AKYFPGQPLV
360 370 380 390 400
QNFLHSINDW LQKQQKKRIP YSFFKAALDS RKEDAVLTEK VNWVGCQGSE
410 420 430 440 450
PHFRGFPCSL WVLFHFLTVQ ANRYSEAHPQ EPADGQEVLQ AMRSYVQFFF
460 470 480 490 500
GCRDCADHFE QMAAASMHQV RSPSNAILWL WTSHNRVNAR LSGALSEDPH
510 520 530 540 550
FPKVQWPPRE LCSACHNELN GQVPLWDLGA TLNFLKAHFS PANIVIDSSA
560 570 580 590 600
SRHTGRRGSP EATPELVMDT LKLESRNSVL GHEQAASAES PGATALDVPA
610 620 630 640 650
EKPEASGPQE LYTGLRMGGA SPGQGPPERM EDHQRDMQEN APGQQHLSKR
660 670 680 690 700
DTEALFLPEV NHLQGPLELR RGGRSPKQLA PILEEEPEAL AIQGQGQWLQ
710 720 730 740
VLGGGISHLD ISLCVGLYSV SFMGLLAMYT YFRARLRTPK GHASYPTA
Length:748
Mass (Da):82,785
Last modified:March 1, 2003 - v1
Checksum:i0499F6B8DAB5A8D1
GO
Isoform 2 (identifier: Q8BND5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     660-661: VN → LL
     662-748: Missing.

Note: No experimental confirmation available.
Show »
Length:661
Mass (Da):73,297
Checksum:iAFC4B43576BEE56B
GO
Isoform 3 (identifier: Q8BND5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     567-568: VM → LL
     569-748: Missing.

Show »
Length:568
Mass (Da):63,336
Checksum:i761F44799536DBBB
GO
Isoform 4 (identifier: Q8BND5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     126-154: FFQAFTKNGSGATLPGAGANVQTLRMRLI → VCEGGATVEWFWPSTQSSVPHFPGTFLGC
     155-748: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:154
Mass (Da):16,624
Checksum:iF13FDA0BC3335587
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti637M → I in BAE41429 (PubMed:16141072).Curated1
Sequence conflicti656F → L in BAE41429 (PubMed:16141072).Curated1
Isoform 4 (identifier: Q8BND5-4)
Sequence conflicti127C → Y in BAE37202 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020491126 – 154FFQAF…RMRLI → VCEGGATVEWFWPSTQSSVP HFPGTFLGC in isoform 4. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_020492155 – 748Missing in isoform 4. 1 PublicationAdd BLAST594
Alternative sequenceiVSP_020493567 – 568VM → LL in isoform 3. 3 Publications2
Alternative sequenceiVSP_020494569 – 748Missing in isoform 3. 3 PublicationsAdd BLAST180
Alternative sequenceiVSP_020495660 – 661VN → LL in isoform 2. 1 Publication2
Alternative sequenceiVSP_020496662 – 748Missing in isoform 2. 1 PublicationAdd BLAST87

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044284 mRNA. Translation: BAB21936.1.
AK004880 mRNA. Translation: BAB23638.1.
AK083938 mRNA. Translation: BAC39073.1.
AK149465 mRNA. Translation: BAE28897.1.
AK163119 mRNA. Translation: BAE37202.1.
AK166839 mRNA. Translation: BAE39061.1.
AK169877 mRNA. Translation: BAE41429.1.
AK169920 mRNA. Translation: BAE41459.1.
AK170449 mRNA. Translation: BAE41807.1.
BC034131 mRNA. Translation: AAH34131.1.
BC076590 mRNA. Translation: AAH76590.1.
CCDSiCCDS15386.1. [Q8BND5-1]
CCDS78713.1. [Q8BND5-3]
RefSeqiNP_001020116.1. NM_001024945.1. [Q8BND5-1]
NP_075757.1. NM_023268.2. [Q8BND5-3]
UniGeneiMm.27035.

Genome annotation databases

EnsembliENSMUST00000035325; ENSMUSP00000035658; ENSMUSG00000033684. [Q8BND5-1]
ENSMUST00000111764; ENSMUSP00000107394; ENSMUSG00000033684. [Q8BND5-2]
ENSMUST00000194632; ENSMUSP00000142301; ENSMUSG00000033684. [Q8BND5-3]
GeneIDi104009.
KEGGimmu:104009.
UCSCiuc007dbo.1. mouse. [Q8BND5-3]
uc007dbp.1. mouse. [Q8BND5-1]
uc007dbq.1. mouse. [Q8BND5-4]
uc011wtx.1. mouse. [Q8BND5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044284 mRNA. Translation: BAB21936.1.
AK004880 mRNA. Translation: BAB23638.1.
AK083938 mRNA. Translation: BAC39073.1.
AK149465 mRNA. Translation: BAE28897.1.
AK163119 mRNA. Translation: BAE37202.1.
AK166839 mRNA. Translation: BAE39061.1.
AK169877 mRNA. Translation: BAE41429.1.
AK169920 mRNA. Translation: BAE41459.1.
AK170449 mRNA. Translation: BAE41807.1.
BC034131 mRNA. Translation: AAH34131.1.
BC076590 mRNA. Translation: AAH76590.1.
CCDSiCCDS15386.1. [Q8BND5-1]
CCDS78713.1. [Q8BND5-3]
RefSeqiNP_001020116.1. NM_001024945.1. [Q8BND5-1]
NP_075757.1. NM_023268.2. [Q8BND5-3]
UniGeneiMm.27035.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3T58X-ray2.40A/B/C/D36-550[»]
3T59X-ray2.80A/B/C/D36-550[»]
5D8IX-ray2.05A/B36-275[»]
5D93X-ray2.20A/D36-275[»]
5D96X-ray2.30A/D36-275[»]
ProteinModelPortaliQ8BND5.
SMRiQ8BND5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000035658.

PTM databases

PhosphoSitePlusiQ8BND5.

Proteomic databases

PaxDbiQ8BND5.
PeptideAtlasiQ8BND5.
PRIDEiQ8BND5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035325; ENSMUSP00000035658; ENSMUSG00000033684. [Q8BND5-1]
ENSMUST00000111764; ENSMUSP00000107394; ENSMUSG00000033684. [Q8BND5-2]
ENSMUST00000194632; ENSMUSP00000142301; ENSMUSG00000033684. [Q8BND5-3]
GeneIDi104009.
KEGGimmu:104009.
UCSCiuc007dbo.1. mouse. [Q8BND5-3]
uc007dbp.1. mouse. [Q8BND5-1]
uc007dbq.1. mouse. [Q8BND5-4]
uc011wtx.1. mouse. [Q8BND5-2]

Organism-specific databases

CTDi5768.
MGIiMGI:1330818. Qsox1.

Phylogenomic databases

eggNOGiKOG1731. Eukaryota.
ENOG410XVJT. LUCA.
GeneTreeiENSGT00390000008045.
HOVERGENiHBG080360.
InParanoidiQ8BND5.
KOiK10758.
OMAiKNFFGCT.
OrthoDBiEOG091G038J.
PhylomeDBiQ8BND5.
TreeFamiTF316749.

Enzyme and pathway databases

BRENDAi1.8.3.2. 3474.
ReactomeiR-MMU-114608. Platelet degranulation.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiQ8BND5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033684.
CleanExiMM_QSOX1.
GenevisibleiQ8BND5. MM.

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF04777. Evr1_Alr. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
SSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQSOX1_MOUSE
AccessioniPrimary (citable) accession number: Q8BND5
Secondary accession number(s): Q3TDY9
, Q3TE19, Q3TR29, Q3UEL4, Q8K041, Q9DBL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.