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Protein

WD repeat-containing protein 35

Gene

Wdr35

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May promote CASP3 activation and TNF-stimulated apoptosis (By similarity). Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport. Required for ciliogenesis.By similarity1 Publication

GO - Biological processi

  • cilium assembly Source: UniProtKB
  • intraciliary retrograde transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Enzyme and pathway databases

ReactomeiR-MMU-5610787. Hedgehog 'off' state.
R-MMU-5620924. Intraflagellar transport.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein 35
Gene namesi
Name:Wdr35
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1921932. Wdr35.

Subcellular locationi

GO - Cellular componenti

  • axoneme Source: UniProtKB
  • centrosome Source: UniProtKB
  • ciliary basal body Source: UniProtKB
  • cytoplasm Source: UniProtKB-KW
  • intraciliary transport particle A Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11811181WD repeat-containing protein 35PRO_0000051385Add
BLAST

Proteomic databases

MaxQBiQ8BND3.
PaxDbiQ8BND3.
PRIDEiQ8BND3.

PTM databases

iPTMnetiQ8BND3.
PhosphoSiteiQ8BND3.

Expressioni

Gene expression databases

BgeeiQ8BND3.
CleanExiMM_WDR35.
ExpressionAtlasiQ8BND3. baseline and differential.
GenevisibleiQ8BND3. MM.

Interactioni

Subunit structurei

Component of the IFT complex A (IFT-A). Interacts with IFT43 (By similarity).By similarity

Protein-protein interaction databases

BioGridi216937. 1 interaction.
STRINGi10090.ENSMUSP00000082895.

Structurei

3D structure databases

ProteinModelPortaliQ8BND3.
SMRiQ8BND3. Positions 20-186, 285-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati12 – 5140WD 1Add
BLAST
Repeati69 – 10840WD 2Add
BLAST
Repeati113 – 15240WD 3Add
BLAST
Repeati154 – 19340WD 4Add
BLAST
Repeati502 – 53938WD 5Add
BLAST

Sequence similaritiesi

Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2041. Eukaryota.
ENOG410XNSQ. LUCA.
GeneTreeiENSGT00610000085970.
HOGENOMiHOG000255527.
HOVERGENiHBG079343.
InParanoidiQ8BND3.
KOiK19674.
OMAiLINFEIR.
OrthoDBiEOG7QRQT6.
TreeFamiTF314076.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR024977. Apc4_WD40_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR017233. WD_repeat_p35.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
[Graphical view]
PIRSFiPIRSF037536. WD_repeat_p35. 1 hit.
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BND3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFFYLSKKIA VPNNVKLKCI SWNKDQGFIA CGGEDGLLKV LRLETQTDDS
60 70 80 90 100
KLRGLAAPSN LSMNQNLEGH SGAVQVVTWN EQYQKLTTSD QNGLIIVWML
110 120 130 140 150
YKGSWYEEMI NNRNKSVVRS MSWNADGQKI CIVYEDGAVI VGSVDGNRIW
160 170 180 190 200
GKDLKGIQLC HVTWSADSKI LLFGMANGEI HIYDNQGNFI MKMKLNCLVN
210 220 230 240 250
VTGAISIAGI HWYHGTEGYV EPDCPCLAIC FDNGRCQIMR HENDQNPVLI
260 270 280 290 300
DTGMYVVGIQ WNHIGSVLAV AGSQKVVTQD KDINIVQFYT PFGEHLGTLK
310 320 330 340 350
VPGKQMCSLS WEGGGLKIAL AVDSFIYFAN IRPDYKWGYC SNTVVYAYTR
360 370 380 390 400
PDRPEYCVVF WDTKNSEKYV KYVKSLISIT TCGDFCILAT KADENHPQEE
410 420 430 440 450
NEMETFGATF VLVLCNSIGT PLDPKYIDLV PLFVAMTKTH VIAASKEAFY
460 470 480 490 500
TWQYRVAKKL TALEINQITR SRKEGRERIY HVDDVPSGSV DGVFDYSKAI
510 520 530 540 550
QGTRDPICAI TASDKTLIVG RESGVIQRYS FPNVALIQKY SLDCRACQLS
560 570 580 590 600
LNCNSSRLAI IDIAGVLTFF DLDTRVTDST GQQVVGELLK LERKDVWDMK
610 620 630 640 650
WAKDNPDLFA MMEKTRMYVF RNLDPEEPIQ TSGYICNFED LEIKSVLLDE
660 670 680 690 700
ILKDPEHPSK DYIMNFEIRS LRDSRALIEK VGIEDASQFI EDNPHPRLWR
710 720 730 740 750
LLAEAALQKL DLYTAQQAFV RCKDYQGIKF VKLLGNLQSE SMKQAEVIAY
760 770 780 790 800
FGRFEDAERM YQDMDRRDLA IGLRMKLGDW FRVLQLLKTG SGDADDSLLE
810 820 830 840 850
QANNAIGEYF ADRQKWQNAV QYYVKGRNQE RLAECYYMLE DYEGLETLAN
860 870 880 890 900
SLPENHKLLP EIAQMFVRVG MCEQAVSAFL KCNQPKAAVD TCVHLNQWNK
910 920 930 940 950
AVELAKSHSM KEIGSLLARY ASHLLEKNKT LDAIELYRKA SYFFDAAKLM
960 970 980 990 1000
YKIADEEAKK RTKPLRVKKL YVLSALLIEQ YHEQMKNAQR GKVKGKNSEA
1010 1020 1030 1040 1050
TSALAGLLEE EVLSTTSRFT DNAWRGAEAY HFFILAQRQL YEGYVDTALK
1060 1070 1080 1090 1100
TALHLRDYED IIPSVEIYSL LALCACASRA FGTCSKAFIK LESLETLSAE
1110 1120 1130 1140 1150
QKQQYEDLAL EIFTKHTPKD NRKSELNSLL EGGEGKLPTC IATGSPIIEY
1160 1170 1180
QFWVCKVCKH YVLAQEISNY NFCPLCHSSV E
Length:1,181
Mass (Da):133,991
Last modified:July 27, 2011 - v3
Checksum:iF69BA836E9CA65CE
GO
Isoform 2 (identifier: Q8BND3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     399-409: Missing.

Show »
Length:1,170
Mass (Da):132,752
Checksum:iC74FFD8594961B2E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti634 – 6341Y → H in AAH56925 (PubMed:15489334).Curated
Sequence conflicti828 – 8281N → D in BAC39081 (PubMed:16141072).Curated
Sequence conflicti915 – 9151S → Y in BAC26663 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei399 – 40911Missing in isoform 2. 1 PublicationVSP_009733Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029896 mRNA. Translation: BAC26663.1.
AK083971 mRNA. Translation: BAC39081.1.
AC110376 Genomic DNA. No translation available.
BC056925 mRNA. Translation: AAH56925.1.
CCDSiCCDS25807.1. [Q8BND3-1]
CCDS49027.1. [Q8BND3-2]
RefSeqiNP_001152999.1. NM_001159527.1. [Q8BND3-2]
NP_766058.3. NM_172470.3. [Q8BND3-1]
UniGeneiMm.87389.

Genome annotation databases

EnsembliENSMUST00000085745; ENSMUSP00000082895; ENSMUSG00000066643. [Q8BND3-1]
ENSMUST00000111113; ENSMUSP00000106742; ENSMUSG00000066643. [Q8BND3-2]
GeneIDi74682.
KEGGimmu:74682.
UCSCiuc007naj.2. mouse. [Q8BND3-1]
uc007nak.2. mouse. [Q8BND3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029896 mRNA. Translation: BAC26663.1.
AK083971 mRNA. Translation: BAC39081.1.
AC110376 Genomic DNA. No translation available.
BC056925 mRNA. Translation: AAH56925.1.
CCDSiCCDS25807.1. [Q8BND3-1]
CCDS49027.1. [Q8BND3-2]
RefSeqiNP_001152999.1. NM_001159527.1. [Q8BND3-2]
NP_766058.3. NM_172470.3. [Q8BND3-1]
UniGeneiMm.87389.

3D structure databases

ProteinModelPortaliQ8BND3.
SMRiQ8BND3. Positions 20-186, 285-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi216937. 1 interaction.
STRINGi10090.ENSMUSP00000082895.

PTM databases

iPTMnetiQ8BND3.
PhosphoSiteiQ8BND3.

Proteomic databases

MaxQBiQ8BND3.
PaxDbiQ8BND3.
PRIDEiQ8BND3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085745; ENSMUSP00000082895; ENSMUSG00000066643. [Q8BND3-1]
ENSMUST00000111113; ENSMUSP00000106742; ENSMUSG00000066643. [Q8BND3-2]
GeneIDi74682.
KEGGimmu:74682.
UCSCiuc007naj.2. mouse. [Q8BND3-1]
uc007nak.2. mouse. [Q8BND3-2]

Organism-specific databases

CTDi57539.
MGIiMGI:1921932. Wdr35.

Phylogenomic databases

eggNOGiKOG2041. Eukaryota.
ENOG410XNSQ. LUCA.
GeneTreeiENSGT00610000085970.
HOGENOMiHOG000255527.
HOVERGENiHBG079343.
InParanoidiQ8BND3.
KOiK19674.
OMAiLINFEIR.
OrthoDBiEOG7QRQT6.
TreeFamiTF314076.

Enzyme and pathway databases

ReactomeiR-MMU-5610787. Hedgehog 'off' state.
R-MMU-5620924. Intraflagellar transport.

Miscellaneous databases

ChiTaRSiWdr35. mouse.
NextBioi341374.
PROiQ8BND3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8BND3.
CleanExiMM_WDR35.
ExpressionAtlasiQ8BND3. baseline and differential.
GenevisibleiQ8BND3. MM.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR024977. Apc4_WD40_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR017233. WD_repeat_p35.
[Graphical view]
PfamiPF12894. ANAPC4_WD40. 1 hit.
[Graphical view]
PIRSFiPIRSF037536. WD_repeat_p35. 1 hit.
SMARTiSM00320. WD40. 4 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Spinal ganglion and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  5. Cited for: FUNCTION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiWDR35_MOUSE
AccessioniPrimary (citable) accession number: Q8BND3
Secondary accession number(s): E9QLW3, Q8CDL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.