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Q8BN82

- S17A5_MOUSE

UniProt

Q8BN82 - S17A5_MOUSE

Protein

Sialin

Gene

Slc17a5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 2 (07 Jun 2004)
      Previous versions | rss
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    Functioni

    Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential-dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO3-/H+ cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva By similarity.By similarity

    GO - Molecular functioni

    1. sialic acid transmembrane transporter activity Source: Ensembl
    2. symporter activity Source: UniProtKB-KW

    GO - Biological processi

    1. amino acid transport Source: UniProtKB-KW
    2. sialic acid transport Source: Ensembl

    Keywords - Biological processi

    Amino-acid transport, Symport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_202500. Sialic acid metabolism.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialin
    Alternative name(s):
    H(+)/nitrate cotransporter
    H(+)/sialic acid cotransporter
    Short name:
    AST
    Solute carrier family 17 member 5
    Vesicular H(+)/Aspartate-glutamate cotransporter
    Gene namesi
    Name:Slc17a5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1924105. Slc17a5.

    Subcellular locationi

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytoplasmic membrane-bounded vesicle Source: MGI
    3. integral component of membrane Source: UniProtKB-KW
    4. lysosomal membrane Source: UniProtKB-SubCell
    5. plasma membrane Source: MGI
    6. synaptic vesicle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 495495SialinPRO_0000220948Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8BN82.

    PTM databases

    PhosphoSiteiQ8BN82.

    Expressioni

    Gene expression databases

    BgeeiQ8BN82.
    GenevestigatoriQ8BN82.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BN82.
    SMRiQ8BN82. Positions 114-142.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4141CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini63 – 10947LumenalSequence AnalysisAdd
    BLAST
    Topological domaini131 – 1366CytoplasmicSequence Analysis
    Topological domaini158 – 1581LumenalSequence Analysis
    Topological domaini180 – 20021CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini222 – 2276LumenalSequence Analysis
    Topological domaini249 – 27830CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini300 – 32829LumenalSequence AnalysisAdd
    BLAST
    Topological domaini350 – 36516CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini387 – 3915LumenalSequence Analysis
    Topological domaini413 – 42311CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini445 – 45713LumenalSequence AnalysisAdd
    BLAST
    Topological domaini479 – 49517CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei42 – 6221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei110 – 13021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei137 – 15721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei159 – 17921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei201 – 22121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei228 – 24821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei279 – 29921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei329 – 34921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei366 – 38621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei392 – 41221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei424 – 44421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei458 – 47821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi22 – 232Dileucine internalization motifBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00640000091442.
    HOGENOMiHOG000230811.
    HOVERGENiHBG008834.
    InParanoidiQ8BN82.
    KOiK12301.
    OMAiKHKRISH.
    OrthoDBiEOG789C9Z.
    PhylomeDBiQ8BN82.
    TreeFamiTF313535.

    Family and domain databases

    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BN82-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRPLLRGPAG NDDEESSDST PLLPGARQTE AAPVCCSARY NLAILAFCGF    50
    FVLYALRVNL SVALVDMVDS NTTLTDNRTS KECAEHSAPI KVHHNHTGKK 100
    YKWDAETQGW ILGSFFYGYI VTQIPGGYIA SRVGGKLLLG LGILGTSVFT 150
    LFTPLAADLG VVTLVVLRAL EGLGEGVTFP AMHAMWSSWA PPLERSKLLT 200
    ISYAGAQLGT VISLPLSGII CYYMNWTYVF YLFGIVGIVW FILWMWIVSD 250
    TPETHKTISH YEKEYIVSSL KNQLSSQKVV PWGSILKSLP LWAIVVAHFS 300
    YNWSFYTLLT LLPTYMKEIL RFNVQENGFL SALPYFGCWL CMILCGQAAD 350
    YLRVKWNFST ISVRRIFSLV GMVGPAVFLV AAGFIGCDYS LAVAFLTIST 400
    TLGGFASSGF SINHLDIAPS YAGILLGITN TFATIPGMTG PIIAKSLTPD 450
    NTIREWQTVF CIAAAINVFG AIFFTLFAKG EVQSWALSDH HGHRN 495
    Length:495
    Mass (Da):54,369
    Last modified:June 7, 2004 - v2
    Checksum:i6009661215D26437
    GO
    Isoform 2 (identifier: Q8BN82-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         98-123: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:469
    Mass (Da):51,301
    Checksum:iB48BCF204A2D6008
    GO
    Isoform 3 (identifier: Q8BN82-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         176-238: GVTFPAMHAM...VFYLFGIVGI → KYPPPGCYVS...YELDLRLLSF
         239-495: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:238
    Mass (Da):25,879
    Checksum:i95222793B0E39762
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei98 – 12326Missing in isoform 2. 1 PublicationVSP_010484Add
    BLAST
    Alternative sequencei176 – 23863GVTFP…GIVGI → KYPPPGCYVSSYARHVVFLG SPSGKKQASYHFLCGSTAWD SDLTSSFRNNMLLYELDLRL LSF in isoform 3. 1 PublicationVSP_010485Add
    BLAST
    Alternative sequencei239 – 495257Missing in isoform 3. 1 PublicationVSP_010486Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK029102 mRNA. Translation: BAC26298.1.
    AK087395 mRNA. Translation: BAC39859.1.
    AK169868 mRNA. Translation: BAE41423.1.
    BC058785 mRNA. Translation: AAH58785.1.
    CCDSiCCDS23364.1. [Q8BN82-1]
    CCDS72283.1. [Q8BN82-2]
    RefSeqiNP_001263381.1. NM_001276452.1. [Q8BN82-2]
    NP_766361.1. NM_172773.3. [Q8BN82-1]
    UniGeneiMm.46932.

    Genome annotation databases

    EnsembliENSMUST00000052441; ENSMUSP00000056182; ENSMUSG00000049624. [Q8BN82-1]
    ENSMUST00000117645; ENSMUSP00000113003; ENSMUSG00000049624. [Q8BN82-2]
    ENSMUST00000119213; ENSMUSP00000113340; ENSMUSG00000049624. [Q8BN82-3]
    GeneIDi235504.
    KEGGimmu:235504.
    UCSCiuc009quo.1. mouse. [Q8BN82-1]
    uc009qup.1. mouse. [Q8BN82-3]
    uc012gxg.1. mouse. [Q8BN82-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK029102 mRNA. Translation: BAC26298.1 .
    AK087395 mRNA. Translation: BAC39859.1 .
    AK169868 mRNA. Translation: BAE41423.1 .
    BC058785 mRNA. Translation: AAH58785.1 .
    CCDSi CCDS23364.1. [Q8BN82-1 ]
    CCDS72283.1. [Q8BN82-2 ]
    RefSeqi NP_001263381.1. NM_001276452.1. [Q8BN82-2 ]
    NP_766361.1. NM_172773.3. [Q8BN82-1 ]
    UniGenei Mm.46932.

    3D structure databases

    ProteinModelPortali Q8BN82.
    SMRi Q8BN82. Positions 114-142.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8BN82.

    Proteomic databases

    PRIDEi Q8BN82.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000052441 ; ENSMUSP00000056182 ; ENSMUSG00000049624 . [Q8BN82-1 ]
    ENSMUST00000117645 ; ENSMUSP00000113003 ; ENSMUSG00000049624 . [Q8BN82-2 ]
    ENSMUST00000119213 ; ENSMUSP00000113340 ; ENSMUSG00000049624 . [Q8BN82-3 ]
    GeneIDi 235504.
    KEGGi mmu:235504.
    UCSCi uc009quo.1. mouse. [Q8BN82-1 ]
    uc009qup.1. mouse. [Q8BN82-3 ]
    uc012gxg.1. mouse. [Q8BN82-2 ]

    Organism-specific databases

    CTDi 26503.
    MGIi MGI:1924105. Slc17a5.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00640000091442.
    HOGENOMi HOG000230811.
    HOVERGENi HBG008834.
    InParanoidi Q8BN82.
    KOi K12301.
    OMAi KHKRISH.
    OrthoDBi EOG789C9Z.
    PhylomeDBi Q8BN82.
    TreeFami TF313535.

    Enzyme and pathway databases

    Reactomei REACT_202500. Sialic acid metabolism.

    Miscellaneous databases

    ChiTaRSi SLC17A5. mouse.
    NextBioi 382720.
    PROi Q8BN82.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BN82.
    Genevestigatori Q8BN82.

    Family and domain databases

    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 2 hits.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
      Strain: C57BL/6J and NOD.
      Tissue: Eye and Skin.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Olfactory epithelium.
    3. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    4. "The lysosomal sialic acid transporter sialin is required for normal CNS myelination."
      Prolo L.M., Vogel H., Reimer R.J.
      J. Neurosci. 29:15355-15365(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiS17A5_MOUSE
    AccessioniPrimary (citable) accession number: Q8BN82
    Secondary accession number(s): Q3TE25
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 7, 2004
    Last modified: October 1, 2014
    This is version 89 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3