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Q8BN82

- S17A5_MOUSE

UniProt

Q8BN82 - S17A5_MOUSE

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Protein
Sialin
Gene
Slc17a5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential-dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO3-/H+ cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva By similarity.1 Publication

GO - Molecular functioni

  1. sialic acid transmembrane transporter activity Source: Ensembl
  2. symporter activity Source: UniProtKB-KW

GO - Biological processi

  1. amino acid transport Source: UniProtKB-KW
  2. sialic acid transport Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Symport, Transport

Enzyme and pathway databases

ReactomeiREACT_202500. Sialic acid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialin
Alternative name(s):
H(+)/nitrate cotransporter
H(+)/sialic acid cotransporter
Short name:
AST
Solute carrier family 17 member 5
Vesicular H(+)/Aspartate-glutamate cotransporter
Gene namesi
Name:Slc17a5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1924105. Slc17a5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4141Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei42 – 6221Helical; Reviewed prediction
Add
BLAST
Topological domaini63 – 10947Lumenal Reviewed prediction
Add
BLAST
Transmembranei110 – 13021Helical; Reviewed prediction
Add
BLAST
Topological domaini131 – 1366Cytoplasmic Reviewed prediction
Transmembranei137 – 15721Helical; Reviewed prediction
Add
BLAST
Topological domaini158 – 1581Lumenal Reviewed prediction
Transmembranei159 – 17921Helical; Reviewed prediction
Add
BLAST
Topological domaini180 – 20021Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei201 – 22121Helical; Reviewed prediction
Add
BLAST
Topological domaini222 – 2276Lumenal Reviewed prediction
Transmembranei228 – 24821Helical; Reviewed prediction
Add
BLAST
Topological domaini249 – 27830Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei279 – 29921Helical; Reviewed prediction
Add
BLAST
Topological domaini300 – 32829Lumenal Reviewed prediction
Add
BLAST
Transmembranei329 – 34921Helical; Reviewed prediction
Add
BLAST
Topological domaini350 – 36516Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei366 – 38621Helical; Reviewed prediction
Add
BLAST
Topological domaini387 – 3915Lumenal Reviewed prediction
Transmembranei392 – 41221Helical; Reviewed prediction
Add
BLAST
Topological domaini413 – 42311Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei424 – 44421Helical; Reviewed prediction
Add
BLAST
Topological domaini445 – 45713Lumenal Reviewed prediction
Add
BLAST
Transmembranei458 – 47821Helical; Reviewed prediction
Add
BLAST
Topological domaini479 – 49517Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cytoplasmic membrane-bounded vesicle Source: MGI
  3. integral component of membrane Source: UniProtKB-KW
  4. lysosomal membrane Source: UniProtKB-SubCell
  5. plasma membrane Source: MGI
  6. synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Sialin
PRO_0000220948Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi77 – 771N-linked (GlcNAc...) Reviewed prediction
Glycosylationi95 – 951N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8BN82.

PTM databases

PhosphoSiteiQ8BN82.

Expressioni

Gene expression databases

BgeeiQ8BN82.
GenevestigatoriQ8BN82.

Structurei

3D structure databases

ProteinModelPortaliQ8BN82.
SMRiQ8BN82. Positions 114-142.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi22 – 232Dileucine internalization motif By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00640000091442.
HOGENOMiHOG000230811.
HOVERGENiHBG008834.
InParanoidiQ8BN82.
KOiK12301.
OMAiKHKRISH.
OrthoDBiEOG789C9Z.
PhylomeDBiQ8BN82.
TreeFamiTF313535.

Family and domain databases

InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BN82-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MRPLLRGPAG NDDEESSDST PLLPGARQTE AAPVCCSARY NLAILAFCGF    50
FVLYALRVNL SVALVDMVDS NTTLTDNRTS KECAEHSAPI KVHHNHTGKK 100
YKWDAETQGW ILGSFFYGYI VTQIPGGYIA SRVGGKLLLG LGILGTSVFT 150
LFTPLAADLG VVTLVVLRAL EGLGEGVTFP AMHAMWSSWA PPLERSKLLT 200
ISYAGAQLGT VISLPLSGII CYYMNWTYVF YLFGIVGIVW FILWMWIVSD 250
TPETHKTISH YEKEYIVSSL KNQLSSQKVV PWGSILKSLP LWAIVVAHFS 300
YNWSFYTLLT LLPTYMKEIL RFNVQENGFL SALPYFGCWL CMILCGQAAD 350
YLRVKWNFST ISVRRIFSLV GMVGPAVFLV AAGFIGCDYS LAVAFLTIST 400
TLGGFASSGF SINHLDIAPS YAGILLGITN TFATIPGMTG PIIAKSLTPD 450
NTIREWQTVF CIAAAINVFG AIFFTLFAKG EVQSWALSDH HGHRN 495
Length:495
Mass (Da):54,369
Last modified:June 7, 2004 - v2
Checksum:i6009661215D26437
GO
Isoform 2 (identifier: Q8BN82-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-123: Missing.

Note: No experimental confirmation available.

Show »
Length:469
Mass (Da):51,301
Checksum:iB48BCF204A2D6008
GO
Isoform 3 (identifier: Q8BN82-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     176-238: GVTFPAMHAM...VFYLFGIVGI → KYPPPGCYVS...YELDLRLLSF
     239-495: Missing.

Note: No experimental confirmation available.

Show »
Length:238
Mass (Da):25,879
Checksum:i95222793B0E39762
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei98 – 12326Missing in isoform 2.
VSP_010484Add
BLAST
Alternative sequencei176 – 23863GVTFP…GIVGI → KYPPPGCYVSSYARHVVFLG SPSGKKQASYHFLCGSTAWD SDLTSSFRNNMLLYELDLRL LSF in isoform 3.
VSP_010485Add
BLAST
Alternative sequencei239 – 495257Missing in isoform 3.
VSP_010486Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK029102 mRNA. Translation: BAC26298.1.
AK087395 mRNA. Translation: BAC39859.1.
AK169868 mRNA. Translation: BAE41423.1.
BC058785 mRNA. Translation: AAH58785.1.
CCDSiCCDS23364.1. [Q8BN82-1]
CCDS72283.1. [Q8BN82-2]
RefSeqiNP_001263381.1. NM_001276452.1. [Q8BN82-2]
NP_766361.1. NM_172773.3. [Q8BN82-1]
UniGeneiMm.46932.

Genome annotation databases

EnsembliENSMUST00000052441; ENSMUSP00000056182; ENSMUSG00000049624. [Q8BN82-1]
ENSMUST00000117645; ENSMUSP00000113003; ENSMUSG00000049624. [Q8BN82-2]
ENSMUST00000119213; ENSMUSP00000113340; ENSMUSG00000049624. [Q8BN82-3]
GeneIDi235504.
KEGGimmu:235504.
UCSCiuc009quo.1. mouse. [Q8BN82-1]
uc009qup.1. mouse. [Q8BN82-3]
uc012gxg.1. mouse. [Q8BN82-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK029102 mRNA. Translation: BAC26298.1 .
AK087395 mRNA. Translation: BAC39859.1 .
AK169868 mRNA. Translation: BAE41423.1 .
BC058785 mRNA. Translation: AAH58785.1 .
CCDSi CCDS23364.1. [Q8BN82-1 ]
CCDS72283.1. [Q8BN82-2 ]
RefSeqi NP_001263381.1. NM_001276452.1. [Q8BN82-2 ]
NP_766361.1. NM_172773.3. [Q8BN82-1 ]
UniGenei Mm.46932.

3D structure databases

ProteinModelPortali Q8BN82.
SMRi Q8BN82. Positions 114-142.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q8BN82.

Proteomic databases

PRIDEi Q8BN82.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000052441 ; ENSMUSP00000056182 ; ENSMUSG00000049624 . [Q8BN82-1 ]
ENSMUST00000117645 ; ENSMUSP00000113003 ; ENSMUSG00000049624 . [Q8BN82-2 ]
ENSMUST00000119213 ; ENSMUSP00000113340 ; ENSMUSG00000049624 . [Q8BN82-3 ]
GeneIDi 235504.
KEGGi mmu:235504.
UCSCi uc009quo.1. mouse. [Q8BN82-1 ]
uc009qup.1. mouse. [Q8BN82-3 ]
uc012gxg.1. mouse. [Q8BN82-2 ]

Organism-specific databases

CTDi 26503.
MGIi MGI:1924105. Slc17a5.

Phylogenomic databases

eggNOGi COG0477.
GeneTreei ENSGT00640000091442.
HOGENOMi HOG000230811.
HOVERGENi HBG008834.
InParanoidi Q8BN82.
KOi K12301.
OMAi KHKRISH.
OrthoDBi EOG789C9Z.
PhylomeDBi Q8BN82.
TreeFami TF313535.

Enzyme and pathway databases

Reactomei REACT_202500. Sialic acid metabolism.

Miscellaneous databases

ChiTaRSi SLC17A5. mouse.
NextBioi 382720.
PROi Q8BN82.
SOURCEi Search...

Gene expression databases

Bgeei Q8BN82.
Genevestigatori Q8BN82.

Family and domain databases

InterProi IPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
Pfami PF07690. MFS_1. 1 hit.
[Graphical view ]
SUPFAMi SSF103473. SSF103473. 2 hits.
PROSITEi PS50850. MFS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Eye and Skin.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Olfactory epithelium.
  3. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  4. "The lysosomal sialic acid transporter sialin is required for normal CNS myelination."
    Prolo L.M., Vogel H., Reimer R.J.
    J. Neurosci. 29:15355-15365(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiS17A5_MOUSE
AccessioniPrimary (citable) accession number: Q8BN82
Secondary accession number(s): Q3TE25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 3, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi