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Protein

Sialin

Gene

Slc17a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential-dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO3-/H+ cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Symport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.
R-MMU-428643. Organic anion transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialin
Alternative name(s):
H(+)/nitrate cotransporter
H(+)/sialic acid cotransporter
Short name:
AST
Solute carrier family 17 member 5
Vesicular H(+)/Aspartate-glutamate cotransporter
Gene namesi
Name:Slc17a5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1924105. Slc17a5.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Cytoplasmic vesiclesecretory vesiclesynaptic vesicle membrane By similarity; Multi-pass membrane protein By similarity
  • Lysosome membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 41CytoplasmicSequence analysisAdd BLAST41
Transmembranei42 – 62HelicalSequence analysisAdd BLAST21
Topological domaini63 – 109LumenalSequence analysisAdd BLAST47
Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
Topological domaini131 – 136CytoplasmicSequence analysis6
Transmembranei137 – 157HelicalSequence analysisAdd BLAST21
Topological domaini158LumenalSequence analysis1
Transmembranei159 – 179HelicalSequence analysisAdd BLAST21
Topological domaini180 – 200CytoplasmicSequence analysisAdd BLAST21
Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
Topological domaini222 – 227LumenalSequence analysis6
Transmembranei228 – 248HelicalSequence analysisAdd BLAST21
Topological domaini249 – 278CytoplasmicSequence analysisAdd BLAST30
Transmembranei279 – 299HelicalSequence analysisAdd BLAST21
Topological domaini300 – 328LumenalSequence analysisAdd BLAST29
Transmembranei329 – 349HelicalSequence analysisAdd BLAST21
Topological domaini350 – 365CytoplasmicSequence analysisAdd BLAST16
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 391LumenalSequence analysis5
Transmembranei392 – 412HelicalSequence analysisAdd BLAST21
Topological domaini413 – 423CytoplasmicSequence analysisAdd BLAST11
Transmembranei424 – 444HelicalSequence analysisAdd BLAST21
Topological domaini445 – 457LumenalSequence analysisAdd BLAST13
Transmembranei458 – 478HelicalSequence analysisAdd BLAST21
Topological domaini479 – 495CytoplasmicSequence analysisAdd BLAST17

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • cytoplasm Source: MGI
  • cytoplasmic, membrane-bounded vesicle Source: MGI
  • integral component of membrane Source: GO_Central
  • lysosomal membrane Source: MGI
  • lysosome Source: GO_Central
  • plasma membrane Source: MGI
  • synaptic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002209481 – 495SialinAdd BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...)Sequence analysis1
Glycosylationi77N-linked (GlcNAc...)Sequence analysis1
Glycosylationi95N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8BN82.
PeptideAtlasiQ8BN82.
PRIDEiQ8BN82.

PTM databases

iPTMnetiQ8BN82.
PhosphoSitePlusiQ8BN82.

Expressioni

Gene expression databases

BgeeiENSMUSG00000049624.
GenevisibleiQ8BN82. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056182.

Structurei

3D structure databases

ProteinModelPortaliQ8BN82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi22 – 23Dileucine internalization motifBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230811.
HOVERGENiHBG008834.
InParanoidiQ8BN82.
KOiK12301.
OMAiRSSPNED.
OrthoDBiEOG091G06DG.
PhylomeDBiQ8BN82.
TreeFamiTF313535.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BN82-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRPLLRGPAG NDDEESSDST PLLPGARQTE AAPVCCSARY NLAILAFCGF
60 70 80 90 100
FVLYALRVNL SVALVDMVDS NTTLTDNRTS KECAEHSAPI KVHHNHTGKK
110 120 130 140 150
YKWDAETQGW ILGSFFYGYI VTQIPGGYIA SRVGGKLLLG LGILGTSVFT
160 170 180 190 200
LFTPLAADLG VVTLVVLRAL EGLGEGVTFP AMHAMWSSWA PPLERSKLLT
210 220 230 240 250
ISYAGAQLGT VISLPLSGII CYYMNWTYVF YLFGIVGIVW FILWMWIVSD
260 270 280 290 300
TPETHKTISH YEKEYIVSSL KNQLSSQKVV PWGSILKSLP LWAIVVAHFS
310 320 330 340 350
YNWSFYTLLT LLPTYMKEIL RFNVQENGFL SALPYFGCWL CMILCGQAAD
360 370 380 390 400
YLRVKWNFST ISVRRIFSLV GMVGPAVFLV AAGFIGCDYS LAVAFLTIST
410 420 430 440 450
TLGGFASSGF SINHLDIAPS YAGILLGITN TFATIPGMTG PIIAKSLTPD
460 470 480 490
NTIREWQTVF CIAAAINVFG AIFFTLFAKG EVQSWALSDH HGHRN
Length:495
Mass (Da):54,369
Last modified:June 7, 2004 - v2
Checksum:i6009661215D26437
GO
Isoform 2 (identifier: Q8BN82-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-123: Missing.

Note: No experimental confirmation available.
Show »
Length:469
Mass (Da):51,301
Checksum:iB48BCF204A2D6008
GO
Isoform 3 (identifier: Q8BN82-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     176-238: GVTFPAMHAM...VFYLFGIVGI → KYPPPGCYVS...YELDLRLLSF
     239-495: Missing.

Note: No experimental confirmation available.
Show »
Length:238
Mass (Da):25,879
Checksum:i95222793B0E39762
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01048498 – 123Missing in isoform 2. 1 PublicationAdd BLAST26
Alternative sequenceiVSP_010485176 – 238GVTFP…GIVGI → KYPPPGCYVSSYARHVVFLG SPSGKKQASYHFLCGSTAWD SDLTSSFRNNMLLYELDLRL LSF in isoform 3. 1 PublicationAdd BLAST63
Alternative sequenceiVSP_010486239 – 495Missing in isoform 3. 1 PublicationAdd BLAST257

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029102 mRNA. Translation: BAC26298.1.
AK087395 mRNA. Translation: BAC39859.1.
AK169868 mRNA. Translation: BAE41423.1.
BC058785 mRNA. Translation: AAH58785.1.
CCDSiCCDS23364.1. [Q8BN82-1]
CCDS72283.1. [Q8BN82-2]
RefSeqiNP_001263381.1. NM_001276452.1. [Q8BN82-2]
NP_766361.1. NM_172773.3. [Q8BN82-1]
UniGeneiMm.46932.

Genome annotation databases

EnsembliENSMUST00000052441; ENSMUSP00000056182; ENSMUSG00000049624. [Q8BN82-1]
ENSMUST00000117645; ENSMUSP00000113003; ENSMUSG00000049624. [Q8BN82-2]
ENSMUST00000119213; ENSMUSP00000113340; ENSMUSG00000049624. [Q8BN82-3]
GeneIDi235504.
KEGGimmu:235504.
UCSCiuc009quo.2. mouse. [Q8BN82-1]
uc009qup.2. mouse. [Q8BN82-3]
uc012gxg.2. mouse. [Q8BN82-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029102 mRNA. Translation: BAC26298.1.
AK087395 mRNA. Translation: BAC39859.1.
AK169868 mRNA. Translation: BAE41423.1.
BC058785 mRNA. Translation: AAH58785.1.
CCDSiCCDS23364.1. [Q8BN82-1]
CCDS72283.1. [Q8BN82-2]
RefSeqiNP_001263381.1. NM_001276452.1. [Q8BN82-2]
NP_766361.1. NM_172773.3. [Q8BN82-1]
UniGeneiMm.46932.

3D structure databases

ProteinModelPortaliQ8BN82.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000056182.

PTM databases

iPTMnetiQ8BN82.
PhosphoSitePlusiQ8BN82.

Proteomic databases

PaxDbiQ8BN82.
PeptideAtlasiQ8BN82.
PRIDEiQ8BN82.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052441; ENSMUSP00000056182; ENSMUSG00000049624. [Q8BN82-1]
ENSMUST00000117645; ENSMUSP00000113003; ENSMUSG00000049624. [Q8BN82-2]
ENSMUST00000119213; ENSMUSP00000113340; ENSMUSG00000049624. [Q8BN82-3]
GeneIDi235504.
KEGGimmu:235504.
UCSCiuc009quo.2. mouse. [Q8BN82-1]
uc009qup.2. mouse. [Q8BN82-3]
uc012gxg.2. mouse. [Q8BN82-2]

Organism-specific databases

CTDi26503.
MGIiMGI:1924105. Slc17a5.

Phylogenomic databases

eggNOGiKOG2532. Eukaryota.
ENOG410XPWC. LUCA.
GeneTreeiENSGT00760000119079.
HOGENOMiHOG000230811.
HOVERGENiHBG008834.
InParanoidiQ8BN82.
KOiK12301.
OMAiRSSPNED.
OrthoDBiEOG091G06DG.
PhylomeDBiQ8BN82.
TreeFamiTF313535.

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.
R-MMU-428643. Organic anion transporters.

Miscellaneous databases

ChiTaRSiSlc17a5. mouse.
PROiQ8BN82.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049624.
GenevisibleiQ8BN82. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS17A5_MOUSE
AccessioniPrimary (citable) accession number: Q8BN82
Secondary accession number(s): Q3TE25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.