Sialin - Mus musculus (Mouse)

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Q8BN82 (S17A5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 76. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sialin

Alternative name(s):
H(+)/nitrate cotransporter
H(+)/sialic acid cotransporter
Short name=AST
Solute carrier family 17 member 5
Vesicular H(+)/Aspartate-glutamate cotransporter

Gene names

Name:

Slc17a5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transports glucuronic acid and free sialic acid out of the lysosome after it is cleaved from sialoglycoconjugates undergoing degradation, this is required for normal CNS myelination. Mediates aspartate and glutamate membrane potential-dependent uptake into synaptic vesicles and synaptic-like microvesicles. Also functions as an electrogenic 2NO₃^-/H⁺ cotransporter in the plasma membrane of salivary gland acinar cells, mediating the physiological nitrate efflux, 25% of the circulating nitrate ions is typically removed and secreted in saliva By similarity. Ref.3
Subcellular location	Cell membrane By similarity; Multi-pass membrane protein. Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane By similarity. Lysosome membrane By similarity; Multi-pass membrane protein.
Sequence similarities	Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.

Ontologies

Keywords
Biological process	Amino-acid transport Symport Transport
Cellular component	Cell junction Cell membrane Cytoplasmic vesicle Lysosome Membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	amino acid transport Inferred from electronic annotation. Source: UniProtKB-KW sialic acid transport Inferred from electronic annotation. Source: Compara
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic membrane-bounded vesicle Inferred from direct assay PubMed 15006695. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 15006695. Source: MGI synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sialic acid transmembrane transporter activity Inferred from electronic annotation. Source: Compara symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BN82-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BN82-2) The sequence of this isoform differs from the canonical sequence as follows: 98-123: Missing.
Note: No experimental confirmation available.

Isoform 3 (identifier: Q8BN82-3) The sequence of this isoform differs from the canonical sequence as follows: 176-238: GVTFPAMHAM...VFYLFGIVGI → KYPPPGCYVS...YELDLRLLSF 239-495: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 495

495

Sialin

PRO_0000220948

Regions

Topological domain

1 – 41

Cytoplasmic Potential

Transmembrane

42 – 62

Helical; Potential

Topological domain

63 – 109

Lumenal Potential

Transmembrane

110 – 130

Helical; Potential

Topological domain

131 – 136

Cytoplasmic Potential

Transmembrane

137 – 157

Helical; Potential

Topological domain

158

Lumenal Potential

Transmembrane

159 – 179

Helical; Potential

Topological domain

180 – 200

Cytoplasmic Potential

Transmembrane

201 – 221

Helical; Potential

Topological domain

222 – 227

Lumenal Potential

Transmembrane

228 – 248

Helical; Potential

Topological domain

249 – 278

Cytoplasmic Potential

Transmembrane

279 – 299

Helical; Potential

Topological domain

300 – 328

Lumenal Potential

Transmembrane

329 – 349

Helical; Potential

Topological domain

350 – 365

Cytoplasmic Potential

Transmembrane

366 – 386

Helical; Potential

Topological domain

387 – 391

Lumenal Potential

Transmembrane

392 – 412

Helical; Potential

Topological domain

413 – 423

Cytoplasmic Potential

Transmembrane

424 – 444

Helical; Potential

Topological domain

445 – 457

Lumenal Potential

Transmembrane

458 – 478

Helical; Potential

Topological domain

479 – 495

Cytoplasmic Potential

Motif

22 – 23

Dileucine internalization motif By similarity

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence

98 – 123

Missing in isoform 2.

VSP_010484

Alternative sequence

176 – 238

GVTFP…GIVGI → KYPPPGCYVSSYARHVVFLG SPSGKKQASYHFLCGSTAWD SDLTSSFRNNMLLYELDLRL LSF in isoform 3.

VSP_010485

Alternative sequence

239 – 495

257

Missing in isoform 3.

VSP_010486

Sequences

Sequence		Length	Mass (Da)
Isoform 1 [UniParc]. Last modified June 7, 2004. Version 2. Checksum: 6009661215D26437 Show »	FASTA	495	54,369
10 20 30 40 50 60 MRPLLRGPAG NDDEESSDST PLLPGARQTE AAPVCCSARY NLAILAFCGF FVLYALRVNL 70 80 90 100 110 120 SVALVDMVDS NTTLTDNRTS KECAEHSAPI KVHHNHTGKK YKWDAETQGW ILGSFFYGYI 130 140 150 160 170 180 VTQIPGGYIA SRVGGKLLLG LGILGTSVFT LFTPLAADLG VVTLVVLRAL EGLGEGVTFP 190 200 210 220 230 240 AMHAMWSSWA PPLERSKLLT ISYAGAQLGT VISLPLSGII CYYMNWTYVF YLFGIVGIVW 250 260 270 280 290 300 FILWMWIVSD TPETHKTISH YEKEYIVSSL KNQLSSQKVV PWGSILKSLP LWAIVVAHFS 310 320 330 340 350 360 YNWSFYTLLT LLPTYMKEIL RFNVQENGFL SALPYFGCWL CMILCGQAAD YLRVKWNFST 370 380 390 400 410 420 ISVRRIFSLV GMVGPAVFLV AAGFIGCDYS LAVAFLTIST TLGGFASSGF SINHLDIAPS 430 440 450 460 470 480 YAGILLGITN TFATIPGMTG PIIAKSLTPD NTIREWQTVF CIAAAINVFG AIFFTLFAKG 490 EVQSWALSDH HGHRN « Hide
Isoform 2 [UniParc]. Checksum: B48BCF204A2D6008 Show »	FASTA	469	51,301
10 20 30 40 50 60 MRPLLRGPAG NDDEESSDST PLLPGARQTE AAPVCCSARY NLAILAFCGF FVLYALRVNL 70 80 90 100 110 120 SVALVDMVDS NTTLTDNRTS KECAEHSAPI KVHHNHTIPG GYIASRVGGK LLLGLGILGT 130 140 150 160 170 180 SVFTLFTPLA ADLGVVTLVV LRALEGLGEG VTFPAMHAMW SSWAPPLERS KLLTISYAGA 190 200 210 220 230 240 QLGTVISLPL SGIICYYMNW TYVFYLFGIV GIVWFILWMW IVSDTPETHK TISHYEKEYI 250 260 270 280 290 300 VSSLKNQLSS QKVVPWGSIL KSLPLWAIVV AHFSYNWSFY TLLTLLPTYM KEILRFNVQE 310 320 330 340 350 360 NGFLSALPYF GCWLCMILCG QAADYLRVKW NFSTISVRRI FSLVGMVGPA VFLVAAGFIG 370 380 390 400 410 420 CDYSLAVAFL TISTTLGGFA SSGFSINHLD IAPSYAGILL GITNTFATIP GMTGPIIAKS 430 440 450 460 LTPDNTIREW QTVFCIAAAI NVFGAIFFTL FAKGEVQSWA LSDHHGHRN « Hide
Isoform 3 [UniParc]. Checksum: 95222793B0E39762 Show »	FASTA	238	25,879
10 20 30 40 50 60 MRPLLRGPAG NDDEESSDST PLLPGARQTE AAPVCCSARY NLAILAFCGF FVLYALRVNL 70 80 90 100 110 120 SVALVDMVDS NTTLTDNRTS KECAEHSAPI KVHHNHTGKK YKWDAETQGW ILGSFFYGYI 130 140 150 160 170 180 VTQIPGGYIA SRVGGKLLLG LGILGTSVFT LFTPLAADLG VVTLVVLRAL EGLGEKYPPP 190 200 210 220 230 GCYVSSYARH VVFLGSPSGK KQASYHFLCG STAWDSDLTS SFRNNMLLYE LDLRLLSF « Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: C57BL/6J and NOD. Tissue: Eye and Skin.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Olfactory epithelium.
[3]	"The lysosomal sialic acid transporter sialin is required for normal CNS myelination." Prolo L.M., Vogel H., Reimer R.J. J. Neurosci. 29:15355-15365(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK029102 mRNA. Translation: BAC26298.1. AK087395 mRNA. Translation: BAC39859.1. AK169868 mRNA. Translation: BAE41423.1. BC058785 mRNA. Translation: AAH58785.1.
IPI	IPI00264331. IPI00407283. IPI00416350.
RefSeq	NP_001263381.1. NM_001276452.1. NP_766361.1. NM_172773.3.
UniGene	Mm.46932.
3D structure databases
ProteinModelPortal	Q8BN82.
ModBase	Search...
PTM databases
PhosphoSite	Q8BN82.
Proteomic databases
PRIDE	Q8BN82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000052441; ENSMUSP00000056182; ENSMUSG00000049624. ENSMUST00000117645; ENSMUSP00000113003; ENSMUSG00000049624. ENSMUST00000119213; ENSMUSP00000113340; ENSMUSG00000049624.
GeneID	235504.
KEGG	mmu:235504.
UCSC	uc009quo.1. mouse. uc009qup.1. mouse. uc012gxg.1. mouse.
Organism-specific databases
CTD	26503.
MGI	MGI:1924105. Slc17a5.
Phylogenomic databases
eggNOG	COG0477.
GeneTree	ENSGT00640000091442.
HOGENOM	HOG000230811.
HOVERGEN	HBG008834.
InParanoid	Q8BN82.
KO	K12301.
OMA	MHFIANI.
OrthoDB	EOG4640BQ.
Gene expression databases
Bgee	Q8BN82.
Genevestigator	Q8BN82.
GermOnline	ENSMUSG00000049624. Mus musculus.
Family and domain databases
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SLC17A5. mouse.
NextBio	382720.
SOURCE	Search...

Entry information

Entry name

S17A5_MOUSE

Accession

Primary (citable) accession number: Q8BN82
Secondary accession number(s): Q3TE25

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents