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Q8BN78 (KAISO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcriptional regulator Kaiso
Alternative name(s):
Zinc finger and BTB domain-containing protein 33
Gene names
Name:Zbtb33
Synonyms:Kaiso
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length671 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. May recruit the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Contributes to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Ref.4 Ref.6 Ref.7 Ref.8

Subunit structure

Interacts with NCOR1 By similarity. Self-associates. Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA. Interacts with CTNND2. Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein. Ref.1 Ref.3 Ref.6 Ref.7

Subcellular location

Nucleus Ref.1 Ref.6 Ref.7.

Tissue specificity

Expressed in brain, heart, kidney, liver, lung, neuromuscular junctions, skeletal muscle, spleen and testis. Ref.1 Ref.7

Sequence similarities

Contains 1 BTB (POZ) domain.

Contains 3 C2H2-type zinc fingers.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ctnnd1P309993EBI-1216314,EBI-529924

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 671671Transcriptional regulator Kaiso
PRO_0000046989

Regions

Domain32 – 9463BTB
Zinc finger492 – 51423C2H2-type 1
Zinc finger520 – 54223C2H2-type 2
Zinc finger548 – 57124C2H2-type 3
Region1 – 136136Self-association
Region1 – 103103Interaction with NCOR1 By similarity
Region452 – 671220Interaction with CTNND1
Region512 – 637126Required for DNA-binding
Motif469 – 47810Nuclear localization signal Ref.6

Experimental info

Mutagenesis4721K → A: Abrogates nuclear localization. Ref.6
Sequence conflict4411D → N in AAD20989. Ref.1
Sequence conflict4511D → H in AAD20989. Ref.1
Sequence conflict6401E → K in AAD20989. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8BN78 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: CFD23E0C15B491D1

FASTA67174,050
        10         20         30         40         50         60 
MESRKLISAT DIQYSASLLN SLNEQRGHGL FCDVTVIVED RKFRAHRNIL SASSTYFHQL 

        70         80         90        100        110        120 
FSVAGQVVEL SFIRAEIFAE ILNYIYSSKV VRVRADLLDE LIKSGQLLGV KFIAELGVPL 

       130        140        150        160        170        180 
SQVKSISGTE QDGTAETLPS SSSDKSLDME KSKDEAQDNG ATVMPIITES FSLSAEDNEM 

       190        200        210        220        230        240 
KKIIVTDSDD DDDDDVIFCS EILPAKEDLP SNNTATQVQP NPASVAISEV TPCASNNSPP 

       250        260        270        280        290        300 
VTNITPTQLP TPVNQATLSQ TQGSEELLVS SASTHLTPNI ILLNQAPLTA PPSASSSLPN 

       310        320        330        340        350        360 
HMSSSVNVLV QNQQTPNSAV LTGNKAEEEE EIIDDDDDII SSSPDSAVSN TSLVPQADNS 

       370        380        390        400        410        420 
KSTTLDGSLT QKMQIPVLPQ EPPSNSLKIS DVITRNTNDP GLRSKHVMEG QKIITLDTAT 

       430        440        450        460        470        480 
EIEGLSTGCK VYANIGEDTY DIVIPVKDDP DGGEAKLDNE LPKTSGSEPP NKRMKVKHDD 

       490        500        510        520        530        540 
HYELIVDGRV YYICIVCKRS YVCLTSLRRH FNIHSWEKKY QCRYCDKVFP LAEYRTKHEI 

       550        560        570        580        590        600 
HHTGERRYQC LTCGKSFINY QFMSSHIKSV HSQDPSGDSK LYRLHPCKSL QIRQYAYLSN 

       610        620        630        640        650        660 
KSSAMPVMKD DAVGYKVDAG KEPPVGTTST PPQNKSTFWE DIFIQQENDS IFKQNVTDGS 

       670 
TEFEFIIPES Y 

« Hide

References

« Hide 'large scale' references
[1]"The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor."
Daniel J.M., Reynolds A.B.
Mol. Cell. Biol. 19:3614-3623(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SELF-ASSOCIATION, INTERACTION WITH CTNND1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Eye.
[3]"The p120(ctn)-binding partner Kaiso is a bi-modal DNA-binding protein that recognizes both a sequence-specific consensus and methylated CpG dinucleotides."
Daniel J.M., Spring C.M., Crawford H.C., Reynolds A.B., Baig A.
Nucleic Acids Res. 30:2911-2919(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING, INTERACTION WITH CTNND1.
[4]"NLS-dependent nuclear localization of p120ctn is necessary to relieve Kaiso-mediated transcriptional repression."
Kelly K.F., Spring C.M., Otchere A.A., Daniel J.M.
J. Cell Sci. 117:2675-2686(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]Erratum
Kelly K.F., Spring C.M., Otchere A.A., Daniel J.M.
J. Cell Sci. 117:3405-3405(2004)
[6]"Nuclear import of the BTB/POZ transcriptional regulator Kaiso."
Kelly K.F., Otchere A.A., Graham M., Daniel J.M.
J. Cell Sci. 117:6143-6152(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH KPNA2, SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, MUTAGENESIS OF LYS-472.
[7]"Regulation of the rapsyn promoter by kaiso and delta-catenin."
Rodova M., Kelly K.F., VanSaun M., Daniel J.M., Werle M.J.
Mol. Cell. Biol. 24:7188-7196(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CTNND2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[8]"The catenin p120ctn inhibits Kaiso-mediated transcriptional repression of the beta-catenin/TCF target gene matrilysin."
Spring C.M., Kelly K.F., O'Kelly I., Graham M., Crawford H.C., Daniel J.M.
Exp. Cell Res. 305:253-265(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"The tissue-specific methylation of the human tyrosine hydroxylase gene reveals new regulatory elements in the first exon."
Aranyi T., Faucheux B.A., Khalfallah O., Vodjdani G., Biguet N.F., Mallet J., Meloni R.
J. Neurochem. 94:129-139(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF097416 mRNA. Translation: AAD20989.1.
AK087417 mRNA. Translation: BAC39866.1.
AK089423 mRNA. Translation: BAC40875.1.
RefSeqNP_001072981.1. NM_001079513.1.
NP_064652.2. NM_020256.2.
UniGeneMm.391248.

3D structure databases

ProteinModelPortalQ8BN78.
SMRQ8BN78. Positions 3-117, 406-604.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208181. 6 interactions.
IntActQ8BN78. 2 interactions.
MINTMINT-89891.

PTM databases

PhosphoSiteQ8BN78.

Proteomic databases

PRIDEQ8BN78.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049740; ENSMUSP00000049983; ENSMUSG00000048047.
ENSMUST00000115142; ENSMUSP00000110795; ENSMUSG00000048047.
GeneID56805.
KEGGmmu:56805.
UCSCuc009szp.1. mouse.

Organism-specific databases

CTD10009.
MGIMGI:1927290. Zbtb33.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00530000063209.
HOGENOMHOG000230932.
HOVERGENHBG079575.
InParanoidQ8BN78.
KOK10507.
OMAHSQDPSG.
OrthoDBEOG7WX087.
PhylomeDBQ8BN78.
TreeFamTF333100.

Gene expression databases

ArrayExpressQ8BN78.
BgeeQ8BN78.
CleanExMM_ZBTB33.
GenevestigatorQ8BN78.

Family and domain databases

Gene3D3.30.160.60. 3 hits.
3.30.710.10. 1 hit.
InterProIPR000210. BTB/POZ-like.
IPR011333. BTB/POZ_fold.
IPR013069. BTB_POZ.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamPF00651. BTB. 1 hit.
[Graphical view]
SMARTSM00225. BTB. 1 hit.
SM00355. ZnF_C2H2. 3 hits.
[Graphical view]
SUPFAMSSF54695. SSF54695. 1 hit.
PROSITEPS50097. BTB. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZBTB33. mouse.
NextBio313351.
PROQ8BN78.
SOURCESearch...

Entry information

Entry nameKAISO_MOUSE
AccessionPrimary (citable) accession number: Q8BN78
Secondary accession number(s): Q8C226, Q9WTZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot