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Q8BN78

- KAISO_MOUSE

UniProt

Q8BN78 - KAISO_MOUSE

Protein

Transcriptional regulator Kaiso

Gene

Zbtb33

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. May recruit the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Contributes to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri492 – 51423C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri520 – 54223C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri548 – 57124C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: MGI
    3. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation, Wnt signaling pathway

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional regulator Kaiso
    Alternative name(s):
    Zinc finger and BTB domain-containing protein 33
    Gene namesi
    Name:Zbtb33
    Synonyms:Kaiso
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1927290. Zbtb33.

    Subcellular locationi

    Nucleus 3 Publications

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleolus Source: Ensembl
    3. nucleus Source: MGI
    4. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi472 – 4721K → A: Abrogates nuclear localization. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 671671Transcriptional regulator KaisoPRO_0000046989Add
    BLAST

    Proteomic databases

    PRIDEiQ8BN78.

    PTM databases

    PhosphoSiteiQ8BN78.

    Expressioni

    Tissue specificityi

    Expressed in brain, heart, kidney, liver, lung, neuromuscular junctions, skeletal muscle, spleen and testis.2 Publications

    Gene expression databases

    ArrayExpressiQ8BN78.
    BgeeiQ8BN78.
    CleanExiMM_ZBTB33.
    GenevestigatoriQ8BN78.

    Interactioni

    Subunit structurei

    Interacts with NCOR1 By similarity. Self-associates. Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA. Interacts with CTNND2. Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Ctnnd1P309993EBI-1216314,EBI-529924

    Protein-protein interaction databases

    BioGridi208181. 6 interactions.
    IntActiQ8BN78. 2 interactions.
    MINTiMINT-89891.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BN78.
    SMRiQ8BN78. Positions 3-117, 479-598.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 9463BTBPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 136136Self-associationAdd
    BLAST
    Regioni1 – 103103Interaction with NCOR1By similarityAdd
    BLAST
    Regioni452 – 671220Interaction with CTNND1Add
    BLAST
    Regioni512 – 637126Required for DNA-bindingAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi469 – 47810Nuclear localization signal1 Publication

    Sequence similaritiesi

    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
    Contains 3 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri492 – 51423C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri520 – 54223C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri548 – 57124C2H2-type 3PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00530000063209.
    HOGENOMiHOG000230932.
    HOVERGENiHBG079575.
    InParanoidiQ8BN78.
    KOiK10507.
    OMAiHSQDPSG.
    OrthoDBiEOG7WX087.
    PhylomeDBiQ8BN78.
    TreeFamiTF333100.

    Family and domain databases

    Gene3Di3.30.160.60. 3 hits.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    PfamiPF00651. BTB. 1 hit.
    [Graphical view]
    SMARTiSM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 3 hits.
    [Graphical view]
    SUPFAMiSSF54695. SSF54695. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8BN78-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESRKLISAT DIQYSASLLN SLNEQRGHGL FCDVTVIVED RKFRAHRNIL    50
    SASSTYFHQL FSVAGQVVEL SFIRAEIFAE ILNYIYSSKV VRVRADLLDE 100
    LIKSGQLLGV KFIAELGVPL SQVKSISGTE QDGTAETLPS SSSDKSLDME 150
    KSKDEAQDNG ATVMPIITES FSLSAEDNEM KKIIVTDSDD DDDDDVIFCS 200
    EILPAKEDLP SNNTATQVQP NPASVAISEV TPCASNNSPP VTNITPTQLP 250
    TPVNQATLSQ TQGSEELLVS SASTHLTPNI ILLNQAPLTA PPSASSSLPN 300
    HMSSSVNVLV QNQQTPNSAV LTGNKAEEEE EIIDDDDDII SSSPDSAVSN 350
    TSLVPQADNS KSTTLDGSLT QKMQIPVLPQ EPPSNSLKIS DVITRNTNDP 400
    GLRSKHVMEG QKIITLDTAT EIEGLSTGCK VYANIGEDTY DIVIPVKDDP 450
    DGGEAKLDNE LPKTSGSEPP NKRMKVKHDD HYELIVDGRV YYICIVCKRS 500
    YVCLTSLRRH FNIHSWEKKY QCRYCDKVFP LAEYRTKHEI HHTGERRYQC 550
    LTCGKSFINY QFMSSHIKSV HSQDPSGDSK LYRLHPCKSL QIRQYAYLSN 600
    KSSAMPVMKD DAVGYKVDAG KEPPVGTTST PPQNKSTFWE DIFIQQENDS 650
    IFKQNVTDGS TEFEFIIPES Y 671
    Length:671
    Mass (Da):74,050
    Last modified:March 1, 2003 - v1
    Checksum:iCFD23E0C15B491D1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti441 – 4411D → N in AAD20989. (PubMed:10207085)Curated
    Sequence conflicti451 – 4511D → H in AAD20989. (PubMed:10207085)Curated
    Sequence conflicti640 – 6401E → K in AAD20989. (PubMed:10207085)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF097416 mRNA. Translation: AAD20989.1.
    AK087417 mRNA. Translation: BAC39866.1.
    AK089423 mRNA. Translation: BAC40875.1.
    CCDSiCCDS30088.1.
    RefSeqiNP_001072981.1. NM_001079513.1.
    NP_064652.2. NM_020256.2.
    UniGeneiMm.391248.

    Genome annotation databases

    EnsembliENSMUST00000049740; ENSMUSP00000049983; ENSMUSG00000048047.
    ENSMUST00000115142; ENSMUSP00000110795; ENSMUSG00000048047.
    GeneIDi56805.
    KEGGimmu:56805.
    UCSCiuc009szp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF097416 mRNA. Translation: AAD20989.1 .
    AK087417 mRNA. Translation: BAC39866.1 .
    AK089423 mRNA. Translation: BAC40875.1 .
    CCDSi CCDS30088.1.
    RefSeqi NP_001072981.1. NM_001079513.1.
    NP_064652.2. NM_020256.2.
    UniGenei Mm.391248.

    3D structure databases

    ProteinModelPortali Q8BN78.
    SMRi Q8BN78. Positions 3-117, 479-598.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208181. 6 interactions.
    IntActi Q8BN78. 2 interactions.
    MINTi MINT-89891.

    PTM databases

    PhosphoSitei Q8BN78.

    Proteomic databases

    PRIDEi Q8BN78.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000049740 ; ENSMUSP00000049983 ; ENSMUSG00000048047 .
    ENSMUST00000115142 ; ENSMUSP00000110795 ; ENSMUSG00000048047 .
    GeneIDi 56805.
    KEGGi mmu:56805.
    UCSCi uc009szp.1. mouse.

    Organism-specific databases

    CTDi 10009.
    MGIi MGI:1927290. Zbtb33.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00530000063209.
    HOGENOMi HOG000230932.
    HOVERGENi HBG079575.
    InParanoidi Q8BN78.
    KOi K10507.
    OMAi HSQDPSG.
    OrthoDBi EOG7WX087.
    PhylomeDBi Q8BN78.
    TreeFami TF333100.

    Miscellaneous databases

    ChiTaRSi ZBTB33. mouse.
    NextBioi 313351.
    PROi Q8BN78.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8BN78.
    Bgeei Q8BN78.
    CleanExi MM_ZBTB33.
    Genevestigatori Q8BN78.

    Family and domain databases

    Gene3Di 3.30.160.60. 3 hits.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    Pfami PF00651. BTB. 1 hit.
    [Graphical view ]
    SMARTi SM00225. BTB. 1 hit.
    SM00355. ZnF_C2H2. 3 hits.
    [Graphical view ]
    SUPFAMi SSF54695. SSF54695. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    PS00028. ZINC_FINGER_C2H2_1. 3 hits.
    PS50157. ZINC_FINGER_C2H2_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The catenin p120(ctn) interacts with Kaiso, a novel BTB/POZ domain zinc finger transcription factor."
      Daniel J.M., Reynolds A.B.
      Mol. Cell. Biol. 19:3614-3623(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SELF-ASSOCIATION, INTERACTION WITH CTNND1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Eye.
    3. "The p120(ctn)-binding partner Kaiso is a bi-modal DNA-binding protein that recognizes both a sequence-specific consensus and methylated CpG dinucleotides."
      Daniel J.M., Spring C.M., Crawford H.C., Reynolds A.B., Baig A.
      Nucleic Acids Res. 30:2911-2919(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING, INTERACTION WITH CTNND1.
    4. "NLS-dependent nuclear localization of p120ctn is necessary to relieve Kaiso-mediated transcriptional repression."
      Kelly K.F., Spring C.M., Otchere A.A., Daniel J.M.
      J. Cell Sci. 117:2675-2686(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. Erratum
      Kelly K.F., Spring C.M., Otchere A.A., Daniel J.M.
      J. Cell Sci. 117:3405-3405(2004)
    6. "Nuclear import of the BTB/POZ transcriptional regulator Kaiso."
      Kelly K.F., Otchere A.A., Graham M., Daniel J.M.
      J. Cell Sci. 117:6143-6152(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH KPNA2, SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, MUTAGENESIS OF LYS-472.
    7. "Regulation of the rapsyn promoter by kaiso and delta-catenin."
      Rodova M., Kelly K.F., VanSaun M., Daniel J.M., Werle M.J.
      Mol. Cell. Biol. 24:7188-7196(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH CTNND2, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    8. "The catenin p120ctn inhibits Kaiso-mediated transcriptional repression of the beta-catenin/TCF target gene matrilysin."
      Spring C.M., Kelly K.F., O'Kelly I., Graham M., Crawford H.C., Daniel J.M.
      Exp. Cell Res. 305:253-265(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "The tissue-specific methylation of the human tyrosine hydroxylase gene reveals new regulatory elements in the first exon."
      Aranyi T., Faucheux B.A., Khalfallah O., Vodjdani G., Biguet N.F., Mallet J., Meloni R.
      J. Neurochem. 94:129-139(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING.

    Entry informationi

    Entry nameiKAISO_MOUSE
    AccessioniPrimary (citable) accession number: Q8BN78
    Secondary accession number(s): Q8C226, Q9WTZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3