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Q8BN21 (VRK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase VRK2

EC=2.7.11.1
Alternative name(s):
Vaccinia-related kinase 2
Gene names
Name:Vrk2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta (IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase (MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates histone H3. Phosphorylates 'Thr-18' of p53/TP53, and thereby increases its stability and activity. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. May also phosphorylate MAPK8IP1. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant By similarity. Ref.6

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with MAP3K7, MAP2K7, MAP2K1, KSR1, RAN and MAPK8IP1 By similarity.

Subcellular location

Cytoplasm. Endoplasmic reticulum membrane; Single-pass type IV membrane protein Potential. Mitochondrion membrane; Single-pass type IV membrane protein Potential Ref.6.

Tissue specificity

Expressed in liver, kidney and muscle. Weakly expressed in thymus, bone marrow and spleen. Ref.5

Developmental stage

Weakly expressed in embryo compared to VRK1 and VRK3. Expressed from E10.5 to E14 in developing liver and then decreases. It increases again from E17.5 and remains thereafter. Highly expressed in hematopoietic embryonic tissues from E10.5 to E14.5. Weakly expressed in the yolk-sac. Ref.5

Post-translational modification

Autophosphorylated. Ref.6

Sequence similarities

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Endoplasmic reticulum
Membrane
Mitochondrion
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to oxidative stress

Inferred from sequence or structural similarity. Source: UniProtKB

protein autophosphorylation

Inferred from direct assay Ref.6. Source: MGI

protein phosphorylation

Inferred from direct assay Ref.6. Source: MGI

regulation of MAPK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of interleukin-1-mediated signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

endoplasmic reticulum

Inferred from direct assay Ref.6. Source: MGI

endoplasmic reticulum membrane

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from direct assay Ref.6. Source: MGI

mitochondrial membrane

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine kinase activity

Inferred from direct assay Ref.6. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8BN21-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8BN21-2)

The sequence of this isoform differs from the canonical sequence as follows:
     342-390: Missing.
Note: No experimental confirmation available.
Isoform 3 (identifier: Q8BN21-3)

The sequence of this isoform differs from the canonical sequence as follows:
     151-163: LDVLEYIHENEYV → VSLRDLTGDLLDI
     164-503: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Serine/threonine-protein kinase VRK2
PRO_0000086807

Regions

Transmembrane482 – 50221Helical; Anchor for type IV membrane protein; Potential
Domain29 – 313285Protein kinase
Nucleotide binding35 – 439ATP By similarity
Region392 – 503112Interaction with MAP3K7 By similarity

Sites

Active site1661Proton acceptor By similarity
Binding site611ATP By similarity

Amino acid modifications

Modified residue3361Phosphothreonine By similarity

Natural variations

Alternative sequence151 – 16313LDVLE…ENEYV → VSLRDLTGDLLDI in isoform 3.
VSP_008541
Alternative sequence164 – 503340Missing in isoform 3.
VSP_008542
Alternative sequence342 – 39049Missing in isoform 2.
VSP_008543

Experimental info

Sequence conflict331K → R in AAN64922. Ref.1
Sequence conflict2261A → P in BAB28393. Ref.2
Sequence conflict3191P → L in BAE32620. Ref.2
Sequence conflict3191P → L in AAH13520. Ref.4
Sequence conflict3331H → R in BAE32620. Ref.2
Sequence conflict3331H → R in AAH13520. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 10, 2003. Version 2.
Checksum: 6F29E3E412ECB221

FASTA50358,119
        10         20         30         40         50         60 
MAPRRKEKYK LPVPLPEGKI LDDMEGNRWA LGKMIGSGGF GLIYLAFPTN KPNKDARHVI 

        70         80         90        100        110        120 
KLEYQENGPL FSELKFYQRA AKRECIQKWI QQRKLDYLGI PVFYGFGLTD FKGRSYRFMV 

       130        140        150        160        170        180 
MERLGIDLQK LLDQNGGFKK LTVLQLGIRM LDVLEYIHEN EYVHGDIKAA NLLLDFTNPD 

       190        200        210        220        230        240 
RVYLADYGLS YRYCPNGNHK QYQEDPRKGH NGTIEFTSLD AHKGVAPSRR SDVEILGYCM 

       250        260        270        280        290        300 
LHWLFGKLPW EAKLDDPVAV QTAKTNLLDE LPESVLKWAP SGSSCSELVK YLMYVHNLAY 

       310        320        330        340        350        360 
DDKPDYQKLK KILNPDGVPL GPLEFSTKVQ SVHVRTPAQQ KVDSPKATRK PANEFPAKFP 

       370        380        390        400        410        420 
KKVHRETRAR QREEQEDSQP TMLQSRPAAP ENSRTRKIHE YSDIFSEMQS LQQTPSYMSF 

       430        440        450        460        470        480 
QGSYCKPYLD CTRRDPIRKP RSLPRYRHTP TGNLGVTDLE SSPRFWPAIF QLTLSEETKA 

       490        500 
DVYYYGITIF CLLIFVFLAL YFL 

« Hide

Isoform 2 [UniParc].

Checksum: 4AAD5406E8214578
Show »

FASTA45452,493
Isoform 3 [UniParc].

Checksum: 43B728DB7DC2FE52
Show »

FASTA16318,844

References

« Hide 'large scale' references
[1]"A novel gene, Pog, is necessary for primordial germ cell proliferation in the mouse and underlies the germ cell deficient mutation, gcd."
Agoulnik A.I., Lu B., Zhu Q., Truong C., Ty M.T., Arango N., Chada K.K., Bishop C.E.
Hum. Mol. Genet. 11:3047-3053(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Strain: C57BL/6J and NOD.
Tissue: Dendritic cell, Embryo, Eye and Spleen.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Kidney.
[5]"Expression of the VRK (vaccinia-related kinase) gene family of p53 regulators in murine hematopoietic development."
Vega F.M., Gonzalo P., Gaspar M.L., Lazo P.A.
FEBS Lett. 544:176-180(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[6]"Characterization of three paralogous members of the Mammalian vaccinia related kinase family."
Nichols R.J., Traktman P.
J. Biol. Chem. 279:7934-7946(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF513620 mRNA. Translation: AAN64922.1.
AK012664 mRNA. Translation: BAB28393.1.
AK053297 mRNA. Translation: BAC35335.1.
AK154485 mRNA. Translation: BAE32620.1.
AL929272, AC083815 Genomic DNA. Translation: CAI52021.1.
BC013520 mRNA. Translation: AAH13520.1.
RefSeqNP_001239376.1. NM_001252447.1.
NP_081536.2. NM_027260.3.
XP_006514864.1. XM_006514801.1.
XP_006514865.1. XM_006514802.1.
UniGeneMm.285351.

3D structure databases

ProteinModelPortalQ8BN21.
SMRQ8BN21. Positions 15-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid213758. 1 interaction.

PTM databases

PhosphoSiteQ8BN21.

Proteomic databases

PRIDEQ8BN21.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000078362; ENSMUSP00000077471; ENSMUSG00000064090. [Q8BN21-1]
ENSMUST00000109504; ENSMUSP00000105130; ENSMUSG00000064090. [Q8BN21-1]
GeneID69922.
KEGGmmu:69922.
UCSCuc007igf.2. mouse. [Q8BN21-1]
uc007igg.2. mouse. [Q8BN21-3]
uc011xsk.2. mouse. [Q8BN21-2]

Organism-specific databases

CTD7444.
MGIMGI:1917172. Vrk2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00730000110259.
HOVERGENHBG007532.
InParanoidQ8BN21.
KOK08816.
OMAHQDFTSP.
OrthoDBEOG7KSX83.
PhylomeDBQ8BN21.
TreeFamTF106473.

Gene expression databases

ArrayExpressQ8BN21.
BgeeQ8BN21.
CleanExMM_VRK2.
GenevestigatorQ8BN21.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio330615.
PROQ8BN21.
SOURCESearch...

Entry information

Entry nameVRK2_MOUSE
AccessionPrimary (citable) accession number: Q8BN21
Secondary accession number(s): Q3U415 expand/collapse secondary AC list , Q5F1Z5, Q5SP88, Q8BPU8, Q8CJ46, Q91WS1, Q9CZF9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 10, 2003
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot