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Q8BMZ5

- SEN34_MOUSE

UniProt

Q8BMZ5 - SEN34_MOUSE

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Protein

tRNA-splicing endonuclease subunit Sen34

Gene
Tsen34, Leng5, Sen34
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei253 – 2531 By similarity
Active sitei261 – 2611 By similarity
Active sitei292 – 2921 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. protein binding Source: MGI
  4. tRNA-intron endonuclease activity Source: InterPro

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. tRNA-type intron splice site recognition and cleavage Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

mRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen34 (EC:4.6.1.16)
Alternative name(s):
Leukocyte receptor cluster member 5 homolog
tRNA-intron endonuclease Sen34
Gene namesi
Name:Tsen34
Synonyms:Leng5, Sen34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1913328. Tsen34.

Subcellular locationi

Nucleus By similarity. Nucleusnucleolus By similarity
Note: May be transiently localized in the nucleolus By similarity.

GO - Cellular componenti

  1. nucleolus Source: UniProtKB-SubCell
  2. tRNA-intron endonuclease complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316tRNA-splicing endonuclease subunit Sen34PRO_0000109464Add
BLAST

Proteomic databases

PaxDbiQ8BMZ5.
PRIDEiQ8BMZ5.

PTM databases

PhosphoSiteiQ8BMZ5.

Expressioni

Gene expression databases

BgeeiQ8BMZ5.
GenevestigatoriQ8BMZ5.

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 By similarity.

Protein-protein interaction databases

IntActiQ8BMZ5. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ8BMZ5.
SMRiQ8BMZ5. Positions 203-312.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
GeneTreeiENSGT00390000003912.
HOGENOMiHOG000232197.
HOVERGENiHBG054846.
InParanoidiQ8BMZ5.
KOiK15323.
OMAiRTFSIEW.
OrthoDBiEOG75XGMQ.
PhylomeDBiQ8BMZ5.
TreeFamiTF314631.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR016690. tRNA_splic_SEN34.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8BMZ5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MLVVEVANGR SLVWGAEAVQ ALRERLGVGG RTVGALPRGP RQNSRLGLPL    50
LLLPEEARLL AEIGAVTLVS APRPDPRNHG LALASFKRQQ EQSFQDQNTL 100
AAEARETRRQ ELLEKIVEGQ AAKKQKLEQD SGADEGGQEA GGSEATQGSE 150
TSDDGQPSAE QEGAAPSLDS SSPQPGPSNG VTPLPRSALL IQLATARPRP 200
VKAKPLDWRV QSKDWPHAGR PAHELRYSIY RDLWERGFFL SAAGKFGGDF 250
LVYPGDPLRF HAHYIAQCWS AEDPIPLQDL VSAGRLGTSV RKTLLLCSPQ 300
PDGKVVYTSL QWASLQ 316
Length:316
Mass (Da):34,196
Last modified:July 19, 2004 - v2
Checksum:i86A169BC119F7AC3
GO
Isoform 2 (identifier: Q8BMZ5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-283: Missing.

Note: No experimental confirmation available.

Show »
Length:287
Mass (Da):30,964
Checksum:i0692A1E911014158
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei255 – 28329Missing in isoform 2. VSP_010987Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti20 – 201Q → L in BAB28747. 1 Publication
Sequence conflicti280 – 2801L → Q in BAB28747. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK003757 mRNA. Translation: BAC25051.1.
AK013253 mRNA. Translation: BAB28747.1.
AK053916 mRNA. Translation: BAC35591.1.
BC002205 mRNA. Translation: AAH02205.1.
BC091756 mRNA. Translation: AAH91756.1.
CCDSiCCDS20725.1. [Q8BMZ5-1]
CCDS51966.1. [Q8BMZ5-2]
RefSeqiNP_001157676.1. NM_001164204.1. [Q8BMZ5-1]
NP_001157677.1. NM_001164205.1. [Q8BMZ5-2]
NP_077130.1. NM_024168.2. [Q8BMZ5-1]
XP_006540345.1. XM_006540282.1. [Q8BMZ5-1]
XP_006540346.1. XM_006540283.1. [Q8BMZ5-1]
XP_006540347.1. XM_006540284.1. [Q8BMZ5-1]
XP_006540348.1. XM_006540285.1. [Q8BMZ5-1]
UniGeneiMm.379100.

Genome annotation databases

EnsembliENSMUST00000108627; ENSMUSP00000104267; ENSMUSG00000035585. [Q8BMZ5-1]
ENSMUST00000108629; ENSMUSP00000104269; ENSMUSG00000035585. [Q8BMZ5-2]
ENSMUST00000108630; ENSMUSP00000104270; ENSMUSG00000035585. [Q8BMZ5-1]
GeneIDi66078.
KEGGimmu:66078.
UCSCiuc009evu.2. mouse. [Q8BMZ5-2]
uc009evv.2. mouse. [Q8BMZ5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK003757 mRNA. Translation: BAC25051.1 .
AK013253 mRNA. Translation: BAB28747.1 .
AK053916 mRNA. Translation: BAC35591.1 .
BC002205 mRNA. Translation: AAH02205.1 .
BC091756 mRNA. Translation: AAH91756.1 .
CCDSi CCDS20725.1. [Q8BMZ5-1 ]
CCDS51966.1. [Q8BMZ5-2 ]
RefSeqi NP_001157676.1. NM_001164204.1. [Q8BMZ5-1 ]
NP_001157677.1. NM_001164205.1. [Q8BMZ5-2 ]
NP_077130.1. NM_024168.2. [Q8BMZ5-1 ]
XP_006540345.1. XM_006540282.1. [Q8BMZ5-1 ]
XP_006540346.1. XM_006540283.1. [Q8BMZ5-1 ]
XP_006540347.1. XM_006540284.1. [Q8BMZ5-1 ]
XP_006540348.1. XM_006540285.1. [Q8BMZ5-1 ]
UniGenei Mm.379100.

3D structure databases

ProteinModelPortali Q8BMZ5.
SMRi Q8BMZ5. Positions 203-312.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8BMZ5. 1 interaction.

PTM databases

PhosphoSitei Q8BMZ5.

Proteomic databases

PaxDbi Q8BMZ5.
PRIDEi Q8BMZ5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000108627 ; ENSMUSP00000104267 ; ENSMUSG00000035585 . [Q8BMZ5-1 ]
ENSMUST00000108629 ; ENSMUSP00000104269 ; ENSMUSG00000035585 . [Q8BMZ5-2 ]
ENSMUST00000108630 ; ENSMUSP00000104270 ; ENSMUSG00000035585 . [Q8BMZ5-1 ]
GeneIDi 66078.
KEGGi mmu:66078.
UCSCi uc009evu.2. mouse. [Q8BMZ5-2 ]
uc009evv.2. mouse. [Q8BMZ5-1 ]

Organism-specific databases

CTDi 79042.
MGIi MGI:1913328. Tsen34.

Phylogenomic databases

eggNOGi COG1676.
GeneTreei ENSGT00390000003912.
HOGENOMi HOG000232197.
HOVERGENi HBG054846.
InParanoidi Q8BMZ5.
KOi K15323.
OMAi RTFSIEW.
OrthoDBi EOG75XGMQ.
PhylomeDBi Q8BMZ5.
TreeFami TF314631.

Miscellaneous databases

NextBioi 320564.
PROi Q8BMZ5.
SOURCEi Search...

Gene expression databases

Bgeei Q8BMZ5.
Genevestigatori Q8BMZ5.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR016690. tRNA_splic_SEN34.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
[Graphical view ]
PIRSFi PIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Embryo and Oviduct.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Salivary gland.

Entry informationi

Entry nameiSEN34_MOUSE
AccessioniPrimary (citable) accession number: Q8BMZ5
Secondary accession number(s): Q58EU2, Q99LV6, Q9CYW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: July 9, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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