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Q8BMZ5

- SEN34_MOUSE

UniProt

Q8BMZ5 - SEN34_MOUSE

Protein

tRNA-splicing endonuclease subunit Sen34

Gene

Tsen34

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei253 – 2531By similarity
    Active sitei261 – 2611By similarity
    Active sitei292 – 2921By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. protein binding Source: MGI
    4. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. tRNA-type intron splice site recognition and cleavage Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    mRNA processing, tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonuclease subunit Sen34 (EC:4.6.1.16)
    Alternative name(s):
    Leukocyte receptor cluster member 5 homolog
    tRNA-intron endonuclease Sen34
    Gene namesi
    Name:Tsen34
    Synonyms:Leng5, Sen34
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1913328. Tsen34.

    Subcellular locationi

    Nucleus By similarity. Nucleusnucleolus By similarity
    Note: May be transiently localized in the nucleolus.By similarity

    GO - Cellular componenti

    1. nucleolus Source: UniProtKB-SubCell
    2. tRNA-intron endonuclease complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 316316tRNA-splicing endonuclease subunit Sen34PRO_0000109464Add
    BLAST

    Proteomic databases

    PaxDbiQ8BMZ5.
    PRIDEiQ8BMZ5.

    PTM databases

    PhosphoSiteiQ8BMZ5.

    Expressioni

    Gene expression databases

    BgeeiQ8BMZ5.
    GenevestigatoriQ8BMZ5.

    Interactioni

    Subunit structurei

    tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ8BMZ5. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8BMZ5.
    SMRiQ8BMZ5. Positions 203-312.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    GeneTreeiENSGT00390000003912.
    HOGENOMiHOG000232197.
    HOVERGENiHBG054846.
    InParanoidiQ8BMZ5.
    KOiK15323.
    OMAiRTFSIEW.
    OrthoDBiEOG75XGMQ.
    PhylomeDBiQ8BMZ5.
    TreeFamiTF314631.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8BMZ5-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLVVEVANGR SLVWGAEAVQ ALRERLGVGG RTVGALPRGP RQNSRLGLPL    50
    LLLPEEARLL AEIGAVTLVS APRPDPRNHG LALASFKRQQ EQSFQDQNTL 100
    AAEARETRRQ ELLEKIVEGQ AAKKQKLEQD SGADEGGQEA GGSEATQGSE 150
    TSDDGQPSAE QEGAAPSLDS SSPQPGPSNG VTPLPRSALL IQLATARPRP 200
    VKAKPLDWRV QSKDWPHAGR PAHELRYSIY RDLWERGFFL SAAGKFGGDF 250
    LVYPGDPLRF HAHYIAQCWS AEDPIPLQDL VSAGRLGTSV RKTLLLCSPQ 300
    PDGKVVYTSL QWASLQ 316
    Length:316
    Mass (Da):34,196
    Last modified:July 19, 2004 - v2
    Checksum:i86A169BC119F7AC3
    GO
    Isoform 2 (identifier: Q8BMZ5-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         255-283: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:287
    Mass (Da):30,964
    Checksum:i0692A1E911014158
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201Q → L in BAB28747. (PubMed:16141072)Curated
    Sequence conflicti280 – 2801L → Q in BAB28747. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei255 – 28329Missing in isoform 2. 1 PublicationVSP_010987Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK003757 mRNA. Translation: BAC25051.1.
    AK013253 mRNA. Translation: BAB28747.1.
    AK053916 mRNA. Translation: BAC35591.1.
    BC002205 mRNA. Translation: AAH02205.1.
    BC091756 mRNA. Translation: AAH91756.1.
    CCDSiCCDS20725.1. [Q8BMZ5-1]
    CCDS51966.1. [Q8BMZ5-2]
    RefSeqiNP_001157676.1. NM_001164204.1. [Q8BMZ5-1]
    NP_001157677.1. NM_001164205.1. [Q8BMZ5-2]
    NP_077130.1. NM_024168.2. [Q8BMZ5-1]
    XP_006540345.1. XM_006540282.1. [Q8BMZ5-1]
    XP_006540346.1. XM_006540283.1. [Q8BMZ5-1]
    XP_006540347.1. XM_006540284.1. [Q8BMZ5-1]
    XP_006540348.1. XM_006540285.1. [Q8BMZ5-1]
    UniGeneiMm.379100.

    Genome annotation databases

    EnsembliENSMUST00000108627; ENSMUSP00000104267; ENSMUSG00000035585. [Q8BMZ5-1]
    ENSMUST00000108629; ENSMUSP00000104269; ENSMUSG00000035585. [Q8BMZ5-2]
    ENSMUST00000108630; ENSMUSP00000104270; ENSMUSG00000035585. [Q8BMZ5-1]
    GeneIDi66078.
    KEGGimmu:66078.
    UCSCiuc009evu.2. mouse. [Q8BMZ5-2]
    uc009evv.2. mouse. [Q8BMZ5-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK003757 mRNA. Translation: BAC25051.1 .
    AK013253 mRNA. Translation: BAB28747.1 .
    AK053916 mRNA. Translation: BAC35591.1 .
    BC002205 mRNA. Translation: AAH02205.1 .
    BC091756 mRNA. Translation: AAH91756.1 .
    CCDSi CCDS20725.1. [Q8BMZ5-1 ]
    CCDS51966.1. [Q8BMZ5-2 ]
    RefSeqi NP_001157676.1. NM_001164204.1. [Q8BMZ5-1 ]
    NP_001157677.1. NM_001164205.1. [Q8BMZ5-2 ]
    NP_077130.1. NM_024168.2. [Q8BMZ5-1 ]
    XP_006540345.1. XM_006540282.1. [Q8BMZ5-1 ]
    XP_006540346.1. XM_006540283.1. [Q8BMZ5-1 ]
    XP_006540347.1. XM_006540284.1. [Q8BMZ5-1 ]
    XP_006540348.1. XM_006540285.1. [Q8BMZ5-1 ]
    UniGenei Mm.379100.

    3D structure databases

    ProteinModelPortali Q8BMZ5.
    SMRi Q8BMZ5. Positions 203-312.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8BMZ5. 1 interaction.

    PTM databases

    PhosphoSitei Q8BMZ5.

    Proteomic databases

    PaxDbi Q8BMZ5.
    PRIDEi Q8BMZ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000108627 ; ENSMUSP00000104267 ; ENSMUSG00000035585 . [Q8BMZ5-1 ]
    ENSMUST00000108629 ; ENSMUSP00000104269 ; ENSMUSG00000035585 . [Q8BMZ5-2 ]
    ENSMUST00000108630 ; ENSMUSP00000104270 ; ENSMUSG00000035585 . [Q8BMZ5-1 ]
    GeneIDi 66078.
    KEGGi mmu:66078.
    UCSCi uc009evu.2. mouse. [Q8BMZ5-2 ]
    uc009evv.2. mouse. [Q8BMZ5-1 ]

    Organism-specific databases

    CTDi 79042.
    MGIi MGI:1913328. Tsen34.

    Phylogenomic databases

    eggNOGi COG1676.
    GeneTreei ENSGT00390000003912.
    HOGENOMi HOG000232197.
    HOVERGENi HBG054846.
    InParanoidi Q8BMZ5.
    KOi K15323.
    OMAi RTFSIEW.
    OrthoDBi EOG75XGMQ.
    PhylomeDBi Q8BMZ5.
    TreeFami TF314631.

    Miscellaneous databases

    NextBioi 320564.
    PROi Q8BMZ5.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8BMZ5.
    Genevestigatori Q8BMZ5.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Embryo and Oviduct.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Salivary gland.

    Entry informationi

    Entry nameiSEN34_MOUSE
    AccessioniPrimary (citable) accession number: Q8BMZ5
    Secondary accession number(s): Q58EU2, Q99LV6, Q9CYW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2004
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3