Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA-splicing endonuclease subunit Sen34

Gene

Tsen34

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei253By similarity1
Active sitei261By similarity1
Active sitei292By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processmRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen34 (EC:4.6.1.16)
Alternative name(s):
Leukocyte receptor cluster member 5 homolog
tRNA-intron endonuclease Sen34
Gene namesi
Name:Tsen34
Synonyms:Leng5, Sen34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1913328 Tsen34

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094641 – 316tRNA-splicing endonuclease subunit Sen34Add BLAST316

Proteomic databases

EPDiQ8BMZ5
PaxDbiQ8BMZ5
PeptideAtlasiQ8BMZ5
PRIDEiQ8BMZ5

PTM databases

PhosphoSitePlusiQ8BMZ5

Expressioni

Gene expression databases

BgeeiENSMUSG00000035585
ExpressionAtlasiQ8BMZ5 baseline and differential
GenevisibleiQ8BMZ5 MM

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of TSEN2, TSEN15, TSEN34/LENG5 and TSEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi211199, 1 interactor
IntActiQ8BMZ5, 1 interactor
MINTiQ8BMZ5
STRINGi10090.ENSMUSP00000104267

Structurei

3D structure databases

ProteinModelPortaliQ8BMZ5
SMRiQ8BMZ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiKOG4133 Eukaryota
COG1676 LUCA
GeneTreeiENSGT00390000003912
HOGENOMiHOG000232197
HOVERGENiHBG054846
InParanoidiQ8BMZ5
KOiK15323
OMAiRTFSIEW
OrthoDBiEOG091G0R5G
PhylomeDBiQ8BMZ5
TreeFamiTF314631

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006676 tRNA_splic
IPR016690 tRNA_splic_SEN34
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PIRSFiPIRSF017250 tRNA_splic_SEN34, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8BMZ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVVEVANGR SLVWGAEAVQ ALRERLGVGG RTVGALPRGP RQNSRLGLPL
60 70 80 90 100
LLLPEEARLL AEIGAVTLVS APRPDPRNHG LALASFKRQQ EQSFQDQNTL
110 120 130 140 150
AAEARETRRQ ELLEKIVEGQ AAKKQKLEQD SGADEGGQEA GGSEATQGSE
160 170 180 190 200
TSDDGQPSAE QEGAAPSLDS SSPQPGPSNG VTPLPRSALL IQLATARPRP
210 220 230 240 250
VKAKPLDWRV QSKDWPHAGR PAHELRYSIY RDLWERGFFL SAAGKFGGDF
260 270 280 290 300
LVYPGDPLRF HAHYIAQCWS AEDPIPLQDL VSAGRLGTSV RKTLLLCSPQ
310
PDGKVVYTSL QWASLQ
Length:316
Mass (Da):34,196
Last modified:July 19, 2004 - v2
Checksum:i86A169BC119F7AC3
GO
Isoform 2 (identifier: Q8BMZ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     255-283: Missing.

Note: No experimental confirmation available.
Show »
Length:287
Mass (Da):30,964
Checksum:i0692A1E911014158
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20Q → L in BAB28747 (PubMed:16141072).Curated1
Sequence conflicti280L → Q in BAB28747 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010987255 – 283Missing in isoform 2. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003757 mRNA Translation: BAC25051.1
AK013253 mRNA Translation: BAB28747.1
AK053916 mRNA Translation: BAC35591.1
BC002205 mRNA Translation: AAH02205.1
BC091756 mRNA Translation: AAH91756.1
CCDSiCCDS20725.1 [Q8BMZ5-1]
CCDS51966.1 [Q8BMZ5-2]
RefSeqiNP_001157676.1, NM_001164204.1 [Q8BMZ5-1]
NP_001157677.1, NM_001164205.1 [Q8BMZ5-2]
NP_077130.1, NM_024168.2 [Q8BMZ5-1]
XP_017167711.1, XM_017312222.1 [Q8BMZ5-1]
XP_017167712.1, XM_017312223.1 [Q8BMZ5-1]
XP_017167713.1, XM_017312224.1 [Q8BMZ5-1]
XP_017167714.1, XM_017312225.1 [Q8BMZ5-1]
XP_017167715.1, XM_017312226.1 [Q8BMZ5-1]
UniGeneiMm.379100
Mm.439946

Genome annotation databases

EnsembliENSMUST00000108627; ENSMUSP00000104267; ENSMUSG00000035585 [Q8BMZ5-1]
ENSMUST00000108629; ENSMUSP00000104269; ENSMUSG00000035585 [Q8BMZ5-2]
ENSMUST00000108630; ENSMUSP00000104270; ENSMUSG00000035585 [Q8BMZ5-1]
GeneIDi66078
KEGGimmu:66078
UCSCiuc009evu.2 mouse [Q8BMZ5-2]
uc009evv.2 mouse [Q8BMZ5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSEN34_MOUSE
AccessioniPrimary (citable) accession number: Q8BMZ5
Secondary accession number(s): Q58EU2, Q99LV6, Q9CYW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: April 25, 2018
This is version 120 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health