Q8BMZ5 (SEN34_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease subunit Sen34
EC=3.1.27.9

Alternative name(s):
Leukocyte receptor cluster member 5 homolog
tRNA-intron endonuclease Sen34

Gene names

Name:	Tsen34
Synonyms:	Leng5, Sen34

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Subunit structure	tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54. tRNA splicing endonuclease complex also contains proteins of the pre-mRNA 3'-end processing machinery such as CLP1, CPSF1, CPSF4 and CSTF2 By similarity.
Subcellular location	Nucleus By similarity. Nucleus › nucleolus By similarity. Note: May be transiently localized in the nucleolus By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
Biological process	mRNA processing tRNA processing
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA-type intron splice site recognition and cleavage Inferred from electronic annotation. Source: InterPro
Cellular_component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell tRNA-intron endonuclease complex Inferred from electronic annotation. Source: InterPro
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BMZ5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BMZ5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     255-283: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	tRNA-splicing endonuclease subunit Sen34		PRO_0000109464
Sites
	Active site	253	1	By similarity
	Active site	261	1	By similarity
	Active site	292	1	By similarity
Natural variations
	Alternative sequence	255 – 283	29	Missing in isoform 2.		VSP_010987
Experimental info
	Sequence conflict	20	1	Q → L in BAB28747. Ref.1
	Sequence conflict	280	1	L → Q in BAB28747. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 86A169BC119F7AC3	FASTA	316	34,196
	10 20 30 40 50 60 MLVVEVANGR SLVWGAEAVQ ALRERLGVGG RTVGALPRGP RQNSRLGLPL LLLPEEARLL 70 80 90 100 110 120 AEIGAVTLVS APRPDPRNHG LALASFKRQQ EQSFQDQNTL AAEARETRRQ ELLEKIVEGQ 130 140 150 160 170 180 AAKKQKLEQD SGADEGGQEA GGSEATQGSE TSDDGQPSAE QEGAAPSLDS SSPQPGPSNG 190 200 210 220 230 240 VTPLPRSALL IQLATARPRP VKAKPLDWRV QSKDWPHAGR PAHELRYSIY RDLWERGFFL 250 260 270 280 290 300 SAAGKFGGDF LVYPGDPLRF HAHYIAQCWS AEDPIPLQDL VSAGRLGTSV RKTLLLCSPQ 310 PDGKVVYTSL QWASLQ
	Isoform 2 [UniParc]. Checksum: 0692A1E911014158	FASTA	287	30,964
	10 20 30 40 50 60 MLVVEVANGR SLVWGAEAVQ ALRERLGVGG RTVGALPRGP RQNSRLGLPL LLLPEEARLL 70 80 90 100 110 120 AEIGAVTLVS APRPDPRNHG LALASFKRQQ EQSFQDQNTL AAEARETRRQ ELLEKIVEGQ 130 140 150 160 170 180 AAKKQKLEQD SGADEGGQEA GGSEATQGSE TSDDGQPSAE QEGAAPSLDS SSPQPGPSNG 190 200 210 220 230 240 VTPLPRSALL IQLATARPRP VKAKPLDWRV QSKDWPHAGR PAHELRYSIY RDLWERGFFL 250 260 270 280 SAAGKFGGDF LVYPGRLGTS VRKTLLLCSP QPDGKVVYTS LQWASLQ

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Embryo and Oviduct.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Salivary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK003757 mRNA. Translation: BAC25051.1. AK013253 mRNA. Translation: BAB28747.1. AK053916 mRNA. Translation: BAC35591.1. BC002205 mRNA. Translation: AAH02205.1. BC091756 mRNA. Translation: AAH91756.1.
IPI	IPI00320543. IPI00453497.
RefSeq	NP_001157676.1. NM_001164204.1. NP_001157677.1. NM_001164205.1. NP_077130.1. NM_024168.2.
UniGene	Mm.379100.
3D structure databases
ProteinModelPortal	Q8BMZ5.
SMR	Q8BMZ5. Positions 203-312.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q8BMZ5. 1 interaction.
PTM databases
PhosphoSite	Q8BMZ5.
Proteomic databases
PaxDb	Q8BMZ5.
PRIDE	Q8BMZ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000108627; ENSMUSP00000104267; ENSMUSG00000035585. ENSMUST00000108629; ENSMUSP00000104269; ENSMUSG00000035585. ENSMUST00000108630; ENSMUSP00000104270; ENSMUSG00000035585.
GeneID	66078.
KEGG	mmu:66078.
UCSC	uc009evu.2. mouse. uc009evv.2. mouse.
Organism-specific databases
CTD	79042.
MGI	MGI:1913328. Tsen34.
Phylogenomic databases
eggNOG	COG1676.
GeneTree	ENSGT00390000003912.
HOGENOM	HOG000232197.
HOVERGEN	HBG054846.
InParanoid	Q8BMZ5.
KO	K15323.
OMA	DPRQHSL.
OrthoDB	EOG479F7J.
Gene expression databases
ArrayExpress	Q8BMZ5.
Bgee	Q8BMZ5.
Genevestigator	Q8BMZ5.
GermOnline	ENSMUSG00000035585. Mus musculus.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006676. tRNA_splic. IPR016690. tRNA_splic_SEN34. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. [Graphical view]
PIRSF	PIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...
Other
NextBio	320564.
SOURCE	Search...

Entry information

Entry name

SEN34_MOUSE

Accession

Primary (citable) accession number: Q8BMZ5
Secondary accession number(s): Q58EU2, Q99LV6, Q9CYW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program