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Protein

Serine protease inhibitor Kazal-type 2

Gene

Spink2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits trypsin in a dose-dependent manner. Required for maintenance of normal spermatogenesis. May be involved in regulating serine protease-dependent germ cell apoptosis.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei48 – 49Reactive bondPROSITE-ProRule annotation2

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • fertilization Source: MGI
  • male germ cell proliferation Source: MGI
  • male gonad development Source: MGI
  • negative regulation of serine-type endopeptidase activity Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • regulation of acrosome reaction Source: GO_Central
  • seminiferous tubule development Source: MGI
  • spermatid development Source: MGI
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Biological processi

Apoptosis, Differentiation, Spermatogenesis

Protein family/group databases

MEROPSiI01.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 2
Gene namesi
Name:Spink2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1917232. Spink2.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: MGI
  • cytoplasm Source: MGI
  • extracellular space Source: GO_Central
  • neuron projection Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000001656317 – 86Serine protease inhibitor Kazal-type 2Add BLAST70

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi38 ↔ 68PROSITE-ProRule annotation
Disulfide bondi46 ↔ 65PROSITE-ProRule annotation
Disulfide bondi54 ↔ 86PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ8BMY7.
PeptideAtlasiQ8BMY7.
PRIDEiQ8BMY7.

PTM databases

PhosphoSitePlusiQ8BMY7.

Expressioni

Tissue specificityi

Expressed in sperm (at protein level). Expressed in testis but not in ovary, brain, heart, kidney or lung. Within testis, expressed in epididymis and germ cells.2 Publications

Developmental stagei

First expressed at 16 days postpartum (dpp). Level increases until 20 dpp and is maintained into adulthood (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000053030.
CleanExiMM_SPINK2.
ExpressionAtlasiQ8BMY7. baseline and differential.
GenevisibleiQ8BMY7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067117.

Structurei

3D structure databases

ProteinModelPortaliQ8BMY7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 86Kazal-likePROSITE-ProRule annotationAdd BLAST55

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00530000064285.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiQ8BMY7.
OMAiEDGSHIN.
OrthoDBiEOG091G1BGL.
PhylomeDBiQ8BMY7.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BMY7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLVLLLLV TDFAASHETL DSSDSQIMKR SQFRTPDCGH FDFPACPRNL
60 70 80
NPVCGTDMNT YSNECTLCMK IREDGSHINI IKDEPC
Length:86
Mass (Da):9,722
Last modified:March 1, 2003 - v1
Checksum:i09C26C56B9CB745E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005709 mRNA. Translation: BAC25122.1.
BC087876 mRNA. Translation: AAH87876.1.
CCDSiCCDS39121.1.
RefSeqiNP_001276697.1. NM_001289768.1.
NP_899107.1. NM_183284.3.
UniGeneiMm.46106.

Genome annotation databases

EnsembliENSMUST00000065216; ENSMUSP00000067117; ENSMUSG00000053030.
GeneIDi69982.
KEGGimmu:69982.
UCSCiuc008xvx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005709 mRNA. Translation: BAC25122.1.
BC087876 mRNA. Translation: AAH87876.1.
CCDSiCCDS39121.1.
RefSeqiNP_001276697.1. NM_001289768.1.
NP_899107.1. NM_183284.3.
UniGeneiMm.46106.

3D structure databases

ProteinModelPortaliQ8BMY7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000067117.

Protein family/group databases

MEROPSiI01.012.

PTM databases

PhosphoSitePlusiQ8BMY7.

Proteomic databases

PaxDbiQ8BMY7.
PeptideAtlasiQ8BMY7.
PRIDEiQ8BMY7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065216; ENSMUSP00000067117; ENSMUSG00000053030.
GeneIDi69982.
KEGGimmu:69982.
UCSCiuc008xvx.2. mouse.

Organism-specific databases

CTDi6691.
MGIiMGI:1917232. Spink2.

Phylogenomic databases

eggNOGiKOG3649. Eukaryota.
ENOG410YC3T. LUCA.
GeneTreeiENSGT00530000064285.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiQ8BMY7.
OMAiEDGSHIN.
OrthoDBiEOG091G1BGL.
PhylomeDBiQ8BMY7.

Miscellaneous databases

PROiQ8BMY7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000053030.
CleanExiMM_SPINK2.
ExpressionAtlasiQ8BMY7. baseline and differential.
GenevisibleiQ8BMY7. MM.

Family and domain databases

InterProiIPR002350. Kazal_dom.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiISK2_MOUSE
AccessioniPrimary (citable) accession number: Q8BMY7
Secondary accession number(s): Q5M8S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

On the 2D-gel the determined pI of this protein is: 5, its MW is: 8 kDa.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.