Q8BMS1 (ECHA_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 95.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Trifunctional enzyme subunit alpha, mitochondrial Alternative name(s): TP-alpha | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 763 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Bifunctional subunit. |
| Catalytic activity | (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. |
| Pathway | |
| Subunit structure | Octamer of 4 alpha (HADHA) and 4 beta (HADHB) subunits By similarity. |
| Subcellular location | Mitochondrion matrix By similarity. |
| Post-translational modification | Acetylation of Lys-569 and Lys-728 is observed in liver mitochondria from fasted mice but not from fed mice. |
| Sequence similarities | In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Fatty acid metabolism Lipid metabolism |
| Cellular component | Mitochondrion |
| Domain | Transit peptide |
| Ligand | NAD |
| Molecular function | Lyase Oxidoreductase |
| PTM | Acetylation |
| Technical term | Complete proteome Multifunctional enzyme Reference proteome |
| Gene Ontology (GO) | |
| Biological process | fatty acid beta-oxidation Inferred from mutant phenotype. Source: MGI response to insulin stimulusInferred from mutant phenotype. Source: MGI |
| Cellular component | fatty acid beta-oxidation multienzyme complex Inferred from electronic annotation. Source: InterPro mitochondrial inner membraneInferred from direct assay. Source: MGI |
| Molecular function | enoyl-CoA hydratase activity Traceable author statement. Source: MGI long-chain-3-hydroxyacyl-CoA dehydrogenase activityInferred from direct assay. Source: MGI |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 36 | 36 | Mitochondrion Potential | ||||||
| Chain | 37 – 763 | 727 | Trifunctional enzyme subunit alpha, mitochondrial | PRO_0000322639 | |||||
Sites | |||||||||
| Site | 151 | 1 | Important for catalytic activity By similarity | ||||||
| Site | 173 | 1 | Important for catalytic activity By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 129 | 1 | N6-acetyllysine Ref.3 | ||||||
| Modified residue | 295 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 303 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 326 | 1 | N6-acetyllysine Ref.3 | ||||||
| Modified residue | 350 | 1 | N6-acetyllysine Ref.3 | ||||||
| Modified residue | 353 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 359 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 406 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 505 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 540 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 569 | 1 | N6-acetyllysine Ref.3 | ||||||
| Modified residue | 644 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 728 | 1 | N6-acetyllysine Ref.3 | ||||||
Experimental info | |||||||||
| Sequence conflict | 196 | 1 | A → D in BAE41822. Ref.1 | ||||||
| Sequence conflict | 459 | 1 | L → S in AAH37009. Ref.2 | ||||||
Sequences
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References
| [1] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Skin. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Eye, Liver and Olfactory epithelium. |
| [3] | "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." Kim S.C., Sprung R., Chen Y., Xu Y., Ball H., Pei J., Cheng T., Kho Y., Xiao H., Xiao L., Grishin N.V., White M., Yang X.-J., Zhao Y. Mol. Cell 23:607-618(2006) [PubMed: 16916647] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-129; LYS-326; LYS-350; LYS-569 AND LYS-728, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK029017 mRNA. Translation: BAC26245.1. AK170478 mRNA. Translation: BAE41822.1. AK170683 mRNA. Translation: BAE41956.1. BC027156 mRNA. Translation: AAH27156.1. BC037009 mRNA. Translation: AAH37009.1. BC046978 mRNA. Translation: AAH46978.1. BC058569 mRNA. Translation: AAH58569.1. |
| IPI | IPI00223092. |
| RefSeq | NP_849209.1. NM_178878.2. |
| UniGene | Mm.200497. |
3D structure databases | |
| HSSP | HSSP built from PDB template 2DUB based on UniProtKB P14604. |
| ProteinModelPortal | Q8BMS1. |
| SMR | Q8BMS1. Positions 40-763. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q8BMS1. 3 interactions. |
| STRING | Q8BMS1. |
PTM databases | |
| PhosphoSite | Q8BMS1. |
2D gel databases | |
| REPRODUCTION-2DPAGE | IPI00223092. |
Proteomic databases | |
| PRIDE | Q8BMS1. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000156859; ENSMUSP00000120976; ENSMUSG00000025745. |
| GeneID | 97212. |
| KEGG | mmu:97212. |
| NMPDR | fig|10090.3.peg.11342. |
| UCSC | uc008wvc.1. mouse. |
Organism-specific databases | |
| CTD | 3030. |
| MGI | MGI:2135593. Hadha. |
Phylogenomic databases | |
| GeneTree | ENSGT00530000063042. |
| HOGENOM | HBG691737. |
| HOVERGEN | HBG005557. |
| InParanoid | Q8BMS1. |
| OMA | KQMVSKG. |
| OrthoDB | EOG4FBHSD. |
| PhylomeDB | Q8BMS1. |
Gene expression databases | |
| ArrayExpress | Q8BMS1. |
| Bgee | Q8BMS1. |
| Genevestigator | Q8BMS1. |
Family and domain databases | |
| InterPro | IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR008927. 6-PGluconate_DH_C-like. IPR001753. Crotonase_core. IPR013328. DH_multihelical. IPR018376. Enoyl-CoA_hyd/isom_CS. IPR012803. Fa_ox_alpha_mit. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 2 hits. |
| KO | K07515. |
| Pfam | PF00725. 3HCDH. 2 hits. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view] |
| SUPFAM | SSF48179. 6DGDH_C_like. 2 hits. |
| TIGRFAMs | TIGR02441. Fa_ox_alpha_mit. 1 hit. |
| PROSITE | PS00067. 3HCDH. 1 hit. PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 352627. |
| SOURCE | Search... |
Entry information
| Entry name | ECHA_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8BMS1 Secondary accession number(s): Q3TCY3, Q5U5Y5, Q8QZU4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with