Q8BMQ2 (TF3C4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General transcription factor 3C polypeptide 4
EC=2.3.1.48

Alternative name(s):
TF3C-delta
Transcription factor IIIC 90 kDa subunit
Short name=TFIIIC 90 kDa subunit
Short name=TFIIIC90
Transcription factor IIIC subunit delta

Gene names

Name:

Gtf3c4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	817 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box By similarity.
Catalytic activity	Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subunit structure	Part of the TFIIIC subcomplex TFIIIC2, consisting of six subunits, GTF3C1, GTF3C2, GTF3C3, GTF3C4, GTF3C5 and GTF3C6. Interacts with BRF1, GTF3C1, GTF3C2, GTF3C5, GTF3C6, POLR3C and POLR3F By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the TFIIIC subunit 4 family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone acetylation Inferred from electronic annotation. Source: GOC transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	transcription factor TFIIIC complex Inferred from electronic annotation. Source: Compara
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW histone acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8BMQ2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8BMQ2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-323: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8BMQ2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     270-817: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 817	817	General transcription factor 3C polypeptide 4		PRO_0000209714
Amino acid modifications
	Modified residue	607	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	1 – 323	323	Missing in isoform 2.		VSP_010577
	Alternative sequence	270 – 817	548	Missing in isoform 3.		VSP_010578
Experimental info
	Sequence conflict	21	1	E → Q in BAC26916. Ref.1
	Sequence conflict	703	1	P → H in BAC26916. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 895ED56D1B7B1F05	FASTA	817	91,613
	10 20 30 40 50 60 MSEADQALVG PKADEPSPPA EEKDEGGGKE AAADAAPGPS ASFRLMVTRR EPAVKLQYAV 70 80 90 100 110 120 SGLEPLSWSE DHRVSVSTAR SVAVLELICD VHNPGQDLVI HRTSVPAPLN SCLLKVGSKT 130 140 150 160 170 180 EVAECKEKFA SSKDPTISQT FMLDRMFNPE GKALPPMRGF KYTSWSPMGC DANGRCLLAA 190 200 210 220 230 240 LTMDNRLTVQ VNLNRLQWVQ LVDLTEIYGD RLYETSYRLS KNEAPEGNLG DFAEFQRRHS 250 260 270 280 290 300 MQTPVRMEWS SICTTQQVKH NNECRDVSSV LLAVLFENGN IAVWQFQLPF VGKESISSCN 310 320 330 340 350 360 TIESGISSPS VLFWWEYEHN NRKMSGLIVG SAFGPVKILP VNLKAVKGYF TLRQPVVLWK 370 380 390 400 410 420 EMDKLPVHSI KCVPLYHPYQ KCSCSLVVAA RGSYVFWCLL LISKAGLNVH NSHVTGLHSL 430 440 450 460 470 480 PIVSITADKQ NGTVYTCSSD GKVRQLIPIF TDVALKFEHQ LIKLSDVFGS VRTHGIAVSP 490 500 510 520 530 540 CGAYLAIITT EGMMNGLHPV NKNYQVQFVT LKTFEEAAAQ LLESSVQNLF KQVDLIDLVR 550 560 570 580 590 600 WKILKDKHIP QFLHEALEKK IESSGVTYFW RFKLFLLRIL YQSMQKSPSE ALWKPTHEDS 610 620 630 640 650 660 KILLVDSPGM GDGEDEQQEE GTSKQGTKAG LQEKSKEGDT EETPEDSLTA GGDTGGREPV 670 680 690 700 710 720 EEKLLEIQGK IEAVEMHLTR EHMKRVLGEV YLHTWITENT SIPTRGLCNF LMSDEDYDDR 730 740 750 760 770 780 TAQVLIGHIS KKMNKQTFPE RCSLCKEILP FTDRKQAVCS NGHIWLRCFL TYQSCQSLIY 790 800 810 RRCLLHDSIA RHPVPEDPDW IKRLLQSPCP FCDSPVF
	Isoform 2 [UniParc]. Checksum: 0B4138780B192F23	FASTA	494	55,746
	10 20 30 40 50 60 MSGLIVGSAF GPVKILPVNL KAVKGYFTLR QPVVLWKEMD KLPVHSIKCV PLYHPYQKCS 70 80 90 100 110 120 CSLVVAARGS YVFWCLLLIS KAGLNVHNSH VTGLHSLPIV SITADKQNGT VYTCSSDGKV 130 140 150 160 170 180 RQLIPIFTDV ALKFEHQLIK LSDVFGSVRT HGIAVSPCGA YLAIITTEGM MNGLHPVNKN 190 200 210 220 230 240 YQVQFVTLKT FEEAAAQLLE SSVQNLFKQV DLIDLVRWKI LKDKHIPQFL HEALEKKIES 250 260 270 280 290 300 SGVTYFWRFK LFLLRILYQS MQKSPSEALW KPTHEDSKIL LVDSPGMGDG EDEQQEEGTS 310 320 330 340 350 360 KQGTKAGLQE KSKEGDTEET PEDSLTAGGD TGGREPVEEK LLEIQGKIEA VEMHLTREHM 370 380 390 400 410 420 KRVLGEVYLH TWITENTSIP TRGLCNFLMS DEDYDDRTAQ VLIGHISKKM NKQTFPERCS 430 440 450 460 470 480 LCKEILPFTD RKQAVCSNGH IWLRCFLTYQ SCQSLIYRRC LLHDSIARHP VPEDPDWIKR 490 LLQSPCPFCD SPVF
	Isoform 3 [UniParc]. Checksum: 687FB95613A1607E	FASTA	269	29,717
	10 20 30 40 50 60 MSEADQALVG PKADEPSPPA EEKDEGGGKE AAADAAPGPS ASFRLMVTRR EPAVKLQYAV 70 80 90 100 110 120 SGLEPLSWSE DHRVSVSTAR SVAVLELICD VHNPGQDLVI HRTSVPAPLN SCLLKVGSKT 130 140 150 160 170 180 EVAECKEKFA SSKDPTISQT FMLDRMFNPE GKALPPMRGF KYTSWSPMGC DANGRCLLAA 190 200 210 220 230 240 LTMDNRLTVQ VNLNRLQWVQ LVDLTEIYGD RLYETSYRLS KNEAPEGNLG DFAEFQRRHS 250 260 MQTPVRMEWS SICTTQQVKH NNECRDVSS

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Corpus striatum and Pituitary.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Tissue: Limb.
[5]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-607, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK030351 mRNA. Translation: BAC26916.1. AK047698 mRNA. Translation: BAC33130.1. AL732526 Genomic DNA. Translation: CAM21459.1. CH466542 Genomic DNA. Translation: EDL08398.1. BC061476 mRNA. Translation: AAH61476.1. BC117919 mRNA. Translation: AAI17920.1. BC117920 mRNA. Translation: AAI17921.1.
IPI	IPI00223076. IPI00417149. IPI00417150.
RefSeq	NP_001159505.1. NM_001166033.1. NP_766565.2. NM_172977.3.
UniGene	Mm.26043.
3D structure databases
ProteinModelPortal	Q8BMQ2.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000042265.
PTM databases
PhosphoSite	Q8BMQ2.
Proteomic databases
PaxDb	Q8BMQ2.
PRIDE	Q8BMQ2.
Protocols and materials databases
DNASU	269252.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000037117; ENSMUSP00000042265; ENSMUSG00000035666.
GeneID	269252.
KEGG	mmu:269252.
UCSC	uc012btc.1. mouse.
Organism-specific databases
CTD	9329.
MGI	MGI:2138937. Gtf3c4.
Phylogenomic databases
eggNOG	NOG79304.
GeneTree	ENSGT00390000011873.
HOGENOM	HOG000121774.
HOVERGEN	HBG059337.
InParanoid	Q148Y1.
KO	K11310.
OMA	AVWQFQL.
OrthoDB	EOG40CHG9.
Gene expression databases
ArrayExpress	Q8BMQ2.
Bgee	Q8BMQ2.
CleanEx	MM_GTF3C4.
Genevestigator	Q8BMQ2.
GermOnline	ENSMUSG00000035666. Mus musculus.
Family and domain databases
InterPro	IPR024761. TFIIIC_delta_N. IPR024764. TFIIIC_Znf. [Graphical view]
Pfam	PF12657. TFIIIC_delta. 1 hit. PF12660. zf-TFIIIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	392756.
SOURCE	Search...

Entry information

Entry name

TF3C4_MOUSE

Accession

Primary (citable) accession number: Q8BMQ2
Secondary accession number(s): Q148Y1, Q8BKZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program