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Protein

Golgi resident protein GCP60

Gene

Acbd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi (By similarity). Involved in hormone-induced steroid biosynthesis in testicular Leydig cells (PubMed:12711385). Recruits PI4KB to the Golgi apparatus membrane; enhances the enzyme activity of PI4KB activity via its membrane recruitment thereby increasing the local concentration of the substrate in the vicinity of the kinase (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi resident protein GCP60
Alternative name(s):
Acyl-CoA-binding domain-containing protein 3
Golgi complex-associated protein 1
Short name:
GOCAP1
Golgi phosphoprotein 1
Short name:
GOLPH1
PBR- and PKA-associated protein 7
Peripheral benzodiazepine receptor-associated protein PAP7
Gene namesi
Name:Acbd3
Synonyms:Gcp60, Pap7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2181074. Acbd3.

Subcellular locationi

  • Golgi apparatus membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
  • Mitochondrion 1 Publication

  • Note: Also mitochondrial (via its interaction with PBR).

GO - Cellular componenti

Keywords - Cellular componenti

Golgi apparatus, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004364502 – 525Golgi resident protein GCP60Add BLAST524

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei18PhosphothreonineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Modified residuei40PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8BMP6.
MaxQBiQ8BMP6.
PaxDbiQ8BMP6.
PeptideAtlasiQ8BMP6.
PRIDEiQ8BMP6.

PTM databases

iPTMnetiQ8BMP6.
PhosphoSitePlusiQ8BMP6.

Expressioni

Tissue specificityi

Expressed in brain (hippocampus, olfactory bulb, neuronal and glial cells of the cortex), eye, submaxillary gland, testis (interstitial and tubular compartments), ovary (granulosa cells, theca cells at late stages and primary follicles), adrenal gland (fasciculata and glomerulosa cells), heart, liver, and steroidogenic cell lines.1 Publication

Developmental stagei

Present in embryo. Decreases before birth.1 Publication

Gene expression databases

CleanExiMM_ACBD3.

Interactioni

Subunit structurei

Interacts with the C-terminal cytoplasmic domain of giantin/GOLGB1 (By similarity). Interacts with PBR and PKA regulatory subunit RI-alpha. Does not interact with PKA regulatory subunit RI-beta nor PKA regulatory subunit RII-alpha (PubMed:11731621). Interacts with PI4KB, TBC1D22A AND TBC1D22B; interactions with PI4KB and with TBC1D22A and TBC1D22B are mutually exclusive. Interacts with C10ORF76 and RAB11B (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-1858691.
STRINGi10090.ENSMUSP00000027780.

Structurei

3D structure databases

ProteinModelPortaliQ8BMP6.
SMRiQ8BMP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 171ACBPROSITE-ProRule annotationAdd BLAST92
Domaini381 – 523GOLDPROSITE-ProRule annotationAdd BLAST143

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 305Interaction with PI4KBBy similarityAdd BLAST68

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili169 – 254Sequence analysisAdd BLAST86
Coiled coili448 – 470Sequence analysisAdd BLAST23

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi179 – 184Poly-Glu6
Compositional biasi186 – 235Arg-richAdd BLAST50
Compositional biasi238 – 305Gln-richAdd BLAST68

Domaini

The GOLD domain is essential for giantin binding.By similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3878. Eukaryota.
ENOG410XS5V. LUCA.
HOGENOMiHOG000247039.
HOVERGENiHBG051712.
InParanoidiQ8BMP6.
PhylomeDBiQ8BMP6.
TreeFamiTF321667.

Family and domain databases

Gene3Di1.20.80.10. 1 hit.
InterProiView protein in InterPro
IPR022408. Acyl-CoA-binding_prot_CS.
IPR000582. Acyl-CoA-binding_protein.
IPR014352. FERM/acyl-CoA-bd_prot_3-hlx.
IPR009038. GOLD_dom.
PfamiView protein in Pfam
PF00887. ACBP. 1 hit.
PF13897. GOLD_2. 1 hit.
SUPFAMiSSF101576. SSF101576. 2 hits.
SSF47027. SSF47027. 1 hit.
PROSITEiView protein in PROSITE
PS00880. ACB_1. 1 hit.
PS51228. ACB_2. 1 hit.
PS50866. GOLD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8BMP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQLNVEQL EVSLDGLTLS PDSEERPGAE GAPPQTPPSS APGNGLGSGA
60 70 80 90 100
SGQQREPGEA AAEGAAEEAR RMEQHWGFGL EELYGLALRF YKIKDGKAFH
110 120 130 140 150
PTYEEKLKFV ALHKQVLLGP YNPDTSPEVG FFDVLGNDRR REWAALGNMS
160 170 180 190 200
KEDAMVEFVK LLNKCCPLLS AYVASHRIEK EEEEKRRKAE EERRQREEEE
210 220 230 240 250
RERLQKEEEK RKREKEDRLR REEEERRRIE EERLRLEQQK QQIMAALNSQ
260 270 280 290 300
TAVQFQQYAA QQYPGNYEQQ QILIRQLQEQ HYQQYMQQLY QVQLAQQQAA
310 320 330 340 350
LQKQQEVVMA GASLPASSKV NTAGASDTLS VNGQAKTHTE NSEKVLEPEA
360 370 380 390 400
AEEALENGPK DSLPVIAAPS MWTRPQIKDF KEKIRQDADS VITVRRGEVV
410 420 430 440 450
TVRVPTHEEG SYLFWEFATD SYDIGFGVYF EWTDSPNAAV SVHVSESSDE
460 470 480 490 500
EEEEEENVTC EEKAKKNANK PLLDEIVPVY RRDCHEEVYA GSHQYPGRGV
510 520
YLLKFDNSYS LWRSKSVYYR VYYTR
Length:525
Mass (Da):60,181
Last modified:January 23, 2007 - v3
Checksum:iC043CEBF56FCE702
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022770 mRNA. Translation: AAB71197.3.
AF501319 Genomic DNA. Translation: AAM22185.1.
AK030371 mRNA. Translation: BAC26928.1.
CCDSiCCDS15572.1.
RefSeqiNP_573488.2. NM_133225.3.
UniGeneiMm.272981.

Genome annotation databases

GeneIDi170760.
KEGGimmu:170760.
UCSCiuc007dwq.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiGCP60_MOUSE
AccessioniPrimary (citable) accession number: Q8BMP6
Secondary accession number(s): O35371
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 125 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot